LOCUS       NM_139222                349 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus defensin beta 15 (Defb15), mRNA.
ACCESSION   NM_139222
VERSION     NM_139222.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Amid,C., Rehaume,L.M., Brown,K.L., Gilbert,J.G., Dougan,G.,
            Hancock,R.E. and Harrow,J.L.
  TITLE     Manual annotation and analysis of the defensin gene cluster in the
            C57BL/6J mouse reference genome
  JOURNAL   BMC Genomics 10, 606 (2009)
   PUBMED   20003482
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  AUTHORS   Zaballos,A., Villares,R., Albar,J.P., Martinez-A,C. and Marquez,G.
  TITLE     Identification on mouse chromosome 8 of new beta-defensin genes
            with regionally specific expression in the male reproductive organ
  JOURNAL   J Biol Chem 279 (13), 12421-12426 (2004)
   PUBMED   14718547
REFERENCE   3  (bases 1 to 349)
  AUTHORS   Morrison,G.M., Semple,C.A., Kilanowski,F.M., Hill,R.E. and
            Dorin,J.R.
  TITLE     Signal sequence conservation and mature peptide divergence within
            subgroups of the murine beta-defensin gene family
  JOURNAL   Mol Biol Evol 20 (3), 460-470 (2003)
   PUBMED   12644567
REFERENCE   4  (bases 1 to 349)
  AUTHORS   Yamaguchi,Y., Nagase,T., Makita,R., Fukuhara,S., Tomita,T.,
            Tominaga,T., Kurihara,H. and Ouchi,Y.
  TITLE     Identification of multiple novel epididymis-specific beta-defensin
            isoforms in humans and mice
  JOURNAL   J Immunol 169 (5), 2516-2523 (2002)
   PUBMED   12193721
REFERENCE   5  (bases 1 to 349)
  AUTHORS   Schutte,B.C., Mitros,J.P., Bartlett,J.A., Walters,J.D., Jia,H.P.,
            Welsh,M.J., Casavant,T.L. and McCray,P.B. Jr.
  TITLE     Discovery of five conserved beta -defensin gene clusters using a
            computational search strategy
  JOURNAL   Proc Natl Acad Sci U S A 99 (4), 2129-2133 (2002)
   PUBMED   11854508
  REMARK    Erratum:[Proc Natl Acad Sci U S A 2002 Oct 29;99(22):14611]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL590619.9.
            
            On Sep 6, 2022 this sequence version replaced NM_139222.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ437648.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-85                AL590619.9         99930-100014
            86-349              AL590619.9         102582-102845
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 10.64 cM"
     gene            1..349
                     /gene="Defb15"
                     /gene_synonym="BD-15"
                     /note="defensin beta 15"
                     /db_xref="GeneID:246082"
                     /db_xref="MGI:MGI:2179202"
     exon            1..85
                     /gene="Defb15"
                     /gene_synonym="BD-15"
                     /inference="alignment:Splign:2.1.0"
     CDS             37..276
                     /gene="Defb15"
                     /gene_synonym="BD-15"
                     /function="antimicrobial peptide"
                     /note="beta-defensin 15"
                     /codon_start=1
                     /product="beta-defensin 15 precursor"
                     /protein_id="NP_631968.1"
                     /db_xref="CCDS:CCDS40288.1"
                     /db_xref="GeneID:246082"
                     /db_xref="MGI:MGI:2179202"
                     /translation="MKTFLFLFAVLFFLDPAKNAFFDEKCSRVNGRCTASCLKNEELV
                     ALCQKNLKCCVTVQPCGKSKSNQSDEGSGHMGTWG"
     exon            86..349
                     /gene="Defb15"
                     /gene_synonym="BD-15"
                     /inference="alignment:Splign:2.1.0"
     regulatory      327..332
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Defb15"
                     /gene_synonym="BD-15"
                     /note="hexamer: AATAAA"
     polyA_site      349
                     /gene="Defb15"
                     /gene_synonym="BD-15"
                     /note="major polyA site"
ORIGIN      
        1 atggacactg tgctgaccct cgcagccctt caggtcatga agacattctt atttctcttt
       61 gctgttctct tctttctgga cccagccaag aatgcatttt ttgatgagaa atgcagcagg
      121 gttaatggga gatgcacagc atcttgtctg aagaatgaag aactcgttgc tctctgccag
      181 aagaatctga agtgctgtgt gacagtccag ccctgtggaa agagcaagag caaccagtca
      241 gatgaaggct caggccacat ggggacttgg ggatgaacct ttgtcctttt gttgtggcac
      301 ctgatggaag cagatgcttc ggtgaaaata aatggctgct tttcttcaa
//
