LOCUS       NM_007850                410 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus defensin, alpha, 3 (Defa3), mRNA.
ACCESSION   NM_007850 XM_001006539 XM_980142
VERSION     NM_007850.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Chen,Q., Yang,Y., Hou,J., Shu,Q., Yin,Y., Fu,W., Han,F., Hou,T.,
            Zeng,C., Nemeth,E., Linzmeier,R., Ganz,T. and Fang,X.
  TITLE     Increased gene copy number of DEFA1/DEFA3 worsens sepsis by
            inducing endothelial pyroptosis
  JOURNAL   Proc Natl Acad Sci U S A 116 (8), 3161-3170 (2019)
   PUBMED   30718392
  REMARK    GeneRIF: we engineered a monoclonal antibody against HNP-1 to block
            the interaction with P2X7 and found that the blocking antibody
            protected mice carrying high copy number of DEFA1/DEFA3 from lethal
            sepsis. We thus demonstrate that DEFA1/DEFA3 copy number variation
            strongly modulates sepsis development in vivo and explore a
            paradigm for the precision treatment of sepsis tailored by
            individual genetic information.
            Erratum:[Proc Natl Acad Sci U S A. 2022 Jan 25;119(4):e2121889119.
            doi: 10.1073/pnas.2121889119.. PMID: 35074923]
REFERENCE   2  (bases 1 to 410)
  AUTHORS   Amid,C., Rehaume,L.M., Brown,K.L., Gilbert,J.G., Dougan,G.,
            Hancock,R.E. and Harrow,J.L.
  TITLE     Manual annotation and analysis of the defensin gene cluster in the
            C57BL/6J mouse reference genome
  JOURNAL   BMC Genomics 10, 606 (2009)
   PUBMED   20003482
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 410)
  AUTHORS   Nyeng,P., Norgaard,G.A., Kobberup,S. and Jensen,J.
  TITLE     FGF10 maintains distal lung bud epithelium and excessive signaling
            leads to progenitor state arrest, distalization, and goblet cell
            metaplasia
  JOURNAL   BMC Dev Biol 8, 2 (2008)
   PUBMED   18186922
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 410)
  AUTHORS   Yue,G., Merlin,D., Selsted,M.E., Lencer,W.I., Madara,J.L. and
            Eaton,D.C.
  TITLE     Cryptdin 3 forms anion selective channels in cytoplasmic membranes
            of human embryonic kidney cells
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 282 (5), G757-G765 (2002)
   PUBMED   11960772
  REMARK    GeneRIF: cryptdin 3 forms anion-selective channels on the
            cytoplasmic membrane of HEK cells and that the kinetics of one such
            channel are affected by its interaction with other such channels.
REFERENCE   5  (bases 1 to 410)
  AUTHORS   Ayabe,T., Satchell,D.P., Pesendorfer,P., Tanabe,H., Wilson,C.L.,
            Hagen,S.J. and Ouellette,A.J.
  TITLE     Activation of Paneth cell alpha-defensins in mouse small intestine
  JOURNAL   J Biol Chem 277 (7), 5219-5228 (2002)
   PUBMED   11733520
  REMARK    GeneRIF: MMP-7-dependent procryptdin activation in vivo provides
            mouse Paneth cells with functional peptides for apical secretion
            into the small intestine lumen
REFERENCE   6  (bases 1 to 410)
  AUTHORS   Jia,H.P., Wowk,S.A., Schutte,B.C., Lee,S.K., Vivado,A., Tack,B.F.,
            Bevins,C.L. and McCray,P.B. Jr.
  TITLE     A novel murine beta -defensin expressed in tongue, esophagus, and
            trachea
  JOURNAL   J Biol Chem 275 (43), 33314-33320 (2000)
   PUBMED   10922379
REFERENCE   7  (bases 1 to 410)
  AUTHORS   Ayabe,T., Satchell,D.P., Wilson,C.L., Parks,W.C., Selsted,M.E. and
            Ouellette,A.J.
  TITLE     Secretion of microbicidal alpha-defensins by intestinal Paneth
            cells in response to bacteria
  JOURNAL   Nat Immunol 1 (2), 113-118 (2000)
   PUBMED   11248802
REFERENCE   8  (bases 1 to 410)
  AUTHORS   Ouellette,A.J., Hsieh,M.M., Nosek,M.T., Cano-Gauci,D.F.,
            Huttner,K.M., Buick,R.N. and Selsted,M.E.
  TITLE     Mouse Paneth cell defensins: primary structures and antibacterial
            activities of numerous cryptdin isoforms
  JOURNAL   Infect Immun 62 (11), 5040-5047 (1994)
   PUBMED   7927786
REFERENCE   9  (bases 1 to 410)
  AUTHORS   Huttner,K.M., Selsted,M.E. and Ouellette,A.J.
  TITLE     Structure and diversity of the murine cryptdin gene family
  JOURNAL   Genomics 19 (3), 448-453 (1994)
   PUBMED   8188287
  REMARK    Erratum:[Genomics 1995 Feb 10;25(3):762]
REFERENCE   10 (bases 1 to 410)
  AUTHORS   Selsted,M.E., Miller,S.I., Henschen,A.H. and Ouellette,A.J.
  TITLE     Enteric defensins: antibiotic peptide components of intestinal host
            defense
  JOURNAL   J Cell Biol 118 (4), 929-936 (1992)
   PUBMED   1500431
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC129174.3.
            
            On Apr 14, 2010 this sequence version replaced NM_007850.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AA869181.1, BC099855.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-206               AC129174.3         68529-68734         c
            207-410             AC129174.3         67766-67969         c
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 10.35 cM"
     gene            1..410
                     /gene="Defa3"
                     /gene_synonym="Defa27; Defcr-3; Defcr3"
                     /note="defensin, alpha, 3"
                     /db_xref="GeneID:13237"
                     /db_xref="MGI:MGI:94883"
     exon            1..206
                     /gene="Defa3"
                     /gene_synonym="Defa27; Defcr-3; Defcr3"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..316
                     /gene="Defa3"
                     /gene_synonym="Defa27; Defcr-3; Defcr3"
                     /note="defensin-related cryptdin-3; alpha-defensin 3;
                     defensin, alpha, 27"
                     /codon_start=1
                     /product="alpha-defensin 3 precursor"
                     /protein_id="NP_031876.1"
                     /db_xref="CCDS:CCDS40270.1"
                     /db_xref="GeneID:13237"
                     /db_xref="MGI:MGI:94883"
                     /translation="MKTLVLLSALVLLAFQVQADPIQNTDEETKTEEQPGEDDQAVSV
                     SFGDPEGSSLQEESLRDLVCYCRKRGCKRRERMNGTCRKGHLMYTLCCR"
     exon            207..410
                     /gene="Defa3"
                     /gene_synonym="Defa27; Defcr-3; Defcr3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      391..396
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Defa3"
                     /gene_synonym="Defa27; Defcr-3; Defcr3"
                     /note="hexamer: AATAAA"
     polyA_site      410
                     /gene="Defa3"
                     /gene_synonym="Defa27; Defcr-3; Defcr3"
                     /note="major polyA site"
ORIGIN      
        1 tcctgctcac caatcctcca ggtgactccc agccatgaag acactagtcc tcctctctgc
       61 cctcgtcctg ctggccttcc aggtccaggc tgatcctatc caaaacacag atgaagagac
      121 taaaactgag gagcagccag gggaagacga ccaggctgtg tctgtctctt ttggagaccc
      181 agaaggctct tctcttcaag aggaatcgtt gagagatctg gtatgctatt gtagaaaaag
      241 aggctgcaaa agaagagaac gcatgaatgg gacctgcaga aagggtcatt taatgtacac
      301 actctgctgt cgctgaacat ggagaccaca gaggacaaga cgaacatgag tactgaggcc
      361 actgatgctg gtgcctgatg actacctcgc aataaattgt tcgcaatatg
//
