LOCUS       NM_183268                425 bp    mRNA    linear   ROD 03-APR-2024
DEFINITION  Mus musculus defensin, alpha, 20 (Defa20), mRNA.
ACCESSION   NM_183268
VERSION     NM_183268.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Shanahan,M.T., Tanabe,H. and Ouellette,A.J.
  TITLE     Strain-specific polymorphisms in Paneth cell alpha-defensins of
            C57BL/6 mice and evidence of vestigial myeloid alpha-defensin
            pseudogenes
  JOURNAL   Infect Immun 79 (1), 459-473 (2011)
   PUBMED   21041494
REFERENCE   2  (bases 1 to 425)
  AUTHORS   Amid,C., Rehaume,L.M., Brown,K.L., Gilbert,J.G., Dougan,G.,
            Hancock,R.E. and Harrow,J.L.
  TITLE     Manual annotation and analysis of the defensin gene cluster in the
            C57BL/6J mouse reference genome
  JOURNAL   BMC Genomics 10, 606 (2009)
   PUBMED   20003482
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 425)
  AUTHORS   Figueredo,S.M., Weeks,C.S., Young,S.K. and Ouellette,A.J.
  TITLE     Anionic amino acids near the pro-alpha-defensin N terminus mediate
            inhibition of bactericidal activity in mouse pro-cryptdin-4
  JOURNAL   J Biol Chem 284 (11), 6826-6831 (2009)
   PUBMED   19106102
  REMARK    GeneRIF: the most distal N-terminal anionic residues of
            pro-Crp4-(20-92) are primarily responsible for blocking
            Crp4-mediated membrane disruption in the precursor.
REFERENCE   4  (bases 1 to 425)
  AUTHORS   Patil,A., Hughes,A.L. and Zhang,G.
  TITLE     Rapid evolution and diversification of mammalian alpha-defensins as
            revealed by comparative analysis of rodent and primate genes
  JOURNAL   Physiol Genomics 20 (1), 1-11 (2004)
   PUBMED   15494476
REFERENCE   5  (bases 1 to 425)
  AUTHORS   Maemoto,A., Qu,X., Rosengren,K.J., Tanabe,H., Henschen-Edman,A.,
            Craik,D.J. and Ouellette,A.J.
  TITLE     Functional analysis of the alpha-defensin disulfide array in mouse
            cryptdin-4
  JOURNAL   J Biol Chem 279 (42), 44188-44196 (2004)
   PUBMED   15297466
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK008275.1.
            
            On May 4, 2013 this sequence version replaced NM_183268.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK008275.1, AK008107.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-425               AK008275.1         2-426
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 10.35 cM"
     gene            1..425
                     /gene="Defa20"
                     /gene_synonym="2010016F14Rik; Defcr20"
                     /note="defensin, alpha, 20"
                     /db_xref="GeneID:68009"
                     /db_xref="MGI:MGI:1915259"
     exon            1..215
                     /gene="Defa20"
                     /gene_synonym="2010016F14Rik; Defcr20"
                     /inference="alignment:Splign:2.1.0"
     CDS             44..331
                     /gene="Defa20"
                     /gene_synonym="2010016F14Rik; Defcr20"
                     /note="defensin-related cryptdin-20; cryptdin4;
                     alpha-defensin 20; cryptdin-4"
                     /codon_start=1
                     /product="alpha-defensin 20 precursor"
                     /protein_id="NP_899091.2"
                     /db_xref="CCDS:CCDS40273.1"
                     /db_xref="GeneID:68009"
                     /db_xref="MGI:MGI:1915259"
                     /translation="MKTLVLLSALVLLAFQVQADPIQNTDEETNTEEQPGEEDQAVSV
                     SFGDPEGSALHEKSSRDLICYCRKGGCNRGEQVYGTCSGRLLFCCRRRHRH"
     exon            216..425
                     /gene="Defa20"
                     /gene_synonym="2010016F14Rik; Defcr20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      405..410
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Defa20"
                     /gene_synonym="2010016F14Rik; Defcr20"
                     /note="putative"
     polyA_site      425
                     /gene="Defa20"
                     /gene_synonym="2010016F14Rik; Defcr20"
                     /note="putative"
ORIGIN      
        1 acattgggct cctgctcacc aattctccag gtgactcaca gccatgaaga cacttgtcct
       61 cctctctgcc ctcgtcctgc tggccttcca ggtccaggct gatcctatcc aaaacacaga
      121 tgaggagact aatactgagg agcagccagg ggaggaggac caggctgtgt ctgtctcctt
      181 tggagaccca gaaggatctg ctcttcatga aaaatcgtcg agagatctga tatgctattg
      241 tagaaaagga ggctgcaata gaggagaaca agtttatggg acctgctcag gacgactttt
      301 gttctgctgc cgccgccgcc accgccactg aacatgcaga tgacagagat atgacaacca
      361 tcagctctga ggctactgat gctggggcct gataaccact tctcaataaa ttgtttgcaa
      421 tatgc
//
