LOCUS       NM_053183               2758 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus DExD box helicase 50 (Ddx50), transcript variant 1,
            mRNA.
ACCESSION   NM_053183
VERSION     NM_053183.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Valdez,B.C., Yang,H., Hong,E. and Sequitin,A.M.
  TITLE     Genomic structure of newly identified paralogue of RNA helicase
            II/Gu: detection of pseudogenes and multiple alternatively spliced
            mRNAs
  JOURNAL   Gene 284 (1-2), 53-61 (2002)
   PUBMED   11891046
REFERENCE   3  (bases 1 to 2758)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121961.2, AK162423.1 and AK041281.1.
            
            On Dec 9, 2017 this sequence version replaced NM_053183.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC090996.1, AF334104.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AC121961.2         159349-159363       c
            16-194              AK162423.1         3-181
            195-296             AK162423.1         292-393
            297-2758            AK041281.1         176-2637
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 32.44 cM"
     gene            1..2758
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /note="DExD box helicase 50"
                     /db_xref="GeneID:94213"
                     /db_xref="MGI:MGI:2182303"
     exon            1..194
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     CDS             108..2312
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /EC_number="3.6.4.13"
                     /note="isoform 1 is encoded by transcript variant 1;
                     ATP-dependent RNA helicase DDX50; gu-beta; DEAD box
                     protein 50; RH-II/Gubeta; DEAD/H (Asp-Glu-Ala-Asp/His) box
                     polypeptide 50; nucleolar protein GU2; DEAD
                     (Asp-Glu-Ala-Asp) box polypeptide 50"
                     /codon_start=1
                     /product="ATP-dependent RNA helicase DDX50 isoform 1"
                     /protein_id="NP_444413.1"
                     /db_xref="CCDS:CCDS23892.1"
                     /db_xref="GeneID:94213"
                     /db_xref="MGI:MGI:2182303"
                     /translation="MPGKLLWGDIMELEAPLEESESQRKERQKSDRRKSRHHSESEER
                     TETRENGVTDDLDAPKPKKAKMREKLNGDTKEGLRFSDEFSPSHKSRRKDLPNGDVDE
                     YEKRSKRVSSSENSHKSSDKAEETLTREQKEGAFSNFSISEETIKLLKGRGVTYLFPI
                     QVKTFGPVYEGKDLIAQARTGTGKTFSFAIPLIERLQRNQETIKKSRSPKVLVLAPTR
                     ELANQVAKDFKDITRKLSVACFYGGTSYQSQINQIRNGIDILVGTPGRIKDHLQSGRL
                     DLSKLRHVVLDEVDQMLDLGFAEQVEDIIHESYKTDSEDNPQTLLFSATCPQWVYKVA
                     KKYMKSRYEQVDLVGKMTQKAATTVEHLAIQCHWSQRPAVIGDVLQVYSGSEGRAIIF
                     CETKKNVTEMAMNPHIKQNAQCLHGDIAQSQREITLKGFREGSFKVLVATNVAARGLD
                     IPEVDLVIQSSPPQDVESYIHRSGRTGRAGRTGICVCFYQPRERGQLRYVEQKAGITF
                     KRVGVPSTMDLVKSKSMDAIRSLASVSYAAVDFFRPSAQRLIEEKGAVDALAAALAHI
                     SGASSFEPRSLITSDKGFVTMTLESPEEIQDVSCAWKELNRKLSSNAVSHVTRMCLLK
                     GNMGVCFDVPTSESERLQAEWHDSDWILSVPAKLPEIEEYYDGNTSSNPRQRSGWSGG
                     RSGRSGRSGGRSGGRSGRQSRQGSRSGSRQDGRRRSGNRNRSRSGGHKRNFD"
     exon            195..482
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            483..558
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            559..737
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            738..855
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            856..1041
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1042..1187
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1337
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1499
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1619
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1693
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1853
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1854..1988
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            1989..2033
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     exon            2034..2758
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2503..2508
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /note="hexamer: ATTAAA"
     polyA_site      2535
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /note="major polyA site"
     polyA_site      2599
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
     regulatory      2620..2625
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /note="hexamer: TATAAA"
     regulatory      2635..2640
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /note="hexamer: ATTACA"
     polyA_site      2643
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
     polyA_site      2656
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
     regulatory      2730..2735
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
                     /note="hexamer: AATAGA"
     polyA_site      2747
                     /gene="Ddx50"
                     /gene_synonym="4933429B04Rik; 8430408E17Rik; GU2; RH-II"
ORIGIN      
        1 acggtcctgg ccggcggggc cgcccagccc ccgcttcctt tcacgctctc ggtggccgta
       61 ggtggtcgcg gccaccgcag ccgggcggga ggttgcgacg gccagtaatg cccgggaaac
      121 tcctctgggg agacataatg gagctcgagg cacccttgga agagtccgag agccagagga
      181 aggagcggca aaagagtgat agaaggaagt caaggcacca ttctgaatca gaggagagaa
      241 cagaaacaag agaaaatggt gttacagatg acctggatgc tcccaagccc aaaaaagcta
      301 aaatgagaga aaagctaaat ggagacacca aagaaggact tagattttca gatgaattct
      361 ccccatctca taagtcaaga agaaaagacc tgccaaatgg agatgtggat gagtatgaga
      421 aaaggtcaaa gcgagtgtca tcctcagaaa actctcacaa gtcaagcgat aaagcagagg
      481 agactctaac acgtgaacaa aaggaaggag ccttctctaa tttctccatt tctgaggaga
      541 ccataaagct tctgaaaggt cgaggggtaa catatctctt tcctattcaa gttaaaacct
      601 ttggtcctgt atatgaagga aaagatttaa ttgctcaagc ccggacagga acaggaaaga
      661 cattctcttt tgccataccc ttgattgaaa ggctccaaag aaatcaagaa acaattaaaa
      721 aaagccgctc accaaaggta ctcgttttgg ctccaacaag ggaattggca aatcaagtag
      781 ccaaagattt caaagatata actagaaaac tcagtgtggc gtgtttttat ggtggaacat
      841 catatcaaag ccagattaat caaattcgga atggtattga catccttgtt gggaccccag
      901 ggcgtatcaa agaccatctg caaagtggcc gtttggatct ttcaaagcta cgccatgttg
      961 tgcttgatga agtagatcaa atgttagatt taggttttgc tgaacaagtc gaagatatta
     1021 ttcatgaatc ttacaaaact gattcggaag acaatcctca gaccttactt ttttctgcaa
     1081 cttgcccaca gtgggtatac aaagttgcaa aaaaatacat gaaatccaga tacgaacagg
     1141 ttgaccttgt tggaaaaatg actcaaaaag ctgcaaccac tgtggaacat ttggctatcc
     1201 agtgccattg gtctcagaga ccagcagtta ttggagatgt ccttcaggtc tacagtgggt
     1261 ctgaagggag agctattatc ttctgtgaaa ctaagaagaa tgttactgaa atggccatga
     1321 atccacacat aaagcagaat gctcagtgtt tgcatggaga cattgcacag tcacaaagag
     1381 aaattacctt gaaaggcttc agagaaggta gttttaaagt tttggtagca accaatgtgg
     1441 ctgctcgtgg cttggacatt cctgaggttg acctagtgat tcagagttct cctccacagg
     1501 atgttgaatc ctatatccat cgctctggac gcacaggtag agctggccgg acaggaattt
     1561 gtgtatgttt ttatcaacca agggaaagag gtcaactgag atatgtggag cagaaagctg
     1621 gaattacttt taaacgtgta ggtgttccgt ctacaatgga tttagttaaa tctaaaagca
     1681 tggatgccat caggtctctg gcttctgttt cttatgctgc tgttgatttt ttccgaccat
     1741 cagctcaaag actgatagaa gagaaggggg cagtggatgc attggctgca gcattagctc
     1801 acatttctgg tgcatcaagc tttgaaccac gatctttgat aacttctgat aaggggtttg
     1861 tgaccatgac tctggagagc ccagaagaaa tacaggatgt cagctgcgct tggaaggaac
     1921 ttaacagaaa gctcagtagt aatgctgtgt ctcacgtaac cagaatgtgc ctcctgaaag
     1981 gaaatatggg tgtttgcttt gatgttccta caagtgagtc agaaagatta caggcagagt
     2041 ggcatgattc agactggata ctctcagtgc cagccaaatt acctgaaatt gaagaatatt
     2101 atgatggaaa cacatcatct aatcccagac agaggagcgg ctggtctggc ggcaggtcag
     2161 gccgttcagg acggtctggt ggtcgatctg gtggccgatc aggtagacag agtcgacagg
     2221 gaagtcggtc aggaagtcgg caagatggta gaagacgaag tgggaacaga aatcgatcaa
     2281 gaagtggggg ccataaacgg aattttgact gagtgtttga tagttaatct tccagtgtga
     2341 gcttgcctat ttctgtctaa tcatgtatat tattcaccaa aaattagatc atcatagttg
     2401 aggtatttgt ctgtaatttg caaagaagtt ggtcgtattt ttttaaaaag tatttcacaa
     2461 gaatggttct aaacaaatct acttatctgg ctataccttt gaattaaaaa aaagcccttt
     2521 tattgtctgt ttttattctg tgttaagagt tttctcaatg taatttttta aaatttctaa
     2581 gatgtgacag tcaagttaac cttaaggttt cctgtaatat ataaaatgtg gcatattaca
     2641 ccattctttc atgtgatgaa ggacaaaacc ctttctacat aagggctggt agctggtgac
     2701 ttgtttggtg aagattactg gtttaactta atagacatat tcaggtagat tggtgtgc
//
