LOCUS       NM_153065               2592 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus DEAD box helicase 27 (Ddx27), mRNA.
ACCESSION   NM_153065
VERSION     NM_153065.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Chai,G., Webb,A., Li,C., Antaki,D., Lee,S., Breuss,M.W., Lang,N.,
            Stanley,V., Anzenberg,P., Yang,X., Marshall,T., Gaffney,P.,
            Wierenga,K.J., Chung,B.H., Tsang,M.H., Pais,L.S., Lovgren,A.K.,
            VanNoy,G.E., Rehm,H.L., Mirzaa,G., Leon,E., Diaz,J., Neumann,A.,
            Kalverda,A.P., Manfield,I.W., Parry,D.A., Logan,C.V., Johnson,C.A.,
            Bonthron,D.T., Valleley,E.M.A., Issa,M.Y., Abdel-Ghafar,S.F.,
            Abdel-Hamid,M.S., Jennings,P., Zaki,M.S., Sheridan,E. and
            Gleeson,J.G.
  TITLE     Mutations in Spliceosomal Genes PPIL1 and PRP17 Cause
            Neurodegenerative Pontocerebellar Hypoplasia with Microcephaly
  JOURNAL   Neuron 109 (2), 241-256 (2021)
   PUBMED   33220177
REFERENCE   3  (bases 1 to 2592)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2592)
  AUTHORS   Fisher,C.L., Marks,H., Cho,L.T., Andrews,R., Wormald,S.,
            Carroll,T., Iyer,V., Tate,P., Rosen,B., Stunnenberg,H.G.,
            Fisher,A.G. and Skarnes,W.C.
  TITLE     An efficient method for generation of bi-allelic null mutant mouse
            embryonic stem cells and its application for investigating
            epigenetic modifiers
  JOURNAL   Nucleic Acids Res 45 (21), e174 (2017)
   PUBMED   28981838
REFERENCE   5  (bases 1 to 2592)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2592)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK134274.1.
            
            On Apr 6, 2007 this sequence version replaced NM_153065.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134274.1, AK146469.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2592              AK134274.1         2-2593
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 87.22 cM"
     gene            1..2592
                     /gene="Ddx27"
                     /note="DEAD box helicase 27"
                     /db_xref="GeneID:228889"
                     /db_xref="MGI:MGI:2385884"
     exon            1..114
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..2304
                     /gene="Ddx27"
                     /EC_number="3.6.4.13"
                     /note="DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27;
                     RNA-helicase like protein; DEAD box protein 27; DEAD
                     (Asp-Glu-Ala-Asp) box polypeptide 27"
                     /codon_start=1
                     /product="probable ATP-dependent RNA helicase DDX27"
                     /protein_id="NP_694705.2"
                     /db_xref="CCDS:CCDS38336.1"
                     /db_xref="GeneID:228889"
                     /db_xref="MGI:MGI:2385884"
                     /translation="MLAELGFIRTIGENDEVPVEPESDSGDEEEEGPIVLGRKQKALQ
                     KNRSADFNPDFVFTEKEGMYDGSWALADVMSQLKKKRAATTLDEKIEKVRKRRKAEDK
                     EAKSGKVEEKEGQADSDLKGQENPGEDEAGSKDEDSETDYSSEDEEILTKADTLKVKE
                     KKKKKKGQAAGGFFEDASEYDKSLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVG
                     LLGKDICACAATGTGKTAAFALPVLERLIYKPRQAAVTRVLVLVPTRELGIQVHSVTK
                     QLAQFCSITTCLAVGGLDVKSQEAALRAAPDILIATPGRLIDHLHNCPSFHLSSIEVL
                     ILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNPVRIFVN
                     SNTDVAPFLRQEFIRIRPNREGDREAIVAALLMRTFTDHVMLFTQTKKQAHRMHILLG
                     LLGLQVGELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPN
                     TVKHYVHRVGRTARAGRAGRSVSLVGEEERKMLKEIVKAAKAPVKARILPQDVILKFR
                     DKIEKLEKDVYAVLQLEAEEKEMQQSEAQIDTAQRLLAKGKETADQEPERSWFQTKEE
                     RKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERSQFEILKAQMFAERLAK
                     RNRRTKRARAMPEDEPTGPAKKQKQQQKSVFDEELTNTSKKALKQYRAGPSFEERKQS
                     GLPRQRRGNFKSKSRYKRKK"
     exon            115..261
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            262..321
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            322..481
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            482..525
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            526..612
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            613..718
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            719..892
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            893..1043
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1143
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1285
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            1286..1439
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1521
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1699
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1806
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1909
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2004
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            2005..2125
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            2126..2213
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..2285
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     exon            2286..2592
                     /gene="Ddx27"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2561..2566
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddx27"
                     /note="hexamer: AATAAA"
     polyA_site      2582
                     /gene="Ddx27"
                     /note="major polyA site"
ORIGIN      
        1 ggaagtggct gcttcttcag catgctggcg gagctcggct tcatccggac catcggcgag
       61 aatgacgagg tgccggttga gcccgagtcg gactccggag acgaggagga ggagggaccc
      121 attgtgctgg gccgcaagca gaaagccttg cagaagaacc gcagtgctga cttcaaccct
      181 gactttgttt tcactgagaa ggagggcatg tatgatggca gctgggccct ggctgatgtc
      241 atgagccagc tcaagaagaa gcgggcagcc actacactag atgagaagat tgagaaagtc
      301 cggaagagaa ggaaggcaga ggacaaggaa gccaagtctg ggaaggtgga ggagaaagaa
      361 ggccaggcag actctgacct gaaggggcag gagaatccgg gtgaggatga ggcaggctcc
      421 aaggatgaag actctgagac tgactactcc tctgaggatg aggaaatcct caccaaagca
      481 gacacactca aagtgaagga gaagaagaag aagaagaagg gccaggcagc aggaggattc
      541 tttgaagatg catccgagta tgacaaaagc ctgtccttcc aggacatgaa cctctcccgg
      601 cccctcctga aggccattac agccatgggc ttcaagcagc ccacgccaat ccagaaggcg
      661 tgtatccctg tgggtctgct ggggaaggac atctgcgcct gtgcagccac tgggacaggc
      721 aaaactgcag cctttgcact gcccgtcttg gagcgtctga tctacaagcc ccgccaggct
      781 gcagtcactc gcgtgctggt gctggtcccc acgagagagc tgggcatcca agtgcactct
      841 gtcaccaagc agctggccca gttctgcagc atcactacct gcctggctgt gggtggcctg
      901 gatgtgaagt ctcaggaagc cgctcttcgg gcagcaccag acatcctcat tgccacacct
      961 ggccggctca tcgaccacct ccataactgc ccctccttcc acctgagcag cattgaagtg
     1021 ctcatcttgg atgaggctga caggatgctg gatgagtatt tcgaggagca gatgaaggag
     1081 attatccgaa tgtgttccca ccaccgccag accatgctct tctcggccac catgacagat
     1141 gaggtgaaag atctggcttc ggtctcctta aagaatcctg tgcggatatt tgtgaacagc
     1201 aacacagatg tcgctccctt cctgcggcaa gagttcatac ggatccggcc taatagggaa
     1261 ggggaccggg aggccattgt ggcagctctg ctgatgagaa ccttcactga ccacgtgatg
     1321 ctcttcaccc agaccaagaa gcaggcccat cgcatgcaca tcctcctggg gctgctgggg
     1381 ctgcaggtgg gcgagctcca cggcaacttg tcacagaccc agcgcctgga ggccctccgg
     1441 cgctttaagg acgaacagat tgacatcctg gtggccacag atgtggcagc ccgcggtctt
     1501 gacattgaag gagtgaaaac agtgatcaac ttcaccatgc ccaacactgt taaacactat
     1561 gtgcaccggg tggggcggac ggcccgtgct ggcagggctg gacgctctgt ctccctagtg
     1621 ggagaggagg agcggaagat gctgaaggag atcgtgaagg ctgccaaggc gccagtgaag
     1681 gctcggatcc ttccccagga tgttatcctc aagttccggg acaagattga gaaattggag
     1741 aaagatgtgt atgcagtgct gcagctggag gcggaggaaa aagagatgca gcagtcggaa
     1801 gcccagattg acacagcaca gcgactctta gccaaaggga aagagacagc agaccaggag
     1861 cctgagagga gctggttcca gaccaaagaa gagaggaaga aggagaagat tgctaaggct
     1921 ctacaggagt ttgacttggc cttaagagga aagaagaaaa ggaagaagtt tatgaaggat
     1981 gccaagaaaa agggggagat gacggcagag gagaggtccc agtttgaaat cctcaaggca
     2041 cagatgtttg cagagcggct ggccaagagg aatcgcagaa ccaagcgagc acgggcaatg
     2101 cctgaggacg agcctacagg ccctgctaaa aagcagaagc agcagcagaa gtctgtgttt
     2161 gatgaagaac tcaccaacac aagcaagaag gccctgaaac agtatcgggc tggcccttcc
     2221 tttgaagaga ggaaacagtc aggtctgccc cgccagagga gagggaactt caaatctaag
     2281 tccaggtaca agagaaagaa gtagtctcag tggtgtgaag aagttggtgg gacaatttta
     2341 tatcccttcc ctgtgggaag tcatcttgtc ttggtctggt ttttttccat atataaaacc
     2401 ccaaacctgg ggctggagag atggctcaga ctgctcttcc agaggtcctg agttcaattc
     2461 tcagcgacca catggtggct tataaccatc tataatggga tccgatgccc tcttctaatg
     2521 ggtctgaaga cagctacagt gtactcacat acataaaata aataaaatct ttaagaacag
     2581 cagaccttct tg
//
