LOCUS       NM_013932               1675 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus DEAD box helicase 25 (Ddx25), mRNA.
ACCESSION   NM_013932 XM_983938
VERSION     NM_013932.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Anbazhagan,R., Kavarthapu,R., Dale,R., Campbell,K., Faucz,F.R. and
            Dufau,M.L.
  TITLE     miRNA Expression Profiles of Mouse Round Spermatids in
            GRTH/DDX25-Mediated Spermiogenesis: mRNA-miRNA Network Analysis
  JOURNAL   Cells 12 (5), 756 (2023)
   PUBMED   36899892
  REMARK    GeneRIF: miRNA Expression Profiles of Mouse Round Spermatids in
            GRTH/DDX25-Mediated Spermiogenesis: mRNA-miRNA Network Analysis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Kavarthapu,R., Anbazhagan,R., Pal,S. and Dufau,M.L.
  TITLE     Single-Cell Transcriptomic Profiling of the Mouse Testicular Germ
            Cells Reveals Important Role of Phosphorylated GRTH/DDX25 in Round
            Spermatid Differentiation and Acrosome Biogenesis during
            Spermiogenesis
  JOURNAL   Int J Mol Sci 24 (4), 3127 (2023)
   PUBMED   36834539
  REMARK    GeneRIF: Single-Cell Transcriptomic Profiling of the Mouse
            Testicular Germ Cells Reveals Important Role of Phosphorylated
            GRTH/DDX25 in Round Spermatid Differentiation and Acrosome
            Biogenesis during Spermiogenesis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1675)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1675)
  AUTHORS   Anbazhagan,R., Kavarthapu,R., Coon,S.L. and Dufau,M.L.
  TITLE     Role of Phosphorylated Gonadotropin-Regulated Testicular RNA
            Helicase (GRTH/DDX25) in the Regulation of Germ Cell Specific mRNAs
            in Chromatoid Bodies During Spermatogenesis
  JOURNAL   Front Cell Dev Biol 8, 580019 (2020)
   PUBMED   33425888
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1675)
  AUTHORS   Snyder,E., Chukrallah,L., Seltzer,K., Goodwin,L. and Braun,R.E.
  TITLE     ADAD1 and ADAD2, testis-specific adenosine deaminase
            domain-containing proteins, are required for male fertility
  JOURNAL   Sci Rep 10 (1), 11536 (2020)
   PUBMED   32665638
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1675)
  AUTHORS   Sato,H., Tsai-Morris,C.H. and Dufau,M.L.
  TITLE     Relevance of gonadotropin-regulated testicular RNA helicase
            (GRTH/DDX25) in the structural integrity of the chromatoid body
            during spermatogenesis
  JOURNAL   Biochim Biophys Acta 1803 (5), 534-543 (2010)
   PUBMED   20176059
REFERENCE   7  (bases 1 to 1675)
  AUTHORS   Gutti,R.K., Tsai-Morris,C.H. and Dufau,M.L.
  TITLE     Gonadotropin-regulated testicular helicase (DDX25), an essential
            regulator of spermatogenesis, prevents testicular germ cell
            apoptosis
  JOURNAL   J Biol Chem 283 (25), 17055-17064 (2008)
   PUBMED   18430733
  REMARK    GeneRIF: GRTH, as a component of mRNP particles, acts as a negative
            regulator of the tumor necrosis factor receptor 1 and caspase
            pathways and promotes NF-kappaB function to control apoptosis in
            spermatocytes of adult mice
REFERENCE   8  (bases 1 to 1675)
  AUTHORS   Sheng,Y., Tsai-Morris,C.H., Gutti,R., Maeda,Y. and Dufau,M.L.
  TITLE     Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a
            transport protein involved in gene-specific mRNA export and protein
            translation during spermatogenesis
  JOURNAL   J Biol Chem 281 (46), 35048-35056 (2006)
   PUBMED   16968703
REFERENCE   9  (bases 1 to 1675)
  AUTHORS   Tsai-Morris,C.H., Sheng,Y., Lee,E., Lei,K.J. and Dufau,M.L.
  TITLE     Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is
            essential for spermatid development and completion of
            spermatogenesis
  JOURNAL   Proc Natl Acad Sci U S A 101 (17), 6373-6378 (2004)
   PUBMED   15096601
  REMARK    GeneRIF: demonstrates that GRTH is essential for completion of
            spermatogenesis, (ii) provides insights into intrinsic requirements
            for spermiogenesis, and (iii) establishes a model for studies of
            male infertility and contraception
REFERENCE   10 (bases 1 to 1675)
  AUTHORS   Tang,P.Z., Tsai-Morris,C.H. and Dufau,M.L.
  TITLE     A novel gonadotropin-regulated testicular RNA helicase. A new
            member of the dead-box family
  JOURNAL   J Biol Chem 274 (53), 37932-37940 (1999)
   PUBMED   10608860
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CF104207.1 and AK078340.1.
            
            On Dec 22, 2007 this sequence version replaced NM_013932.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK078340.1, AK050693.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                CF104207.1         1-42
            43-1675             AK078340.1         2-1634
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 20.31 cM"
     gene            1..1675
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /note="DEAD box helicase 25"
                     /db_xref="GeneID:30959"
                     /db_xref="MGI:MGI:1353582"
     exon            1..133
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..1525
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /EC_number="3.6.4.13"
                     /note="DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 25;
                     DEAD box protein 25; gonadotropin-regulated testicular RNA
                     helicase; DEAD (Asp-Glu-Ala-Asp) box polypeptide 25; DEAD
                     (aspartate-glutamate-alanine-aspartate) box polypeptide
                     25"
                     /codon_start=1
                     /product="ATP-dependent RNA helicase DDX25"
                     /protein_id="NP_038960.2"
                     /db_xref="CCDS:CCDS52753.1"
                     /db_xref="GeneID:30959"
                     /db_xref="MGI:MGI:1353582"
                     /translation="MASLLWGGDAGAAESERLNSHFSNLVHPRKNLRGIRSTTVPNID
                     GSLNTEDDDDDEDDVVDLAANSLLNKLIRQSLIESSHRVEVLQKDPSSPLYSVKTFEE
                     LRLKEELLKGIYAMGFNRPSKIQEMALPMMLAHPPQNLIAQSQSGTGKTAAFVLAMLS
                     RVNALELFPQCLCLAPTYELALQTGRVVERMGKFCVDVEVMYAIRGNRIPRGTEVTKQ
                     IIIGTPGTVLDWCFKRKLIDLTKIRVFVLDEADVMIDTQGFSDQSIRIQRALPSECQM
                     LLFSATFEDSVWQFAERIIPDPNVIKLRKEELTLNNIRQYYVLCENRKGKYQALCNIY
                     GGITIGQAIIFCQTRRNAKWLTVEMMQDGHQVSLLSGELTVEQRASIIQRFRDGKEKV
                     LITTNVCARGIDVKQVTIVVNFDLPVNQSEEPDYETYLHRIGRTGRFGKKGLAFNMIE
                     VDKLPLLMKIQDHFNSNIKQLDPEDMDEIEKIEY"
     exon            134..200
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            201..248
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            249..384
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            385..477
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            478..580
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            581..695
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            696..873
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            874..1111
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1112..1274
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1463
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1675
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1649..1654
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /note="putative"
     polyA_site      1675
                     /gene="Ddx25"
                     /gene_synonym="GRTH"
                     /note="putative"
ORIGIN      
        1 tgagccgagc cggcgctgcc ccgcgcgggg gtggcagcac gtgccagggg cgagagcgga
       61 gaccgcagct atggcgtcgt tactttgggg aggcgacgcc ggggcggcgg agagcgagcg
      121 gctgaacagc cacttttcaa atctcgtcca tcccaggaag aaccttcgtg gtattaggag
      181 taccacagtc ccaaacatag acggctctct taacacggaa gatgatgatg atgatgaaga
      241 cgatgtagtg gatttggcag ccaattcact cttaaacaag ctaatccgtc agtccttaat
      301 agaatccagc caccgagtgg aagtcttaca gaaggatccc agctctccac tctactcggt
      361 gaaaaccttt gaagagctac ggctaaaaga agaattacta aaagggattt atgcaatggg
      421 atttaaccga ccatccaaaa tccaagagat ggccctcccc atgatgctag cacaccctcc
      481 ccagaacctc atagcacaga gccagtccgg cacaggaaaa actgctgcct ttgtcttagc
      541 aatgctaagc cgagtgaatg ccttggaact gtttccacag tgcctctgcc tggctcctac
      601 ctatgaactg gctctgcaga ccggtcgtgt ggttgaaagg atgggcaagt tctgcgtgga
      661 tgttgaagtg atgtatgcca ttcgaggaaa ccgaattcca aggggcaccg aagtcaccaa
      721 acagattatt attggtactc ctgggactgt cctagattgg tgtttcaagc gaaaactgat
      781 tgatttgaca aagatccgtg tgtttgtcct ggatgaagca gatgtgatga ttgacaccca
      841 aggattctcc gatcagagca tccgtatcca aagagcttta ccctctgaat gccagatgct
      901 cctcttttcg gcaacctttg aggactctgt gtggcagttt gctgaacgaa tcattcctga
      961 ccccaatgtt atcaagttgc gaaaagagga actgaccctg aacaacatcc ggcagtatta
     1021 cgtgttgtgt gagaacagga aaggcaaata ccaagccctc tgtaacatct acggtggcat
     1081 caccattggc caagccatta tcttctgcca gacacgtcga aatgccaagt ggctgactgt
     1141 ggagatgatg caggatggcc accaggtgtc cctgctaagt ggggaactca cagtggagca
     1201 gcgagcttcc atcattcaga gattccgaga tggcaaagag aaggttctca taacgaccaa
     1261 tgtctgtgct cgagggattg atgtgaagca ggtcaccatc gtcgtgaact ttgacctccc
     1321 tgtaaaccaa tcagaagagc cagactatga gacctacctc caccgtatag ggcggacggg
     1381 acgctttggg aaaaaaggtc tcgccttcaa catgattgaa gtggataagc tgcccctgct
     1441 tatgaaaata caagaccact tcaatagcaa tattaagcag cttgacccag aagacatgga
     1501 tgagattgag aagatcgaat attgaagaga gagagagaag agaggccttc gttgtttgtg
     1561 ggctagttta cgtgagaatg tttcagcaga gtttgaagat aattttctat gttgagactc
     1621 tgtcaaggtg aaactgtcac agataaaaaa taaatgccat gactctcttc acccg
//
