LOCUS       NM_007838               2120 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus dolichyl-di-phosphooligosaccharide-protein
            glycotransferase (Ddost), mRNA.
ACCESSION   NM_007838
VERSION     NM_007838.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Dong,J., Wei,Z. and Zhu,Z.
  TITLE     LncRNA TSIX aggravates spinal cord injury by regulating the
            PI3K/AKT pathway via the miR-532-3p/DDOST axis
  JOURNAL   J Biochem Mol Toxicol 37 (8), e23384 (2023)
   PUBMED   37155292
  REMARK    GeneRIF: LncRNA TSIX aggravates spinal cord injury by regulating
            the PI3K/AKT pathway via the miR-532-3p/DDOST axis.
REFERENCE   3  (bases 1 to 2120)
  AUTHORS   Ramirez,A.S., Kowal,J. and Locher,K.P.
  TITLE     Cryo-electron microscopy structures of human
            oligosaccharyltransferase complexes OST-A and OST-B
  JOURNAL   Science 366 (6471), 1372-1375 (2019)
   PUBMED   31831667
REFERENCE   4  (bases 1 to 2120)
  AUTHORS   Zhuang,A., Yap,F.Y.T., McCarthy,D., Leung,C., Sourris,K.C.,
            Penfold,S.A., Thallas-Bonke,V., Coughlan,M.T., Schulz,B.L. and
            Forbes,J.M.
  TITLE     Globally elevating the AGE clearance receptor, OST48, does not
            protect against the development of diabetic kidney disease, despite
            improving insulin secretion
  JOURNAL   Sci Rep 9 (1), 13664 (2019)
   PUBMED   31541173
  REMARK    GeneRIF: Globally elevating the AGE clearance receptor, OST48, does
            not protect against the development of diabetic kidney disease,
            despite improving insulin secretion.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2120)
  AUTHORS   Zhuang,A., Yap,F.Y., Bruce,C., Leung,C., Plan,M.R., Sullivan,M.A.,
            Herath,C., McCarthy,D., Sourris,K.C., Kantharidis,P.,
            Coughlan,M.T., Febbraio,M.A., Hodson,M.P., Watt,M.J., Angus,P.,
            Schulz,B.L. and Forbes,J.M.
  TITLE     Increased liver AGEs induce hepatic injury mediated through an
            OST48 pathway
  JOURNAL   Sci Rep 7 (1), 12292 (2017)
   PUBMED   28947796
  REMARK    GeneRIF: Study demonstrated that
            dolichyl-diphosphooligosaccharide-protein glycosyltransferase Ost48
            (encoded by Ddost gene) is a facilitator of increased advanced
            glycation end-products (AGE) deposition in the liver and OST48-AGE
            pathway leads to the onset of liver injury in combination with
            central adiposity and glucose intolerance, despite ample physical
            activity.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2120)
  AUTHORS   Kucej,M., Fermaintt,C.S., Yang,K., Irizarry-Caro,R.A. and Yan,N.
  TITLE     Mitotic Phosphorylation of TREX1 C Terminus Disrupts TREX1
            Regulation of the Oligosaccharyltransferase Complex
  JOURNAL   Cell Rep 18 (11), 2600-2607 (2017)
   PUBMED   28297665
  REMARK    GeneRIF: the cell-cycle-dependent post-translation modification of
            TREX1 regulates its interaction with OST.
REFERENCE   7  (bases 1 to 2120)
  AUTHORS   Hasan,M., Fermaintt,C.S., Gao,N., Sakai,T., Miyazaki,T., Jiang,S.,
            Li,Q.Z., Atkinson,J.P., Morse,H.C. 3rd, Lehrman,M.A. and Yan,N.
  TITLE     Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase
            Activity Independent of Nuclease Activity to Suppress Immune
            Activation
  JOURNAL   Immunity 43 (3), 463-474 (2015)
   PUBMED   26320659
  REMARK    GeneRIF: Data show that oligosaccharyltransferase (OST) activity is
            dysregulated in three prime exonuclease 1 knockout (Trex1-/-)
            cells.
REFERENCE   8  (bases 1 to 2120)
  AUTHORS   Cai,W., Torreggiani,M., Zhu,L., Chen,X., He,J.C., Striker,G.E. and
            Vlassara,H.
  TITLE     AGER1 regulates endothelial cell NADPH oxidase-dependent oxidant
            stress via PKC-delta: implications for vascular disease
  JOURNAL   Am J Physiol Cell Physiol 298 (3), C624-C634 (2010)
   PUBMED   19955485
  REMARK    GeneRIF: AGER1 provides protection against AGE-induced reactive
            oxygen species generation via NADPH oxidase and protein kinase C
            delta.
REFERENCE   9  (bases 1 to 2120)
  AUTHORS   Yamagata,T., Tsuru,T., Momoi,M.Y., Suwa,K., Nozaki,Y., Mukasa,T.,
            Ohashi,H., Fukushima,Y. and Momoi,T.
  TITLE     Genome organization of human 48-kDa oligosaccharyltransferase
            (DDOST)
  JOURNAL   Genomics 45 (3), 535-540 (1997)
   PUBMED   9367678
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC068132.1 and BU755176.1.
            
            On Apr 27, 2018 this sequence version replaced NM_007838.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068132.1, AK084267.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2095              BC068132.1         1-2095
            2096-2120           BU755176.1         1-25                c
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 70.13 cM"
     gene            1..2120
                     /gene="Ddost"
                     /note="dolichyl-di-phosphooligosaccharide-protein
                     glycotransferase"
                     /db_xref="GeneID:13200"
                     /db_xref="MGI:MGI:1194508"
     exon            1..301
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..1467
                     /gene="Ddost"
                     /EC_number="2.4.99.18"
                     /note="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase 48 kDa subunit; DDOST 48 kDa subunit;
                     oligosaccharyl transferase 48 kDa subunit"
                     /codon_start=1
                     /product="dolichyl-diphosphooligosaccharide--protein
                     glycosyltransferase 48 kDa subunit precursor"
                     /protein_id="NP_031864.2"
                     /db_xref="CCDS:CCDS18824.1"
                     /db_xref="GeneID:13200"
                     /db_xref="MGI:MGI:1194508"
                     /translation="MKMDPRLAVRAWPLCGLLLAVLGCVCASGPRTLVLLDNLNVRDT
                     HSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETIS
                     AFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSDLGQHTLIVA
                     DTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQY
                     PHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQTGNYEL
                     AVALSRWVFKEEGVLRVGPVSHHRVGEMAPPNAYTVTDLVEYSIIIEQLSNGKWVPFD
                     GDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQ
                     VSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMKEKEKSD"
     exon            302..412
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            413..499
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            500..603
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            604..698
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            699..792
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            793..941
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1089
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1210
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1317
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..2103
                     /gene="Ddost"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1688..1693
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddost"
                     /note="hexamer: AATAAA"
     polyA_site      1717
                     /gene="Ddost"
                     /note="major polyA site"
     regulatory      2070..2075
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddost"
                     /note="hexamer: AATAAA"
     polyA_site      2103
                     /gene="Ddost"
                     /note="major polyA site"
ORIGIN      
        1 gtccgtcccc gcagcggcgt gggccgcggg gccacggagt acccggaccg cttccaagtg
       61 gtactccgcc gctgcgagcg gccattgggc ggccgcgagg gacgcttccg gtgcccaggt
      121 gctggcctgg gctgctggga gatgaagatg gatccccgcc tcgccgtccg cgcctggccc
      181 ctctgcgggc tgctgctggc cgtgctcggc tgcgtctgcg ccagcggccc ccgcaccctc
      241 gtgctgctgg acaacctgaa cgtgcgggac acgcactcgc tgttcttccg cagcctgaag
      301 gaccggggct ttgagctcac cttcaagacc gcagatgacc ccagcttgtc cctcattaag
      361 tacggggagt tcctctatga caaccttatc atcttttccc cgtcggtgga agattttgga
      421 ggcaacatca acgtggagac catcagtgcc ttcattgatg gtggcggcag cgttttggtg
      481 gctgccagct ctgatattgg tgaccctctt cgggagctgg gcagtgagtg tgggattgaa
      541 tttgatgaag agaaaacagc tgtcatcgac caccacaact atgatgtttc tgaccttggc
      601 cagcacacac tcattgtggc tgacactgag aacctgctga aggccccgac cattgttggc
      661 aagtcatctc tgaaccccat tctcttccga ggagttggaa tggtggcaga cccggacaat
      721 cccttggttt tggacatcct aacaggctct tcaacctctt actccttctt cccagataaa
      781 ccaatcaccc agtaccccca tgcggtgggg aggaacactc tgctgattgc cgggctccag
      841 gccaggaaca acgcccgggt catcttcagt ggctctctgg atttcttcag cgatgccttc
      901 ttcaactcgg cagtgcagaa ggccacaccc ggtgcgcaga ggtattctca gacaggcaac
      961 tatgaactag ctgtggccct ctcacgctgg gtgttcaagg aggagggtgt ccttcgagta
     1021 gggcctgtgt cccatcaccg ggtgggcgag atggctccac ccaatgccta cactgtcacc
     1081 gacttggtgg agtatagcat catcatagaa cagctctcca atggcaagtg ggtccccttt
     1141 gatggtgatg acattcagct ggagttcgtg cgcatcgacc ccttcgtgag gaccttcctg
     1201 aagaggaaag gtggcaagta cagtgtccag ttcaagctgc ctgacgtgta tggtgtattc
     1261 cagtttaaag tggattacaa ccggctaggc tacacccacc tgtactcttc cacccaggtg
     1321 tcagtgaggc cactccagca cacgcagtat gagcgcttca tcccctcggc ctatccctac
     1381 tatgccagtg ccttctccat gatggccggg ctcttcatct tcagcatcgt cttcttgcac
     1441 atgaaggaga aggagaagtc tgactgagcc agaacctggg gtccacacag tacggccgag
     1501 gccatcacga atggatcggt cgggttctgt tttgttttgt aaaggccatg ggaaaatggc
     1561 acagccttac ctcagagggc gaggactatg gggtgggggg cagggaacat tttttttttt
     1621 ctgtaagttt tccaagtttg aatcactctg tggcaatttt tgtatgtgca gccgccccca
     1681 tttctaaaat aaagagaacc actgccctca cctgtcacct ggccccatga cactgatgat
     1741 ggcagggctg ctgcctaggg acactgtgcc agagcctccg tgcctctcat ctctcctcat
     1801 ggaggtgggg atcacacacc cacagtgtag tggaaactag actgacagct ggcctaagaa
     1861 ttgctgctga agagacccga gagggactgg aaagatgact aggttaagag cactgcagag
     1921 gacccaggtt caagtcccag cacccacatg gcagctcaca accttctata atggtagtct
     1981 gaggagatct gatgcccttt tgtggcctcc caggagacca ggtatttaag tgatacatcg
     2041 tatgtacaga caaaacacga tatgcataaa ataaagaaaa cccctgtgtt cctgttcact
     2101 ggcaaaaaaa aaaaaaaaaa
//
