LOCUS       NM_028102               4266 bp    mRNA    linear   ROD 08-DEC-2024
DEFINITION  Mus musculus DDHD domain containing 2 (Ddhd2), transcript variant
            1, mRNA.
ACCESSION   NM_028102
VERSION     NM_028102.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Su,X., Liang,F., Zeng,Y., Yang,Z.R., Deng,Y.Z., Xu,Y.H. and
            Cai,X.W.
  TITLE     Radiation-Induced Endothelial Ferroptosis Accelerates
            Atherosclerosis via the DDHD2-Mediated Nrf2/GPX4 Pathway
  JOURNAL   Biomolecules 14 (7), 879 (2024)
   PUBMED   39062593
  REMARK    GeneRIF: Radiation-Induced Endothelial Ferroptosis Accelerates
            Atherosclerosis via the DDHD2-Mediated Nrf2/GPX4 Pathway.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Akefe,I.O., Saber,S.H., Matthews,B., Venkatesh,B.G., Gormal,R.S.,
            Blackmore,D.G., Alexander,S., Sieriecki,E., Gambin,Y.,
            Bertran-Gonzalez,J., Vitale,N., Humeau,Y., Gaudin,A., Ellis,S.A.,
            Michaels,A.A., Xue,M., Cravatt,B., Joensuu,M., Wallis,T.P. and
            Meunier,F.A.
  TITLE     The DDHD2-STXBP1 interaction mediates long-term memory via
            generation of saturated free fatty acids
  JOURNAL   EMBO J 43 (4), 533-567 (2024)
   PUBMED   38316990
  REMARK    GeneRIF: The DDHD2-STXBP1 interaction mediates long-term memory via
            generation of saturated free fatty acids.
REFERENCE   3  (bases 1 to 4266)
  AUTHORS   Maruyama,T., Baba,T., Maemoto,Y., Hara-Miyauchi,C.,
            Hasegawa-Ogawa,M., Okano,H.J., Enda,Y., Matsumoto,K., Arimitsu,N.,
            Nakao,K., Hamamoto,H., Sekimizu,K., Ohto-Nakanishi,T.,
            Nakanishi,H., Tokuyama,T., Yanagi,S., Tagaya,M. and Tani,K.
  TITLE     Loss of DDHD2, whose mutation causes spastic paraplegia, promotes
            reactive oxygen species generation and apoptosis
  JOURNAL   Cell Death Dis 9 (8), 797 (2018)
   PUBMED   30038238
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4266)
  AUTHORS   Inloes,J.M., Kiosses,W.B., Wang,H., Walther,T.C., Farese,R.V. Jr.
            and Cravatt,B.F.
  TITLE     Functional Contribution of the Spastic Paraplegia-Related
            Triglyceride Hydrolase DDHD2 to the Formation and Content of Lipid
            Droplets
  JOURNAL   Biochemistry 57 (5), 827-838 (2018)
   PUBMED   29278326
  REMARK    GeneRIF: Lipid droplets from brain tissue of DDHD2(-/-) mice
            contain both established LD-associated proteins identified
            previously in other organs and CNS-enriched proteins, including
            several proteins with genetic links to human neurological disease.
REFERENCE   5  (bases 1 to 4266)
  AUTHORS   Inloes,J.M., Hsu,K.L., Dix,M.M., Viader,A., Masuda,K., Takei,T.,
            Wood,M.R. and Cravatt,B.F.
  TITLE     The hereditary spastic paraplegia-related enzyme DDHD2 is a
            principal brain triglyceride lipase
  JOURNAL   Proc Natl Acad Sci U S A 111 (41), 14924-14929 (2014)
   PUBMED   25267624
  REMARK    GeneRIF: DDHD2(-/-) mice show age-dependent triacylglycerol
            elevations in the central nervous system, but not in several
            peripheral tissues.
REFERENCE   6  (bases 1 to 4266)
  AUTHORS   Baba,T., Kashiwagi,Y., Arimitsu,N., Kogure,T., Edo,A., Maruyama,T.,
            Nakao,K., Nakanishi,H., Kinoshita,M., Frohman,M.A., Yamamoto,A. and
            Tani,K.
  TITLE     Phosphatidic acid (PA)-preferring phospholipase A1 regulates
            mitochondrial dynamics
  JOURNAL   J Biol Chem 289 (16), 11497-11511 (2014)
   PUBMED   24599962
REFERENCE   7  (bases 1 to 4266)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   8  (bases 1 to 4266)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
REFERENCE   9  (bases 1 to 4266)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162367.5.
            
            On Jul 30, 2020 this sequence version replaced NM_028102.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC046229.1, SRR13861859.902450.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-94                AC162367.5         47704-47797
            95-320              AC162367.5         48810-49035
            321-511             AC162367.5         49693-49883
            512-601             AC162367.5         51191-51280
            602-722             AC162367.5         52136-52256
            723-812             AC162367.5         53356-53445
            813-948             AC162367.5         55030-55165
            949-1157            AC162367.5         60174-60382
            1158-1225           AC162367.5         60629-60696
            1226-1348           AC162367.5         62398-62520
            1349-1444           AC162367.5         63676-63771
            1445-1528           AC162367.5         65893-65976
            1529-1684           AC162367.5         66083-66238
            1685-1787           AC162367.5         66872-66974
            1788-1958           AC162367.5         67097-67267
            1959-2118           AC162367.5         68861-69020
            2119-2226           AC162367.5         74246-74353
            2227-4266           AC162367.5         74620-76659
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 14.17 cM"
     gene            1..4266
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /note="DDHD domain containing 2"
                     /db_xref="GeneID:72108"
                     /db_xref="MGI:MGI:1919358"
     exon            1..94
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            95..320
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..2200
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /EC_number="3.1.1.3"
                     /note="isoform 1 is encoded by transcript variant 1; SAM,
                     WWE and DDHD domain-containing protein 1; Phospholipase
                     DDHD2; DDHD domain-containing protein 2; TAG hydrolase;
                     triglyceride lipase; triglyceride hydrolase DDHD2;
                     triacylglycerol hydrolase DDHD2"
                     /codon_start=1
                     /product="triacylglycerol hydrolase DDHD2 isoform 1"
                     /protein_id="NP_082378.1"
                     /db_xref="CCDS:CCDS52529.1"
                     /db_xref="GeneID:72108"
                     /db_xref="MGI:MGI:1919358"
                     /translation="MSSGESHQEQLSQSDPSPSPNSCSSFELIDMDASSSYEPVSPHW
                     FYCKVLDSKELWIPFNSEDSQQLEDAYGSGKDCNERIVPTDGGRYDVHLGERMRYAVY
                     WDELPSEVRRCTWFYKGDKDNKYVPYSESFSQVLEDTYMLAVTLDEWKKKIESPNREI
                     IVLHNPKLMVHYQPIAGSDEWGSTSTEQGRPRSVKRGVENIPVDIHCGEPLQIDHLVF
                     VVHGIGPACDLRFRSIVQCVNDFRSVSLNLLQTHFKKAQENEQIGRVEFLPVNWHSPL
                     HSTGVDIDLQRITLPSINRLRHFTNDTILDVFFYNSPTYCQTIVDTVASEMNRIYTLF
                     LQRNPDFKGGVSIAGHSLGSLILFDILTNQKNSIGDIDSEKGSLSSAEDRGDASTLEE
                     DLKKLQLSEFVTVFEKEKVDREALALCTDRDLQEMGIPLGPRKKILNHFSARKNSVSI
                     NRPAMSASEVNISKENGDYLDVGIGQVSVKYPRLNYKPEIFFAFGSPIGMFLTVRGLR
                     RIDPNYKFPTCKGFFNIYHPFDPVAYRIEPMVAPGIEFEPMLIPHHKGRKRMHLELRE
                     GLTRMSMDLKNNLLGSLRMAWKSFTRGPYPALQASETAEETEAEPESSSEKSNEANTE
                     EPPVEVKEEAPISVGMLNGGQRIDYVLQEKPIESFNEYLFALQSHLCYWESEDTVLLV
                     LKEIYQTQGVFLDQPLQ"
     exon            321..511
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            512..601
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            602..722
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            723..812
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            813..948
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1157
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1225
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1348
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1444
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1445..1528
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1684
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1685..1787
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1788..1958
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1959..2118
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2226
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     exon            2227..4266
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4218..4223
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /note="hexamer: AATAAA"
     polyA_site      4244
                     /gene="Ddhd2"
                     /gene_synonym="2010305K11Rik; mKIAA0725; SAMWD1"
                     /note="major polyA site"
ORIGIN      
        1 cccggcttgg gaggtaggct gcagcggcga tgtggcccgg gctccctccg cgagggctga
       61 gagcgcgagc ggcggtgtgg acgagcgagt ccagagcgaa atgtcatcgg gggaatcaca
      121 ccaggagcag ctgtctcagt cagatccgtc cccatccccg aactcttgta gttccttcga
      181 actcatagac atggacgcca gtagttccta tgaacccgtt tctccacatt ggttttattg
      241 taaggtacta gattccaagg agctctggat tcctttcaac tctgaggatt cacagcagct
      301 ggaagacgca tatggctctg gaaaagattg taatgagagg attgttccca ccgatggggg
      361 cagatatgat gttcatttag gggagcggat gcggtatgct gtgtactggg acgagctgcc
      421 atcggaagtg agacggtgta cgtggtttta caagggagac aaagacaata agtatgtccc
      481 ctactcggag agcttcagcc aagtcttgga ggatacgtac atgcttgcag taactctgga
      541 tgaatggaaa aaaaaaatag aatctccaaa cagagaaatt attgtattac acaacccaaa
      601 gctcatggtg cattaccagc caattgcagg gtctgatgaa tggggttcaa catccactga
      661 gcaaggtcgg ccaagatcag taaaaagagg cgtcgagaac atccctgttg atattcactg
      721 tggggaaccg ttacaaatag atcacttagt gtttgtagtc catgggattg gcccagcttg
      781 cgatcttcgt tttcgaagca ttgttcaatg tgttaatgat tttcgaagtg tttccttgaa
      841 cctgctccaa acacatttta agaaagccca agaaaatgag cagatcggaa gggtggagtt
      901 tcttccagtc aactggcaca gtcctttgca ttctactggc gtggacatag atctgcagcg
      961 gataacccta cccagtatca atcgcctcag acacttcact aatgatacaa ttctggatgt
     1021 cttcttctac aatagcccca cctactgtca gactattgtg gacacagtag cttctgaaat
     1081 gaaccgaata tatacacttt ttctgcagag gaaccctgat ttcaaagggg gtgtatccat
     1141 tgctggtcat agtttaggtt cgcttatatt gtttgatatc ctaacaaatc agaaaaattc
     1201 tattggggat attgacagtg aaaagggttc gttgagctct gctgaggatc gaggcgatgc
     1261 atcaacatta gaagaagact tgaagaagct tcaactctct gagtttgtca ctgtctttga
     1321 gaaggagaaa gttgacagag aagctctggc tttatgtaca gacagagatc ttcaggaaat
     1381 ggggattccc ttaggaccca gaaagaagat actgaaccat ttcagtgcca ggaagaattc
     1441 agtgagtatt aatagaccag ccatgtcagc ctctgaggta aacatctcca aggaaaatgg
     1501 tgactatctg gatgttggca ttgggcaggt gtctgtaaaa tacccccggc tcaactacaa
     1561 accagagatc ttctttgcct ttggctcccc catcggaatg tttcttactg tccgagggtt
     1621 gagaagaatt gatcctaatt ataaatttcc cacatgcaaa ggtttcttca atatttacca
     1681 tccatttgac cctgtggcat ataggattga gccaatggtg gccccaggaa tagagtttga
     1741 gccaatgctg atcccacacc ataaaggcag gaagcggatg cacttagaac tgagagaggg
     1801 cttgaccagg atgagtatgg acctgaagaa caacttgctg gggtcactga ggatggcctg
     1861 gaagtctttc accagaggcc cataccctgc cttacaagcc tcagaaactg cagaagaaac
     1921 tgaggcagaa cccgaatcaa gttcagaaaa gtccaatgaa gccaacacgg aggagcctcc
     1981 tgtggaagtt aaagaagagg ctcccatcag tgtgggcatg ctgaatggag gccagcggat
     2041 tgactacgtg ctacaagaga agcccattga gagtttcaat gaatatttat ttgctttaca
     2101 aagccatcta tgctactggg aatctgaaga tacagtattg ctggttctca aagagatcta
     2161 ccaaacccag ggtgtcttcc ttgatcagcc tttacagtaa acatgaccca tctgtggctg
     2221 cttaatatgg acactgaggg agactgtcct gaaaacccac acgcttgctg ctgcagcttc
     2281 cttctcagct accttgtgtc ctgcatgctg cctgccttac tccatgggat cttcccaaga
     2341 gaaccctctt tggagactac tggaaagcgg aggggcactc actgttctgt atggacgctc
     2401 agcaggtttt gtggctaggg cgtttacata tcccaatgct ttaacagcag cacttccttg
     2461 tttcttacat gggaatgaat atctgtttag ggggcttggg gggggttact ccttatcctt
     2521 accaacacta cgggtgggag tataagatgt agaggtactt aggggtcccg tccctctgtg
     2581 tgttctctgt gctggagggc tgagcatcac tgcatttggg agagctgctg gcttttgagc
     2641 cagcttttct ttcctggatt atatgaagat cagacagaag cttactaata gcacagtttg
     2701 ctctgaacac tgttacgtga gtggattgta gaaccactgg tgctggtcct ggtcctggtc
     2761 cttacaactg cttcagcttc tgcaaccctg aaggttgtat taactgtgtg cctgctcttt
     2821 tacaccgtat tccaagccag cggtgaagtt gaggacatta ctaggtaata cttattttga
     2881 aggtcaacct gaaattacat ggcccttccc tgctgttcat gttgtaaatg cagtctttta
     2941 gtagcaccga tttaagtcac ctgctccttc cacttgagta aaggaagtat gtgacataaa
     3001 tgtcacattt tttttttttt ttttttttga cacagggttt ctctgtgtag ccctggttgt
     3061 cctggaactc actctgtaga ccaggctggc ctcagaaatc cgcctgcctc tgcctctgcc
     3121 tcccgagtgc tgggatcaaa ggcgtgcgcc accacgcccg gctgcgtcac tgctttctga
     3181 ggtaccatca aattgtttat tacagttggc agtcatggat ttgagcatgc aagctaggct
     3241 accttctgaa ggaaaaccct gtctacaggg tctgaggagc tgaatctgga taactcaagg
     3301 cacattgatc gtgaggaagg gtgactgtac actaagtgtt cccttcctta gcatgtgccg
     3361 tcatctttgt cctcctcacg attgcccttt agctcctctg atttccacag gtctctatag
     3421 agtggcagga gaggtgagct gtgttttggt ttttttcctt gttttgtaaa cctatcatgg
     3481 tatccagagg cactgcacat aacatgccct gaaaccctca gatggcagtg ccctctacag
     3541 acaggcaaag ggagggaagc actgtgccct ggacgttaga gaggcaagca tggtacgtca
     3601 ggcccagcct gccttcctgc tcatgggtaa tactggatga gcaagctact aaggagttcc
     3661 aggtatatgg aatactttat tttttctaca atatgtcttg agtaagaata cctgagaatt
     3721 aagtagtata ttcattaaat actaaaaagt aagttttgtc aattgctgtc ttgaggtttg
     3781 tttactcttg ttagcagagt cattactact taagagtaaa ccaaagtaca accaagagtg
     3841 cgttcttgtg accaaagatg ggcagcttgg ccctggagag ctagataaac ccctgcactg
     3901 agaaaggctt cttcccgcag tccggtactt acacgtgacc acgctgagtg ttatacaagg
     3961 cagtgtggtg gcaagggacc tgggtgtgga cctgcactgc gtcgtctgtt atcatgcaca
     4021 atctcctgac caaggctgtt tctaaactcc agggtgacag cattctgtct gtgattctgt
     4081 ctgtgattct ggcagcacag ttggtgggag actgtctgca agggacgatg agtagctctg
     4141 ataaccttgc tgatattttg cacttttgaa atgtttgttt tatatgtgat tatatttaag
     4201 acgttagtaa atgccgaaat aaaactattg accatttgcc tacattcatg tggccttatc
     4261 tatcat
//
