LOCUS       NM_172928               3498 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus doublecortin-like kinase 3 (Dclk3), mRNA.
ACCESSION   NM_172928 XM_911635
VERSION     NM_172928.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   de Longprez,L., Gaillard,M.C., Decraene,C., Perot,J.B., Keime,C.,
            Nadkarni,N., Louca,M., Jan,C., Liot,G., Auregan,G., Guillermier,M.,
            Mitja,J., Hantraye,P., Bertaux,F., Norbert,M., Bonvento,G.,
            Flament,J., Dhenain,M., Neri,C., Bemelmans,A.P., Merienne,K.,
            Cambon,K. and Brouillet,E.
  TITLE     Loss of the neuronal kinase DCLK3 leads to anxiety-like behaviour
            and memory deficits
  JOURNAL   Brain (2025) In press
   PUBMED   39908340
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Galvan,L., Francelle,L., Gaillard,M.C., de Longprez,L., Carrillo-de
            Sauvage,M.A., Liot,G., Cambon,K., Stimmer,L., Luccantoni,S.,
            Flament,J., Valette,J., de Chaldee,M., Auregan,G., Guillermier,M.,
            Josephine,C., Petit,F., Jan,C., Jarrige,M., Dufour,N., Bonvento,G.,
            Humbert,S., Saudou,F., Hantraye,P., Merienne,K., Bemelmans,A.P.,
            Perrier,A.L., Deglon,N. and Brouillet,E.
  TITLE     The striatal kinase DCLK3 produces neuroprotection against mutant
            huntingtin
  JOURNAL   Brain 141 (5), 1434-1454 (2018)
   PUBMED   29534157
  REMARK    GeneRIF: This study suggest that the presence of DCLK3 in striatal
            neurons may play a key role in transcription regulation and
            chromatin remodelling in these brain cells, and show that reduced
            expression of the kinase in Huntington's disease could render the
            striatum highly vulnerable to neurodegeneration.
REFERENCE   3  (bases 1 to 3498)
  AUTHORS   Molyneaux,B.J., Goff,L.A., Brettler,A.C., Chen,H.H., Hrvatin,S.,
            Rinn,J.L. and Arlotta,P.
  TITLE     DeCoN: genome-wide analysis of in vivo transcriptional dynamics
            during pyramidal neuron fate selection in neocortex
  JOURNAL   Neuron 85 (2), 275-288 (2015)
   PUBMED   25556833
  REMARK    Erratum:[Neuron. 2016 Jan 6;89(1):235]
REFERENCE   4  (bases 1 to 3498)
  AUTHORS   Dijkmans,T.F., van Hooijdonk,L.W., Fitzsimons,C.P. and
            Vreugdenhil,E.
  TITLE     The doublecortin gene family and disorders of neuronal structure
  JOURNAL   Cent Nerv Syst Agents Med Chem 10 (1), 32-46 (2010)
   PUBMED   20236041
  REMARK    Review article
REFERENCE   5  (bases 1 to 3498)
  AUTHORS   Reiner,O., Coquelle,F.M., Peter,B., Levy,T., Kaplan,A., Sapir,T.,
            Orr,I., Barkai,N., Eichele,G. and Bergmann,S.
  TITLE     The evolving doublecortin (DCX) superfamily
  JOURNAL   BMC Genomics 7, 188 (2006)
   PUBMED   16869982
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3498)
  AUTHORS   Ohmae,S., Takemoto-Kimura,S., Okamura,M., Adachi-Morishima,A.,
            Nonaka,M., Fuse,T., Kida,S., Tanji,M., Furuyashiki,T., Arakawa,Y.,
            Narumiya,S., Okuno,H. and Bito,H.
  TITLE     Molecular identification and characterization of a family of
            kinases with homology to Ca2+/calmodulin-dependent protein kinases
            I/IV
  JOURNAL   J Biol Chem 281 (29), 20427-20439 (2006)
   PUBMED   16684769
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BB617821.1, DQ286388.1 and
            AV341123.2.
            
            On Jun 2, 2009 this sequence version replaced NM_172928.4.
            
            Summary: This gene encodes a member of the protein kinase
            superfamily and the doublecortin family. Differently from the other
            two closely related family members (DCLK1 and DCLK2), the protein
            encoded by this gene contains only one N-terminal doublecortin
            domain and is unable to bind microtubules and to regulate
            microtubule polymerization. The protein contains a C-terminal
            serine/threonine protein kinase domain, which shows substantial
            homology to Ca2+/calmoduline-dependent protein kinase, and a
            serine/proline-rich domain in between the doublecortin and the
            protein kinase domains, which mediates multiple protein-protein
            interactions. [provided by RefSeq, Sep 2010].
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus to one that is supported by available conservation data.
            This would result in a protein that is 171 aa longer at the
            N-terminal.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK135010.1, DQ286388.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                BB617821.1         2-33
            33-2998             DQ286388.1         2-2967
            2999-3498           AV341123.2         130-629
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 61.33 cM"
     gene            1..3498
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /note="doublecortin-like kinase 3"
                     /db_xref="GeneID:245038"
                     /db_xref="MGI:MGI:3039580"
     exon            1..272
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..2557
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /EC_number="2.7.11.1"
                     /note="doublecortin and CaM kinase-like 3; CLr; CLICK-I
                     and II-related; doublecortin-like and CAM kinase-like 3;
                     Click-I, -II related"
                     /codon_start=1
                     /product="serine/threonine-protein kinase DCLK3"
                     /protein_id="NP_766516.2"
                     /db_xref="CCDS:CCDS23585.2"
                     /db_xref="GeneID:245038"
                     /db_xref="MGI:MGI:3039580"
                     /translation="MPAAPVLRPPPPPATPAPPAPSRPAPPIPGHRGPCDHSLKCLSS
                     KISERKLPGPWLPAGRGPLEKPVLGPRGAVMPLFSPQSSLHSVRAEHSPLKPRVVTVV
                     KLGGQPLRKATLLLNRRSVQTFEQLLSDISEALGFPRWKNDRVRKLFTLKGREVKSVS
                     DFFREGDAFIAMGKEPLTLKSIQLAMEELYPKNRALALAPHSRVPSPRLRSRLPSKLL
                     KGSHRCGEAGSYSAEMESKAVSRHQGKTSTVLAPEDKARAQKWVRGKQESEPGGPPSP
                     GAATQEETHASGEKHLGVEIEKTSGEIVRCEKCKRERELQLGLQREPCPLGTSELDLG
                     RAQKRDSEKLVRTKSCRRPSEAKSTDGEEGWKGDSHRGSPRDPPQELRRPNSNSDKKE
                     IRGSESQDSHPQGAPKAQKDLVEGPPAVEEGPIDMRREDRHTCRSKHAAWLRREQQAE
                     PPQLPRTRGEEKQAEHEKKPGGLGERRAPEKESKRKLEEKRPERPSGRKPRPKGIISA
                     DVEKHYDIGGVIGDGNFATVKECRHRETKQAYAMKMIDKSQLKGKEDIVDSEILIIQS
                     LSHPNIVKLHEVYETEAEIYLIMEYVQGGDLFDAIVENVKFPEPEAAVMITDLCKALV
                     HMHDKNIVHRDVKPENLLVQRNEDKSITLKLADFGLAKYVVRPIFTVCGTPTYVAPEI
                     LSEKGYGLEVDMWAAGVILYILLCGFPPFRSPERDQDELFNIIQVGQFEFLSPYWDNI
                     SDAAKDLVRNLLEVDPKKRYTAEQVLQHPWIEMVGHTNTGNSQKEESPNS"
     exon            273..2110
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2243
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..2411
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /inference="alignment:Splign:2.1.0"
     exon            2412..3498
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2981..2986
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /note="hexamer: AATAAA"
     polyA_site      3005
                     /gene="Dclk3"
                     /gene_synonym="C730036H08; Dcamkl3"
                     /note="major polyA site"
ORIGIN      
        1 agcgcggttg tcaagctcgc gctgaggaga ctgtccttgc gccgtggctg ccgccagcag
       61 caacctaaag gaagaggaga cctggatcgc tctgtgcagc gagtagtccc ggcgccttcc
      121 tgcgcccgct tggccgccgt cgggccacgt ccaagagctg ccacctgcag ccaggccgcc
      181 aaccatgcca gccgcgccgg tcctgcgccc gccgccgccg ccagcgaccc ccgccccgcc
      241 ggcacccagt cgccctgcgc ctcccattcc gggccaccga ggcccatgtg accattctct
      301 gaaatgctta agctcgaaga tctctgagag aaagctgcca ggcccctggt tacctgcggg
      361 acgaggacct ctggagaagc cagttctggg gccacgtggt gccgtcatgc cgctgttcag
      421 ccctcagagc agcctccact cagtccgcgc agagcacagc ccactgaagc ccagggtggt
      481 gacggtggtg aagctgggtg ggcagcccct ccgtaaggcc accctgctcc tcaaccggcg
      541 ctcagtgcag acctttgagc agctcctatc agacatctcc gaagccttgg gcttcccacg
      601 ctggaagaac gaccgtgtgc ggaagctgtt caccctcaag ggcagggagg tgaagagtgt
      661 gtctgacttc ttccgggagg gtgatgcttt catagctatg ggcaaagagc cgctgacatt
      721 gaagagtatc cagttggcca tggaggagct gtatcctaag aaccgggctc ttgccctggc
      781 ccctcacagt agagtcccct ccccaaggct gagaagcaga cttcccagca agcttctgaa
      841 aggaagtcac cgctgtgggg aggcaggaag ctatagcgcg gaaatggaga gtaaggcagt
      901 ctctaggcat cagggcaaga cttccacagt gctggcccca gaagacaagg cgagggccca
      961 gaagtgggta agagggaaac aggagtcaga acctggtggc ccgccttcac ccggggcagc
     1021 cactcaggag gagactcatg caagtggaga gaaacatctg ggggtggaga tcgaaaagac
     1081 ctccggggag attgtcagat gtgagaagtg taagagagaa agagagctgc agttgggcct
     1141 gcagagggag ccgtgcccgc tgggaaccag tgagctggac ctggggagag ctcagaagag
     1201 ggattccgag aagttggtga ggaccaagag ctgcaggagg ccttctgagg caaaatctac
     1261 agatggagag gaagggtgga agggtgacag ccatcggggc agtcccaggg acccccctca
     1321 ggaactgagg aggcccaaca gcaactcaga caagaaagag atcagaggct cagaaagtca
     1381 ggacagtcat cctcaggggg cacccaaggc ccagaaggac ctcgtggaag ggccaccagc
     1441 tgtagaggag gggccgatag acatgaggag agaggaccgg cacacatgca ggagcaagca
     1501 tgccgcctgg ctccggagag agcagcaggc cgaaccccca cagctcccca gaacccgagg
     1561 ggaggagaag caagcagagc acgagaagaa gccaggcggc ttaggagaga ggagggcgcc
     1621 agagaaggag tctaagagga agctagaaga gaagaggcca gaacgaccca gtggccggaa
     1681 gccgaggccc aagggcatca tctcagcgga tgtggagaag cactatgaca taggtggggt
     1741 cattggggat ggcaactttg ccaccgtgaa ggaatgcagg caccgagaga ccaagcaggc
     1801 ttacgccatg aagatgattg acaagtccca gctgaagggt aaggaggaca ttgtcgacag
     1861 tgagatttta atcatccaga gtctctctca tcccaacatt gtgaaactgc acgaggtcta
     1921 cgagacggag gcggagatct acctgatcat ggagtatgtg cagggagggg acctttttga
     1981 tgccatcgtt gaaaatgtga agtttccaga gcccgaggct gcagttatga tcacagactt
     2041 gtgtaaggcc ctcgtccaca tgcacgacaa gaatatcgtc caccgggacg tgaaaccaga
     2101 aaacctcctg gttcagcgaa atgaagacaa gtctatcacc ttgaagctgg ctgattttgg
     2161 cttggccaaa tatgtggtga ggcctatatt tactgtgtgt gggacgccaa catatgtagc
     2221 tcctgaaatt ctttctgaga aaggttacgg cctggaggtg gacatgtggg cggcaggtgt
     2281 gatcctatac atcctcttgt gtggcttccc ccctttccga agtcctgaga gggaccaaga
     2341 cgagctcttc aacatcatcc aagtgggcca gtttgagttc ctctctcctt actgggacaa
     2401 catttctgat gctgccaaag atctggtgag aaatttgctg gaggtggacc ctaagaagcg
     2461 gtacacggcc gaacaggtcc tacagcatcc ctggattgag atggttgggc ataccaacac
     2521 agggaactca cagaaggagg agtcccccaa cagttagggt cacttccaga gtcagcacaa
     2581 gaaggttgca gagcagatgc cataatcctg cctgggacat ctgtccagcc tggtctcctt
     2641 gaggacagag aagagagggg acgtctagtc tacaagcaag gctgtgcaag acccttctcc
     2701 acagaattag ggaaggagaa gagccacctg gcatatttta aagagtgttt gaaagcaatg
     2761 ttctaatgtt gaatgatgta tgatattttt agatttgtat atctgaagtc tttaatacct
     2821 tttgggtaag aattgctatg aatgaggaat tttgataata atgacagatt ctgttttctc
     2881 cttgtattca catccatggt aggctacagg agtgtgcttc acatcgctca agctccccca
     2941 cacaatgaat gaggtgattc ctggcttttt taaaaatctc aataaaacac aatgaataac
     3001 agaaagatct gccttgttca atggtgggga gagctgggga agtagttcaa cacagctgtg
     3061 ttttaacctt tgacaaagag accaatacat atggttctat gaactgaaat tccttgaaaa
     3121 aaaacccaac atttaaaaaa aataatctgc aggttgactg attagaactt catgattatg
     3181 gagaattgga atgtaaagat tgagagtcaa aaattacctt gggttatctt ccatcccctt
     3241 aatagccatg taccaccaaa ctgtatgtcc aacctaacag ataaaactgt tggaacgtat
     3301 taactcagct gagcactagc ttctactaac ccaaagctaa gatgtgatag cacctaaagc
     3361 ctgcagaaga gacatctccg ctttgctgta agctgcctgc ctgaccacac caccagtata
     3421 tgtagtaaat tcattgatct gagactttgt tttatctgac gataataata ataataataa
     3481 aatcatgtaa ccatttct
//
