LOCUS       NM_027539               4047 bp    mRNA    linear   ROD 04-OCT-2024
DEFINITION  Mus musculus doublecortin-like kinase 2 (Dclk2), transcript variant
            2, mRNA.
ACCESSION   NM_027539
VERSION     NM_027539.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Sun,M., Huang,X., Ruan,X., Shang,X., Zhang,M., Liu,L., Wang,P.,
            An,P., Lin,Y., Yang,J. and Xue,Y.
  TITLE     Cpeb4-mediated Dclk2 promotes neuronal pyroptosis induced by
            chronic cerebral ischemia through phosphorylation of Ehf
  JOURNAL   J Cereb Blood Flow Metab 44 (9), 1655-1673 (2024)
   PUBMED   38513137
  REMARK    GeneRIF: Cpeb4-mediated Dclk2 promotes neuronal pyroptosis induced
            by chronic cerebral ischemia through phosphorylation of Ehf.
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4047)
  AUTHORS   Li,L., Ruan,X., Wen,C., Chen,P., Liu,W., Zhu,L., Xiang,P.,
            Zhang,X., Wei,Q., Hou,L., Yin,B., Yuan,J., Qiang,B., Shu,P. and
            Peng,X.
  TITLE     The COMPASS Family Protein ASH2L Mediates Corticogenesis via
            Transcriptional Regulation of Wnt Signaling
  JOURNAL   Cell Rep 28 (3), 698-711 (2019)
   PUBMED   31315048
REFERENCE   4  (bases 1 to 4047)
  AUTHORS   Sandberg,M., Flandin,P., Silberberg,S., Su-Feher,L., Price,J.D.,
            Hu,J.S., Kim,C., Visel,A., Nord,A.S. and Rubenstein,J.L.R.
  TITLE     Transcriptional Networks Controlled by NKX2-1 in the Development of
            Forebrain GABAergic Neurons
  JOURNAL   Neuron 91 (6), 1260-1275 (2016)
   PUBMED   27657450
REFERENCE   5  (bases 1 to 4047)
  AUTHORS   Tuy,F.P., Saillour,Y., Kappeler,C., Chelly,J. and Francis,F.
  TITLE     Alternative transcripts of Dclk1 and Dclk2 and their expression in
            doublecortin knockout mice
  JOURNAL   Dev Neurosci 30 (1-3), 171-186 (2008)
   PUBMED   18075264
REFERENCE   6  (bases 1 to 4047)
  AUTHORS   Bailey,P.J., Klos,J.M., Andersson,E., Karlen,M., Kallstrom,M.,
            Ponjavic,J., Muhr,J., Lenhard,B., Sandelin,A. and Ericson,J.
  TITLE     A global genomic transcriptional code associated with CNS-expressed
            genes
  JOURNAL   Exp Cell Res 312 (16), 3108-3119 (2006)
   PUBMED   16919269
REFERENCE   7  (bases 1 to 4047)
  AUTHORS   Reiner,O., Coquelle,F.M., Peter,B., Levy,T., Kaplan,A., Sapir,T.,
            Orr,I., Barkai,N., Eichele,G. and Bergmann,S.
  TITLE     The evolving doublecortin (DCX) superfamily
  JOURNAL   BMC Genomics 7, 188 (2006)
   PUBMED   16869982
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4047)
  AUTHORS   Ohmae,S., Takemoto-Kimura,S., Okamura,M., Adachi-Morishima,A.,
            Nonaka,M., Fuse,T., Kida,S., Tanji,M., Furuyashiki,T., Arakawa,Y.,
            Narumiya,S., Okuno,H. and Bito,H.
  TITLE     Molecular identification and characterization of a family of
            kinases with homology to Ca2+/calmodulin-dependent protein kinases
            I/IV
  JOURNAL   J Biol Chem 281 (29), 20427-20439 (2006)
   PUBMED   16684769
REFERENCE   9  (bases 1 to 4047)
  AUTHORS   Fenske,T.S., McMahon,C., Edwin,D., Jarvis,J.C., Cheverud,J.M.,
            Minn,M., Mathews,V., Bogue,M.A., Province,M.A., McLeod,H.L. and
            Graubert,T.A.
  TITLE     Identification of candidate alkylator-induced cancer susceptibility
            genes by whole genome scanning in mice
  JOURNAL   Cancer Res 66 (10), 5029-5038 (2006)
   PUBMED   16707424
REFERENCE   10 (bases 1 to 4047)
  AUTHORS   Koizumi,H., Tanaka,T. and Gleeson,J.G.
  TITLE     Doublecortin-like kinase functions with doublecortin to mediate
            fiber tract decussation and neuronal migration
  JOURNAL   Neuron 49 (1), 55-66 (2006)
   PUBMED   16387639
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BQ771344.1, BC056921.1 and
            BY361646.1.
            
            On Sep 14, 2010 this sequence version replaced NM_027539.4.
            
            Summary: This gene encodes a member of the protein kinase
            superfamily and the doublecortin family. The protein encoded by
            this gene contains two N-terminal doublecortin domains, which bind
            microtubules and regulate microtubule polymerization, a C-terminal
            serine/threonine protein kinase domain, which shows substantial
            homology to Ca2+/calmoduline-dependent protein kinase, and a
            serine/proline-rich domain in between the doublecortin and the
            protein kinase domains, which mediates multiple protein-protein
            interactions. The microtubule-polymerizing activity of the encoded
            protein is independent of its protein kinase activity. This gene
            and the DCX gene, another family member, share function in the
            establishment of hippocampal organization and their absence results
            in a severe epileptic phenotype and lethality, as described in
            human patients with lissencephaly. Multiple alternatively spliced
            transcript variants encoding different isoforms have been
            identified. [provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (2) is an exon-5-lacking
            transcript (PMIDs:15611072 and 18075264). It lacks an in-frame
            coding exon and has an additional 3 nts at an alternate splice
            site, as compared to variant 1. The resulting isoform (2) is
            shorter; it lacks an internal segment but has a downstream extra
            aa, as compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC056921.1, SRR7652917.415965.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-617               BQ771344.1         1-617
            618-4044            BC056921.1         586-4012
            4045-4047           BY361646.1         185-187
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 37.88 cM"
     gene            1..4047
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /note="doublecortin-like kinase 2"
                     /db_xref="GeneID:70762"
                     /db_xref="MGI:MGI:1918012"
     exon            1..1135
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     CDS             715..2985
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     doublecortin and CaM kinase-like 2;
                     serine/threonine-protein kinase DCLK2; doublecortin-like
                     and CAM kinase-like 2; caMK-like CREB regulatory kinase 2"
                     /codon_start=1
                     /product="serine/threonine-protein kinase DCLK2 isoform 2"
                     /protein_id="NP_081815.3"
                     /db_xref="CCDS:CCDS17448.1"
                     /db_xref="GeneID:70762"
                     /db_xref="MGI:MGI:1918012"
                     /translation="MASTRSIELEHFEERDKRPRPGSRRGAPSSSGGSSISGPKGNGL
                     IPSPAHSAHCSFYRTRTLQALSSEKKAKKARFYRNGDRYFKGLVFAISNDRFRSFDAL
                     LIELTRSLSDNVNLPQGVRTIYTIDGSRKVTSLDELLEGESYVCASNEPFRKVDYTKN
                     VNPNWSVNIKGGTTRTLAVASAKSEVKESKDFIKPKLVTVIRSGVKPRKAVRILLNKK
                     TAHSFEQVLTDITEAIKLDSGVVKRLCTLDGKQVTCLQDFFGDDDVFIACGPEKYRYA
                     QDDFVLDHSECRVLKSSYSRASAAKYSGSRSPGFSRRSKSPASVNGTPSSQLSTPKST
                     KSSSSSPTSPGSFRGLKQISAQGRSSSNVNGGPELDRCLSPEGVNGNRCSESFPLLEK
                     YRIGKVIGDGNFAVVKECVDRYTGKEFALKIIDKAKCCGKEHLIENEVSILRRVKHPN
                     IIMLVEEMETATDLFLVMELVKGGDLFDAITSSTKYTERDGSAMVYNLANALRYLHSL
                     SIVHRDIKPENLLVCEYPDGTKSLKLGDFGLATVVEGPLYTVCGTPTYVAPEIIAETG
                     YGLKVDVWAAGVITYILLCGFPPFRSENNLQEDLFDQILAGKLEFPAPYWDNITDSAK
                     ELISQMLQVNVEARCTAGEILSHPWVSDDASQENNMQAEVTGKLKQHFNNALPKQNST
                     TTGVSVIMNTALDKEGQIFCSKLCQDSSRPSREQTSPVPPSAQEAPPPLESPRPPGPP
                     ATSGCDLAGTWRRHRD"
     exon            1136..1467
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1468..1570
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1672
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1673..1767
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1843
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1844..1952
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1953..2010
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..2130
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2277
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2411
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2412..2489
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2490..2586
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2587..2667
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2668..2784
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2785..4047
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4020..4025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /note="hexamer: AATAAA"
     polyA_site      4044
                     /gene="Dclk2"
                     /gene_synonym="6330415M09Rik; CL2; Click-II; CLICK2;
                     Dcamkl2"
                     /note="major polyA site"
ORIGIN      
        1 cctcgcgcgg cgccgccctc ttcctcacca tctccctctc ggtgtggttt gtggtgtccc
       61 tgaagggaca gagggtgtcc tccggggtcc aggccctgac aggcggccga gagcgcggat
      121 gcccgtgggc gtgcgtgcgt gggaggcggc tgcactcttc ccgcaggggc ccagcgcctc
      181 tagaggatcc ccggccaggg acgcgcaccg gccgtggact gggcctgggc gcgcgcgacg
      241 cggcggcggg aacccgggac cccaggccgc gccgggcagc cccgaggcgc ctcgcccacg
      301 ctcgcgcccc tcccccctcc gcttccttcc tccactcagc gcgcccgaga gcgcaggccg
      361 gatgcgaacc cgggccagcc tagggtcgtt caacttggcg gctcccggcc tgctgcccgc
      421 gcgctacttg tgaggcgtgg cccggcgaag gaggcgcttg gggggcgtgg ggctcctcag
      481 ctggcggcgg cgaccgaggg cgcgcgggac aatgagctgg gctgctgcgc gctgcggccg
      541 cctttagctg gctgagcggc tccacctgcg agcatggccc gaatgcccgc gccaggtgtc
      601 ccgctgcttt aagacgggac ggagcgccta gatcgcggcg cgctgcggcc ggggcggttg
      661 ggacgtcctt ttattaatta attaatttgt ggagagctct cccgagcagc cgcgatggcc
      721 agcacaagga gcattgagct ggaacatttt gaagaacggg acaaaaggcc gcggccaggg
      781 tcacggagag gggctcccag ctcctccggg ggcagcagca tctctggccc caagggcaac
      841 gggctcatcc ccagcccggc gcacagtgct cactgcagct tctaccgcac gcggaccttg
      901 caggccctca gctcggagaa gaaggccaag aaggcgcggt tctaccggaa tggggaccgc
      961 tatttcaaag gcctggtctt tgccatctcc aacgaccgtt tccgttcctt cgatgcgctc
     1021 ctcatagagc tcacccgctc actgtctgac aatgtgaacc tgccccaggg cgtccgcacc
     1081 atctacacca tagacggcag ccggaaggtc accagcctgg acgagctgct ggaaggtgaa
     1141 agttacgtgt gtgcctccaa cgaaccattt cgtaaagttg attacaccaa aaacgttaat
     1201 ccgaactggt ctgtgaacat caagggcgga accacccgca ccctggccgt ggcctctgcg
     1261 aagagtgagg tgaaagaaag caaagacttc attaaaccca agttagtgac tgtgattcga
     1321 agcggagtga agcctagaaa ggccgtgcgg atccttctga ataaaaagac tgcccattcc
     1381 tttgagcagg tcttaacaga tatcaccgaa gccattaaac tagactcagg cgtggtcaag
     1441 aggctgtgca ccctggatgg aaagcaggtt acttgtctgc aagacttttt tggagacgat
     1501 gatgttttta ttgcatgtgg acctgaaaaa tatcgttatg cccaagatga ctttgtcctg
     1561 gatcatagcg aatgccgtgt cctgaaatcg tcctactctc gagcctcagc tgcgaagtat
     1621 tctggatcca gaagcccagg gttctcccgc cgcagcaagt caccagcttc agtgaatgga
     1681 actcccagta gccagctttc cactccgaag tccaccaagt cctccagctc ctctccaacc
     1741 agcccgggaa gtttcagagg attgaagcag atttctgctc agggcagatc ttcttccaac
     1801 gtaaacggtg ggcctgaact tgaccgttgc ctgagccctg aaggtgtgaa tggaaaccgg
     1861 tgctccgagt cgttccccct tctggagaaa tacagaatag ggaaggtcat cggggacggc
     1921 aacttcgcgg tagttaagga gtgcgtggac aggtacactg gaaaagagtt tgcattaaag
     1981 attatagaca aagccaaatg ctgtggaaag gagcatctga ttgagaacga agtgtcaatc
     2041 ctgcgccgag tgaagcaccc caacatcatc atgttggttg aagagatgga aacagcaact
     2101 gacctctttc tagtgatgga actggtcaaa ggtggagatc tctttgatgc gattacctct
     2161 tcaaccaagt acactgagag agatggaagc gccatggtgt acaacctagc caatgccctc
     2221 cggtacctgc acagcctcag catcgtccac agggacatca agcctgagaa tctgctggtg
     2281 tgcgaatacc cagatggaac caagtctttg aagctgggag actttgggct ggcgacggtg
     2341 gttgaaggcc cgttgtacac ggtctgtggc acgccaactt atgtggcacc agagatcata
     2401 gctgaaacag gttatggcct gaaggtggat gtttgggcag ctggtgtgat tacatacata
     2461 cttctctgtg gattcccacc attccggagt gagaacaatc tccaggaaga tctctttgac
     2521 cagatcttgg ctggaaagct ggaattccca gccccctact gggacaacat tacagactct
     2581 gccaaggagt taatcagtca aatgcttcag gtaaacgttg aagctcgctg tactgcggga
     2641 gaaattctga gtcacccctg ggtgtcagat gatgcatccc aggagaacaa tatgcaagcc
     2701 gaggttacag gtaaactaaa acagcacttt aataatgcgc tccccaaaca gaacagcacc
     2761 accaccgggg tctccgttat catgaacacg gctctagata aagaggggca gattttctgc
     2821 agcaagctct gtcaagacag cagcagacca tccagggagc agacctcgcc agtccctccc
     2881 tcagcccagg aggcccctcc cccactggag tctcccaggc cccctggtcc tccagccacc
     2941 tctggctgtg atctggcagg gacctggcgc cgccaccgag actgaaccct gtgtacacag
     3001 gccaaagccg ctcaccgtgc caggcaggcc gctcttcagc gggcctcatc aactccttgc
     3061 tgaaagagcc tctctctcct ggctgctgct ttgacagttt gttggcatgt tctgtcactg
     3121 cctgagaaca gaactggggc atcctggagc ccgtgtgcag catggcctca tgcactttgg
     3181 gctctgccct ctctagttcc tggccatgtc aagcaagcca tgtcagttgt ggagacatat
     3241 ttggagtgac tcattttaaa tcatctgtgt ttttagttca gtagacactt ggccgctttc
     3301 cccagagaat gtgcaacttt tatctggaat tacatcgttt ttacagaaat gcattggacg
     3361 aattaggatt ctccttattg aaggacactc caagtgctcc cttctcagga cacagctatg
     3421 gttcctttgt tctgtgagag gcaacatggc ctcttcgtct tcatagggcg ctgaggcacc
     3481 tggcttctga ctaggagtgt cattctgact tgagagggtc atttgcctcc tgactttggg
     3541 aagtgcattc tgaagcccca ggaagcagat cccgaccatc cgtccgtccg tctgtccgct
     3601 gggctggggt gctgggctcg cagacctagc tgactcaccc ttggtttccc gactctcctt
     3661 gtgtgtgttt taggaaatgc ttatgaagct ggcccgtggg cttcccagtg ggacgtgcag
     3721 cagttcttgg cagagcaggg ccagctctgc tgtgtcatct ccagggtctc ccatcacctc
     3781 tgctctttgc catggcaggt ctgctgagac cccgcgggga cgggggcatg gtgctccctg
     3841 attggcctgt gaccaacctt ctggaaggct gctggcagtt ttccctgttt tccaccaccc
     3901 cactcttttt aataattgta tataactgta cttgttctac ttgcttgtct ttaaaacagg
     3961 ggcccccaca gttcactctc actgttagat tttgcctttt ccaggtatcc ccaacctgca
     4021 ataaactctt ccctcttcag aaaatcc
//
