LOCUS       NM_001195538            7979 bp    mRNA    linear   ROD 08-OCT-2024
DEFINITION  Mus musculus doublecortin-like kinase 1 (Dclk1), transcript variant
            5, mRNA.
ACCESSION   NM_001195538
VERSION     NM_001195538.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Modder,M., Coomans,C.P., Saaltink,D.J., Tersteeg,M.M.H.,
            Hoogduin,J., Scholten,L., Pronk,A.C.M., Lalai,R.A., Boelen,A.,
            Kalsbeek,A., Rensen,P.C.N., Vreugdenhil,E. and Kooijman,S.
  TITLE     Doublecortin-like knockdown in mice attenuates obesity by
            stimulating energy expenditure in adipose tissue
  JOURNAL   Sci Rep 14 (1), 19517 (2024)
   PUBMED   39174821
  REMARK    GeneRIF: Doublecortin-like knockdown in mice attenuates obesity by
            stimulating energy expenditure in adipose tissue.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 7979)
  AUTHORS   Ji,L., Yang,X., Jin,Y., Li,L., Yang,B., Zhu,W., Xu,M., Wang,Y.,
            Wu,G., Luo,W., Lee,K. and Liang,G.
  TITLE     Blockage of DCLK1 in cardiomyocytes suppresses myocardial
            inflammation and alleviates diabetic cardiomyopathy in
            streptozotocin-induced diabetic mice
  JOURNAL   Biochim Biophys Acta Mol Basis Dis 1870 (1), 166900 (2024)
   PUBMED   37778481
  REMARK    GeneRIF: Blockage of DCLK1 in cardiomyocytes suppresses myocardial
            inflammation and alleviates diabetic cardiomyopathy in
            streptozotocin-induced diabetic mice.
REFERENCE   4  (bases 1 to 7979)
  AUTHORS   Chen,X.Y., Kao,C., Peng,S.W., Chang,J.H., Lee,Y.L., Laiman,V.,
            Chung,K.F., Bhavsar,P.K., Heriyanto,D.S., Chuang,K.J. and
            Chuang,H.C.
  TITLE     Role of DCLK1/Hippo pathway in type II alveolar epithelial cells
            differentiation in acute respiratory distress syndrome
  JOURNAL   Mol Med 29 (1), 159 (2023)
   PUBMED   37996782
  REMARK    GeneRIF: Role of DCLK1/Hippo pathway in type II alveolar epithelial
            cells differentiation in acute respiratory distress syndrome.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7979)
  AUTHORS   Yang,B., Zhao,Y., Luo,W., Zhu,W., Jin,L., Wang,M., Ye,L., Wang,Y.
            and Liang,G.
  TITLE     Macrophage DCLK1 promotes obesity-induced cardiomyopathy via
            activating RIP2/TAK1 signaling pathway
  JOURNAL   Cell Death Dis 14 (7), 419 (2023)
   PUBMED   37443105
  REMARK    GeneRIF: Macrophage DCLK1 promotes obesity-induced cardiomyopathy
            via activating RIP2/TAK1 signaling pathway.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7979)
  AUTHORS   Burgess,H.A. and Reiner,O.
  TITLE     Cleavage of doublecortin-like kinase by calpain releases an active
            kinase fragment from a microtubule anchorage domain
  JOURNAL   J Biol Chem 276 (39), 36397-36403 (2001)
   PUBMED   11473121
  REMARK    GeneRIF: DCLK is a physiological substrate for the cysteine
            protease calpain. In neurons cleavage of DCLK by calpain represents
            a calcium responsive mechanism to regulate localization of the DCLK
            kinase domain
REFERENCE   7  (bases 1 to 7979)
  AUTHORS   Lin,P.T., Gleeson,J.G., Corbo,J.C., Flanagan,L. and Walsh,C.A.
  TITLE     DCAMKL1 encodes a protein kinase with homology to doublecortin that
            regulates microtubule polymerization
  JOURNAL   J Neurosci 20 (24), 9152-9161 (2000)
   PUBMED   11124993
REFERENCE   8  (bases 1 to 7979)
  AUTHORS   Burgess,H.A., Martinez,S. and Reiner,O.
  TITLE     KIAA0369, doublecortin-like kinase, is expressed during brain
            development
  JOURNAL   J Neurosci Res 58 (4), 567-575 (1999)
   PUBMED   10533048
REFERENCE   9  (bases 1 to 7979)
  AUTHORS   Sossey-Alaoui,K. and Srivastava,A.K.
  TITLE     DCAMKL1, a brain-specific transmembrane protein on 13q12.3 that is
            similar to doublecortin (DCX)
  JOURNAL   Genomics 56 (1), 121-126 (1999)
   PUBMED   10036192
REFERENCE   10 (bases 1 to 7979)
  AUTHORS   Omori,Y., Suzuki,M., Ozaki,K., Harada,Y., Nakamura,Y., Takahashi,E.
            and Fujiwara,T.
  TITLE     Expression and chromosomal localization of KIAA0369, a putative
            kinase structurally related to Doublecortin
  JOURNAL   J Hum Genet 43 (3), 169-177 (1998)
   PUBMED   9747029
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC111132.7, AC104099.5,
            AC115945.9 and BF467198.1.
            
            On Oct 28, 2017 this sequence version replaced NM_001195538.1.
            
            Summary: This gene encodes a member of the protein kinase
            superfamily and the doublecortin family. The protein encoded by
            this gene contains two N-terminal doublecortin domains, which bind
            microtubules and regulate microtubule polymerization, a C-terminal
            serine/threonine protein kinase domain, which shows substantial
            homology to Ca2+/calmodulin-dependent protein kinase, and a
            serine/proline-rich domain in between the doublecortin and the
            protein kinase domains, which mediates multiple protein-protein
            interactions. The microtubule-polymerizing activity of the encoded
            protein is independent of its protein kinase activity. The encoded
            protein is involved in several different cellular processes,
            including neuronal migration, retrograde transport, neuronal
            apoptosis and neurogenesis. This gene is up-regulated by
            brain-derived neurotrophic factor and associated with memory and
            general cognitive abilities. Multiple transcript variants generated
            by two alternative promoter usage and alternative splicing have
            been found, but the biological validity of some variants has not
            been determined. These variants encode different isoforms, which
            are differentially expressed and have different kinase activities.
            [provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (5, also known as DCLK-long-A,
            DCK-alpha2, KIAA0369-AL, DCAMKL1 or zyg8) is produced from the 5'
            promoter. It encodes isoform 5, which includes two doublecortin
            domains, a serine/proline-rich domain and a protein kinase domain.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC133685.1, SRR1660815.279438.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-388               AC111132.7         165449-165836
            389-784             AC111132.7         170216-170611
            785-1131            AC111132.7         178950-179296
            1132-1231           AC104099.5         22243-22342         c
            1232-1348           AC115945.9         108396-108512       c
            1349-1443           AC115945.9         96384-96478         c
            1444-1528           AC115945.9         81590-81674         c
            1529-1637           AC115945.9         79037-79145         c
            1638-1695           AC115945.9         72115-72172         c
            1696-1815           AC115945.9         71568-71687         c
            1816-1962           AC115945.9         69583-69729         c
            1963-2096           AC115945.9         59311-59444         c
            2097-2174           AC115945.9         57029-57106         c
            2175-2271           AC115945.9         56574-56670         c
            2272-2352           AC115945.9         52698-52778         c
            2353-2466           AC115945.9         42493-42606         c
            2467-7964           AC115945.9         20412-25909         c
            7965-7979           BF467198.1         1-15                c
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 26.53 cM"
     gene            1..7979
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /note="doublecortin-like kinase 1"
                     /db_xref="GeneID:13175"
                     /db_xref="MGI:MGI:1330861"
     exon            1..388
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            389..784
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            785..1131
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1132..1231
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1232..1348
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1443
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1528
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1637
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1638..1695
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1815
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1816..1962
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2096
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            2097..2174
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            2175..2271
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            2272..2352
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2466
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     exon            2467..7965
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7928..7933
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /note="hexamer: AATAAA"
     polyA_site      7958
                     /gene="Dclk1"
                     /gene_synonym="1700113D08Rik; 2810480F11Rik; Click-I;
                     Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369"
                     /note="major polyA site"
ORIGIN      
        1 ttgggagcgg aggggtgtcc aggcaatcca gattaccaag accattgcaa ccagaagcct
       61 ggctagagga gggaggaccc tggactcagc cacggtgggc gcgccctgcc aaggagggcg
      121 gagggcggag caggagaagg agggagaagg gagggcatgt gagcgagtga gacacaagaa
      181 aagcttgcgc gccaccgccc tcctcgggag agaggctgga gtgaggctga gcggagaacc
      241 gcatttcaat gaggaccagc tccagcgcat cagtgcacta gcggtcgcag cttccagacg
      301 ctcgtgctcc gcagccccag ccgcgcccag cccggcgagg acagctccag cagccggcca
      361 cagacaaccc agcctccacc cgcgaccggt tccataagca agccagccat gtcgttcggc
      421 agagatatgg agttggagca ttttgatgag cgggacaagg cgcagaggta cagcaggggg
      481 tcccgtgtga atggcctgcc cagccccaca cacagcgccc actgcagctt ctaccgcacc
      541 cgcaccctgc agacactcag ctccgagaag aaagccaaga aggttcgatt ctacagaaat
      601 ggtgaccgct acttcaaagg aattgtgtat gccatctccc cagaccgctt cagatctttc
      661 gaggccctgc tggctgattt gacccgaact ctctcggata atgtgaattt gccccagggg
      721 gtgagaacca tctacaccat cgatggactc aagaagatct ccagcctgga ccagctggtg
      781 gaaggtgaaa gctatgtctg cggctccatc gagcccttta agaagctgga gtacaccaag
      841 aatgtgaacc ccaactggtc agtgaacgtc aagaccacct cagcctcccg cgcagtgtct
      901 tctttggcca ctgccaaggg tgggccttcg gaggttcggg agaataagga tttcattcga
      961 cccaagctgg tcaccatcat cagaagtggg gtgaagccac ggaaggctgt cagaatcctg
     1021 ctgaacaaga agacggctca ctccttcgag caggttctca ctgacattac cgacgctatc
     1081 aagctggact ccggtgtggt gaagcgcctg tacactctgg atgggaagca ggtgatgtgc
     1141 cttcaggact tttttggtga cgatgacatt tttattgcat gtggaccaga gaagttccgt
     1201 taccaggatg atttcttgct agatgaaagt gaatgtcgag tggtgaaatc aacttcttac
     1261 accaaaatag catcagcgtc ccgcagaggc acaaccaaga gcccaggacc ttcccggaga
     1321 agcaagtccc cagcctccac cagctcagtt aatggaaccc ctggtagtca gctctctact
     1381 ccacgctcgg gcaagtcacc aagtccatca cccaccagcc caggaagcct gcggaagcag
     1441 aggatctctc agcatggcgg ctcctcgact tcactttcat ccactaaagt ttgcagctca
     1501 atggatgaga atgatggccc tggggaagaa gagtctgagg aaggcttcca gattcctgcc
     1561 acaataacag agagatacaa agtcgggaga acaataggag acggaaattt tgctgttgtc
     1621 aaggaatgta tagagaggtc gactgctcgg gagtatgccc tgaaaatcat caagaaaagc
     1681 aaatgccgag gcaaagagca catgatccag aacgaggtct ccatcctacg gagggtgaag
     1741 caccccaaca ttgtcctcct gattgaagag atggatgtgc cgactgaact gtatcttgta
     1801 atggaattag tgaagggtgg agaccttttc gatgccatca cctccactag caaatacaca
     1861 gagagagatg ccagcgggat gctgtacaac ctggccagcg ccatcaaata cctgcacagc
     1921 ctgaacatcg tccaccgtga catcaagcca gagaatctgc tggtgtatga gcaccaggat
     1981 ggcagtaagt cactcaagtt gggtgacttt ggcctggcca caattgtcga cggccccctg
     2041 tacacagtct gtggcacccc aacatatgtg gctccagaaa tcattgcaga gactggatat
     2101 ggcctcaagg tggacatctg ggcagctggc gtgatcactt atatcctgct gtgtggcttc
     2161 cctccgttcc gtggaagtgg ggatgaccag gaggtgcttt ttgaccagat cttgatgggc
     2221 caagtggact ttccatctcc gtattgggac aatgtgtcag attctgctaa ggagctcatc
     2281 aacatgatgc tgttggttaa cgtggaccag agattttcag ccgtgcaggt ccttgagcat
     2341 ccctgggtta atgatgatgg tctcccagaa aatgagcatc agctgtcagt agctggcaaa
     2401 atcaagaagc atttcaacac aggccccaag ccgagcagca ctgcagcagg agtttctgta
     2461 atagcaacca ccgctcttga taaggagagg caggttttcc gacgaagacg caaccaggat
     2521 gtgaggagcc ggtacaaggc gcagccagct ccaccggaat tgaactcgga atcggaggac
     2581 tactccccca gctcctctga gactgttcgc tcccccaatt cgccctttta ataagaccct
     2641 tttactcgaa gtcctagctt aaccctttaa gactctgaga tttccccctc cctccccaaa
     2701 cttctgtaaa acaggttcat ctgatctatc tagcactcag tgctgaccgg cagaacagaa
     2761 agtgtgtttt gggtaccttt gtagcaagtt ccctttactg aatgagaagg catgtggtgt
     2821 ttgtgacaat ggtaatttgc tgctaattgg ggtccttaaa gggttaaggc taatttgcga
     2881 tttttttttt aacatagaag taatgaatgt tttcatcagt caaactagga tctgcagtat
     2941 gtaacatagc acatgttagc cctctgagtg catagaattt tcttgagaat ccttgctggg
     3001 gaatttttca ggccttgaat atcttcacac atatttcttg attttactgc agaccaaggg
     3061 gcgtcataag acccaaaaaa tgtccacaca agaagagtat agaggatgac acactgccaa
     3121 tccttctctg tgggtttaga aaatgacaga ttttatagca tcagcagatg cacccatctt
     3181 ttcttaagca tcttaggaag aagggaagtc cctgtcccta cgtagtaagc tggttagctg
     3241 cagagacttc tctgctttga ttggagagaa ctgggtctgt ggcagcctaa ccctagagat
     3301 tgacagtgtc tctccctgtc tccatacaaa gatgtgctga agacgcccct gccacgctgc
     3361 ttgcccatta cctgctgcta gtgccaggca gaccctcggg gaaccaccta gccttggctt
     3421 gtgcttggtg agttgggatc tgtctgctca ggatcagaag agctcaagaa accactggta
     3481 aaaagagcaa taaattacca ggtgctctac agggctggga atccaaccag caagggaaag
     3541 caaggctctg tgttgttttt gttttgtttt gtttttctca gctgctttta cacaggaccg
     3601 tagccaccag tgtgaaacca acacagaatg gaaaagatct ggggcaagag gctagtgctt
     3661 tgcaaagagg cagaagggaa agttcagagg aaacacaatc caaaaggttc taaaacggga
     3721 ttcatagaaa caaaccgatt ccatggtaac tctaggtacc aggaagtcag taccagcaag
     3781 tctcccagaa gataccttgg cggctgaagc cgccactccc gttcatgatt ttctttactt
     3841 tagtgtggtc tccagtcctc caggggggat gtgttgcttt tctggtctgg ggccaaagcc
     3901 tgcctggggt tggtttttca ctcctgttac acagtccgtc tcccaggcct gagtttctta
     3961 gggagccgat tgtgtttcag aaggtgggct tgtgcttctc cgctacatcc gtgctccaga
     4021 ggattgcagg aggcaagacc caactcaaac attgccctaa tgttttctca gctttgactc
     4081 aggagagaac aattttacac tccaggacgg caacaggccc cttacttgct cattcttgct
     4141 ttgaatgtat tttaatgtct tgaaagttat gaacaatctt cccagagtca ggtctttatt
     4201 ttcgaactct acactgatct gcagctgctg tgtttataca tagtcatagg ttctgcacat
     4261 tcatagattt ttaaatgaca gaatcccaca tttcctgctc ttccacattt aaatataaca
     4321 tttatttagt ttttcttttc ctttttggga gtccagaaat gatacacata aaatcacaaa
     4381 atggtataaa tctgtacatt atttgaattg ggtgttggga ctaaggacag ggcacagacc
     4441 accagaaacg gtgtcagagt tcatatcggt aaaagactag aactggtccg aaatcactac
     4501 tttctgtgga acagaaaagc aaagcgctat tctaatcagc aagtgatggc atcactgagc
     4561 ttcaactcca cgtgtgtgac tataaaacac cctgtggcca agagcagatt gccacttccc
     4621 tttctctgaa ttactgcttt ccccggtcat taacgtatgg ccacagcatc ctggggtaca
     4681 ggcccacagt gcaaggctct ggaacatttc catcaacatt atctcatcag gttgcagaag
     4741 agcagctcag aattcagagc ccaggcagaa gggtgatcac agcacatccc tatagaattt
     4801 cagtgatcta cgctgaataa atagtggaat gtaacctgtc aagtagaaat attgagcaat
     4861 cagatggaat gcagtaattc agtaatcttg ccagcgtcaa gctaccaaga tcttagatgt
     4921 cagagttgta actcagaggg tgaacagaca agcacaggct gctgacttgg tgcatccaga
     4981 ccgctttgct tctacccagt acaaatatcc tcccttcaac attctcagaa tagctccagt
     5041 ttgaaataat taagtggtgg tgttctttga atttctataa ccaaatcgat cttatttttt
     5101 gtttggtgta tcatagaaca actatgtgcc atcatggctg tgtatcgctg taattatcca
     5161 ctgaaccatc atgactgttt ccatattttt gcatattgaa tataactctt atggtggtag
     5221 tctggtgatg ggaactgccc ttcctttact agcaagacca gtcttatggt attgaaagga
     5281 ttctttcagt ctgagagtag agtagttgaa cactccttca ttacttgctt cctgtaaatc
     5341 aattctgtag atgagaccca tttaatgaat acattttccc cccctccaca ttgtagaagg
     5401 aaccacttta tttgtcatga tcaataaatg tgtgaattgt tccaaaccat tagaaggaaa
     5461 aggtacggtc ttcagtccct ggaaatgttc tggctgtcgt cttcacctga ctcacacgtg
     5521 gtgtgtatta acttaagtcg aggggaaata agaataaaca gcggaaaaca cttgatgctt
     5581 tgcgttcgcc gagacttccc tgcatgcagt ctgctgggcc cccttatgag tggtggttaa
     5641 cagacctgtc acatagcgtt gagagagaac agtatatctg tgaatgaagg ctaaaattgc
     5701 aatcctttac cctttgaggc atatttcagt tgaaaaaaac aacaggaaag aaaaattgtc
     5761 tcagagggcc accgagctac cgaatcacca ggactcaagc ccgttaaatc atggttgttt
     5821 gctggccaaa ggcatgggct agacaactct atcccccgtg ctgaagtgca gttgtgctga
     5881 gagggaatcg tctccacatt actgcactgc ctctagtcct tctctgggta aagccctgtc
     5941 agtgtttcca tgataaactt ctattctgga aggtttttaa tttgaccata aaaatgtgcc
     6001 tcaggctgaa gtttgctagt cagggcctgt gctgaagagt gaggggggca catctcaccc
     6061 ttctccactc ccgaggaaaa gtcaacacag agacgtctgg tacgttgcct tcattgtgat
     6121 cccagagtct tggaatgcac caagatccaa gatggtaatt ttaacataat tattcaatta
     6181 ttcatttgga tatgttcttt tcaaattggc tcacctaaca catatcataa aataattttt
     6241 attattttaa cttgttaatt actatatgat tgtctctctg tgtctgtctg tctgtctctc
     6301 tctctctctt tctctgcccc ccccccccac acacacacac acttctccct ctgtccaaag
     6361 attttaagaa cttgaacttg atcagctcaa aggtccactt ttcccatgag ctacctgcta
     6421 cttccattgg aaattcgcac aaagaactct ctgacccagg ggagcacggg gttgtacgct
     6481 gtgtggaggg tcagatacat gcttcttttg gatatctggg tttccggggt tttaatcatc
     6541 agatttcatg aagccactct agtctgttgg gagaatagcg gtccaaagag cccccacaat
     6601 taacactttt gtcaactcag attcgaagcc agcgtaaaat aatacacttc ctactctcct
     6661 gtttaaaggc aaataaatca gagaaacttg aggacatatg aaacattgga tgactacaaa
     6721 cgtgctttac acttgggctc tgcggtgctt acacatggag caactaagaa ctggaaccct
     6781 gggacgtcag atggaatctt tagtttgtat gtaattacat acagatgggg gaggtgcttt
     6841 aagtggctct tttgtttttt aatcatctca aatatgcagc cagccagcca gccagtagca
     6901 ctaagggtca taacctgatg ggaaacagga aatctaaggt agaggttttt gagtgcctgg
     6961 gctcaggcat ttttgtttgt ttgtttctgt tgctggctct aacatggggg cctctgtggt
     7021 cagttttccg gtaagtgagc atctaactgg caggtgttgt gatcatgcag tttgcccaaa
     7081 gagacaaggg gtgtggattt attgctttct gtcaagaaaa ggcaatctca tacctgtaaa
     7141 tttcctcaac attttttttt ttggtcaatt aaactgagac ccttaatgtt actttaatgt
     7201 aaaattgtat agttttgact gtgacatact ttattaattt taagtaatta gtgcttaagc
     7261 cttcagaact gttggtatca agagtaggac tttaaagtgg tgttaactta acaatgctca
     7321 ttcattccaa ctcactcagc tgtagtcagg gacagagcag aatagctctc ttacatgccc
     7381 ctgacattaa atccttggtg tgttcaaaga ataaataaag agaaattgaa gctggttcaa
     7441 ggttagagtc atatccttga caacttttta aaaatttttt tattaggaaa ttaatattag
     7501 cctttttcat tctggctgaa agaaaattat tagaggatta cttgagtgtg aaattgaata
     7561 gtactttgcc cctgcatact aaagaggtgg gctggggact tgacacattt aacaagtttc
     7621 cctcaaggtt tcgatttgga gaaaacaaat tcaacatttc ctttggagag tctgcccttg
     7681 tcacttgctg catggatcgg atgacgtagg ttagtgtctg attttggaat cccaatgaaa
     7741 taactttcaa acaatttgtc cccatgatta aaggtggaat ggtatccaat ttccccccta
     7801 atccttcagt ttaagctcat aaattgtagg tgaatgtgga atgaaatcat ctgtgatata
     7861 ttatgttcat ttaccaaacg agctcttttg atgttgcctg tttttatgta aaacatgttc
     7921 ttaaactaat aaagtcatat ctcttggtgt acaaattatt cacaaaaaaa aaaaaaaaa
//
