LOCUS       NM_177577               6894 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus doublecortin domain containing 2a (Dcdc2a), transcript
            variant 1, mRNA.
ACCESSION   NM_177577
VERSION     NM_177577.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Liu,Q.Q., Chen,J., Ma,T., Huang,W. and Lu,C.H.
  TITLE     DCDC2 inhibits hepatic stellate cell activation and ameliorates
            CCl4-induced liver fibrosis by suppressing Wnt/beta-catenin
            signaling
  JOURNAL   Sci Rep 14 (1), 9425 (2024)
   PUBMED   38658618
  REMARK    GeneRIF: DCDC2 inhibits hepatic stellate cell activation and
            ameliorates CCl4-induced liver fibrosis by suppressing
            Wnt/beta-catenin signaling.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Hauser,B.R., Aure,M.H., Kelly,M.C., Hoffman,M.P. and Chibly,A.M.
  CONSRTM   Genomics and Computational Biology Core
  TITLE     Generation of a Single-Cell RNAseq Atlas of Murine Salivary Gland
            Development
  JOURNAL   iScience 23 (12), 101838 (2020)
   PUBMED   33305192
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6894)
  AUTHORS   Martynova,E., Zhao,Y., Xie,Q., Zheng,D. and Cvekl,A.
  TITLE     Transcriptomic analysis and novel insights into lens fibre cell
            differentiation regulated by Gata3
  JOURNAL   Open Biol 9 (12), 190220 (2019)
   PUBMED   31847788
REFERENCE   4  (bases 1 to 6894)
  AUTHORS   Rendall,A.R., Perrino,P.A., LoTurco,J.J. and Fitch,R.H.
  TITLE     Evaluation of visual motion perception ability in mice with
            knockout of the dyslexia candidate susceptibility gene Dcdc2
  JOURNAL   Genes Brain Behav 18 (5), e12450 (2019)
   PUBMED   29232042
  REMARK    GeneRIF: Dcdc2 KOs displayed deficits on the Pairwise
            Discrimination task specifically as motion was added to visual
            stimuli.
REFERENCE   5  (bases 1 to 6894)
  AUTHORS   Scavuzzo,M.A., Hill,M.C., Chmielowiec,J., Yang,D., Teaw,J.,
            Sheng,K., Kong,Y., Bettini,M., Zong,C., Martin,J.F. and Borowiak,M.
  TITLE     Endocrine lineage biases arise in temporally distinct endocrine
            progenitors during pancreatic morphogenesis
  JOURNAL   Nat Commun 9 (1), 3356 (2018)
   PUBMED   30135482
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6894)
  AUTHORS   Gabel,L.A., Marin,I., LoTurco,J.J., Che,A., Murphy,C., Manglani,M.
            and Kass,S.
  TITLE     Mutation of the dyslexia-associated gene Dcdc2 impairs LTM and
            visuo-spatial performance in mice
  JOURNAL   Genes Brain Behav 10 (8), 868-875 (2011)
   PUBMED   21883923
  REMARK    GeneRIF: heterozygous and homozygous mutations of Dcdc2 result in
            persistent visuo-spatial memory deficits, as well as visual
            discrimination and long-term memory deficits
REFERENCE   7  (bases 1 to 6894)
  AUTHORS   Wang,Y., Yin,X., Rosen,G., Gabel,L., Guadiana,S.M., Sarkisian,M.R.,
            Galaburda,A.M. and Loturco,J.J.
  TITLE     Dcdc2 knockout mice display exacerbated developmental disruptions
            following knockdown of doublecortin
  JOURNAL   Neuroscience 190, 398-408 (2011)
   PUBMED   21689730
  REMARK    GeneRIF: These results indicate that Dcdc2 is not required for
            neurogenesis, neuronal migration or differentiation in mice, but
            may have partial functional redundancy with doublecortin.
REFERENCE   8  (bases 1 to 6894)
  AUTHORS   Dijkmans,T.F., van Hooijdonk,L.W., Fitzsimons,C.P. and
            Vreugdenhil,E.
  TITLE     The doublecortin gene family and disorders of neuronal structure
  JOURNAL   Cent Nerv Syst Agents Med Chem 10 (1), 32-46 (2010)
   PUBMED   20236041
  REMARK    Review article
REFERENCE   9  (bases 1 to 6894)
  AUTHORS   Reiner,O., Coquelle,F.M., Peter,B., Levy,T., Kaplan,A., Sapir,T.,
            Orr,I., Barkai,N., Eichele,G. and Bergmann,S.
  TITLE     The evolving doublecortin (DCX) superfamily
  JOURNAL   BMC Genomics 7, 188 (2006)
   PUBMED   16869982
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 6894)
  AUTHORS   Coquelle,F.M., Levy,T., Bergmann,S., Wolf,S.G., Bar-El,D.,
            Sapir,T., Brody,Y., Orr,I., Barkai,N., Eichele,G. and Reiner,O.
  TITLE     Common and divergent roles for members of the mouse DCX superfamily
  JOURNAL   Cell Cycle 5 (9), 976-983 (2006)
   PUBMED   16628014
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC045136.1, AK220370.1,
            AL589735.12 and AW492955.1.
            
            On Feb 14, 2008 this sequence version replaced NM_177577.2.
            
            Summary: This gene encodes a member of the doublecortin family. The
            protein encoded by this gene contains two doublecortin domains. The
            doublecortin domain has been demonstrated to bind tubulin and
            enhance microtubule polymerization. Alternatively spliced
            transcript variants encoding distinct isoforms have been found for
            this gene. [provided by RefSeq, Sep 2010].
            
            Transcript Variant: This variant (1) is the longer transcript and
            encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK220370.1, SRR7345562.241254.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849384
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-303               BC045136.1         1-303
            304-1307            AK220370.1         77-1080
            1308-1548           AL589735.12        29870-30110         c
            1549-1661           AK220370.1         1322-1434
            1662-1662           AL589735.12        26810-26810         c
            1663-1736           AK220370.1         1436-1509
            1737-6580           AL589735.12        21892-26735         c
            6581-6894           AW492955.1         1-314               c
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 10.89 cM"
     gene            1..6894
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /note="doublecortin domain containing 2a"
                     /db_xref="GeneID:195208"
                     /db_xref="MGI:MGI:2652818"
     exon            1..560
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     CDS             268..1695
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     doublecortin domain-containing protein 2"
                     /codon_start=1
                     /product="doublecortin domain-containing protein 2 isoform
                     1"
                     /protein_id="NP_808245.2"
                     /db_xref="CCDS:CCDS26386.2"
                     /db_xref="GeneID:195208"
                     /db_xref="MGI:MGI:2652818"
                     /translation="MNGPSSRSSHLSQPVVKSVLVYRNGDPFFAGRRVVIHEKKVSSF
                     DVFLKEVTGGVQAPFGAVRNIYTPRTGHRIRKLDQIESGGNYVAGGPEAFKKLNYLDI
                     GEIKKRPMEAVNTEVKPVIHSRINVSARFRKSLHEPCTIFLIANGDLISPASRLLIPK
                     KALNQWDHVLQMVTEKITLRSGAVHRLYTLEGKLVESGAELENGQFYVAVGRDKFKRL
                     PYSELLFDKSAMRRPYGQKASSLPPMVGSRKSKGSGNYRQSKSTIGSSDNSSPQPLKR
                     KGKKDSNSEKPTKVKQSVKSKTSHQAIPDNGEGIFKAGAERSETRGAAEVQEDEDTQV
                     EVPVDQRPAEIVDEEEDGEKTSKDANQKEDFSAMNGETEDRGGSKAAGTSEQEEGIPD
                     HGEKKASPSRVNGGTDEENGEELDQVAEELQSTEDEKGKAEGDNSGQDEAGLDAQRPP
                     RPEVTVTSPQENEENEANKASSAVA"
     exon            561..615
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     exon            616..692
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     exon            693..824
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     exon            825..971
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1026
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1027..1186
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1287
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1288..1590
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..6883
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2121..2126
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /note="hexamer: AATAAA"
     polyA_site      2148
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /note="major polyA site"
     regulatory      6859..6864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
                     /note="hexamer: AATAAA"
     polyA_site      6882
                     /gene="Dcdc2a"
                     /gene_synonym="AW492955; Dcdc2; RU2"
ORIGIN      
        1 ccggctcgcg caggggttta aagcccgagc ggcgtgggtt cccagactgc acacctggat
       61 gcttcggggc gggggagaca ggtgtgcgag cctgcgagcg ctgattggcc gtccgtgccg
      121 cgccttcctt gaattgctac ctgctagggc tggccccgca gcccttcctc acctgccgct
      181 agctttcctt gtccctgcag gaggcggtcc acgagctgct gttgcaggcc ggtggagtgc
      241 ttgcgggctc ggcgtcccgc ggagaagatg aacggtccca gctccaggtc cagccacctg
      301 tcgcagccgg tggtgaagag tgtgctggtg taccgcaatg gggacccctt tttcgccggg
      361 cggcgtgtcg tcattcacga gaagaaggtg tccagcttcg atgtcttcct caaagaagtg
      421 actggcggtg tgcaggcgcc ttttggggcg gttaggaaca tctacacccc gaggacgggc
      481 caccgcatcc gaaaactgga ccagatagag agcgggggca actatgtggc tgggggcccg
      541 gaagcgttca agaaactcaa ttacttggac ataggcgaaa tcaagaaaag accaatggaa
      601 gctgtgaata ccgaggtgaa acctgtcatt cacagcagga tcaacgtgtc cgcccgcttc
      661 aggaagtcgc tgcacgagcc ctgcaccatt ttcttgattg caaatggaga cctcataagc
      721 ccggcttctc gcctcctcat ccctaaaaaa gctttaaatc agtgggatca tgtactacaa
      781 atggtcacag aaaagataac tctccggagt ggggctgtcc acaggctata tacgttagaa
      841 ggaaagctgg ttgagagtgg agcagaactg gagaatgggc agttctatgt ggccgtcggc
      901 agagataagt ttaagagact accgtacagt gagttacttt ttgacaagtc agcaatgaga
      961 aggccttacg gtcagaaagc gtcttcacta cctcccatgg ttggatccag gaagtctaaa
     1021 gggagcggaa attatcggca atccaagtca accatcgggt ctagtgataa ctcatctcct
     1081 cagcctctga aaaggaaagg gaagaaagac tcaaactcag aaaaacccac gaaagtgaaa
     1141 cagagtgtga agtcgaagac ttcccaccaa gcaatcccag acaatggtga aggcattttc
     1201 aaagctggag cagagagatc tgaaacaagg ggggcagcag aagttcaaga agatgaagac
     1261 acccaggttg aggttccagt ggaccagagg ccagcagaaa tagttgatga ggaagaagat
     1321 ggtgagaaaa caagcaaaga tgccaaccag aaggaagact tttcagccat gaatggtgag
     1381 actgaagaca gaggggggag caaagcggct ggtacctctg agcaggagga gggaatccca
     1441 gaccacgggg agaagaaggc aagcccctct cgggtaaatg gtggcacgga tgaagagaat
     1501 ggggaggaac tggaccaggt tgccgaggag ctccaatcaa cagaggacga gaaaggaaag
     1561 gctgaaggag acaacagtgg gcaagatgag gctggtttgg atgctcaaag accaccaaga
     1621 ccagaagtga cagtcaccag ccctcaagag aatgaagaga acgaagcaaa caaggcctct
     1681 tctgctgtgg catagatggt ttaaagcaga gaatatgagc gatgtgagag gatgcagagc
     1741 tataatactt gaataatgag cacagcatct cttcatgtag aatgtggcaa tgtggtggag
     1801 gccctaccta ttgcatttta aaagtagtag ctacatgaaa ggaagggtta ggacatttgt
     1861 aactcaataa cttaagctta tgacaggtgt ttaaaaaatt cctttagaag accaaaagag
     1921 agacagggga tttgcacctt aagacagcag ctggctacct gtgtttgtaa aggggaaaga
     1981 catgggctag ctcccattag atactttatt atagacttgg aagcaagaat actggcagct
     2041 tgttctaaag catttatcta ggaatgtata gagtttttaa gaattggtaa taggagaaac
     2101 gtgtattatg ttcttgaagc aataaactgt tgattgaaca aattgcaatt tagtttcaga
     2161 gataatttgg agatggcaat agatctgaac actctaaagc tggtgacatt cttttctttc
     2221 tccagccaag gtccaccttg ggcaaagtca aaacacagcc caaggtgtca cctggtcaga
     2281 ggaaaagctt ctagagcgcc tggtgattca tgtcaggtgt gttggatgtg actggcacac
     2341 tcccaggcag gaaccagaaa gtatgaccta gacatcctgt gtttttttca ctgctctgtt
     2401 ttcactgtgt ctgtacaagg aacaaagcct ttccttctcc tatatgctgt tgggaaaccc
     2461 aatattggtt aatattaaat tcttttcagg attttaaaaa tgaacttttt attaaacaca
     2521 aaggaacaaa aaaggaagca aaagcagaac aatttatggc gatccaattg cttagctact
     2581 tgcactttgc gaaatgactg tcatggctgt ttagattatg tccatctgtc catctactca
     2641 tccatctgtc tgtctgtcca tccatccgca catgtatctc ctaagctttt agactttcaa
     2701 gcagactttt ggactatttt agcctttcaa gcagattctc tctctctctc tctctctctc
     2761 tctctatata tatatatata tatatatata tatatatgta tatgtatata tatatatata
     2821 tatatacata tatatatata tatatccatc tgatggaaat tgttgaaaaa atttgaaata
     2881 tttccatggc tcttgatttc agtttgtgga tagataaact aatatgcatt caccttgtga
     2941 ggttcagagg aaggaaaaga taattatatt agtttgacga tcatagtaca gatgatgtct
     3001 ctccccctaa atacagaaat aatatgtttt tctcatttta aggaatatat ttttactcag
     3061 aaaatgaaaa ctggaagatt ttagattgta gtgcctttgt aagattcatt actattgtaa
     3121 gtctattttc ccctctagat tttgcaacca accatctggg aacaaaatag ttgggagtta
     3181 tatatactaa agtaatgtag aaaatagatg tatttttccc caaagctatt ttgattcttc
     3241 cataaataga aaatatgaac agagcttatg acatcacagt gtttgaggga gatcgtgaca
     3301 cgaggcctgg gaatccggga gaatgggctt taactggggc acagtctggg cggtgctttt
     3361 tgtgcacaca cttactttta acacgcaggg actgccagaa gccatcttgc actgttagga
     3421 aaacagaggt gctctccttc tgatgtgact ttccagcatc tggggttgaa gattcaaata
     3481 tcttacaaaa gttgaatata agaagtttta actaagcctc aaactgattc tctttctcac
     3541 catggagggt gtgtctcctt aaggaagcac cactctttgc cccccaccct tcacttcact
     3601 gtggcacttg ggttttgtgg gttctcttgc cagctgtctt aatgaggaac aacactgata
     3661 cttcacataa aaaacaaact ctgagatctt aacatttcaa gaaggttata ggcatagtaa
     3721 caaccataga atgttgaaat acgtgggggt atcatcattt tctatgcttg catgggaccc
     3781 aaatatgtga ctcaaatcaa aaccatctcc atatgttgtc tagccaaaca tgtaatcctt
     3841 tattggggac taaaactata ggcataactg tgaaggtgac ctttatagaa gttgaagttc
     3901 agttcacctc tcaaaagtgt tggtctcccc tgtggaaatt gggtagcaat gacagattag
     3961 ggccacatgt aagtgagact tactgacaaa tttgggaatt acacatagag cacacggatg
     4021 agggcttggt gaagggttat gtgtcattca taatgaaggc tcctggatat atacctgcaa
     4081 tgccttgctt tgaagtgtca gctctttgca atttcttagt tttaatttta tgtcactaat
     4141 gccttagcca catttcaatt ttaagggcac tcatgatcat ctgctgatgg aattggaaca
     4201 ctggtagttt tgcacttgac tagagtgact ttgactaaag tcgccgtcag atgagaggga
     4261 tgtgctgcat gctttggcct gtgttgaagc cacagttgcc tgcccatcag cttcctgtta
     4321 ctatgactac agggcagccc ccccccaact ctctaggttt tcctggggcc ctggagagac
     4381 ttgttattta agacattttt cttgcattct atttttttaa tgtcggaatg ccagactcat
     4441 aggaagagca tgataaggtc atttatccag ttcaagtcta aagggtgaat tcttgtgtgg
     4501 tgtctaatga cataccacat cagaagtgat gctggtatgt ttcctagggt agccctaggg
     4561 tagggctaaa ccactagaaa cctcagagtt ttctttaggg ggatggcatg atggttcagc
     4621 agttaaagtg cctgctacca agcctgatgt cctatactgt gacatgtata taccaaagtc
     4681 atccccaaca cacacacaca cacacacaca cacacacaca cacacacaca cacaaaacac
     4741 acgcgcatac acacagagtt aaataagtaa gtataaatgc gttgttttct ttaggcaacc
     4801 tgccctatgc acagaggatg agtttcactg gtctagagaa gataatgtgc ttaaagctgt
     4861 ggctgctgtc gggcacagca gctcatacct gtaatcccac aactggggag caggaggtgg
     4921 atcaagaatt aaaggttgaa gcacacttgg ctacatagtg tgcttgaggc taacctcaat
     4981 atgagatccc cgtttaaaaa acaaaacaaa acaaaactga ccaacctagc aacctaccta
     5041 cctacccacc ccaagctaaa gctaaacttg ccaccacaga aaaataggaa ggtatactca
     5101 tcataggtaa ccagaaggca tactgtgatt ctaacatgtc ctgtaaggaa tccactctac
     5161 acttttactg ttgcggttaa agtagcaaaa gcaaagctaa agagtaagtt catctgggag
     5221 gagaaaactc cgcatctgag ctggtgccaa tcagaagaaa acgtcacacc cagtgcacgt
     5281 atctaccagc acctccaagg gtagctcata tactctcagg cattaagaag gacctcctta
     5341 ctgctctgca cagagtaagg ctctcgcatg gggagaagca gagctaacag tgtgagagca
     5401 aatagtaaga gactactgtt atatcagcgt taatattgca ttactgggac caagagatac
     5461 aatatatttc cccaataaga cagttaccag catcacttaa agtaagcatg gtggcatatg
     5521 actactaccc cagtgctcta gaggtagagg cagaggggtc agaagttcaa ggtcatttcc
     5581 agctgcccag tgaggtccag accagtgtga gctataagag acccatttaa aacaaaacaa
     5641 aacaaaacaa aacaaaacaa aacaaaacaa aacaaaacag aggaagagat gctaaaatta
     5701 gttttagtta cattttaaaa aatgagagat gtgcaccttg ttgcctgaac tgatgacagg
     5761 caacagtctc tgcggcagcc taaagtatca tgtggacttt tggggggcag agggagcagg
     5821 gaggaaacct gggacgactc cttgtatcca gaaaccaggt acgacacaga gtgactctaa
     5881 tacattgtca ccctgggcag gaaccagttt ctgaaggtta gcctgctttt ctaaccagag
     5941 acacttggag ttcgttttca ctgattggca tgttcataca cataaggcat ttgcttgaca
     6001 cataccctgg tatagagaca ggtctagctt ttgcgttgta tattttgtcc catgatgttt
     6061 ggaagtcaga aggggaatga aacagtgtcc ccatacattg agcaagaaac tcagcattgt
     6121 tgtaaaaaga gtcaacacct gaaagagatg tgcaattagg aaagaattaa aaacaataat
     6181 taaaaaaagc acttataaaa tctgagggga ccagtggagc tctgtccagg agccaatggc
     6241 tgcctagctt cgacctgaaa atgtgaccaa atgaattcca cgtagtctta ccccccttga
     6301 agtttaactg actgttttag ttactgtcaa aaaggagggg tgaggttgtg gtcagcataa
     6361 gaggattgca ggaagctctg ttgctttcat gttctctttg cagtcaacat tgctaacgcg
     6421 tacatagtaa tggctaattt ttcatgcact atatagagat taaattaagc tcatagtttc
     6481 agtagcattt gttatattta gaatgccttt atgtaaggaa aagtatgcat tttgatcaaa
     6541 ttatggtagg tggcttttat ttctgtatcg gagcacattc taaaatgctc gtacacctgt
     6601 ccggtcctgt gggtagatga gtgctttatg tagttaacat ccatgttcag cattccatgt
     6661 gatactgtgt tgttaatatt tcatgtgtga atcctttttg tagatttttc atgaacagga
     6721 gagagagaga gataggggtc tagactcacg tagacattca gctcatttct ttattgtacg
     6781 agatattttt agagaaccac aggttccgaa gtttttgtga tgctcatggc gtgtattttg
     6841 tatacagaca accacagaaa taaatgtatc attttacaaa gagaaaaaaa aaaa
//
