LOCUS       NM_001356484            6481 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus discoidin, CUB and LCCL domain containing 2 (Dcbld2),
            transcript variant 2, mRNA.
ACCESSION   NM_001356484 XM_006522622
VERSION     NM_001356484.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6481)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6481)
  AUTHORS   Wang,J., Zhang,H., Chen,L., Fu,K., Yan,Y. and Liu,Z.
  TITLE     CircDCBLD2 alleviates liver fibrosis by regulating ferroptosis via
            facilitating STUB1-mediated PARK7 ubiquitination degradation
  JOURNAL   J Gastroenterol 59 (3), 229-249 (2024)
   PUBMED   38310161
  REMARK    GeneRIF: CircDCBLD2 alleviates liver fibrosis by regulating
            ferroptosis via facilitating STUB1-mediated PARK7 ubiquitination
            degradation.
REFERENCE   3  (bases 1 to 6481)
  AUTHORS   Wang,S., Liu,X., Meng,Z., Feng,Q., Lin,Y., Niu,H., Yu,C., Zong,Y.,
            Guo,L., Yang,W., Ma,Y., Zhang,W., Li,C., Yang,Y., Wang,W., Gao,X.,
            Hu,Y., Liu,C. and Nie,L.
  TITLE     DCBLD2 regulates vascular hyperplasia by modulating the platelet
            derived growth factor receptor-beta endocytosis through Caveolin-1
            in vascular smooth muscle cells
  JOURNAL   FASEB J 36 (9), e22488 (2022)
   PUBMED   35929441
  REMARK    GeneRIF: DCBLD2 regulates vascular hyperplasia by modulating the
            platelet derived growth factor receptor-beta endocytosis through
            Caveolin-1 in vascular smooth muscle cells.
            Erratum:[FASEB J. 2023 Sep;37(9):e23150. doi:
            10.1096/fj.202301586.. PMID: 37594909]
REFERENCE   4  (bases 1 to 6481)
  AUTHORS   Hung,C.H., Lee,T.L., Huang,A.Y., Yang,K.C., Shyu,Y.C., Wen,S.C.,
            Lu,M.J., Yuan,S. and Shen,C.J.
  TITLE     A Positive Regulatory Feedback Loop between EKLF/KLF1 and TAL1/SCL
            Sustaining the Erythropoiesis
  JOURNAL   Int J Mol Sci 22 (15), 8024 (2021)
   PUBMED   34360789
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6481)
  AUTHORS   Coppo,R., Orso,F., Virga,F., Dalmasso,A., Baruffaldi,D., Nie,L.,
            Clapero,F., Dettori,D., Quirico,L., Grassi,E., Defilippi,P.,
            Provero,P., Valdembri,D., Serini,G., Sadeghi,M.M., Mazzone,M. and
            Taverna,D.
  TITLE     ESDN inhibits melanoma progression by blocking E-selectin
            expression in endothelial cells via STAT3
  JOURNAL   Cancer Lett 510, 13-23 (2021)
   PUBMED   33862151
REFERENCE   6  (bases 1 to 6481)
  AUTHORS   Li,X., Jung,J.J., Nie,L., Razavian,M., Zhang,J., Samuel,V. and
            Sadeghi,M.M.
  TITLE     The neuropilin-like protein ESDN regulates insulin signaling and
            sensitivity
  JOURNAL   Am J Physiol Heart Circ Physiol 310 (9), H1184-H1193 (2016)
   PUBMED   26921437
  REMARK    GeneRIF: ESDN regulates insulin signaling and subsequently a wide
            array of insulin effects in cultured cells and in vivo through
            modulation of IR interaction with APS and Grb10.
REFERENCE   7  (bases 1 to 6481)
  AUTHORS   Nie,L., Guo,X., Esmailzadeh,L., Zhang,J., Asadi,A., Collinge,M.,
            Li,X., Kim,J.D., Woolls,M., Jin,S.W., Dubrac,A., Eichmann,A.,
            Simons,M., Bender,J.R. and Sadeghi,M.M.
  TITLE     Transmembrane protein ESDN promotes endothelial VEGF signaling and
            regulates angiogenesis
  JOURNAL   J Clin Invest 123 (12), 5082-5097 (2013)
   PUBMED   24177422
  REMARK    GeneRIF: Deletion of Esdn in the mouse interfered with adult and
            developmental angiogenesis.
REFERENCE   8  (bases 1 to 6481)
  AUTHORS   Boyle,M.P., Bernard,A., Thompson,C.L., Ng,L., Boe,A., Mortrud,M.,
            Hawrylycz,M.J., Jones,A.R., Hevner,R.F. and Lein,E.S.
  TITLE     Cell-type-specific consequences of Reelin deficiency in the mouse
            neocortex, hippocampus, and amygdala
  JOURNAL   J Comp Neurol 519 (11), 2061-2089 (2011)
   PUBMED   21491433
REFERENCE   9  (bases 1 to 6481)
  AUTHORS   Yoshida,Y., Han,B., Mendelsohn,M. and Jessell,T.M.
  TITLE     PlexinA1 signaling directs the segregation of proprioceptive
            sensory axons in the developing spinal cord
  JOURNAL   Neuron 52 (5), 775-788 (2006)
   PUBMED   17145500
REFERENCE   10 (bases 1 to 6481)
  AUTHORS   Kobuke,K., Furukawa,Y., Sugai,M., Tanigaki,K., Ohashi,N.,
            Matsumori,A., Sasayama,S., Honjo,T. and Tashiro,K.
  TITLE     ESDN, a novel neuropilin-like membrane protein cloned from vascular
            cells with the longest secretory signal sequence among eukaryotes,
            is up-regulated after vascular injury
  JOURNAL   J Biol Chem 276 (36), 34105-34114 (2001)
   PUBMED   11447234
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT027564.7.
            
            On Oct 5, 2017 this sequence version replaced XM_006522622.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066097.1, SRR17253012.8125783.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-417               CT027564.7         87897-88313
            418-645             CT027564.7         103923-104150
            646-783             CT027564.7         112688-112825
            784-835             CT027564.7         124703-124754
            836-908             CT027564.7         127814-127886
            909-1042            CT027564.7         128178-128311
            1043-1083           CT027564.7         129161-129201
            1084-1299           CT027564.7         130086-130301
            1300-1424           CT027564.7         131023-131147
            1425-1575           CT027564.7         134488-134638
            1576-1650           CT027564.7         135439-135513
            1651-1776           CT027564.7         135645-135770
            1777-1870           CT027564.7         140317-140410
            1871-1920           CT027564.7         142421-142470
            1921-2058           CT027564.7         143552-143689
            2059-6481           CT027564.7         144685-149107
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 34.52 cM"
     gene            1..6481
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /note="discoidin, CUB and LCCL domain containing 2"
                     /db_xref="GeneID:73379"
                     /db_xref="MGI:MGI:1920629"
     exon            1..417
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..2525
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; discoidin, CUB and LCCL domain-containing
                     protein 2; endothelial and smooth muscle cell-derived
                     neuropilin-like protein"
                     /codon_start=1
                     /product="discoidin, CUB and LCCL domain-containing
                     protein 2 isoform 2 precursor"
                     /protein_id="NP_001343413.1"
                     /db_xref="GeneID:73379"
                     /db_xref="MGI:MGI:1920629"
                     /translation="MASRAPLRAARSPQGPGGPAAPAATGRAALPSAGCCPLPPGRNS
                     SSRPRLLLLLLLLLQDAGGQQGDGCGHTVLGPESGTLTSINYPHTYPNSTVCEWEIRV
                     RTGERIRIKFGDFDIEDSDYCHLNYLKIFNGIGVSRTEIGKYCGLGLQMNQSIESKGS
                     EVTVLFMSGTHAAGRGFLASYSVIDKEDLITCLDTVSNFLEPEFSKYCPAGCLLPFAE
                     ISGTIPHGYRDSSPLCMAGIHAGVVSNVLGGQISIVISKGTPYYESSLANNVTSTVGY
                     LSASLFTFKTSGCYGTLGMESGVIADPQITASSALEWTDHMGQENSWTAEKARLRKPG
                     PPWAAFATDEHQWLQIDLNKEKKITGIVTTGSTMIEHSYYVSAYRVLYSDDGQRWTVY
                     REPGVDQDKIFQGNKDYHKDVRNNFLPPIIARFIRVNPVQWQQKIAMKVELLGCQFTL
                     KGRLPKLTPPPRNGNNLRNTTARPKLGRAPKFTQVLQPRSRNELPVQPAETTTTPDIK
                     NTTVTPSVTKDVALAAVLVPVLVMALTTLILILVCAWHWRNRKKKTEGAYDLPHWDRA
                     GWWKGMKQLLPAKSVDHEETPVRYSTSEVSHLSAREVTTVLQADSAEYAQPLVGGIVG
                     TLHQRSTFKPEEGKEAGYADLDPYNSPMQEVYHAYAEPLPVTGPEYATPIVMDMSGHP
                     TASVGLPSTSTFKTAGTQPHALVGTYNTLLSRTDSCSSGQAQYDTPKGGKSAATPEEL
                     VYQVPQSTQELSGAGRDEKFDAFKEIL"
     exon            418..645
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            646..783
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            784..835
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            836..908
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            909..1042
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1083
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1084..1299
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1424
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1575
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1650
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1776
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1870
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1871..1920
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2058
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     exon            2059..6481
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2769..2774
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /note="hexamer: AATACA"
     polyA_site      2808
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /note="major polyA site"
     regulatory      5781..5786
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /note="hexamer: AATAAA"
     polyA_site      5810
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
     regulatory      5824..5829
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /note="hexamer: AATAAA"
     polyA_site      5846
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /note="major polyA site"
     regulatory      6456..6461
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
                     /note="hexamer: AATAAA"
     polyA_site      6481
                     /gene="Dcbld2"
                     /gene_synonym="1700055P21Rik; CLCP1; Esdn"
ORIGIN      
        1 gcgggagcgc gttccagctc gggccccggt gctatcctcc gatccgccgc gctccggcct
       61 ggggtctcgg ggcgggaagc tcggtgaagc gggcgcgggg agcagggcgg aggggacgag
      121 gcgcgctgcg ctgccgcccg gtcctcaccg ggtgctgcgg gccccgggcg cgcggggctc
      181 cgcggcgcgc gcactatgcg ggcggattgc cggccgccgc gatggcgagc cgggcgccgc
      241 tgagagccgc gcgcagcccc cagggtcccg gaggcccggc cgcgcccgcc gccaccggcc
      301 gggccgcgct gcccagcgcc ggctgctgtc ccctccctcc tggccgcaac tcctcctcca
      361 ggcctcgact gctccttctg ctgctcctac tgctccagga cgctggaggc cagcaaggtg
      421 atggatgtgg acacactgta ctaggccctg agagtggaac ccttacatcc atcaactacc
      481 cacataccta tcctaacagc actgtgtgtg aatgggagat tcgagtcagg acgggagaaa
      541 ggattcgcat caaattcggt gactttgaca ttgaagattc tgattattgt caccttaatt
      601 acctgaaaat ctttaatgga attggagtca gcagaacgga aataggcaaa tactgtggtc
      661 tgggtttaca aatgaatcag tcaattgagt ccaaaggcag tgaagtcaca gtgctgttca
      721 tgagtggaac ccatgctgct gggcgaggat ttttggcttc atactcagtt atagataaag
      781 aagatttaat cacttgtttg gatactgtat ctaatttttt ggaaccagag ttcagtaagt
      841 actgcccagc tggctgtctt ttgccttttg ctgaaatatc tggaacaatt cctcatggat
      901 acagagattc ttcaccattg tgtatggctg gaatccatgc aggagtagtg tcaaacgtgc
      961 tgggtggcca aatcagcatt gtgattagca aagggacccc atattatgaa agctctttgg
     1021 ccaacaatgt cacttccacg gtgggatact tatctgcaag tctgtttaca tttaagacaa
     1081 gtggttgcta tgggactctg gggatggagt ctggtgtgat tgccgatccc cagataacag
     1141 catcgtctgc actggagtgg actgaccaca tggggcagga gaacagctgg acagcggaga
     1201 aggccaggct gagaaaaccc gggcctccct gggctgcttt tgccactgat gagcatcagt
     1261 ggctgcagat agaccttaac aaggagaaga agataacagg tatcgtaacc actgggtcta
     1321 ccatgataga acacagttac tatgtgtctg cctacagagt cctgtacagt gacgatgggc
     1381 agagatggac tgtgtacaga gaacctggtg tggaccagga caagatattt caaggaaaca
     1441 aagattatca caaggatgtt cgtaataact ttttgccacc aattattgca cgtttcatta
     1501 gagtgaaccc tgtccagtgg caacagaaaa ttgccatgaa agtggaactg ctcggatgtc
     1561 agtttactct caaaggtcgc cttccaaagc ttactccacc tcctcggaac ggcaataacc
     1621 tcagaaatac tacagctcgt cccaaactag gtcgtgcccc taaatttact caagtgctcc
     1681 aacctcgaag taggaatgaa cttcctgtgc agccggcgga gacaactacc actcctgata
     1741 taaaaaacac gactgtaact cccagtgtaa ccaaagatgt cgcactggct gccgttctgg
     1801 tccctgtgct ggtcatggcc ctcaccacac tcatcctcat tctagtgtgt gcttggcact
     1861 ggagaaacag gaagaagaaa actgaaggcg cctatgattt accccactgg gatcgggcag
     1921 gttggtggaa aggaatgaag cagcttctcc ctgccaagtc ggtggaccac gaggagacgc
     1981 cagtgcgcta cagcactagt gaagtcagtc acctgagtgc cagggaagtc accacagtgc
     2041 tgcaggccga ctctgcagaa tatgcacagc ccctcgtggg aggaattgtt ggcacactcc
     2101 atcagagatc cacctttaaa cctgaggaag ggaaggaagc aggctatgca gacctcgatc
     2161 cttacaactc tccaatgcag gaagtgtacc acgcctatgc tgaaccactg cccgtaacgg
     2221 ggcctgagta cgcaaccccg atcgtcatgg acatgtcagg gcaccccaca gcctcagttg
     2281 gtctgccctc cacatccacc ttcaaaactg cagggaccca gcctcacgct ttagtgggaa
     2341 cttacaacac tcttctctcc aggactgaca gctgttcctc aggccaggct cagtatgaca
     2401 ccccaaaagg tgggaagtca gctgctaccc cagaggaact ggtataccag gtgccccaga
     2461 gcacccagga gctatcagga gcaggaaggg atgagaagtt tgatgctttt aaagaaatcc
     2521 tttgacggtg gtgctgcttt ctgacaaggc atccatttaa agcacatggc ttttttcatt
     2581 cttagtggta gtgatgtaga atgtattacg tatctgtcac tgaagtggtt aggggaatgt
     2641 ggtgaccgag gtacaagaaa ctacttgcct tgccatcttg ctttaaaggg gttactgtgt
     2701 gacacagtta ctgctcgcct gctacatgtc aagcgtcctg tttgttacta ctgtaaaaca
     2761 ctatttttaa tacagaaaca gagctcccta taatgcactt cagagaaact gaaaatcact
     2821 gagtatttat taccaatgct gcaggtacat taatgaactt gaggtggttc tgtaagcctt
     2881 gctggcaata atgcatggta ctgttcttga aataccgaat ggcttgaaat tctccctttg
     2941 tgaaagtggg tactatcacg cgtcccttct cctttaatcc tgtagtgccg tctgcattct
     3001 tttttttttt ttttctcaca tccatcagtg ataagcattg ctctagctgt ggccacatac
     3061 acttctctgt atttccctag gaaggtgcag agttagaaca gcctgccttg cggtgagctg
     3121 gaggactgac tccttgtttg atgacacttt taacagtatt gacaccgcct catacagggc
     3181 ttggtctcag ctctgctcat ccctgccacc cttggtggcc ttgctccaac ctgtgttcat
     3241 aaaccctggc agctcctatc cagcagcagt cacgggaagc cagcccttcc catctttaag
     3301 tatacttctc cacagggaac tgggagagaa gcaacttacg atgtagaaaa gctataaagg
     3361 gagtcctctg cgtcactaaa gtaatctgta aattacttta gggacaaagt taagggggaa
     3421 ctaaaattta accttctttg ctttctttac acacaagccc aaaaatttgc aaaatttttt
     3481 ttaaatttca attaaatttt taaatttaat tttaaaccat tgtaagaaaa agaaagccaa
     3541 catgagccag gtctgatggc tcaagccagc aatcccaact gacccaagtg gacccccaag
     3601 gtcagtgctt tatagtctac agagtgagtt caggattaac ctaggccgct catgggataa
     3661 ggggtggagc tcaggctccg gggtttagtg ctccagtata acaagttcaa ggtcataggt
     3721 tcaatcctct ctactacaaa aagaaaaacg ggcaacaggc gttctccagg tctgtgtgat
     3781 ttctgccttc tgtctgggga gacacttaag tggtcctttg tgtcagacct ggttgacaga
     3841 gatgctgtgc gatcggaatt gtgtggaatc cttcccagca acttacttgt cagtgcttga
     3901 caaccgggct gcactcacgt caggtcgggt aaattaaatg ttacagcgga ttagcactga
     3961 agcagcacat cggatgaaaa cacaaacttg atccctcgcc acaaacctgt gtgtaggttt
     4021 agagtacact cggccttcag gtccccagcc gtttctcagc ttgtaccatc ctggttgtag
     4081 acctgccaca cttgtctaga tttggatagc cttgaacagt gttaactgtg cttccttcca
     4141 caagtacatc agcgttttaa aacgtcgctc tgcactggtg gcaggctctc tgccacctcc
     4201 tcagacagtt tgcatcgctt tcattataaa ccgcctttcg tcttgcagag tctcggacac
     4261 agtctctctc cttgttgaaa aaaatcagtg cacattttgt ttcaaagttc ttatgaagca
     4321 aaaaaaagca cagatgctgt actaaaacat gtaagaatgt gtagagcaca agatgcagtt
     4381 gtcctctttc aggtgggaac aaagcaaacc tttagctgtt tcattttcgt tcacgcttag
     4441 tgggcagagg gtagcctttt ctttgagtca cctaaccatt ggaagcttta agagaccctt
     4501 tgaagatgaa gttgttttcc caaagaagcc tactattcta actgtgtgtc ccagaactgt
     4561 ttcagaacag agtgaggcca tggttgttga tgcgttacac attgtgagac ttgactccta
     4621 tggccactgc tctccttgca ctctaccaat ggttttcata acatacctag ttattgttat
     4681 atggttttct cttgcagata aagagctgag cctgattcaa atgatttttt ttttctggtt
     4741 ttgtactttt ccttttaatt ttttgtatta tagattacat gtctggtaaa tctttggaag
     4801 acaatcaagt aaaatgtgaa agtaaccatt gctgaccaaa ccccttggtt attttgtttt
     4861 gctttaattc tttgcttgag atattcaata cttaagtcac tgtggcttag tgggtttctc
     4921 ttttcctgat agtacaattg attagagaca gcagtgtggc aagggcagcc ttaggagatt
     4981 tcagaaaagt tacactaact tggctgtcac ttgatccttg gaaccagcac agatattatg
     5041 aattctgctt tgcatttatt ttaaatgctg tttatagatc gtagtagttc ctgttgagag
     5101 taacaggact agtccagtta tttaattcat gtttcactat ttgcactttc cactgaacag
     5161 tgggttgatt tgctgttcac acacagaatt tatccagtag ttacagctat catgtgttct
     5221 gtgtatgctc ctcctccacc tgcccccagg gatgaagcag taaaagtgct tgtagccatt
     5281 tccatgcctt ggctccacgt tgtcacccag agagcagtaa agggagtagg aaagcaagat
     5341 agaaaaaaga cagacaagac cactagaacg aacccagaaa ttatgggggg gtgggaggtt
     5401 ccaaatcctc tacggagcag aactaacggg gctccgctgt atggaaagga gttgttaatt
     5461 ctaccttttg ggttgaactt gaaagtcctt gaactgcaaa aactctagga agttggatgg
     5521 ttgtagggta tctaggttaa ctgtggcacc aatctcaatt agaccatttt gtgtgtagtt
     5581 tctctcgttt cctgtgtttg gcactgggaa tggtgaaaat aaaacttact caagtacaga
     5641 agaagtggaa gtggctgagt gtcagtgata ttcccccaga atgtattttt cttacctcgg
     5701 catgtactgt agtcacttga tatttgtata tgttgctaga atttaggctg ttgaaaatag
     5761 tgagattcta atgtgttttt aataaattat atgtgtgtgt cttgctcaga tttttttgga
     5821 ttaaataaaa caaccttgac atcatagtcc agaaaatgcc aaatttctca aagttgacaa
     5881 cagtcacatt ttatgaccag tattatagtg ggtatagata gcatcattgg tccccctgcc
     5941 actgtccctg ccggcaactc cacaaccctc aagcctacct tatggaagtg aatctgggca
     6001 gcccagctct gtgtggctat agagtaaagg ggcacacccc atcagcatgg agagccagcc
     6061 agtgccagcg tccccatcag ttaggtgctg gacactgcca gcatgagcta ccctcactgt
     6121 cagacccctc ctatttacag tttgtcagta tttcatttat tctttttctg acatttcact
     6181 ctttttattt tacacacaaa ctctttaggg aggaggcaaa ttgattgcca ttatcaccag
     6241 cgtttccaat aataacactt cctgtttgct ctcctcccat tctaccccct ttggttgtgt
     6301 ctactaacag ttgaaaggtg gaacttgcct cgcaggccac catttgtttg tgtttcttca
     6361 gatttttctc tcatgtgcct gaaatgacca ctgaatgttt cactgaaggt gaaatttagt
     6421 catgtgaaat aggacatttt tttccctata caaataataa aaaaaaaagc tcaactctta
     6481 a
//
