LOCUS       NM_177267               5706 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus DDB1 and CUL4 associated factor 5 (Dcaf5), transcript
            variant 1, mRNA.
ACCESSION   NM_177267
VERSION     NM_177267.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Radko-Juettner,S., Yue,H., Myers,J.A., Carter,R.D., Robertson,A.N.,
            Mittal,P., Zhu,Z., Hansen,B.S., Donovan,K.A., Hunkeler,M.,
            Rosikiewicz,W., Wu,Z., McReynolds,M.G., Roy Burman,S.S.,
            Schmoker,A.M., Mageed,N., Brown,S.A., Mobley,R.J., Partridge,J.F.,
            Stewart,E.A., Pruett-Miller,S.M., Nabet,B., Peng,J., Gray,N.S.,
            Fischer,E.S. and Roberts,C.W.M.
  TITLE     Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing
            SWI/SNF
  JOURNAL   Nature 628 (8007), 442-449 (2024)
   PUBMED   38538798
  REMARK    Erratum:[Nature. 2024 May;629(8012):E12. doi:
            10.1038/s41586-024-07402-3. PMID: 38684813]
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Guo,P., Lim,R.C., Rajawasam,K., Trinh,T., Sun,H. and Zhang,H.
  TITLE     A methylation-phosphorylation switch controls EZH2 stability and
            hematopoiesis
  JOURNAL   Elife 13, e86168 (2024)
   PUBMED   38346162
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5706)
  AUTHORS   Zhang,C., Leng,F., Saxena,L., Hoang,N., Yu,J., Alejo,S., Lee,L.,
            Qi,D., Lu,F., Sun,H. and Zhang,H.
  TITLE     Proteolysis of methylated SOX2 protein is regulated by L3MBTL3 and
            CRL4DCAF5 ubiquitin ligase
  JOURNAL   J Biol Chem 294 (2), 476-489 (2019)
   PUBMED   30442713
  REMARK    GeneRIF: Knockdown of L3MBTL3 or DCAF5 is sufficient to restore the
            protein levels of SOX2 and rescue the defects of mouse embryonic
            stem cells caused by LSD1 or PHF20L1 deficiency.
REFERENCE   4  (bases 1 to 5706)
  AUTHORS   Leng,F., Yu,J., Zhang,C., Alejo,S., Hoang,N., Sun,H., Lu,F. and
            Zhang,H.
  TITLE     Methylated DNMT1 and E2F1 are targeted for proteolysis by L3MBTL3
            and CRL4DCAF5 ubiquitin ligase
  JOURNAL   Nat Commun 9 (1), 1641 (2018)
   PUBMED   29691401
  REMARK    GeneRIF: L3MBTL3 binds the K142-methylated DNMT1 and recruits a
            novel CRL4(DCAF5) ubiquitin ligase to degrade DNMT1.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK137579.1 and CT030161.10.
            
            On Jun 14, 2018 this sequence version replaced NM_177267.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK122561.1, BC042567.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164138 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4865              AK137579.1         1-4865
            4866-5706           CT030161.10        174573-175413       c
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 36.61 cM"
     gene            1..5706
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /note="DDB1 and CUL4 associated factor 5"
                     /db_xref="GeneID:320808"
                     /db_xref="MGI:MGI:2444785"
     exon            1..417
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     CDS             204..3044
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /note="isoform 1 is encoded by transcript variant 1; WD
                     repeat domain 22; WD repeat-containing protein 22"
                     /codon_start=1
                     /product="DDB1- and CUL4-associated factor 5 isoform 1"
                     /protein_id="NP_796241.3"
                     /db_xref="CCDS:CCDS26013.1"
                     /db_xref="GeneID:320808"
                     /db_xref="MGI:MGI:2444785"
                     /translation="MKRRAGLGGSMRSVVGFLSQRGLHGDPLLTQDFQRRRLRGCRNL
                     YKKDLLGHFGCVNAIEFSNNGGQWLVSGGDDRRVLLWHMEQAIHSRVKPIQLKGEHHS
                     NIFCLAFNSGNTKVFSGGNDEQVILHDVESSETLDVFAHEDAVYGLSVSPVNDNIFAS
                     SSDDGRVLIWDIRESPHGEPFCLANYPSAFHSVMFNPVEPRLLATANSKEGVGLWDIR
                     KPQSSLLRYGGNLSLQSAMSVRFNSNGTQLLALRRRLPPVLYDIHSRLPVFQFDNQGY
                     FNSCTMKSCCFAGDRDQYILSGSDDFNLYMWKIPADPEAGGIGRVVNGAFMVLKGHRS
                     IVNQVRFNPHTYMICSSGVEKIIKIWSPYKQPGCTGDLDGRIEDDSRCLYTHEEYISL
                     VLNSGSGLSHDYANQSVQEDPRMMAFFDSLVRREIEGWSSDSDSDLSESTILQLHAGV
                     SERSGYTDSESSASLPRSPPPTVDESADNAFHLGPLRVTTTNAVASTPATPTCEDASS
                     RQQRLSALRRYQDKRLLALSNESDSEENVCEAELDTDLFPRPRSPSPEDGSSSPSSST
                     SSEDEEELNQRRATTRQRNAMRRRQKTARDERPTGPAKSTNTYIGEDNYDYPQIKVDD
                     LSPSPDSSPERSASTLDVQPSRAPPAANMESVERKIYKAYKWLRCSYISYSNNKDGET
                     SLMAEEADEGRAGTSHKDNPTPSSSKEACLTATAQRDQDLPSEGCSSDACKEGTSAGN
                     PNSGAGHEHSSHPWAEVPEGTSQDTNNSGPLEHSFETKKLNGKALSKALSSRAEEPPA
                     SPGPKASGSTLNSASGNCPRTQSDDSEERSLDTVCANHNNGRLHPRPLHPHNNGQSSG
                     ELETVACSSPGHLDTDHDSPSLTGTFLHKDCCGSEMACETPSAGMREDPPDPSGLDSS
                     KVVHGQSGLKRHRIELEDTESENSSSEKKLKT"
     exon            418..561
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     exon            562..598
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     exon            599..738
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     exon            739..868
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1082
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1149
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1277
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..5706
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4834..4839
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /note="hexamer: ATTAAA"
     polyA_site      4872
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /note="major polyA site"
     regulatory      5686..5691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
                     /note="hexamer: AATAAA"
     polyA_site      5706
                     /gene="Dcaf5"
                     /gene_synonym="9430020B07Rik; BCRG2; BCRP2; Wdr22"
ORIGIN      
        1 gagggacgcg accgtgagga agccgcgaac gccgccgacc gaccgtctgc acacctcgct
       61 ccgtctgcca tggctggtta aagaagcctc cggctcgctg cgcgcgggga gggacgggac
      121 gggaggggag cggcggcccg gcgtgcggcc gagggagggg cggcggcgcg agctgcagcg
      181 gcggtggcgg cggcggttcc agcatgaaga ggagagctgg cctggggggc agcatgaggt
      241 cagtggtggg cttcttgtcc cagcggggct tgcatgggga ccccctgctc actcaggact
      301 ttcagaggag acgcctgcgg ggctgcagaa acctctacaa gaaggacctt ctcggccact
      361 tcggctgtgt caatgccatt gaattctcca acaatggagg ccagtggctg gtctcaggag
      421 gagacgaccg ccgagttctg ctatggcaca tggaacaagc tatccattcc agggtcaaac
      481 ccatacagct gaaaggggaa caccattcca acattttctg cctggctttc aacagtggga
      541 acaccaaagt attctctgga ggaaatgatg agcaagttat cctccatgat gttgaaagca
      601 gtgagaccct ggatgtgttt gctcatgaag atgcagtata tggcttgtca gtgagccccg
      661 tgaatgacaa catttttgcc agctcttcag atgatggccg ggttctcatt tgggatatcc
      721 gggagtctcc ccatggagag cccttctgcc tggcaaacta tccgtcagcc tttcacagcg
      781 tcatgtttaa ccccgtggag ccccggctat tggccacagc caattcaaag gaaggagtgg
      841 gactctggga cattagaaag cctcagagtt ctctcctgcg ctatggtggc aacctgtctc
      901 tccagagtgc catgagtgtg cgattcaaca gcaatgggac ccaactcctg gcactgcggc
      961 gccgcctgcc ccctgtcctc tatgacatcc actcccgcct gccagtgttc cagtttgaca
     1021 atcagggtta cttcaactca tgcactatga aaagctgctg ctttgccggg gatcgtgacc
     1081 agtacatcct ttcaggctcc gatgacttca acttgtacat gtggaagatc cctgcagatc
     1141 cagaggcagg tggcattggc agggtggtca atggagcctt catggtgctg aaagggcatc
     1201 ggtccatcgt gaaccaggtt cggtttaacc cccacaccta tatgatctgc tcttctggcg
     1261 tagaaaagat tatcaagatc tggagcccat acaagcagcc aggatgtaca ggtgacctgg
     1321 atggtcggat tgaagatgac tcccgctgcc tctacaccca tgaggagtac attagccttg
     1381 ttctgaacag tggaagtggc ctgtcccacg actacgctaa ccagtcagtc caggaggacc
     1441 ctcggatgat ggctttcttt gactccttgg tgcgtcgtga aattgagggc tggagctcag
     1501 actcggacag cgacctcagc gagagcacta tcctccagct gcacgcaggg gtcagtgagc
     1561 gctcaggcta cactgactcg gaatcttcag cctcactgcc ccgctcccca cctcccacag
     1621 tagacgagtc tgcggacaac gccttccacc tggggcccct gcgcgtcact accacaaatg
     1681 cagtggcgtc aaccccagca acgccaacat gtgaggatgc gtcctctcgg cagcagcgcc
     1741 tgtctgctct gaggcgttac caggacaaac gcctcttggc cctgtccaat gagtccgatt
     1801 ctgaagagaa tgtttgtgag gctgaactgg acacagatct cttccctcgg ccacggtcgc
     1861 ccagccctga ggatgggtcc agcagtccca gcagctccac cagctctgag gatgaagagg
     1921 aactgaacca acgccgagcc accacccggc agcggaacgc catgcggagg cgacaaaaga
     1981 cagcccggga tgagcggccc actggtccag ccaagtccac caacacctac attggagagg
     2041 acaattacga ctacccacag atcaaggtgg atgacctctc cccctcccca gactcctccc
     2101 ctgagcggag cgcttccact ctggatgttc agccaagccg ggcaccacca gccgccaaca
     2161 tggaatcagt tgagcgtaag atttacaaag cttacaaatg gctccgctgc tcctatatct
     2221 cttactcgaa taacaaagat ggggaaacct ccttaatggc agaggaggca gatgaaggaa
     2281 gagcaggaac cagccacaaa gacaacccaa ccccttcttc cagtaaggaa gcctgtctaa
     2341 cagccacagc ccagagggac caggacctgc cctctgaagg ctgcagcagt gatgcttgta
     2401 aagaagggac ttccgccgga aatcccaaca gcggtgcagg ccatgagcat agtagccatc
     2461 cttgggcaga ggtgccagag ggcacctctc aggacactaa caacagtggc ccactagaac
     2521 actcttttga aaccaagaag ctcaatggaa aggctctgag caaggccctg agcagtcggg
     2581 ctgaggagcc acccgcctct cctggcccca aggcatcggg ctctactctc aacagtgcat
     2641 ctggcaactg tcccaggacc cagtctgatg acagtgagga gaggagcctt gacactgtct
     2701 gtgccaacca caacaatgga cgcttgcacc ccaggcccct gcacccccac aacaatgggc
     2761 agagctctgg ggagctggag acagtggcct gctcttcccc aggacaccta gacactgacc
     2821 atgatagccc atccctgaca gggacatttc tacacaaaga ttgttgtggg tcggaaatgg
     2881 cctgtgagac cccaagtgct ggaatgagag aggacccccc tgacccttca ggcctggaca
     2941 gcagcaaggt tgttcatggc caaagcggcc tcaaaaggca ccgaattgaa ttggaagata
     3001 cagagtcaga gaattcctcc tcagagaaga aattaaaaac atgataaata caaagggaaa
     3061 acaaaagcta caaaaagtag ccttacagaa taatttttct tgtttagtga acacagagga
     3121 aaggaaaaag tattaaaggc acataaaacc agggcctgaa acgtgtctgc tgctgctcct
     3181 tctgttagcc gctctccagt gggtcagcat gtctagctgg cactgcctcg ctctctgctg
     3241 agggcactgc cagcggctct tctctagtga gatgtgagca tagcattcct gtgcaggttc
     3301 tgtgacgatg agcccgtctt gggacattcg gcttgttctg tgtgtgtgtg tccactagca
     3361 catgtgggct tgttgtctta ggagaccttt cttttaactg tccatcagca aagacctgtg
     3421 aatctgtagc ttttgccgtc acacctgtcc attgttcatt tgacatggtg tcctggtcct
     3481 aacagtgtct cttagatcag attgtgctgc ggtgagttgt aaatttatgc tagcagaaag
     3541 gagatacaaa tggaaagagg ccttccttca gctcattggc tgtgctgaag aggaaaacat
     3601 ggctcctttc ttaaatgttc catgttttct ccagctgaca tatcgctgag cttctaccgt
     3661 ggctctaagt ggagcattgc ccatgctaat aagaggttga tgtggaagct cagatcctga
     3721 gagtgtcact gtgtgcagaa ggggcagagc tacgatatgt gctgtgaggt gccgtacaat
     3781 gactagtgga caacacacta accatctagg tgctgggttc ctaagaggca cgcaaggcca
     3841 gcaggtgctt ccttggctcc aggtaatgga gtccaggcac actgaaagga aggaccccct
     3901 tgctctaata aaatggctgg tagaagaatg ctaatgagct atagtggcga gcagaatgtg
     3961 gtactgtgtt ctagtccagt gccaattcat cagtcagaaa tgggggctgg caccttctct
     4021 caggcgtgga gttgtcagag gctgtgggtt ccacctgaac tttgcagtgt ggtaacgtct
     4081 gttaggggac aggggatatg gagagacaag tctctggttt gtatcctggc tcccagaaga
     4141 cattctgttc tgttgaagta ggtaacaatg gagagaagaa aatatcaaac ttccagtgtg
     4201 tagtggtgat tgtagtcccc gagttgtcag tgaagggttg tgcaagacag gaagacctaa
     4261 ttggtgtaga tgttaaaagc aagaggaaga tagaacagtg gaactttgga tgcagaggtg
     4321 ctagacagct ggagcttggc tggaactggc atagggagga cttagcacag gcagctgtgg
     4381 cctccgtgag ccagcgttgc tttctgagga ggacctgtcc tgctggaatg gagccgagag
     4441 aagaccacct gtgaaggctg aagaacggac ggacagacgg acggatgctg ctcagtgctg
     4501 agagccaggg ttcttataaa ggaaggcagt tggtaagagc cagggggatg tgtggcatct
     4561 ttctgagctt cgcagaaggg agatgtagcg gctggagtct agaagcacgg tttgagaagg
     4621 agaggaagga aggcgcctga gtagggtggg ggtggatacg gtcagcagct ggactcttcc
     4681 tgggaagtga gacagtgact ctgtaaacaa cgttagaatt actttagatt tgtaatcaaa
     4741 atggttctat gatttatctt ttgactattc acagataatt gtaaattctg gttttataag
     4801 cccaagtcat tttacgtttg ccttttcaaa attattaaat tggaattttt taatccttta
     4861 tatacaaaaa aagttataac ccctcagtgt gtgtctctct tccccttagc ttacctggtt
     4921 cttctattct gttttacttt aaccagatag tttcacaata tcttcctgga ggaaaaagaa
     4981 agcaaaatta gctcttaata attagtttcc aattgccaag tatggtcttt cattgctaaa
     5041 cgctatctca tttgggtaat caaaaacatt caaaagtcag aggtcattga gtttgtaact
     5101 gtagatgcct ttgcggatgc tttccatcct gggagagact tccagcaccg taggtttggg
     5161 ttgtcaggag atgggcccca aattaactgc cagagcaggc agaaagggat gctgcgttca
     5221 gccattccac acttacttcc agagctcttg tgttgtgtgt attgcaagcc tggccagaac
     5281 tacttcaaag gcccctttac ctaaaccatt gtagaagcca ggcctccctt gttgaagaca
     5341 tatttaatcc ccaaattctt ccgtgatggg tgtttttccc agcaggagag ggcactgatc
     5401 agacagattt tgttcctgca tcctgtacat aatgttttga gtgtcaggtc cagggatgag
     5461 agaccgttgc tcttcccatt gtttctctcc tggttttgta aatgattctc aatcgacata
     5521 tgagttcaat gagctgttgg cttgaggcag gtgaagtttg caactgtgct gatttaatag
     5581 gagtctcgtg ttgtctgtca aggtgccttc ctctgctgaa gccttacctc ctcacagcct
     5641 gcctcttctc acccaacaat gctgactctt ctctgtgtat atgcaaataa acctattaat
     5701 cacttc
//
