LOCUS       NM_001373930            5697 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus DDB1 and CUL4 associated factor 1 (Dcaf1), transcript
            variant 1, mRNA.
ACCESSION   NM_001373930 XM_006511755
VERSION     NM_001373930.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Dar,A.A., Sawada,K., Dybas,J.M., Moser,E.K., Lewis,E.L., Park,E.,
            Fazelinia,H., Spruce,L.A., Ding,H., Seeholzer,S.H. and Oliver,P.M.
  TITLE     The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by
            aiding the repair of damaged DNA
  JOURNAL   PLoS Biol 19 (2), e3001041 (2021)
   PUBMED   33524014
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Guo,Z., Wang,G., Wu,B., Chou,W.C., Cheng,L., Zhou,C., Lou,J.,
            Wu,D., Su,L., Zheng,J., Ting,J.P. and Wan,Y.Y.
  TITLE     DCAF1 regulates Treg senescence via the ROS axis during
            immunological aging
  JOURNAL   J Clin Invest 130 (11), 5893-5908 (2020)
   PUBMED   32730228
  REMARK    GeneRIF: DCAF1 regulates Treg senescence via the ROS axis during
            immunological aging.
REFERENCE   3  (bases 1 to 5697)
  AUTHORS   Li,Y., Cui,C., Xie,F., Kielbasa,S., Mei,H., van Dinther,M., van
            Dam,H., Bauer,A., Zhang,L. and Ten Dijke,P.
  TITLE     VprBP mitigates TGF-beta and Activin signaling by promoting
            Smurf1-mediated type I receptor degradation
  JOURNAL   J Mol Cell Biol 12 (2), 138-151 (2020)
   PUBMED   31291647
  REMARK    GeneRIF: VprBP mitigates TGF-beta and Activin signaling by
            promoting Smurf1-mediated type I receptor degradation.
REFERENCE   4  (bases 1 to 5697)
  AUTHORS   Nowak,M., Suenkel,B., Porras,P., Migotti,R., Schmidt,F., Kny,M.,
            Zhu,X., Wanker,E.E., Dittmar,G., Fielitz,J. and Sommer,T.
  TITLE     DCAF8, a novel MuRF1 interaction partner, promotes muscle atrophy
  JOURNAL   J Cell Sci 132 (17) (2019)
   PUBMED   31391242
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5697)
  AUTHORS   Schabla,N.M., Perry,G.A., Palmer,V.L. and Swanson,P.C.
  TITLE     VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by
            Mediating RAG1 Degradation
  JOURNAL   J Immunol 201 (3), 930-939 (2018)
   PUBMED   29925675
  REMARK    GeneRIF: The basis for RAG1 turnover and provide evidence that the
            CRL4(VprBP(DCAF1)) complex functions to maintain physiological
            levels of V(D)J recombination.
REFERENCE   6  (bases 1 to 5697)
  AUTHORS   Kassmeier,M.D., Mondal,K., Palmer,V.L., Raval,P., Kumar,S.,
            Perry,G.A., Anderson,D.K., Ciborowski,P., Jackson,S., Xiong,Y. and
            Swanson,P.C.
  TITLE     VprBP binds full-length RAG1 and is required for B-cell development
            and V(D)J recombination fidelity
  JOURNAL   EMBO J 31 (4), 945-958 (2012)
   PUBMED   22157821
  REMARK    GeneRIF: multi-subunit cullin RING E3 ligase complex
            VprBP/DDB1/Cul4A/Roc1 associates with full-length RAG1 through
            VprBP
REFERENCE   7  (bases 1 to 5697)
  AUTHORS   McCall,C.M., Miliani de Marval,P.L., Chastain,P.D. 2nd,
            Jackson,S.C., He,Y.J., Kotake,Y., Cook,J.G. and Xiong,Y.
  TITLE     Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a
            WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is
            essential for DNA replication and embryonic development
  JOURNAL   Mol Cell Biol 28 (18), 5621-5633 (2008)
   PUBMED   18606781
  REMARK    GeneRIF: Data show that human immunodeficiency virus type 1
            Vpr-binding protein VprBP binds stoichiometrically with DDB1
            through its WD40 domain and through DDB1 to CUL4A, subunits of the
            COP9/signalsome.
REFERENCE   8  (bases 1 to 5697)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   9  (bases 1 to 5697)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 5697)
  AUTHORS   Zhang,S., Feng,Y., Narayan,O. and Zhao,L.J.
  TITLE     Cytoplasmic retention of HIV-1 regulatory protein Vpr by
            protein-protein interaction with a novel human cytoplasmic protein
            VprBP
  JOURNAL   Gene 263 (1-2), 131-140 (2001)
   PUBMED   11223251
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC147636.3.
            
            On Sep 5, 2019 this sequence version replaced XM_006511755.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.9648.1,
                                           SRR9219381.245128.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-168               AC147636.3         113293-113460       c
            169-286             AC147636.3         106206-106323       c
            287-363             AC147636.3         104450-104526       c
            364-437             AC147636.3         101162-101235       c
            438-551             AC147636.3         99755-99868         c
            552-689             AC147636.3         98686-98823         c
            690-1199            AC147636.3         96676-97185         c
            1200-1301           AC147636.3         96251-96352         c
            1302-1460           AC147636.3         91090-91248         c
            1461-1640           AC147636.3         88546-88725         c
            1641-1850           AC147636.3         87394-87603         c
            1851-2020           AC147636.3         83277-83446         c
            2021-2145           AC147636.3         81090-81214         c
            2146-3409           AC147636.3         76297-77560         c
            3410-3608           AC147636.3         75605-75803         c
            3609-3691           AC147636.3         74923-75005         c
            3692-3776           AC147636.3         74332-74416         c
            3777-4010           AC147636.3         72134-72367         c
            4011-4104           AC147636.3         71641-71734         c
            4105-4209           AC147636.3         70764-70868         c
            4210-4283           AC147636.3         70280-70353         c
            4284-4385           AC147636.3         69685-69786         c
            4386-4638           AC147636.3         61076-61328         c
            4639-5697           AC147636.3         54392-55450         c
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 57.98 cM"
     gene            1..5697
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /note="DDB1 and CUL4 associated factor 1"
                     /db_xref="GeneID:321006"
                     /db_xref="MGI:MGI:2445220"
     exon            1..168
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            169..286
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     CDS             177..4697
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein VPRBP; serine/threonine-protein kinase VPRBP; Vpr
                     (HIV-1) binding protein"
                     /codon_start=1
                     /product="DDB1- and CUL4-associated factor 1 isoform 1"
                     /protein_id="NP_001360859.1"
                     /db_xref="CCDS:CCDS90657.1"
                     /db_xref="GeneID:321006"
                     /db_xref="MGI:MGI:2445220"
                     /translation="MTTVVVHVDSKAELTTLLEQWEKDHGSGQDMVPILTRMSELIEK
                     ETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLN
                     TAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDI
                     AANYRDENSQLVAIVLRRLRELQLQEVALRQDSKRPSPRKLSSEPLLPLDEEAVDMDY
                     GDMAVDVVDGEQESSRDMEISFRLDSSHKTSSRVNSATKPEEGGLKKNKSAKHGDREN
                     FRKAKQKLGFSSSDPDRVFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLT
                     PLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVA
                     HGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMEC
                     SHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDE
                     IFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQ
                     IVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALD
                     VLAILTVVPKIQLQLAESVDVLDEAGSAVSTVGISIILGVAEGEFFIHDAEIQKSALQ
                     IIINCVCGPDNRISSIGKFISGTPRRKLSQTPKSSEHTLAKMWNVVQSNNGIKVLLSL
                     LSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKR
                     SDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNH
                     LISKGLGETATVLTREADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIASRLGS
                     HATVGASAPSAPPAHPPPRPPQGSLPLPGPSYAGNSPLIGRISFIRERPSPCNGRKIR
                     VLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSL
                     FTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREA
                     NEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDG
                     SLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDI
                     QTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNIS
                     GVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDL
                     LEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALN
                     MDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAEL
                     EEDDNNENAGEDGDNDFSPSDEELANLLEEGEEGEDEDSDADEEVELILGDTDSSDNS
                     DLEDDIILSLNE"
     exon            287..363
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            364..437
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            438..551
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            552..689
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            690..1199
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1301
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            1302..1460
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            1461..1640
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1850
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..2020
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2145
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            2146..3409
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            3410..3608
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            3609..3691
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            3692..3776
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            3777..4010
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            4011..4104
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            4105..4209
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            4210..4283
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            4284..4385
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            4386..4638
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     exon            4639..5697
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5666..5671
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /note="hexamer: AATAAA"
     polyA_site      5690
                     /gene="Dcaf1"
                     /gene_synonym="B930007L02Rik; mKIAA0800; Vprbp"
                     /note="major polyA site"
ORIGIN      
        1 gagtgacgca taatatgtgg gccgctcagg agccgtggtc gcgctgtgtg tgtgtatatg
       61 tgtgtgtgtg agtgagtgag ggagagtgag tgcgggacca gaggcagtgt gtgagaaccg
      121 tggccgctgg aggggtcggg gggagaagcg gcggagccgc tcgcgaaggc caagtcatga
      181 ctacagtagt ggtacatgtg gactccaaag ctgagctcac taccctgctg gagcagtggg
      241 aaaaggacca tggcagtggc caggatatgg taccaatcct taccagaatg tctgaattaa
      301 ttgaaaaaga aactgaagaa tatcgtaaag gagatccaga tccatttgat gatcgacatc
      361 ctggtcgagc tgatccagag tgtatgctgg gccatttgct gagaatactc ttcaagaatg
      421 atgatttcat gaatgcgctg gtaaatgcat atgtgatgac aagtagagag ccccctttaa
      481 atactgcagc ttgcagactg ctgctggaca tcatgccggg cctagaaacc gcagttgtct
      541 ttcaagaaaa ggaaggaatt gttgaaaatc ttttcaaatg ggcccgagag gctgatcagc
      601 cacttaggac atattctact ggactgctgg gaggtgctat ggaaaatcaa gatattgctg
      661 ccaactacag agatgaaaat tcacagttgg tggcaatagt gcttagaaga ctgagggagc
      721 tccagcttca agaagtggct ttgcggcagg acagtaagcg tcctagtcca cggaagctct
      781 cttcggaacc ccttttgcct ctggatgagg aagctgtgga tatggactat ggtgacatgg
      841 cagtggatgt tgtggatgga gaacaagaat cttctagaga tatggagatc tcctttcgtc
      901 ttgattcaag tcacaagacc agtagtagag tgaactcagc aactaaacct gaggaaggag
      961 gattaaagaa aaacaagtca gcaaagcatg gtgacagaga gaactttagg aaggccaagc
     1021 agaagttggg cttctcatct tctgacccag atcgtgtgtt tgttgagctg tctaatagca
     1081 gctggtcaga aatgtccccc tgggtgatcg gcaccaatta cactctctat cctatgactc
     1141 ctgctattga gcagagactt attcttcagt atttgacccc tttaggagag tatcaggagt
     1201 tacttcccat attcatgcaa cttggatcaa gagaattgat gatgttttat attgatctga
     1261 aacagacgaa tgatgtcctg cttacttttg aggcactcaa gcacctagca tctctcctgc
     1321 ttcataacaa atttgccaca gaatttgttg cacatggtgg agtacagaaa ttactggaaa
     1381 ttcctcgtcc ttctatggct gcaactggtg tgtccatgtg cttgtattac ctgtcctaca
     1441 atcaggatgc tatggaaaga gtttgcatgc atcctcacaa tgttctatct gatgtggtga
     1501 actataccct gtggttaatg gaatgttctc atgcttcagg atgttgccat gctactatgt
     1561 ttttctcaat ttgtttctca ttccgggctg ttttggagct ctttgaccgt tatgatggtc
     1621 ttcgtcgtct ggtgaacttg atcagtactt tggagatttt aaatttggaa gaccagggtg
     1681 cacttttgag tgatgatgaa atatttgcta gtcgccaaac aggaaaacat acctgcatgg
     1741 ctctgcgcaa gtattttgag gctcatctgg ccattaaatt ggaacaagtc aagcagtcac
     1801 ttcagaggac tgagggtggt attcttgttc acccccagcc tccatacaag gcatgctcat
     1861 atactcatga gcagattgta gaaatgatgg aatttttgat agagtatggc ccagctcagc
     1921 tatattggga acctgctgaa gtcttcctca agctttcctg tgtgcagctt ttactacagc
     1981 ttatttccat cgcctgcaat tggaagacct attatgcaag gaatgatact gtgcgttttg
     2041 ctttggatgt ccttgctatt ctcactgtgg tgccaaaaat ccagctccag ttggcggagt
     2101 cagtggatgt actggacgag gccggctctg ctgtctccac tgtaggtatc agcattattt
     2161 tgggcgtggc tgagggtgag ttctttatcc atgatgctga aattcagaag tcagcacttc
     2221 aaattatcat caattgtgta tgtggtccag ataaccgaat atccagtatt ggcaaattta
     2281 tttctggaac tcctcggaga aagctgtctc agactcccaa gagtagtgag catactctgg
     2341 ccaagatgtg gaatgtggtc cagtccaaca atggcatcaa ggtgcttctg tccctgttgt
     2401 ccatcaagat gcccattaca gacgcagacc agatccgggc cctggcctgc aaagcccttg
     2461 taggcctgtc tcgaagtagc actgttcgac agatcatcag taaacttccc ctttttagta
     2521 gctgccagat tcaacagctg atgaaagagc ctgtgctgca ggataagcgt agtgaccatg
     2581 tcaagttctg caaatacgct gctgagctca ttgagcgtgt atcaggaaag cctcttctca
     2641 ttggcactga tgtgtctctc gcacgactgc agaaagcaga tgttgttgct cagtcgagga
     2701 tctccttccc tgagaaagag ctgctcctgt tgatacggaa ccatctcatt tctaaagggc
     2761 ttggagagac agcaactgtg ctgacgagag aggctgacct gcccatgact gctgcctcac
     2821 attcttctgc cttcacccca gtgactgctg ctgcttctcc tgtctctctt ccccggaccc
     2881 ctcggattgc caacggcatt gcaagtcgac tgggcagcca tgctactgtg ggtgcttctg
     2941 ccccttctgc tccccctgcc catcctccac cacggccccc ccagggttca ttgcctttgc
     3001 ctggcccatc atatgcaggc aattcccctt tgattggtag gatcagtttt atcagagaga
     3061 ggccatcacc gtgcaatggc aggaaaattc gagtgttgcg gcagaagtcg gaccatggtg
     3121 catacagcca gagccctgcc ataaaaaaac aattggatag acatcttcct tccccaccta
     3181 cgctggacag tatcatcaca gagtatctta gagagcaaca tgctcgatgc aagaaccccg
     3241 ttgccacctg tcctcctttt tctctcttta ctcctcatca gtgtcctgaa ccaaaacaga
     3301 ggcggcaagc gccaataaac tttacctcaa ggctaaaccg cagggcatca tttccaaaat
     3361 atggaggggt agatggcgga tgctttgata ggcaccttat ctttagcaga tttcgtccga
     3421 tttcagtatt ccgggaagct aatgaagatg aaagtggctt cacctgttgt gcattctcag
     3481 cccgggagcg gttcctgatg cttggcactt gtacagggca gctgaagctc tacaatgtgt
     3541 ttagtggaca ggaagaggcc agctataact gtcacaactc agccatcaca caccttgaac
     3601 cgtcaaggga tgggtcctta cttctgacat ctgccacttg gagccagcct ttgtctgcac
     3661 tttggggaat gaaatcagta tttgatatga agcattcctt cacagaagat cactatgttg
     3721 agttcagtaa gcactctcag gatcgggtca taggcacaaa aggagacatt gcccatattt
     3781 atgatattca gactggcaac aagctgttga ctctgtttaa cccagatctt gccaacaact
     3841 acaagaggaa ctgtgccacc tttaatccta cagatgatct tgtcttaaat gatggcgtcc
     3901 tctgggatgt ccgctctgca caggccatcc acaagtttga caagttcaac atgaatatca
     3961 gtggtgtttt ccatccaaat gggctggaag tcatcatcaa tacagagatt tgggaccttc
     4021 ggactttcca tcttttacac actgttcctg ctctggatca atgccgtgtt gtttttaacc
     4081 acactgggac agtgatgtat ggagcaatgt tgcaggcaga tgatgaagat gacttactgg
     4141 aagagaggat gaaaagccct tttgggtcat cctttcgaac attcaacgca actgattata
     4201 aacctatagc aactattgat gtgaaacgaa acatctttga cctgtgcaca gacaccaaag
     4261 actgttatct tgctgtcatt gagaatcaag gcagcatgga tgcactgaat atggacacag
     4321 tgtgcaggct ctatgaagtg ggcaggcagc gtctggcaga ggatgaggat gaagaggagg
     4381 accaggaaga ggaagaacag gaggaagaag atgatgatga agatgatgat gatactgatg
     4441 atttagatga actagacact gaccagttgc tggaggcaga actggaggag gatgacaaca
     4501 atgagaatgc aggagaggat ggagacaatg acttctctcc ctctgatgag gagctagcaa
     4561 accttctaga ggagggagag gagggggaag atgaagactc tgatgcagat gaggaggtgg
     4621 agctgatcct gggggacaca gacagctcgg acaactctga tttggaagat gacatcatct
     4681 tatctctgaa tgagtgatgt gccattccta cttggaagag aatcttggca ggcgagagaa
     4741 actgagtgaa ttcagaacac ctgccctttg ctgcctccca gggctgtcag ttaaggaact
     4801 gtatgccctg cattcagagg actatgattt aaagtctccc tggaatctga gggcccttct
     4861 aagaacaaca acaacagtga ccacacgacc acacacacac acacacacac acacacacac
     4921 acacacacac acacacacac acacacacac acacacacac acacacacac agagctacag
     4981 ggactaggcc ctagtttgtg tccccctcct cctaggaagg gcatagcttt cctcaaggga
     5041 aaaacaaaca tgtctctggg tatttcacaa tatattttct ttgtataatg ttctttactt
     5101 aaaagaacaa gaaatagttt tttataaaac ttaaaaagaa aaaaaaaagt accaggcatt
     5161 tctaacgagg gtctgaactt atccaccagt ctcctatccc cgcacttcat tttctttgga
     5221 agaaaatgat gtctaaagaa catatagagg catttttaca tacgtattta aatgaagagg
     5281 aaaatcgtgg tttcataaat tgataaggat taagaatatt ttattataaa tataatatat
     5341 gatttttaac ctgttttgtt gcctcatatg ctgtcaagtt aatttgtttt cctttgtgcc
     5401 agagcgggaa tgggcggcat ggcctgagca ctgggaatgc ctaactccct gcctcggtgg
     5461 ccaggcagca gtcagtcgtg tggaccctgg ttttgtggag ttgagagaac tttaggggta
     5521 taaattcact ccctctcttt cctctttgtg attcagtttt tcaagccttt ttttttttcc
     5581 ctttcccttt ctccccaatg tggaaattac aaatcaaagg tctttttctt taatgtaaag
     5641 tgtatttatt taaaaaaaat tacaaaataa actacaagtc tgtctttgtt tatggcc
//
