LOCUS       NM_172644               3621 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_172644
VERSION     NM_172644.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Croon,M., Szczepanowska,K., Popovic,M., Lienkamp,C., Senft,K.,
            Brandscheid,C.P., Bock,T., Gnatzy-Feik,L., Ashurov,A., Acton,R.J.,
            Kaul,H., Pujol,C., Rosenkranz,S., Kruger,M. and Trifunovic,A.
  TITLE     FGF21 modulates mitochondrial stress response in cardiomyocytes
            only under mild mitochondrial dysfunction
  JOURNAL   Sci Adv 8 (14), eabn7105 (2022)
   PUBMED   35385313
REFERENCE   3  (bases 1 to 3621)
  AUTHORS   Kaspar,S., Oertlin,C., Szczepanowska,K., Kukat,A., Senft,K.,
            Lucas,C., Brodesser,S., Hatzoglou,M., Larsson,O., Topisirovic,I.
            and Trifunovic,A.
  TITLE     Adaptation to mitochondrial stress requires CHOP-directed tuning of
            ISR
  JOURNAL   Sci Adv 7 (22) (2021)
   PUBMED   34039602
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3621)
  AUTHORS   Rumyantseva,A., Motori,E. and Trifunovic,A.
  TITLE     DARS2 is indispensable for Purkinje cell survival and protects
            against cerebellar ataxia
  JOURNAL   Hum Mol Genet 29 (17), 2845-2854 (2020)
   PUBMED   32766765
  REMARK    GeneRIF: DARS2 is indispensable for Purkinje cell survival and
            protects against cerebellar ataxia.
REFERENCE   5  (bases 1 to 3621)
  AUTHORS   Nemeth,C.L., Tomlinson,S.N., Rosen,M., O'Brien,B.M., Larraza,O.,
            Jain,M., Murray,C.F., Marx,J.S., Delannoy,M., Fine,A.S., Wu,D.,
            Trifunovic,A. and Fatemi,A.
  TITLE     Neuronal ablation of mt-AspRS in mice induces immune pathway
            activation prior to severe and progressive cortical and behavioral
            disruption
  JOURNAL   Exp Neurol 326, 113164 (2020)
   PUBMED   31887305
  REMARK    GeneRIF: Neuronal ablation of mt-AspRS in mice induces immune
            pathway activation prior to severe and progressive cortical and
            behavioral disruption.
REFERENCE   6  (bases 1 to 3621)
  AUTHORS   Frohlich,D., Suchowerska,A.K., Spencer,Z.H., von Jonquieres,G.,
            Klugmann,C.B., Bongers,A., Delerue,F., Stefen,H., Ittner,L.M.,
            Fath,T., Housley,G.D. and Klugmann,M.
  TITLE     In vivocharacterization of the aspartyl-tRNA synthetase DARS:
            Homing in on the leukodystrophy HBSL
  JOURNAL   Neurobiol Dis 97 (Pt A), 24-35 (2017)
   PUBMED   27816769
REFERENCE   7  (bases 1 to 3621)
  AUTHORS   Seiferling,D., Szczepanowska,K., Becker,C., Senft,K., Hermans,S.,
            Maiti,P., Konig,T., Kukat,A. and Trifunovic,A.
  TITLE     Loss of CLPP alleviates mitochondrial cardiomyopathy without
            affecting the mammalian UPRmt
  JOURNAL   EMBO Rep 17 (7), 953-964 (2016)
   PUBMED   27154400
REFERENCE   8  (bases 1 to 3621)
  AUTHORS   Dogan,S.A., Pujol,C., Maiti,P., Kukat,A., Wang,S., Hermans,S.,
            Senft,K., Wibom,R., Rugarli,E.I. and Trifunovic,A.
  TITLE     Tissue-specific loss of DARS2 activates stress responses
            independently of respiratory chain deficiency in the heart
  JOURNAL   Cell Metab 19 (3), 458-469 (2014)
   PUBMED   24606902
  REMARK    GeneRIF: DARS2 depletion in heart and skeletal muscle leads to the
            severe deregulation of mitochondrial protein synthesis followed by
            a strong respiratory chain deficit in both tissues.
REFERENCE   9  (bases 1 to 3621)
  AUTHORS   Scheper,G.C., van der Klok,T., van Andel,R.J., van Berkel,C.G.,
            Sissler,M., Smet,J., Muravina,T.I., Serkov,S.V., Uziel,G.,
            Bugiani,M., Schiffmann,R., Krageloh-Mann,I., Smeitink,J.A.,
            Florentz,C., Van Coster,R., Pronk,J.C. and van der Knaap,M.S.
  TITLE     Mitochondrial aspartyl-tRNA synthetase deficiency causes
            leukoencephalopathy with brain stem and spinal cord involvement and
            lactate elevation
  JOURNAL   Nat Genet 39 (4), 534-539 (2007)
   PUBMED   17384640
REFERENCE   10 (bases 1 to 3621)
  AUTHORS   Carninci,P., Waki,K., Shiraki,T., Konno,H., Shibata,K., Itoh,M.,
            Aizawa,K., Arakawa,T., Ishii,Y., Sasaki,D., Bono,H., Kondo,S.,
            Sugahara,Y., Saito,R., Osato,N., Fukuda,S., Sato,K., Watahiki,A.,
            Hirozane-Kishikawa,T., Nakamura,M., Shibata,Y., Yasunishi,A.,
            Kikuchi,N., Yoshiki,A., Kusakabe,M., Gustincich,S., Beisel,K.,
            Pavan,W., Aidinis,V., Nakagawara,A., Held,W.A., Iwata,H., Kono,T.,
            Nakauchi,H., Lyons,P., Wells,C., Hume,D.A., Fagiolini,M.,
            Hensch,T.K., Brinkmeier,M., Camper,S., Hirota,J., Mombaerts,P.,
            Muramatsu,M., Okazaki,Y., Kawai,J. and Hayashizaki,Y.
  TITLE     Targeting a complex transcriptome: the construction of the mouse
            full-length cDNA encyclopedia
  JOURNAL   Genome Res 13 (6B), 1273-1289 (2003)
   PUBMED   12819125
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY781897.1, AK138809.1 and AK030952.1.
            
            On Sep 1, 2015 this sequence version replaced NM_172644.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030952.1, AK138809.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164140, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                BY781897.1         5-32
            29-3609             AK138809.1         2-3582
            3610-3621           AK030952.1         3572-3583
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 69.75 cM"
     gene            1..3621
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /note="aspartyl-tRNA synthetase 2 (mitochondrial)"
                     /db_xref="GeneID:226539"
                     /db_xref="MGI:MGI:2442510"
     exon            1..668
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            669..768
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            769..835
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            836..937
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1033
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1157
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1158..1204
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..1311
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1381
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1561
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1669
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1732
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1885
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2104
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2215
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2291
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..3621
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2700..2705
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /note="hexamer: AACAAA"
     polyA_site      2724
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
                     /note="major polyA site"
     polyA_site      3610
                     /gene="Dars2"
                     /gene_synonym="5830468K18Rik; aspRS"
ORIGIN      
        1 gctcgccggg cgtcagaggg actggcttgt ggatgtggtt tgtgtctggg tttgaacctc
       61 agagctgttt ggatttgttc gtcttcacct ttcaggaaaa tccaggatgg gccgccaggt
      121 ttgggaaagt tgaaacacga ggctgcacct ccttatattt ctgcttatgc cctggtcacc
      181 ttcgagatac gtgggaataa agggaggacg cgcgcctggt ggccggcctt ccggctgggg
      241 cgctctggca ctggcgctgc tgttgaggca tcttcggggc tcagccttgt ttaattaaag
      301 tgggaactga tgcactgaag gaagcaaagg ctttccccct acccactcac ctaaggtctc
      361 tggagacctt gcaggtcaat ctactttgta gatactagaa cgttcagcgg agcagggtct
      421 ggatgcattt gcgtgggaac tagaatttta agggttccca ggtattccga aaaccgagtt
      481 ttgagcacca ccacacctgc agcgtgggat tcgtgattgt ctctccggat cccgggactt
      541 caacatgtat cttggttttt ggttaagtcg tctatgcagg ggtttatcca gacccatcgg
      601 gaagaccatg cggccaatct ggggttctct ttccagaaat ctggcgttga gttcacaaag
      661 aattccagaa ttcagtagct ttgttgctcg gaccaacaca tgtggagagt tgcgttcgtc
      721 ccacttaggc caagaagtca ctctgtgtgg atggattcag taccgcaggc agaacacctt
      781 cttagtccta agagattgcc acggccttgt tcagattctc attccccagg atgagtcagc
      841 cgcatctgtg aggaggattt tgtgtgaagc ccctgtggag tctgtggtga gagtatctgg
      901 gacagtaatc tcccgtcctc caggacaaga gaatccgaaa atgccgacgg gtgagattga
      961 aatcaaagtt aaaacagctg agctcctgaa tgcctgcaaa aagctgccct ttgagattaa
     1021 agactttgtg aagaaaacag aggcgctccg tttgcagtat cgctacttag atctgcgcag
     1081 cttccagatg cagtacaact tgcggctgag gtcgcagatg gtcatgaaaa tgcgggaata
     1141 tctctgtaat ctgcacgggt ttgtggatat agaaacccca accttgttta agaggacccc
     1201 tggaggtgcc aaggagtttt tagtaccatc cagggaacct ggaaagtttt attccctccc
     1261 tcaaagtcct cagcaattta agcagcttct gatggttgga ggtttagaca ggtattttca
     1321 ggtcgctcgg tgttaccgag atgaaggctc aagaccggat agacagccag agttcactca
     1381 gattgacata gagatgtcct ttgtagagca gactggcatt cagcgtttag ttgagggttt
     1441 gctgcagtat tcctggcctg gtgataaaga ccctctagtg actcctttcc cttccatgac
     1501 ttttgctgag gcactggcca cctatggaac tgataagcca gatactcgct ttgggatgaa
     1561 gattgtggat gtcagtgatg tgttcagaaa cacagagctc agattccttc aggatgcact
     1621 ggctaagccc caaggaactg tcaaagcaat atgtgttcat gacggagcga aatacttaag
     1681 gaaggaagac attgagttca ttaggaagtt tgcagttcac catttcagtc aggaagtcct
     1741 acctatattc ctgaatgcca agaagaactg gagttcgcca tttgctaagt tcataatgga
     1801 ggaggaaaga ctggaattag ccagatcgat ggagatacaa gaagaagaca tagtgttact
     1861 gactgctggg gaacatgaga aagcatgctc tctgttggga aagttacggc tagagtgtgc
     1921 agaccttttg gagatgagag gagcggtgct ccgagaccca gcagtgttct cttttctttg
     1981 ggtggttgac tttccactct tccttgccaa ggaagagagt cccacagagc tggagtcagc
     2041 ccatcaccca tttactgctc ctaactccag tgacattcac ctcctctata ctgagccgga
     2101 aaaggttcgt ggccaacact atgacttggt tttaaacggc aatgagatag gaggtggttc
     2161 tgtccgaatc catgatgcac agctacagag gtacatcctg gaaacactgc taaaggaaga
     2221 tgtgaaattg ctttcccatc tgcttcaggc tttagactat ggggcacccc cacacggagg
     2281 aattgcctta gggttagaca gactggtatg tcttgtcacg ggagcaccca gcatcagaga
     2341 tgtcatagcc ttccctaaat cctaccgagg acaggacctt atgagcaatg ctccagactc
     2401 cgtgtctcca gaagagctga agccttacca tatccacgtc ttgtggccag cagactcaga
     2461 agaggagagt gcatccgcca caccttccaa acacttgagc tcttaacttt tgtcttctct
     2521 gcttcaccaa acttaaatta agatccagac ctgccacaga ctttagaacc aaatgtctgt
     2581 gtagaagcaa tcattaccga gtaagaaaga gaagctacac aattgtgact tggtcatgtg
     2641 taccattagg actgtctgtg gaagtttttt aattggaaaa ttgtcaaatg aaacaaacaa
     2701 acaaaaaaat ctctttattg agtatttaga gtcttcctgt tttccaagca tgtttctata
     2761 cccagataag ttatttagaa taaggataat acaacatggt tgcctctgta cactggccag
     2821 ttaatgcagc aaggtttcag ttcatctagc ctcattagaa ccataaatta aactttgtgg
     2881 taatctttca gccatcaata taatgttcta agtagtatag taggatcaaa aaaaaaaatt
     2941 acaagtaaca gaattctatt gtgttgtacc ttaagcaagc tacaaatgtc tcttagctgc
     3001 cccagctgct tatataatgt gaatgacact tgtcctggct gtctcacaga tctcttgttg
     3061 ccagaatcat gaggttacat ttgtggcaga tatgtgattt gaaatactgt aaaacaggac
     3121 tgaaatgcag ctgtcctaac cactgttcta ttgctgtgag gagacaccac aaccaaagca
     3181 actctttata aagcatttag ttggtgctgg tttatagttt tagctgtaag catggtggca
     3241 aacacagaca tgatatagaa gtagctgaga gttctacatc ctgacctgct gttgctggaa
     3301 gactgggcct ggcacagaat tatttgcttt cttatggctt ttcaagacag ttttcctggc
     3361 atgggctctt ttttatggtt tttttttgtt tgcctgtttt cgagacaggg tttttctgtg
     3421 tagccctgac tgtcctggaa ctcactctgt agaccagact ggccttgaac tcaaatccgc
     3481 ctgcctctgc ctcctaagtg ctgggattag catgtgccac cactgcctgt ggttcttgtt
     3541 tatggttttt caagacagga ttactctctg tagccctgaa acttgcttac ttaaaggctt
     3601 tcaaaaatca gtttctctgt g
//
