LOCUS       NM_177445               2249 bp    mRNA    linear   ROD 23-APR-2024
DEFINITION  Mus musculus aspartyl-tRNA synthetase 1 (Dars1), transcript variant
            1, mRNA.
ACCESSION   NM_177445
VERSION     NM_177445.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Guo,Y., Xiang,P., Sun,X., Liu,W., Zhou,J., Yin,B., Hou,L.,
            Qiang,B., Li,H., Shu,P. and Peng,X.
  TITLE     Docking protein 6 (DOK6) selectively docks the neurotrophic
            signaling transduction to restrain peripheral neuropathy
  JOURNAL   Signal Transduct Target Ther 9 (1), 32 (2024)
   PUBMED   38351062
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Frohlich,D., Suchowerska,A.K., Spencer,Z.H., von Jonquieres,G.,
            Klugmann,C.B., Bongers,A., Delerue,F., Stefen,H., Ittner,L.M.,
            Fath,T., Housley,G.D. and Klugmann,M.
  TITLE     In vivocharacterization of the aspartyl-tRNA synthetase DARS:
            Homing in on the leukodystrophy HBSL
  JOURNAL   Neurobiol Dis 97 (Pt A), 24-35 (2017)
   PUBMED   27816769
  REMARK    GeneRIF: Study provides an in-depth characterization of the DARS
            tissue distribution in mice revealing surprising heterogeneity
            across brain regions and neural cell types, addresses the paucity
            of knowledge around Dars gene expression, cell and systems
            physiology and associated pathophysiology related to the
            leukodystrophy Hypomyelination with Brain stem and Spinal cord
            involvement and Leg spasticity.
REFERENCE   3  (bases 1 to 2249)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   4  (bases 1 to 2249)
  AUTHORS   Kim,J.Y., Kang,Y.S., Lee,J.W., Kim,H.J., Ahn,Y.H., Park,H., Ko,Y.G.
            and Kim,S.
  TITLE     p38 is essential for the assembly and stability of macromolecular
            tRNA synthetase complex: implications for its physiological
            significance
  JOURNAL   Proc Natl Acad Sci U S A 99 (12), 7912-7916 (2002)
   PUBMED   12060739
REFERENCE   5  (bases 1 to 2249)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY276340.1, AK151027.1 and CO043663.1.
            
            On Nov 1, 2008 this sequence version replaced NM_177445.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008638.1, AK151027.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                BY276340.1         2-58
            58-2231             AK151027.1         2-2175
            2232-2249           CO043663.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 55.8 cM"
     gene            1..2249
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /note="aspartyl-tRNA synthetase 1"
                     /db_xref="GeneID:226414"
                     /db_xref="MGI:MGI:2442544"
     exon            1..265
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..1705
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /EC_number="6.1.1.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     aspartyl-tRNA synthetase, cytoplasmic; aspRS;
                     aspartate--tRNA ligase, cytoplasmic"
                     /codon_start=1
                     /product="aspartate--tRNA ligase, cytoplasmic isoform 1"
                     /protein_id="NP_803228.2"
                     /db_xref="CCDS:CCDS15253.1"
                     /db_xref="GeneID:226414"
                     /db_xref="MGI:MGI:2442544"
                     /translation="MPSTNASRKGQEKPREIVDAAEDYAKERYGISSMIQSQEKPDRV
                     LVRVKDLTVQKADDVVWVRARVHTSRAKGKQCFLVLRQQQFNVQALVAVGDHASKQMV
                     KFAANINKESIIDVEGVVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAI
                     RPEVEGEEDGRATVNQDTRLDNRVIDLRTSTSQAIFRLQSGICHLFRETLINKGFVEI
                     QTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAED
                     SNTHRHLTEFVGLDIEMAFNYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVSKQFPC
                     EPFKFLEPTLRLEYCEALAMLREAGVEMDDEEDLSTPNEKLLGRLVKEKYDTDFYVLD
                     KYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLE
                     KIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP"
     exon            266..323
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            324..416
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            417..519
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            520..622
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            623..703
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            704..763
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            764..875
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1010
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1158
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            1159..1305
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1348
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1429
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            1430..1541
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1613
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     exon            1614..2232
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2214..2219
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /note="hexamer: AATAAA"
     polyA_site      2232
                     /gene="Dars1"
                     /gene_synonym="5730439G15Rik; Dars"
                     /note="major polyA site"
ORIGIN      
        1 agagggagga gctggcggcg gcgcgcgaga gctcgctgtg gctctcgcga tatttccgaa
       61 gccaaggttc cacgcggaga ctgggggctt agaacccggg ctgggagggc gagactgtgc
      121 gggagggagc cgcttcttag cctgtcctac ggaagcctgc gagggagggt ggtatccact
      181 ccccaaatcc agtgtcctga tgcccagcac caacgccagc cgtaagggtc aggagaagcc
      241 gcgggagatc gtggacgcgg cggaagatta tgctaaagag agatatggga tatcttctat
      301 gatacaatca caagaaaagc cagatagagt tttggttcga gttaaggacc tgacagttca
      361 aaaagctgat gatgttgttt gggtccgtgc aagagttcat acaagcagag caaaagggaa
      421 gcagtgcttc ttagtcctga ggcagcagca gtttaatgtc caggctcttg tggcggtggg
      481 agaccatgca agcaagcaga tggttaaatt tgctgccaac atcaacaaag aaagtattat
      541 tgacgtagaa ggtgttgtga ggaaagtcaa tcagaaaatt ggaagctgta cccagcagga
      601 tgttgagttg catgtccaga agatttatgt gatcagcttg gctgaacctc gcctgccctt
      661 gcagctggat gatgccattc ggcctgaagt ggaaggagaa gaggacggaa gagctacggt
      721 taaccaggat acaagattag acaacagagt cattgacctt aggacatcaa ctagtcaggc
      781 catcttccgt ctccagtccg gcatctgcca tctcttccga gaaactttaa ttaacaaagg
      841 ttttgtggaa atccaaactc ctaaaataat ttcagctgcc agtgaaggag gagccaatgt
      901 ttttactgtg tcctatttta aaaataatgc ctaccttgct cagtctccac aattgtataa
      961 gcagatgtgc atttgtgctg attttgagaa ggttttctgc attggaccag ttttcagagc
     1021 tgaagactcc aatacccata gacatctgac tgaatttgtt ggtttggata ttgaaatggc
     1081 ctttaattat cattaccatg aagttgtaga agagattgct gacactttgg tgcaaatatt
     1141 caaaggactt caagaaaggt ttcagactga aattcaaact gtgagtaaac agttcccatg
     1201 tgagccgttc aaatttttgg agccaacttt aagactggag tattgcgagg ctttggcaat
     1261 gcttagagaa gctggagttg aaatggacga tgaggaagat ctaagtacac caaatgaaaa
     1321 actgttgggt cgtttggtga aggaaaagta tgatacagac ttttatgttc ttgataaata
     1381 tccattggct gtaagacctt tctataccat gcctgaccca agaaatccga aacagtccaa
     1441 ctcttacgat atgttcatga gaggagaaga aatattgtct ggagcacaaa gaatccatga
     1501 tcctcagttg cttacagaga gagctttaca ccatggaatt gatttggaga aaatcaaagc
     1561 ttacattgat tccttccgct ttggagctcc tcctcatgct ggtggtggca ttgggctgga
     1621 aagggtgacg atgctgtttt taggattgca taacgtacgc cagacctcaa tgttccctcg
     1681 tgaccccaaa cgactcacgc cttaaagcca cactttttat tctctccagg aacctgctat
     1741 tgattaggtt atacctgagg tacttaaaat atgcagtaga attaatgtct tactaaagtg
     1801 ccacaattat tttttaagta gctcagtaca aattaattta agtaaaaaac gttttagaat
     1861 ttcaaatatc ctaaaatagg aaatacttga acgttttaat gagaaatttc tatggttgaa
     1921 attcatagcc tttcactata ggaaatgtta agaaaaattt aaacagttta agattatata
     1981 attttttttt cttttctata ttgaggtggc caccatacta atattctaaa ttttcaatat
     2041 ttgaactttt aactcttgac attgaaaaaa aaggatttgg agaaattacc aagattttaa
     2101 catctataaa attggtatgt cttaaaagtt atttcaaaaa tcctgtgaag catctgaaac
     2161 tggctagaac cagtaacact gagacattgt taccagtcct gaggctgaga ttcaataaaa
     2221 acttacagct gaaaaaaaaa aaaaaaaaa
//
