LOCUS       NM_010012               1950 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus cytochrome P450, family 8, subfamily b, polypeptide 1
            (Cyp8b1), mRNA.
ACCESSION   NM_010012
VERSION     NM_010012.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   O'Leary,C.E., Sbierski-Kind,J., Kotas,M.E., Wagner,J.C.,
            Liang,H.E., Schroeder,A.W., de Tenorio,J.C., von Moltke,J.,
            Ricardo-Gonzalez,R.R., Eckalbar,W.L., Molofsky,A.B., Schneider,C.
            and Locksley,R.M.
  TITLE     Bile acid-sensitive tuft cells regulate biliary neutrophil influx
  JOURNAL   Sci Immunol 7 (69), eabj1080 (2022)
   PUBMED   35245089
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Aguirre,A.M., Yalcinkaya,N., Wu,Q., Swennes,A., Tessier,M.E.,
            Roberts,P., Miyajima,F., Savidge,T. and Sorg,J.A.
  TITLE     Bile acid-independent protection against Clostridioides difficile
            infection
  JOURNAL   PLoS Pathog 17 (10), e1010015 (2021)
   PUBMED   34665847
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1950)
  AUTHORS   Axling,U., Cavalera,M., Degerman,E., Gafvels,M., Eggertsen,G. and
            Holm,C.
  TITLE     Increased whole body energy expenditure and protection against
            diet-induced obesity in Cyp8b1-deficient mice is accompanied by
            altered adipose tissue features
  JOURNAL   Adipocyte 9 (1), 587-599 (2020)
   PUBMED   33016185
  REMARK    GeneRIF: Increased whole body energy expenditure and protection
            against diet-induced obesity in Cyp8b1-deficient mice is
            accompanied by altered adipose tissue features.
REFERENCE   4  (bases 1 to 1950)
  AUTHORS   Patankar,J.V., Wong,C.K., Morampudi,V., Gibson,W.T., Vallance,B.,
            Ioannou,G.N. and Hayden,M.R.
  TITLE     Genetic ablation of Cyp8b1 preserves host metabolic function by
            repressing steatohepatitis and altering gut microbiota composition
  JOURNAL   Am J Physiol Endocrinol Metab 314 (5), E418-E432 (2018)
   PUBMED   29066462
  REMARK    GeneRIF: The altered bile acid composition of Cyp8b1(-/-) mice
            preserves metabolic and respiratory function by repressing hepatic
            De Novo lipogenesis and driving favorable changes in gut
            antimicrobial responses.
REFERENCE   5  (bases 1 to 1950)
  AUTHORS   Bertaggia,E., Jensen,K.K., Castro-Perez,J., Xu,Y., Di Paolo,G.,
            Chan,R.B., Wang,L. and Haeusler,R.A.
  TITLE     Cyp8b1 ablation prevents Western diet-induced weight gain and
            hepatic steatosis because of impaired fat absorption
  JOURNAL   Am J Physiol Endocrinol Metab 313 (2), E121-E133 (2017)
   PUBMED   28377401
  REMARK    GeneRIF: Cyp8b1(-/-) mice were resistant to Western diet-induced
            body weight gain, hepatic steatosis, and insulin resistance.
REFERENCE   6  (bases 1 to 1950)
  AUTHORS   Murphy,C., Parini,P., Wang,J., Bjorkhem,I., Eggertsen,G. and
            Gafvels,M.
  TITLE     Cholic acid as key regulator of cholesterol synthesis, intestinal
            absorption and hepatic storage in mice
  JOURNAL   Biochim Biophys Acta 1735 (3), 167-175 (2005)
   PUBMED   15994119
REFERENCE   7  (bases 1 to 1950)
  AUTHORS   Choudhary,D., Jansson,I., Schenkman,J.B., Sarfarazi,M. and
            Stoilov,I.
  TITLE     Comparative expression profiling of 40 mouse cytochrome P450 genes
            in embryonic and adult tissues
  JOURNAL   Arch Biochem Biophys 414 (1), 91-100 (2003)
   PUBMED   12745259
REFERENCE   8  (bases 1 to 1950)
  AUTHORS   Li-Hawkins,J., Gafvels,M., Olin,M., Lund,E.G., Andersson,U.,
            Schuster,G., Bjorkhem,I., Russell,D.W. and Eggertsen,G.
  TITLE     Cholic acid mediates negative feedback regulation of bile acid
            synthesis in mice
  JOURNAL   J Clin Invest 110 (8), 1191-1200 (2002)
   PUBMED   12393855
  REMARK    GeneRIF: Feedback regulation of the rate-limiting biosynthetic
            enzyme CYP7A1 is lost in Cyp8b1(-/-) mice, causing expansion of the
            bile acid pool and alterations in cholesterol metabolism.
REFERENCE   9  (bases 1 to 1950)
  AUTHORS   Phan,J., Pesaran,T., Davis,R.C. and Reue,K.
  TITLE     The Diet1 locus confers protection against hypercholesterolemia
            through enhanced bile acid metabolism
  JOURNAL   J Biol Chem 277 (1), 469-477 (2002)
   PUBMED   11682476
REFERENCE   10 (bases 1 to 1950)
  AUTHORS   Gafvels,M., Olin,M., Chowdhary,B.P., Raudsepp,T., Andersson,U.,
            Persson,B., Jansson,M., Bjorkhem,I. and Eggertsen,G.
  TITLE     Structure and chromosomal assignment of the sterol
            12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic
            cytochrome P-450 gene devoid of introns
  JOURNAL   Genomics 56 (2), 184-196 (1999)
   PUBMED   10051404
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164092.3.
            
            On Apr 25, 2009 this sequence version replaced NM_010012.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC010973.2, AF090317.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1950              AC164092.3         122231-124180
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 72.73 cM"
     gene            1..1950
                     /gene="Cyp8b1"
                     /note="cytochrome P450, family 8, subfamily b, polypeptide
                     1"
                     /db_xref="GeneID:13124"
                     /db_xref="MGI:MGI:1338044"
     exon            1..1950
                     /gene="Cyp8b1"
                     /inference="alignment:Splign:2.1.0"
     CDS             42..1544
                     /gene="Cyp8b1"
                     /EC_number="1.14.14.139"
                     /note="CYPVIIIB1; cytochrome P450 8B1; sterol
                     12-alpha-hydroxylase; 7-alpha-hydroxy-4-cholesten-3-one
                     12-alpha-hydroxylase; sterol 12-alpha-hydrolase;
                     cytochrome P450, 8b1, sterol 12 alpha-hydrolase"
                     /codon_start=1
                     /product="7-alpha-hydroxycholest-4-en-3-one
                     12-alpha-hydroxylase"
                     /protein_id="NP_034142.3"
                     /db_xref="CCDS:CCDS23641.1"
                     /db_xref="GeneID:13124"
                     /db_xref="MGI:MGI:1338044"
                     /translation="MTLWCTVLGALLTVVGCLCLSLLLRHRRPWEPPLDKGFVPWLGH
                     SMAFRKNMFEFLKGMRAKHGDVFTVQLGGQYFTFVMDPLSFGPIIKNTEKALDFQSYA
                     KELVLKVFGYQSVDGDHRMIHLASTKHLMGQGLEELNQAMLDSLSLVMLGPKGSSLGA
                     SSWCEDGLFHFCYRILFKAGFLSLFGYTKDKQQDLDEADELFRKFRRFDFLFPRFVYS
                     LLGPREWVEVSQLQRLFHQRLSVKQNLEKDGISCWLGYMLQFLREQGIASSMQDKFNF
                     MMLWASQGNTGPTCFWVLLFLLKHQDAMKAVREEATRVMGKARLEAKKSFTFTPSALK
                     HTPVLDSVMEESLRLCATPTLLRVVQEDYVLKMASGQEYQIRRGDKVALFPYLSVHMD
                     PDIHPEPTAFKYDRFLNPDGTRKVDFYKSGKKIHHYSMPWGSGVSKCPGRFFALSEMK
                     TFVLLMIMYFDFKLVDPDIPVPPIDPRRWGFGTSQPSHEVRFLYRLKPVQ"
     regulatory      1927..1932
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp8b1"
                     /note="hexamer: TATAAA"
     polyA_site      1950
                     /gene="Cyp8b1"
                     /note="major polyA site"
ORIGIN      
        1 gtagagctga caagtggagc tcagcctgct ggagcctagc catgacgctg tggtgtacag
       61 tgctaggagc cctgctcacg gttgtgggat gcctgtgtct gtccttgctg ctcaggcacc
      121 gcaggccctg ggagccacct ctggacaagg gttttgtgcc ctggctgggt cactccatgg
      181 ctttccggaa gaatatgttt gaattcttga aggggatgcg ggccaagcac ggggatgtct
      241 tcacggtgca gctgggaggc cagtacttca cctttgtcat ggaccccctg tcctttggcc
      301 ccatcattaa gaacacagag aaagcgctgg acttccagtc ctatgcaaaa gaactggtgc
      361 tcaaggtgtt tgggtaccag tctgtagatg gggatcacag gatgatccac ttagctagta
      421 ccaagcatct gatggggcaa ggcttggagg aactcaacca ggccatgctg gacagcctat
      481 ccttggtgat gctagggcct aaaggttcga gtctgggtgc cagctcctgg tgtgaagatg
      541 gcctctttca cttctgctac agaatcctgt ttaaggctgg cttcctgagc ttattcggct
      601 acaccaagga caagcagcaa gacctggatg aggccgatga gctgttcagg aagttccgtc
      661 gatttgactt tctcttcccc aggtttgtct actccctact ggggccccgg gagtgggtgg
      721 aagtgagcca gttgcagcgt ctcttccatc agagactctc tgtgaagcaa aacctggaaa
      781 aagacggcat aagctgttgg ttaggctaca tgcttcagtt tctgagggag caaggaatag
      841 cttcatccat gcaggacaaa tttaacttta tgatgctctg ggcctcccag gggaacacgg
      901 gacccacctg tttctgggtc ctcttattcc tgctgaagca ccaggatgcc atgaaggctg
      961 tgcgtgagga agccaccagg gtcatgggta aggccaggct ggaagccaag aagtcgttca
     1021 ctttcactcc cagtgccctg aaacacactc cagtgttgga cagcgtgatg gaggagagtc
     1081 tgcgtctctg tgccaccccc accctcctca gggtggtaca ggaggattat gtcctgaaga
     1141 tggccagcgg acaagagtac cagatccgtc gcggagataa ggtggctctc ttcccctatc
     1201 tctcagtaca catggacccc gacatccacc ctgagcccac agccttcaag tatgatcggt
     1261 tcctcaaccc ggatggcacc cggaaagtgg acttctacaa gtcgggcaag aagatccacc
     1321 actacagcat gccttggggc tcgggtgttt ccaagtgccc cggcaggttc tttgccctca
     1381 gcgagatgaa gacctttgtc cttcttatga ttatgtactt cgacttcaag ctggtcgacc
     1441 ccgacatacc tgtgcccccc attgacccaa ggcgctgggg ctttggcacc tcacagccta
     1501 gccatgaggt acgcttcctc tatcgcctga agcccgtgca gtgagctcag gtctgagcat
     1561 accctgcagc ccagagcagc actgaatacc catcctctgg ctctgaggca ctccttcctc
     1621 tccccagctc tcagagatct gtcatgctcc ccataagacg ccatccctcc agcggtctct
     1681 gcctgaccct cctctcagga ggaacacatg tggtcagctc agtttacaac tgatggctca
     1741 cccacacttg actcatcctg gagggcagac cagctcccgt gagtcagatg gcatcttgca
     1801 aatgctgcct caacccatcc agcctgcctt actcgatgcc cttactccaa atcctaccag
     1861 ctcagactcc agggatgttg ctcaatggaa tcgagccatg tgtgcgactg ttaatttccc
     1921 actagatata aaagcaaggg attacgttga
//
