LOCUS       NM_007825               2135 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus cytochrome P450, family 7, subfamily b, polypeptide 1
            (Cyp7b1), transcript variant 1, mRNA.
ACCESSION   NM_007825
VERSION     NM_007825.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Chen,K.Y., De Giovanni,M., Xu,Y., An,J., Kirthivasan,N., Lu,E.,
            Jiang,K., Brooks,S., Ranucci,S., Yang,J., Kanameishi,S.,
            Kabashima,K., Brulois,K., Bscheider,M., Butcher,E.C. and
            Cyster,J.G.
  TITLE     Inflammation switches the chemoattractant requirements for naive
            lymphocyte entry into lymph nodes
  JOURNAL   Cell (2024) In press
   PUBMED   39708807
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Zhang,Z., Su,Z., Li,Z., Li,J., Yu,W., Ye,G., Lin,J., Che,Y., Xu,P.,
            Zeng,Y., Wu,Y., Shen,H. and Xie,Z.
  TITLE     CYP7B1-mediated 25-hydroxycholesterol degradation maintains
            quiescence-activation balance and improves therapeutic potential of
            mesenchymal stem cells
  JOURNAL   Cell Chem Biol 31 (7), 1277-1289 (2024)
   PUBMED   38382532
  REMARK    GeneRIF: CYP7B1-mediated 25-hydroxycholesterol degradation
            maintains quiescence-activation balance and improves therapeutic
            potential of mesenchymal stem cells.
REFERENCE   3  (bases 1 to 2135)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2135)
  AUTHORS   Goikolea,J., Latorre-Leal,M., Tsagkogianni,C., Pikkupeura,S.,
            Gulyas,B., Cedazo-Minguez,A., Loera-Valencia,R., Bjorkhem,I.,
            Rodriguez Rodriguez,P. and Maioli,S.
  TITLE     Different effects of CYP27A1 and CYP7B1 on cognitive function: Two
            mouse models in comparison
  JOURNAL   J Steroid Biochem Mol Biol 234, 106387 (2023)
   PUBMED   37648096
  REMARK    GeneRIF: Different effects of CYP27A1 and CYP7B1 on cognitive
            function: Two mouse models in comparison.
REFERENCE   5  (bases 1 to 2135)
  AUTHORS   Kobayashi,K., Iwasa,K., Azuma-Suzuki,R., Kawauchi,T. and
            Nabeshima,Y.I.
  TITLE     Feto-maternal cholesterol transport regulated by beta-Klotho-FGF15
            axis is essential for fetal growth
  JOURNAL   Life Sci Alliance 6 (10), e202301916 (2023)
   PUBMED   37541847
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2135)
  AUTHORS   Lammert,F., Carey,M.C. and Paigen,B.
  TITLE     Chromosomal organization of candidate genes involved in cholesterol
            gallstone formation: a murine gallstone map
  JOURNAL   Gastroenterology 120 (1), 221-238 (2001)
   PUBMED   11208732
  REMARK    Review article
REFERENCE   7  (bases 1 to 2135)
  AUTHORS   Li-Hawkins,J., Lund,E.G., Turley,S.D. and Russell,D.W.
  TITLE     Disruption of the oxysterol 7alpha-hydroxylase gene in mice
  JOURNAL   J Biol Chem 275 (22), 16536-16542 (2000)
   PUBMED   10748048
REFERENCE   8  (bases 1 to 2135)
  AUTHORS   Li-Hawkins,J., Lund,E.G., Bronson,A.D. and Russell,D.W.
  TITLE     Expression cloning of an oxysterol 7alpha-hydroxylase selective for
            24-hydroxycholesterol
  JOURNAL   J Biol Chem 275 (22), 16543-16549 (2000)
   PUBMED   10748047
REFERENCE   9  (bases 1 to 2135)
  AUTHORS   Schwarz,M., Lund,E.G., Lathe,R., Bjorkhem,I. and Russell,D.W.
  TITLE     Identification and characterization of a mouse oxysterol
            7alpha-hydroxylase cDNA
  JOURNAL   J Biol Chem 272 (38), 23995-24001 (1997)
   PUBMED   9295351
REFERENCE   10 (bases 1 to 2135)
  AUTHORS   Stapleton,G., Steel,M., Richardson,M., Mason,J.O., Rose,K.A.,
            Morris,R.G. and Lathe,R.
  TITLE     A novel cytochrome P450 expressed primarily in brain
  JOURNAL   J Biol Chem 270 (50), 29739-29745 (1995)
   PUBMED   8530364
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161213.3 and AC100500.10.
            
            On Jul 25, 2022 this sequence version replaced NM_007825.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK140100.1, AK172152.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-214               AC161213.3         53239-53452         c
            215-351             AC100500.10        57239-57375
            352-942             AC100500.10        63282-63872
            943-1149            AC100500.10        64347-64553
            1150-1325           AC100500.10        81596-81771
            1326-2135           AC100500.10        88323-89132
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 4.98 cM"
     gene            1..2135
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /note="cytochrome P450, family 7, subfamily b, polypeptide
                     1"
                     /db_xref="GeneID:13123"
                     /db_xref="MGI:MGI:104978"
     exon            1..214
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             99..1622
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /EC_number="1.14.14.29"
                     /EC_number="1.14.14.26"
                     /note="isoform 1 is encoded by transcript variant 1;
                     25-hydroxycholesterol 7-alpha-hydroxylase; cytochrome P450
                     7B1; oxysterol 7-alpha-hydroxylase; hippocampal transcript
                     1 protein; cytochrome P450, 7b1; 3-hydroxysteroid 7-alpha
                     hydroxylase; 24-hydroxycholesterol 7-alpha-hydroxylase;
                     25/26-hydroxycholesterol 7-alpha-hydroxylase"
                     /codon_start=1
                     /product="cytochrome P450 7B1 isoform 1"
                     /protein_id="NP_031851.3"
                     /db_xref="CCDS:CCDS17254.1"
                     /db_xref="GeneID:13123"
                     /db_xref="MGI:MGI:104978"
                     /translation="MQGATTLDAASPGPLALLGLLFAATLLLSALFLLTRRTRRPREP
                     PLIKGWLPYLGMALKFFKDPLTFLKTLQRQHGDTFTVFLVGKYITFVLNPFQYQYVTK
                     NPKQLSFQKFSSRLSAKAFSVKKLLTDDDLNEDVHRAYLLLQGKPLDALLETMIQEVK
                     ELFESQLLKITDWNTERIFAFCGSLVFEITFATLYGKILAGNKKQIISELRDDFFKFD
                     DMFPYLVSDIPIQLLRNEESMQKKIIKCLTSEKVAQMQGQSKIVQERQDLLKRYYRHD
                     DPEIGAHHLGFLWASLANTIPAMFWAMYYILRHPEAMEALRDEIDSFLQSTGQKKGPG
                     ISVHFTREQLDSLVCLESTILEVLRLCSYSSIIREVQEDMNLSLESKSFSLRKGDFVA
                     LFPPLIHNDPEIFDAPKEFRFDRFIEDGKKKSTFFKGGKKLKTYVMPFGLGTSKCPGR
                     YFAVNEMKLLLIMLLTYFDLEIIDRKPIGLNHSRMFLGIQHPDSAVSFRYKAKSWRS"
     exon            215..351
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /inference="alignment:Splign:2.1.0"
     exon            352..942
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1149
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1325
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1326..2135
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1782..1787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /note="hexamer: AATAAA"
     polyA_site      1808
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /note="major polyA site"
     regulatory      1903..1908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /note="hexamer: AATAAA"
     polyA_site      1943
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
     regulatory      2114..2119
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
                     /note="hexamer: ATTAAA"
     polyA_site      2135
                     /gene="Cyp7b1"
                     /gene_synonym="D3Ertd552e; hct-1"
ORIGIN      
        1 attctgctct gtgcagcggg caggccacag cctctggtct agaagagagg gcactgtgca
       61 gaagccatcg ctccctacag agccgccagc tcgtcgggat gcagggagcc acgaccctag
      121 atgccgcctc gccagggcct ctcgccctcc taggccttct ctttgccgcc accttactgc
      181 tctcggccct gttcctcctt acccggcgca ccaggcgccc tcgtgaacca cccttgataa
      241 aaggttggct tccttatctt ggcatggccc tgaaattctt taaggatccg ttaactttct
      301 tgaaaactct tcaaaggcaa catggtgaca ctttcactgt cttccttgtg gggaagtata
      361 taacatttgt tctgaaccct ttccagtacc agtatgtaac gaaaaaccca aaacaattaa
      421 gctttcagaa gttcagcagc cgattatcag cgaaagcctt ctctgtaaag aagctgctta
      481 ctgatgacga ccttaatgaa gacgttcaca gagcctatct acttctacaa ggcaaacctt
      541 tggatgctct tctggaaact atgatccaag aagtaaaaga attatttgag tcccaactgc
      601 taaaaatcac agattggaac acagaaagaa tatttgcatt ctgtggctca ctggtatttg
      661 agatcacatt tgcgactcta tatggaaaaa ttcttgctgg taacaagaaa caaattatca
      721 gtgagctaag ggatgatttt tttaaatttg atgacatgtt cccatactta gtatctgaca
      781 tacctattca gcttctaaga aatgaagaat ctatgcagaa gaaaattata aaatgcctca
      841 catcagaaaa agtagctcag atgcaaggac agtcaaaaat tgttcaggaa aggcaagatc
      901 tgctgaaaag atactatagg catgacgatc ctgaaatagg agcacatcat cttggctttc
      961 tctgggcctc tctagcaaac accattccag ctatgttctg ggcaatgtat tatattcttc
     1021 ggcatcctga agctatggaa gccctgcgtg acgaaattga cagtttcctg cagtcaacag
     1081 gtcaaaagaa aggacctgga atttcagtcc acttcaccag agaacaattg gacagcttgg
     1141 tctgcctgga aagcactatt cttgaggttc tgaggctgtg ctcatactcc agcatcatcc
     1201 gagaagtgca ggaggatatg aatctcagct tagagagtaa gagtttctct ctgcggaaag
     1261 gagattttgt agccctcttt cctccactca tacacaatga cccggaaatc ttcgatgctc
     1321 caaaggaatt taggttcgat cgcttcatag aagatggtaa gaagaaaagc acgtttttca
     1381 aaggagggaa gaagctgaag acttacgtta tgccttttgg actcggaaca agcaaatgtc
     1441 cagggagata ttttgcagtg aacgaaatga agttactgct gattatgctt ttaacttatt
     1501 ttgatttaga aattatcgac aggaagccta tagggctaaa tcacagtcgg atgtttttag
     1561 gtattcagca ccccgattct gccgtctcct ttaggtacaa agcaaaatct tggagaagct
     1621 gaaagtgtgg cagagaagct ttgcagagta aggctgcatg tgctgagctc cgtgatttgg
     1681 tgcactcccc caaatgcaac cgctactctt gtttgaaaat ggcaaattta tatttggttg
     1741 agatcaatcc agttggtttt gggtcacaaa acctgtcata aaataaagca gtgtgatggt
     1801 ttaaaaaatg tcatggcaat catttcagga taaggtaaaa taacattttc aagtttgtac
     1861 ttactatgat ttttatcatt tgtagtgaat gtgcttttct gtaataaatt tgctccaggg
     1921 tgattttatt taagttactg aaatcctcta atatggtttt atgttctgcc agaaagctgt
     1981 gccttcaatg gacagtctaa caatttcccg ttttccagag aagggggaca ttaaatccca
     2041 tgaattacac tgtatgaaaa ttttcccttc aagtatagta tcaataacgt ctacatcacc
     2101 agggtacctt tgcattaaat gagttttgca aaata
//
