LOCUS       NM_007824               4173 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus cytochrome P450, family 7, subfamily a, polypeptide 1
            (Cyp7a1), mRNA.
ACCESSION   NM_007824
VERSION     NM_007824.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Chen,P.C., Hsu,C.P., Wang,S.Y., Wu,T.Y., Lin,Y.J., Chen,Y.T. and
            Hsu,S.H.
  TITLE     miR-194 Up-Regulates Cholesterol 7 Alpha-Hydroxylase Expression via
            beta-Catenin Signaling and Aggravates Cholestatic Liver Diseases
  JOURNAL   Am J Pathol 193 (6), 755-768 (2023)
   PUBMED   36868469
  REMARK    GeneRIF: miR-194 Up-Regulates Cholesterol 7 Alpha-Hydroxylase
            Expression via beta-Catenin Signaling and Aggravates Cholestatic
            Liver Diseases.
REFERENCE   3  (bases 1 to 4173)
  AUTHORS   Kouno,T., Liu,X., Zhao,H., Kisseleva,T., Cable,E.E. and Schnabl,B.
  TITLE     Selective PPARdelta agonist seladelpar suppresses bile acid
            synthesis by reducing hepatocyte CYP7A1 via the fibroblast growth
            factor 21 signaling pathway
  JOURNAL   J Biol Chem 298 (7), 102056 (2022)
   PUBMED   35605662
  REMARK    GeneRIF: Selective PPARdelta agonist seladelpar suppresses bile
            acid synthesis by reducing hepatocyte CYP7A1 via the fibroblast
            growth factor 21 signaling pathway.
REFERENCE   4  (bases 1 to 4173)
  AUTHORS   Zhang,J., Wang,X., Jiang,H., Yang,F., Du,Y., Wang,L. and Hong,B.
  TITLE     MicroRNA-185 modulates CYP7A1 mediated cholesterol-bile acid
            metabolism through post-transcriptional and post-translational
            regulation of FoxO1
  JOURNAL   Atherosclerosis 348, 56-67 (2022)
   PUBMED   35287950
  REMARK    GeneRIF: MicroRNA-185 modulates CYP7A1 mediated cholesterol-bile
            acid metabolism through post-transcriptional and post-translational
            regulation of FoxO1.
REFERENCE   5  (bases 1 to 4173)
  AUTHORS   Pantasis,S., Friemel,J., Brutsch,S.M., Hu,Z., Krautbauer,S.,
            Liebisch,G., Dengjel,J., Weber,A., Werner,S. and Bordoli,M.R.
  TITLE     Vertebrate lonesome kinase modulates the hepatocyte secretome to
            prevent perivascular liver fibrosis and inflammation
  JOURNAL   J Cell Sci 135 (7) (2022)
   PUBMED   35293576
REFERENCE   6  (bases 1 to 4173)
  AUTHORS   Agellon,L.B. and Cheema,S.K.
  TITLE     The 3'-untranslated region of the mouse cholesterol
            7alpha-hydroxylase mRNA contains elements responsive to
            post-transcriptional regulation by bile acids
  JOURNAL   Biochem J 328 (Pt 2) (Pt 2), 393-399 (1997)
   PUBMED   9371693
REFERENCE   7  (bases 1 to 4173)
  AUTHORS   Machleder,D., Ivandic,B., Welch,C., Castellani,L., Reue,K. and
            Lusis,A.J.
  TITLE     Complex genetic control of HDL levels in mice in response to an
            atherogenic diet. Coordinate regulation of HDL levels and bile acid
            metabolism
  JOURNAL   J Clin Invest 99 (6), 1406-1419 (1997)
   PUBMED   9077551
REFERENCE   8  (bases 1 to 4173)
  AUTHORS   Schwarz,M., Lund,E.G., Setchell,K.D., Kayden,H.J., Zerwekh,J.E.,
            Bjorkhem,I., Herz,J. and Russell,D.W.
  TITLE     Disruption of cholesterol 7alpha-hydroxylase gene in mice. II. Bile
            acid deficiency is overcome by induction of oxysterol
            7alpha-hydroxylase
  JOURNAL   J Biol Chem 271 (30), 18024-18031 (1996)
   PUBMED   8663430
REFERENCE   9  (bases 1 to 4173)
  AUTHORS   Ishibashi,S., Schwarz,M., Frykman,P.K., Herz,J. and Russell,D.W.
  TITLE     Disruption of cholesterol 7alpha-hydroxylase gene in mice. I.
            Postnatal lethality reversed by bile acid and vitamin
            supplementation
  JOURNAL   J Biol Chem 271 (30), 18017-18023 (1996)
   PUBMED   8663429
REFERENCE   10 (bases 1 to 4173)
  AUTHORS   Tzung,K.W., Ishimura-Oka,K., Kihara,S., Oka,K. and Chan,L.
  TITLE     Structure of the mouse cholesterol 7 alpha-hydroxylase gene
  JOURNAL   Genomics 21 (1), 244-247 (1994)
   PUBMED   8088795
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK050210.1 and AK050260.1.
            
            On Apr 27, 2018 this sequence version replaced NM_007824.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK050260.1, AK050020.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AK050210.1         1-1
            2-4173              AK050260.1         1-4172
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 2.91 cM"
     gene            1..4173
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /note="cytochrome P450, family 7, subfamily a, polypeptide
                     1"
                     /db_xref="GeneID:13122"
                     /db_xref="MGI:MGI:106091"
     exon            1..141
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1573
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /EC_number="1.14.14.23"
                     /EC_number="1.14.14.26"
                     /note="cholesterol 7-alpha-monooxygenase; cholesterol
                     7-alpha-hydroxylase; cytochrome P450, 7a1;
                     24-hydroxycholesterol 7-alpha-hydroxylase"
                     /codon_start=1
                     /product="cytochrome P450 7A1"
                     /protein_id="NP_031850.2"
                     /db_xref="CCDS:CCDS17950.1"
                     /db_xref="GeneID:13122"
                     /db_xref="MGI:MGI:106091"
                     /translation="MMSISLIWGIAVVVSCCIWFIIGIRRRKVGEPPLDNGLIPYLGC
                     ALKFGSNPLEFLRAKQRKHGHVFTCKLMGKYVHFITNSLSYHKVLCHGKYFDWKKFHY
                     TTSAKAFGHRSIDPSDGNTTENINKTFNKTLQGDALCSLSEAMMQNLQSVMRPPGLPK
                     SKSAVWVTEGMYAFCYRVMFEAGYLTLFGKDISKTDSQRAFIQNNLDSFKQFDQVFPA
                     LVAGVPIHLFKTAHKARERLAESLKHKNLYMRDQVSELIRLRMFLNDTLSTFDDMEKA
                     KTHLVILWASQANTIPATFWSLFQMIRSPEAMKAASEEVNGALQSAGQELSSGGNAIY
                     LDQEQLNNLPVLDSIIKEALRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQ
                     LMHLDPEIYPDPLTFKYDRYLDESGKAKTTFYRNGNKLKYFYMPFGSGATICPGRLFA
                     VQEIKQFLILMLSYFELELVESHTKCPPLDQSRAGLGILPPLNDIEFKYKLKH"
     exon            142..382
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /inference="alignment:Splign:2.1.0"
     exon            383..969
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1100
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1276
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..4173
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3724..3729
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /note="hexamer: ACTAAA"
     polyA_site      3747
                     /gene="Cyp7a1"
                     /gene_synonym="CYPVII; CYPVIIc"
                     /note="major polyA site"
ORIGIN      
        1 ggaccgtctt gctttgctaa gcacagattc tccccttggg acgttttcct gcttttgcaa
       61 aatgatgagc atttctttga tctgggggat tgctgtggta gtgagctgtt gcatatggtt
      121 tatcattgga ataaggagaa ggaaagtagg tgaacctcct ttggacaacg ggttgattcc
      181 atacctgggc tgtgctctga agttcggatc caatcctctt gaattcctaa gagcaaagca
      241 aaggaaacat ggccatgttt ttacctgcaa actgatgggg aaatatgtcc acttcatcac
      301 aaactccctg tcataccaca aagtcttatg tcacggaaaa tattttgact ggaaaaaatt
      361 tcattacact acttctgcga aggcatttgg acacagaagc atagacccaa gtgatggaaa
      421 taccacggaa aacataaaca agacttttaa caaaaccctc cagggagatg ctctgtgttc
      481 actctctgaa gccatgatgc aaaacctcca atctgtcatg agacctccgg gccttcctaa
      541 atcaaagagc gctgtctggg tcacggaagg gatgtatgcc ttctgctacc gagtgatgtt
      601 tgaagccgga tatctaacgc tgtttggcaa agatatttca aagacagact cacaaagagc
      661 atttattcaa aacaaccttg acagcttcaa acaatttgac caagtatttc cggcactagt
      721 ggcgggcgtc cctattcact tgttcaagac cgcacataaa gcccgggaaa ggctggctga
      781 gagcttgaag cacaagaacc tgtacatgag ggaccaggtc tctgaactga tccgtctacg
      841 catgtttctc aacgatacac tctccacctt tgatgacatg gagaaggcta agacgcacct
      901 cgtgatcctc tgggcatctc aagcaaacac cattcctgca accttctgga gcttatttca
      961 aatgatcagg agccctgaag caatgaaagc agcctctgaa gaagtgaatg gagcattaca
     1021 gagtgctggc caagagctca gctctggagg gaatgccatt tacttggatc aagagcaact
     1081 aaacaacctg ccagtactag atagcatcat caaggaggct ctgcggctct ccagtgcatc
     1141 cttgaatatc cggacagcta aggaggactt cactctacac cttgaggatg gttcctataa
     1201 cattcgaaaa gatgacatca tagctcttta cccacagtta atgcacttgg atcctgaaat
     1261 ctacccagac cctttgactt ttaaatacga ccggtacctt gatgaaagtg ggaaagcaaa
     1321 gaccaccttc tatagaaatg gaaacaagct gaagtatttc tacatgccct ttggatcagg
     1381 agctacaata tgtcctggaa gactatttgc tgtccaagaa atcaagcaat ttttgattct
     1441 gatgctgtct tactttgaac tggagcttgt agagagccac accaagtgtc cccctctaga
     1501 tcagtcccgg gcaggcttgg gaattttgcc accactaaat gatattgagt ttaaatataa
     1561 actgaaacac tgatatgtgg ttggaagaag aggacactgg atgatgtaac gactgctgag
     1621 cgttatcagt aaacaggcct ttgggactag tgctcactga agccccctag tagctgtatt
     1681 agtgagaaga actctgttct tactgctcac gttcctgggg attcgtgtag ctggggcctg
     1741 agtttcatca ctttcagagc aacgtctttt gtttttattt tcaaaatgaa gatattccaa
     1801 ttggcagggt tctttcctaa ggaaattgct ttatattttt atgaaaacta ccgattaatt
     1861 atggaagtac ttcaaattca cgttttagtg aaattattaa tttttcacta gtaaggttct
     1921 tcatgtgtga gtatattata aaaatgtttt agctgatcat atcatgcttt gcataaaggg
     1981 aaaggaaatt attgttcagc ttttttttta tggtggtgag agcttgaaaa tgatcttact
     2041 attctagaac tactagggaa gtttcgacat gctctcgcta tattttaatt tattgttgct
     2101 ggagattttt attccagttt tgaactactt tatctttcct tctttttgac acacatacca
     2161 ataagaagag catttttcag aaattataaa ggcacctcca agaaccacac catgagtctt
     2221 ttaagccttt aattccagct cccatgaggc aaagccaggc aaatctctat gagtctgagg
     2281 ttattctggt ctacatcagc tctagacaag ccaggactac agaaaggacc ttgtctaaaa
     2341 agaaaaaaag ttaatttcta tgtcatattt gattatgaat caacatgaaa tataaattta
     2401 aaatcaggac tcagagaaat gatcaattaa aaaacttagc tatgaagtat gtggagttct
     2461 ttaagtacaa gttgacatta tatgttcttt ttttatggtt tgttgtagaa aggcacggag
     2521 aaagaaagaa ggtagaaaga gagagacacc ggccatggcc acgtggagag aggggggaag
     2581 ggaaagagag aaggaggcct agagagtaag aaaggtgagg gcttaaagag ctatatgctc
     2641 tttaaaaatg ttttatgttt ttatctctaa atgccttaaa gatgaagaac aataatgaaa
     2701 ggctgagtaa tagtgtttaa atactgagtg caatagtgct ttagtaatat actttaaaga
     2761 gagttattag ctatgtcatt tttactgaaa atatatttat atataaatta tatttatctt
     2821 tttcttatac catatatata aaaatattgt aacatttagt aattttaaaa ttacacacct
     2881 ttcagaaatt aacatatgaa tgttcgtgtt ttaaactttg aacagaacat ttaaattatt
     2941 catctactgg taagctacaa taatttttct catatttatt taaaatattc atattttcaa
     3001 gaaatccaaa aatatccaaa gtaatcactc agtcaaatag atccctaaga tgacagtaaa
     3061 ttcaatacca tgcttttgtc tgcgagggct ggagcagggt ccagtggatg ctgtgcaggt
     3121 cctccagcag agagctaaat ccacagactc ctcaaaatct taaaacctta ccactgaata
     3181 ctggcacaga gttattacct aggtacgcta tgcttccttc ctttttcatt ttgacagagg
     3241 cccatagcat cccaggctgg aacattccgc gtagcttaag caagcttgga actcactgtg
     3301 cttcctgctt tgccttgtta taggaagtaa ttttcaagac caagaaaact taattgttta
     3361 gatgagctat aagaaagaca caattgtgtt gtatactaat ctgtactaag ctaaatttgt
     3421 ttttaaaaca agttttatga ctccctgaac tgaatgtatc cacatacctt cccatggctt
     3481 tttaaaagca tatttttcat acagaatgat gggtctcctg gtggtgcctt cttctcatat
     3541 ttttattctt gtgaagtgat ggctagcaaa caatagcctg tcaaccaaag ttactcttcc
     3601 cgtttctgtg cagcctaagt gttaaaagta atttttacat ttttaaatgc ttaggggaaa
     3661 aaaaccacat gtctattttg tgtcatatac gaagtatatg aaatacagac tagcaaaccc
     3721 gtgactaaaa cttttattgg gatatcagaa tctgcctaat tctttgcgtt atgtctacaa
     3781 ttccattttt tgacatgtgt atgtagtgtg catttttgtg gaagcatgtg tgagtgtgtg
     3841 tgcacatgtg ggtaaacatg tgtgcatgtg tgtagaggct ggaggtgatg ttgagtgttt
     3901 tcctccactg ttctcccttg agggtctctc ccttgagcca gagtccaatg cttaggctag
     3961 catagccaac ttgccgcagt gcccttgaat tgcaggaggt tgccacatcc tcctggcatt
     4021 taccctggtg tgagatgagc ttcagaaggg caagtgcttt gtctgctagt ctcagtgtct
     4081 acagctcttg ctgtgtaatt gtaaaggcag ggtagtgtga catagacagg aggacacaga
     4141 gcttaccatt tcaaaaatca gtttcttcag agt
//
