LOCUS       NM_177307               2618 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cytochrome P450, family 4, subfamily f, polypeptide 39
            (Cyp4f39), mRNA.
ACCESSION   NM_177307
VERSION     NM_177307.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Ohno,Y., Nakamura,T., Iwasaki,T., Katsuyama,A., Ichikawa,S. and
            Kihara,A.
  TITLE     Determining the structure of protein-bound ceramides, essential
            lipids for skin barrier function
  JOURNAL   iScience 26 (11), 108248 (2023)
   PUBMED   37965138
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2618)
  AUTHORS   Kato,A., Ito,M., Sanaki,T., Okuda,T., Tsuchiya,N., Yoshimoto,R. and
            Yukioka,H.
  TITLE     Acsl1 is essential for skin barrier function through the activation
            of linoleic acid and biosynthesis of omega-O-acylceramide in mice
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1867 (2), 159085 (2022)
   PUBMED   34813948
REFERENCE   4  (bases 1 to 2618)
  AUTHORS   Sawai,M., Watanabe,K., Tanaka,K., Kinoshita,W., Otsuka,K.,
            Miyamoto,M., Sassa,T. and Kihara,A.
  TITLE     Diverse meibum lipids produced by Awat1 and Awat2 are important for
            stabilizing tear film and protecting the ocular surface
  JOURNAL   iScience 24 (5), 102478 (2021)
   PUBMED   34113821
  REMARK    Erratum:[iScience. 2021 Sep 25;24(10):103160. PMID: 34622178]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2618)
  AUTHORS   Miyamoto,M., Sassa,T., Sawai,M. and Kihara,A.
  TITLE     Lipid polarity gradient formed by omega-hydroxy lipids in tear film
            prevents dry eye disease
  JOURNAL   Elife 9, e53582 (2020)
   PUBMED   32252890
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2618)
  AUTHORS   Miyamoto,M., Itoh,N., Sawai,M., Sassa,T. and Kihara,A.
  TITLE     Severe Skin Permeability Barrier Dysfunction in Knockout Mice
            Deficient in a Fatty Acid omega-Hydroxylase Crucial to Acylceramide
            Production
  JOURNAL   J Invest Dermatol 140 (2), 319-326 (2020)
   PUBMED   31356814
  REMARK    GeneRIF: Severe Skin Permeability Barrier Dysfunction in Knockout
            Mice Deficient in a Fatty Acid omega-Hydroxylase Crucial to
            Acylceramide Production.
REFERENCE   7  (bases 1 to 2618)
  AUTHORS   Pichery,M., Huchenq,A., Sandhoff,R., Severino-Freire,M.,
            Zaafouri,S., Opalka,L., Levade,T., Soldan,V., Bertrand-Michel,J.,
            Lhuillier,E., Serre,G., Maruani,A., Mazereeuw-Hautier,J. and
            Jonca,N.
  TITLE     PNPLA1 defects in patients with autosomal recessive congenital
            ichthyosis and KO mice sustain PNPLA1 irreplaceable function in
            epidermal omega-O-acylceramide synthesis and skin permeability
            barrier
  JOURNAL   Hum Mol Genet 26 (10), 1787-1800 (2017)
   PUBMED   28369476
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK037192.1.
            
            On Apr 6, 2007 this sequence version replaced NM_177307.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK037192.1, AK028950.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849387, SAMN01164133
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2618              AK037192.1         2-2619
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 17.59 cM"
     gene            1..2618
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /note="cytochrome P450, family 4, subfamily f, polypeptide
                     39"
                     /db_xref="GeneID:320997"
                     /db_xref="MGI:MGI:2445210"
     exon            1..74
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            75..177
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            178..400
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..1777
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /EC_number="1.14.14.94"
                     /codon_start=1
                     /product="cytochrome P450, family 2, subfamily E,
                     polypeptide 2 homolog"
                     /protein_id="NP_796281.1"
                     /db_xref="CCDS:CCDS28618.1"
                     /db_xref="GeneID:320997"
                     /db_xref="MGI:MGI:2445210"
                     /translation="MLPLTDYLLQLLGLEKTAFRVYVVSALLLLVLFFFFRLLVRAFK
                     LFSDFRITCRKLSCFPEPPGRHWLLGHMSMYLPNEKGLQNEKKVLDTMHHIILAWVGP
                     FLPLLVLVHPDYIKPVLGASAAIAPKDEFFYSFLKPWLGDGLLISKGNKWSRHRRLLT
                     PAFHFDILKPYMKIFNQCTNIMHAKWRRHLAEGSVTSFDMFEHISLMTLDSLQKCVFS
                     YNSDCQERMSDYISSIIELSALVVRRQYRLHHYLDFMYYLTADGRRFRQACDTVHNFT
                     TEVIQERRQALRQQGAEAWLKAKQGKTLDFIDVLLLAKDEEGKELSDEDIRAEADTFM
                     FEGHDTTSSGLSWALFNLAKYPEYQEKCREEIQEVMKGRELEELDWDDLTQLPFTTMC
                     IKESLRQFPPVTLISRRCTEDIKLPDGRVIPKGIICLVSIYGTHHNPIVWPDSKVYNP
                     YRFDPDTPQQRSPLAFVPFSAGPRNCIGQSFAMAEMRVVVALTLLRFRLSVDRTHKVR
                     RKPELILRTENGLWLNVEPLPSRA"
     exon            401..545
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            546..599
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            600..727
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            728..852
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            853..1120
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1187
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1317
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1318..1451
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1516
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1599
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1600..2618
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2429..2434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /note="hexamer: AGTAAA"
     polyA_site      2450
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
     regulatory      2550..2555
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /note="hexamer: ACTAAA"
     polyA_site      2577
                     /gene="Cyp4f39"
                     /gene_synonym="4732474A20Rik"
                     /note="major polyA site"
ORIGIN      
        1 ttaaagggtt ggggctgtca gactgcactg tcaaagaagc ggatctggat cgagagcggc
       61 aggctgagac ccgggagctg gcccaaagtc aaggatctga gtcccgacaa cttctctgag
      121 aagcagtttc agcaaatacc agtaaagaaa gaagaaagcc aagagaattg aaagcaggat
      181 gctgcccctc accgactacc tgttgcagct tctggggctg gagaagacag cctttcgagt
      241 gtatgttgtg tcggctctgc tgctattggt ccttttcttc ttcttccgcc tgctggtgcg
      301 ggcattcaaa ttatttagtg acttccgcat tacctgtcgc aagctgagct gcttcccaga
      361 gcctccaggc cgccactggc tgctgggtca catgagcatg tatcttccca atgagaaggg
      421 ccttcaaaac gagaagaagg tgctggacac catgcaccac atcatcctgg catgggttgg
      481 acctttcttg cctctcctgg tgcttgttca tcctgattac atcaagcctg tgttgggtgc
      541 ctcagctgcc attgcaccca aggatgagtt tttctatagc ttcctgaagc cttggttagg
      601 ggatggactt ctgatcagca aagggaacaa atggagccgg caccgccgcc tgctcacacc
      661 tgccttccac tttgacatcc tgaagcccta catgaagatt ttcaaccagt gcacgaacat
      721 catgcacgct aaatggcggc gacatctggc agagggctca gtgacctcct ttgacatgtt
      781 cgagcacatc agccttatga ccctggacag ccttcagaaa tgtgtgttca gttacaacag
      841 tgactgccag gagagaatga gcgattacat ttcttccatt atcgaactga gcgctctggt
      901 ggtgcggcgc caataccgct tgcatcacta cctggatttc atgtactacc tcacagccga
      961 tgggaggcgc ttccgtcagg cctgcgacac ggtgcacaac ttcaccactg aagtcataca
     1021 ggagcggcgg caggcgctgc gccagcaggg cgccgaggca tggctgaaag ccaagcaggg
     1081 caagaccttg gacttcatag atgtgctgtt gctggccaag gatgaagagg ggaaagaatt
     1141 atctgacgag gacatccggg ccgaggcaga tactttcatg tttgaaggtc atgacacaac
     1201 atctagtggg ctgtcctggg cactgttcaa cctggcaaag tatccagagt accaggagaa
     1261 gtgccgggag gagatccaag aagtcatgaa aggcagagag ctggaagagc tggactggga
     1321 tgacctcact cagttgccct tcaccaccat gtgtatcaag gagagccttc gccagttccc
     1381 acctgtgacc ctcatctctc gtcgctgcac agaggacatc aagctgccag atggacgtgt
     1441 catccccaaa ggaattatct gcttggtcag catctacggg acccaccaca accccatagt
     1501 gtggcctgac tctaaggtat acaatcctta ccgttttgac ccggacaccc cacagcagcg
     1561 ctctccactg gcctttgtac ctttctcagc aggtcccagg aattgcatcg ggcagagctt
     1621 cgccatggca gagatgcgtg tagtcgtggc gctgacactg ctgcgcttcc gcctgagcgt
     1681 ggaccgaacc cacaaggtgc ggaggaagcc ggagcttatt ctacgcacgg agaacggcct
     1741 ttggctcaat gtagagcctc taccctcacg ggcctgagtg cgcccttgct ccgcggatcc
     1801 tgggattcag ggattcaggc tccgcccgct caggcctaga tcacgcctcc aatagcatgg
     1861 atccgccccc aagatattga gggcgttgac tacgctcctc gaaagtactg gaggacaaag
     1921 ctttgtttgt agaggagtat cttgttggtg ctgacttcgc ccctcaggac aagactccac
     1981 ccttgggatt tgattccacc ctctgaggtc gaaattacgc tttgaattat tgtcaccggc
     2041 cctcagcttg agggaatgat gctaggccac gccccactcg ggcctaggcc acgccccagt
     2101 cagattaccc tggccctgcg gattctgatt cctaggccca gctcacagaa acttggacct
     2161 aagactcacc actggggttg tatcctctgg ctttccctct gcctatgccc aagttagctt
     2221 ctaaatggag gatttagaag ccggagtctg aggttgcagt cctgaatctt caacatcctt
     2281 tcccaagacc cacagctcca tgaaaggaca gttcaagctc tcaggattta ggctgtttgc
     2341 tttgttgttt tgtaaacctg ggcccttgca agccccttcc ttcttggtca ccgtcccttt
     2401 gctgagtttt ctcaatttta agagtgagag taaaagcaag aggatccaaa caccgtccat
     2461 ctcatccggt cctagatttg gagcccaagg tggggaggtg ggcctcttga cagtcgggaa
     2521 gcaactggga catacttatt tgatctggaa ctaaactcag gttttttttt gggacaattt
     2581 gtgctccgtt tatttggtca atagattgca tattaatt
//
