LOCUS       NM_145499               1709 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus cytochrome P450, family 2, subfamily c, polypeptide 70
            (Cyp2c70), mRNA.
ACCESSION   NM_145499
VERSION     NM_145499.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Yntema,T., Eijgenraam,T.R., Kloosterhuis,N.J., Havinga,R.,
            Koster,M.H., Hovingh,M.V., de Boer,J.F., Koonen,D.P.Y. and
            Kuipers,F.
  TITLE     The impact of a humanized bile acid composition on atherosclerosis
            development in hypercholesterolaemic Cyp2c70 knockout mice
  JOURNAL   Sci Rep 15 (1), 2100 (2025)
   PUBMED   39815082
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Sjoland,W., Wahlstrom,A., Makki,K., Scholer,M., Molinaro,A.,
            Olsson,L., Greiner,T.U., Caesar,R., de Boer,J.F., Kuipers,F.,
            Backhed,F. and Marschall,H.U.
  TITLE     Absence of gut microbiota reduces neonatal survival and exacerbates
            liver disease in Cyp2c70-deficient mice with a human-like bile acid
            composition
  JOURNAL   Clin Sci (Lond) 137 (13), 995-1011 (2023)
   PUBMED   37384590
  REMARK    GeneRIF: Absence of gut microbiota reduces neonatal survival and
            exacerbates liver disease in Cyp2c70-deficient mice with a
            human-like bile acid composition.
REFERENCE   3  (bases 1 to 1709)
  AUTHORS   Hasan,M.N., Chen,J., Wang,H., Du,Y., Clayton,Y.D., Gu,L. and Li,T.
  TITLE     Glycine-beta-Muricholic Acid Improves Liver Fibrosis and Gut
            Barrier Function by Reducing Bile Acid Pool Size and Hydrophobicity
            in Male Cyp2c70 Knockout Mice
  JOURNAL   Cells 12 (10), 1371 (2023)
   PUBMED   37408204
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1709)
  AUTHORS   Truong,J.K., Bennett,A.L., Klindt,C., Donepudi,A.C., Malla,S.R.,
            Pachura,K.J., Zaufel,A., Moustafa,T., Dawson,P.A. and Karpen,S.J.
  TITLE     Ileal bile acid transporter inhibition in Cyp2c70 KO mice
            ameliorates cholestatic liver injury
  JOURNAL   J Lipid Res 63 (9), 100261 (2022)
   PUBMED   35934110
REFERENCE   5  (bases 1 to 1709)
  AUTHORS   Li,R., Hovingh,M.V., Koehorst,M., de Blaauw,P., Verkade,H.J., de
            Boer,J.F. and Kuipers,F.
  TITLE     Short-term obeticholic acid treatment does not impact
            cholangiopathy in Cyp2c70-deficient mice with a human-like bile
            acid composition
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1867 (8), 159163 (2022)
   PUBMED   35470044
REFERENCE   6  (bases 1 to 1709)
  AUTHORS   de Boer,J.F., Verkade,E., Mulder,N.L., de Vries,H.D., Huijkman,N.,
            Koehorst,M., Boer,T., Wolters,J.C., Bloks,V.W., van de Sluis,B. and
            Kuipers,F.
  TITLE     A human-like bile acid pool induced by deletion of hepatic Cyp2c70
            modulates effects of FXR activation in mice
  JOURNAL   J Lipid Res 61 (3), 291-305 (2020)
   PUBMED   31506275
  REMARK    GeneRIF: A human-like bile acid pool induced by deletion of hepatic
            Cyp2c70 modulates effects of FXR activation in mice.
REFERENCE   7  (bases 1 to 1709)
  AUTHORS   Honda,A., Miyazaki,T., Iwamoto,J., Hirayama,T., Morishita,Y.,
            Monma,T., Ueda,H., Mizuno,S., Sugiyama,F., Takahashi,S. and
            Ikegami,T.
  TITLE     Regulation of bile acid metabolism in mouse models with hydrophobic
            bile acid composition
  JOURNAL   J Lipid Res 61 (1), 54-69 (2020)
   PUBMED   31645370
  REMARK    GeneRIF: Regulation of bile acid metabolism in mouse models with
            hydrophobic bile acid composition.
REFERENCE   8  (bases 1 to 1709)
  AUTHORS   Takahashi,S., Fukami,T., Masuo,Y., Brocker,C.N., Xie,C.,
            Krausz,K.W., Wolf,C.R., Henderson,C.J. and Gonzalez,F.J.
  TITLE     Cyp2c70 is responsible for the species difference in bile acid
            metabolism between mice and humans
  JOURNAL   J Lipid Res 57 (12), 2130-2137 (2016)
   PUBMED   27638959
  REMARK    GeneRIF: Cyp2c70 is a primary enzyme responsible for
            alpha-muricholic acid production.
REFERENCE   9  (bases 1 to 1709)
  AUTHORS   Scheer,N., Kapelyukh,Y., Chatham,L., Rode,A., Buechel,S. and
            Wolf,C.R.
  TITLE     Generation and characterization of novel cytochrome P450 Cyp2c gene
            cluster knockout and CYP2C9 humanized mouse lines
  JOURNAL   Mol Pharmacol 82 (6), 1022-1029 (2012)
   PUBMED   22918969
REFERENCE   10 (bases 1 to 1709)
  AUTHORS   Kasai,F., Yoshihara,M., Matsukuma,S., O'Brien,P. and
            Ferguson-Smith,M.A.
  TITLE     Emergence of complex rearrangements at translocation breakpoints in
            a transgenic mouse; implications for mechanisms involved in the
            formation of chromosome rearrangements
  JOURNAL   Cytogenet Genome Res 119 (1-2), 83-90 (2007)
   PUBMED   18160786
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK050082.1 and AC148014.3.
            
            On Nov 18, 2009 this sequence version replaced NM_145499.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC034831.1, AK050082.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1339              AK050082.1         2-1340
            1340-1709           AC148014.3         220175-220544       c
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 34.24 cM"
     gene            1..1709
                     /gene="Cyp2c70"
                     /note="cytochrome P450, family 2, subfamily c, polypeptide
                     70"
                     /db_xref="GeneID:226105"
                     /db_xref="MGI:MGI:2385878"
     exon            1..192
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1494
                     /gene="Cyp2c70"
                     /EC_number="1.14.14.1"
                     /note="cytochrome P450 2C70; CYPIIC70"
                     /codon_start=1
                     /product="cytochrome P450 2C70 precursor"
                     /protein_id="NP_663474.2"
                     /db_xref="CCDS:CCDS37976.1"
                     /db_xref="GeneID:226105"
                     /db_xref="MGI:MGI:2385878"
                     /translation="MALFIFLGIWLSCFLFLFLWNQHRGRGKLPPGPTPLPIVGNILQ
                     VDVKNISKSMGMLAKKYGPVFTVYLGMKPTVVLHGYKAMKEALIDQGDEFSDKTDSSL
                     LSRTSQGLGIVFSNGETWKQTRRFSLMVLRSMGMGKKTIEDRIQEEILYMLDALRKTN
                     GSPCDPSFLLACVPCNVISTVIFQHRFDYNDQTFQDFMENFHRKIEILASPWSQLCSA
                     YPILYYLPGIHNRFLKDVTQQKKFILEEINRHQKSLDLSNPQDFIDYFLIKMEKEKHN
                     QKSEFTMDNLVVSIGDLFGAGTETTSSTVKYGLLLLLKYPEVTAKIQEEIAHVIGRHR
                     RPTMQDRNHMPYTDAVLHEIQRYIDFVPIPSPRKTTQDVEFRGYHIPKGTSVMACLTS
                     VLNDDKEFPNPEKFDPGHFLDEKGNFKKSDYFVAFSAGRRACIGEGLARMEMFLILTN
                     ILQHFTLKPLVKPEDIDTKPVQTGLLHVPPPFELCFIPV"
     exon            193..355
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     exon            356..505
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     exon            506..666
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     exon            667..840
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     exon            841..982
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     exon            983..1170
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1312
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1709
                     /gene="Cyp2c70"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1683..1688
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp2c70"
                     /note="hexamer: AATAAA"
     polyA_site      1709
                     /gene="Cyp2c70"
                     /note="major polyA site"
ORIGIN      
        1 agtgcatttg ttcccagtga gtcaatggct ctcttcatct ttctggggat ttggctttct
       61 tgttttcttt ttctttttct atggaatcag caccgtggca gagggaagct cccacctggc
      121 cccactcctt taccaattgt tggaaatatt ttgcaagtag atgttaaaaa tattagcaaa
      181 tctatgggca tgctagcaaa aaagtatggc cctgtgttta ctgtatacct gggcatgaag
      241 cccactgtgg tgctgcatgg atacaaagca atgaaggaag ctctgattga ccagggagat
      301 gagttttctg ataaaacgga ttcatcgtta ctcagtagaa ccagccaagg cttaggcatc
      361 gtcttcagca atggagaaac atggaaacaa acccggcgtt tctccctcat ggtcttaagg
      421 tctatgggga tgggaaagaa aactattgaa gaccgaattc aggaggaaat cctttatatg
      481 ttggatgcac tgagaaaaac caatggctcc ccctgtgatc ctagcttcct tctggcctgt
      541 gttccctgca atgtgatctc tactgtcatt ttccagcatc gatttgacta caatgatcag
      601 acattccaag acttcatgga gaattttcat agaaaaatag aaattttagc atctccttgg
      661 tcccagctgt gcagtgccta ccctatttta tactatctcc ctggaatcca taacagattc
      721 ttaaaagatg tgactcaaca gaaaaagttc atcttggagg aaataaacag gcatcaaaaa
      781 tctctggacc tcagtaaccc tcaggacttt attgactact tcttgattaa aatggaaaag
      841 gaaaaacaca atcaaaaatc tgaatttact atggataacc tggttgtcag cataggtgat
      901 ctttttggag ccggaacaga gacaacgagt tccacagtga aatatgggct tttgctcctg
      961 ctgaagtacc cagaagtcac agctaaaatt caggaagaaa ttgctcatgt gattggtaga
     1021 catcggagac ccaccatgca ggacaggaac cacatgccgt acactgatgc tgtgttgcat
     1081 gagattcaga gatacattga ttttgttccc attccttcac ctcgtaagac aacgcaggat
     1141 gtggagttca gaggatacca cattcccaag ggcacaagtg taatggcatg tctgacttct
     1201 gtcctgaatg atgacaaaga atttcccaac ccagagaagt ttgatcctgg ccactttctt
     1261 gatgagaaag ggaacttcaa aaagagtgac tactttgtgg ctttctcagc aggaagaaga
     1321 gcttgtattg gagaaggtct ggcccgcatg gagatgtttc taatcctgac caacatctta
     1381 cagcatttta ctttaaaacc tctggttaaa ccagaggaca tcgacaccaa gccagttcaa
     1441 actggtttat tgcatgtgcc tccacccttt gagctctgtt tcattccagt ctgaaactgt
     1501 gtagtatttc acatggatgg ctgagaccct gcttcttctg tgagcaaaaa ggtcctcctg
     1561 tcctatgtac ctgcatctgg tagtccttta tgaatggcag ggatgctacc tctatacttt
     1621 ctcaattctt tataaaccag ttccaatttt catatgtgaa aaaacttcac tgtttcagca
     1681 ctaataaagt tttgttgcag cgtgtgtaa
//
