LOCUS       NM_001001446            1964 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus cytochrome P450, family 2, subfamily c, polypeptide 23
            (Cyp2c23), transcript variant 1, mRNA.
ACCESSION   NM_001001446 XM_129338
VERSION     NM_001001446.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ghoshal,K., Luther,J.M., Pakala,S.B., Chetyrkin,S., Falck,J.R.,
            Zent,R., Wasserman,D.H. and Pozzi,A.
  TITLE     Epoxygenase Cyp2c44 Regulates Hepatic Lipid Metabolism and Insulin
            Signaling by Controlling FATP2 Localization and Activation of the
            DAG/PKCdelta Axis
  JOURNAL   Diabetes 73 (8), 1229-1243 (2024)
   PUBMED   38743615
  REMARK    GeneRIF: Epoxygenase Cyp2c44 Regulates Hepatic Lipid Metabolism and
            Insulin Signaling by Controlling FATP2 Localization and Activation
            of the DAG/PKCdelta Axis.
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1964)
  AUTHORS   Perumal,N., Herfurth,A., Pfeiffer,N. and Manicam,C.
  TITLE     Short-Term Omega-3 Supplementation Modulates Novel Neurovascular
            and Fatty Acid Metabolic Proteome Changes in the Retina and
            Ophthalmic Artery of Mice with Targeted Cyp2c44 Gene Deletion
  JOURNAL   Cells 11 (21), 3494 (2022)
   PUBMED   36359890
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1964)
  AUTHORS   Ghoshal,K., Li,X., Peng,D., Falck,J.R., Anugu,R.R., Chiusa,M.,
            Stafford,J.M., Wasserman,D.H., Zent,R., Luther,J.M. and Pozzi,A.
  TITLE     EET Analog Treatment Improves Insulin Signaling in a Genetic Mouse
            Model of Insulin Resistance
  JOURNAL   Diabetes (2021) In press
   PUBMED   34957494
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   5  (bases 1 to 1964)
  AUTHORS   Hu,J., Sisignano,M., Brecht,R., Perumal,N., Angioni,C., Bibli,I.S.,
            Fisslthaler,B., Kleinert,H., Pfeiffer,N., Fleming,I. and Manicam,C.
  TITLE     Cyp2c44 epoxygenase-derived epoxyeicosatrienoic acids in vascular
            smooth muscle cells elicit vasoconstriction of the murine
            ophthalmic artery
  JOURNAL   Sci Rep 11 (1), 18764 (2021)
   PUBMED   34548575
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1964)
  AUTHORS   Pidkovka,N., Rao,R., Mei,S., Gong,Y., Harris,R.C., Wang,W.H. and
            Capdevila,J.H.
  TITLE     Epoxyeicosatrienoic acids (EETs) regulate epithelial sodium channel
            activity by extracellular signal-regulated kinase 1/2
            (ERK1/2)-mediated phosphorylation
  JOURNAL   J Biol Chem 288 (7), 5223-5231 (2013)
   PUBMED   23283969
  REMARK    GeneRIF: characterization of a role for the murine CYP2C44
            epoxygenase and 14,15-EET in the ERK1/2-mediated regulation of
            amiloride-sensitive JNa+
REFERENCE   7  (bases 1 to 1964)
  AUTHORS   Sun,P., Antoun,J., Lin,D.H., Yue,P., Gotlinger,K.H., Capdevila,J.
            and Wang,W.H.
  TITLE     Cyp2c44 epoxygenase is essential for preventing the renal sodium
            absorption during increasing dietary potassium intake
  JOURNAL   Hypertension 59 (2), 339-347 (2012)
   PUBMED   22184322
  REMARK    GeneRIF: Cyp2c44 functions as an high potassium-inducible
            antihypertensive enzyme responsible for inhibiting ENaC activity
            and Na absorption in the aldosterone-sensitive distal nephron.
REFERENCE   8  (bases 1 to 1964)
  AUTHORS   Pozzi,A., Popescu,V., Yang,S., Mei,S., Shi,M., Puolitaival,S.M.,
            Caprioli,R.M. and Capdevila,J.H.
  TITLE     The anti-tumorigenic properties of peroxisomal
            proliferator-activated receptor alpha are arachidonic acid
            epoxygenase-mediated
  JOURNAL   J Biol Chem 285 (17), 12840-12850 (2010)
   PUBMED   20178979
  REMARK    GeneRIF: The anti-tumorigenic properties of peroxisomal
            proliferator-activated receptor alpha are arachidonic acid
            epoxygenase-mediated.
REFERENCE   9  (bases 1 to 1964)
  AUTHORS   Yang,S., Wei,S., Pozzi,A. and Capdevila,J.H.
  TITLE     The arachidonic acid epoxygenase is a component of the signaling
            mechanisms responsible for VEGF-stimulated angiogenesis
  JOURNAL   Arch Biochem Biophys 489 (1-2), 82-91 (2009)
   PUBMED   19464254
  REMARK    GeneRIF: Cyp2c44 arachidonic acid epoxygenase is a component of the
            signaling pathways associated with VEGF-stimulated angiogenesis
REFERENCE   10 (bases 1 to 1964)
  AUTHORS   DeLozier,T.C., Tsao,C.C., Coulter,S.J., Foley,J., Bradbury,J.A.,
            Zeldin,D.C. and Goldstein,J.A.
  TITLE     CYP2C44, a new murine CYP2C that metabolizes arachidonic acid to
            unique stereospecific products
  JOURNAL   J Pharmacol Exp Ther 310 (3), 845-854 (2004)
   PUBMED   15084647
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124693.4 and AK149451.1.
            
            On Nov 19, 2009 this sequence version replaced NM_001001446.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC026766.1, BK005218.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                AC124693.4         92578-92616         c
            40-1432             AK149451.1         2-1394
            1433-1964           AC124693.4         68391-68922         c
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 36.69 cM"
     gene            1..1964
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /note="cytochrome P450, family 2, subfamily c, polypeptide
                     23"
                     /db_xref="GeneID:226143"
                     /db_xref="MGI:MGI:1888897"
     exon            1..240
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1545
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /EC_number="1.14.14.1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; cytochrome P450, 2c44; Mutant line 386;
                     cytochrome P450, family 2, subfamily c, polypeptide 44;
                     arachidonic acid epoxygenase"
                     /codon_start=1
                     /product="cytochrome P450 2C44 isoform 1 precursor"
                     /protein_id="NP_001001446.3"
                     /db_xref="CCDS:CCDS29841.1"
                     /db_xref="GeneID:226143"
                     /db_xref="MGI:MGI:1888897"
                     /translation="MELLGLPTLALLVLVMSLSLLSVWTKMRTGGRLPPGPTPLPIIG
                     NILQLDLKDIPASLSKLAKEYGPVYTLYFGSWPTVVLHGYDVVKEALLNQGDEFLGRG
                     PLPIIEDSQKGHGIVFSEGERWKLLRRFSLMTLKNFGMGKRSLEERVQEEARCLVEEL
                     HKTEAQPFDPTFILACAPCNVICSILFNERFPYNDKTFLNLMDLLNKNFYQLNSIWIQ
                     MYNLWPTIMKYIPGKHREFSKRLGGVKNFILEKVKEHQESLDPANPRDYIDCFLSKIE
                     EEKHNLKSDFNLENLAICGSNLFTAGTETTSTTLRFGLLLLVKHPEVQAKVHEELDRV
                     IGRHQPPSMKDKMKLPYTDAVLHEIQRYITLLPSSLPHAVVQDTKFRHYVIPKGTAVF
                     PFLSSILLDQKEFPNPEKFDPGHFLDKNGCFKKTDYFVPFSLGKRSCVGEGLARMELF
                     LFFTTILQKFSLKALVEPKDLDIKPVTTGLFNLPPPYKLRLVPR"
     exon            241..403
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     exon            404..553
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     exon            554..714
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     exon            715..891
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1033
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1221
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1363
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1964
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1942..1947
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /note="hexamer: AATAAA"
     polyA_site      1964
                     /gene="Cyp2c23"
                     /gene_synonym="b2b386Clo; Cyp2c44"
                     /note="major polyA site"
ORIGIN      
        1 attattgggg cttaagcttt gctcacaaca ggcagcttca gttaacatcc cggggctgac
       61 atggagctgc tgggtctccc tacgcttgct ctcctggttt tggtgatgtc cctcagcctc
      121 ctttcagtgt ggacaaaaat gcgcactggg gggagactgc cccctggccc cactccactt
      181 cccattattg ggaatatact gcaattggac ctcaaagaca tccctgcatc gctttccaag
      241 ctggccaaag agtatgggcc tgtttacact ctgtactttg gatcctggcc taccgtggtc
      301 ctacatggct atgatgtggt gaaggaagcg ctactcaatc aaggtgatga gttccttggg
      361 agaggacctc tgcccattat cgaagatagc cagaagggac atggaattgt tttcagtgaa
      421 ggagagaggt ggaaactcct gcgacgcttc tccctcatga cgctgaagaa cttcggaatg
      481 gggaagagaa gcctagagga gagggtgcag gaggaagccc ggtgcctcgt ggaggagtta
      541 cataaaacag aagctcagcc ctttgacccc accttcatcc tggcctgtgc tccctgcaac
      601 gtgatctgct ccatcctttt caacgagcga ttcccataca atgacaagac attcctcaac
      661 ctcatggacc ttttaaataa aaatttttac caactgaact ctatatggat ccagatgtac
      721 aacctatggc cgacgatcat gaagtacata cctggaaagc atagagaatt ctcaaaaagg
      781 cttggtggtg ttaaaaactt catcctagaa aaagtgaagg agcaccagga gtccctggac
      841 cccgccaacc ctcgggatta catcgactgt ttcctcagca agatcgagga ggagaaacac
      901 aacctgaaat ctgattttaa tctggagaat ttggccatct gtgggtctaa cttgtttacg
      961 gcaggcacag agacaaccag caccaccctg agattcgggc ttctgctcct tgtcaagcat
     1021 ccagaggtgc aagccaaagt tcatgaagaa cttgatcgtg tgattggacg ccaccaaccc
     1081 cccagcatga aggacaagat gaagctgccc tatacagatg ccgtgctgca tgagattcaa
     1141 agatacatca ctctccttcc ttctagtctg ccccatgctg tggtccagga cacaaaattc
     1201 agacactatg tcatccccaa gggcaccgct gtgttcccgt ttctgtcttc catcttgttg
     1261 gatcaaaagg agtttcccaa cccagagaag tttgatccag gacactttct ggataaaaat
     1321 ggctgcttca agaaaacaga ctactttgtt cctttctccc tcgggaagcg atcctgtgtt
     1381 ggcgagggtt tggcccgcat ggagctcttc ctgttcttca ccactatcct gcaaaaattt
     1441 tccctaaagg ctctggtgga gccaaaggac cttgatatca agcctgttac tactgggctt
     1501 ttcaacttgc caccgcctta caagctgcgc cttgttccaa gataggagtt tctcctttta
     1561 atcattcaaa tcaaagtaat tgttctcttt ttttctataa acagtctatt ttgctaagtg
     1621 ctacctaggc cagaaacatc actcactaac agcacgtggg catttgtttc catcttaaaa
     1681 cctgggtcct gaaagctccc aggacacagg cagggtgtct cagttcctca gctgtctata
     1741 aactaggccc taagcacaac tgacaccaga tccacaaggc cctcatcaga cgccacctgc
     1801 aagcagctgc gtgctcttct gtggcttcag aaatagaatt atattctgtc actgtttcaa
     1861 tagagaaatt aatgtaccag cccacacaca ttttcctatt ctcacgtaaa agcatttatg
     1921 tggtttgtgt caccttcatg aaataaattc tcaccccaat aata
//
