LOCUS       NM_009997               1715 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus cytochrome P450, family 2, subfamily a, polypeptide 4
            (Cyp2a4), mRNA.
ACCESSION   NM_009997 XM_907266
VERSION     NM_009997.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Oguro,A., Fujiyama,T., Ishihara,Y., Kataoka,C., Yamamoto,M.,
            Eto,K., Komohara,Y., Imaoka,S., Sakuragi,T., Tsuji,M., Shibata,E.,
            Kotake,Y. and Yamazaki,T.
  TITLE     Maternal DHA intake in mice increased DHA metabolites in the pup
            brain and ameliorated MeHg-induced behavioral disorder
  JOURNAL   J Lipid Res 64 (11), 100458 (2023)
   PUBMED   37838304
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Nail,A.N., Spear,B.T. and Peterson,M.L.
  TITLE     Highly homologous mouse Cyp2a4 and Cyp2a5 genes are differentially
            expressed in the liver and both express long non-coding antisense
            RNAs
  JOURNAL   Gene 767, 145162 (2021)
   PUBMED   32987105
REFERENCE   3  (bases 1 to 1715)
  AUTHORS   Chikada,H., Ida,K., Ando,E., Inagaki,Y., Sakamoto,A. and Kamiya,A.
  TITLE     Establishment and analysis of a mouse model that regulates
            sex-related differences in liver drug metabolism
  JOURNAL   Lab Invest 98 (11), 1500-1511 (2018)
   PUBMED   29968852
REFERENCE   4  (bases 1 to 1715)
  AUTHORS   Creasy,K.T., Jiang,J., Ren,H., Peterson,M.L. and Spear,B.T.
  TITLE     Zinc Fingers and Homeoboxes 2 (Zhx2) Regulates Sexually Dimorphic
            Cyp Gene Expression in the Adult Mouse Liver
  JOURNAL   Gene Expr 17 (1), 7-17 (2016)
   PUBMED   27197076
REFERENCE   5  (bases 1 to 1715)
  AUTHORS   Li,L., Jia,K., Zhou,X., McCallum,S.E., Hough,L.B. and Ding,X.
  TITLE     Impact of nicotine metabolism on nicotine's pharmacological effects
            and behavioral responses: insights from a Cyp2a(4/5)bgs-null mouse
  JOURNAL   J Pharmacol Exp Ther 347 (3), 746-754 (2013)
   PUBMED   24045421
  REMARK    GeneRIF: we found that the Cyp2a(4/5)bgs-null mice developed a
            preference for nicotine in a conditioned place preference test
REFERENCE   6  (bases 1 to 1715)
  AUTHORS   Salonpaa,P., Iscan,M., Pasanen,M., Arvela,P., Pelkonen,O. and
            Raunio,H.
  TITLE     Cerium-induced strain-dependent increase in Cyp2a-4/5 (cytochrome
            P4502a-4/5) expression in the liver and kidneys of inbred mice
  JOURNAL   Biochem Pharmacol 44 (7), 1269-1274 (1992)
   PUBMED   1417950
REFERENCE   7  (bases 1 to 1715)
  AUTHORS   Burkhart,B.A., Skow,L.C. and Negishi,M.
  TITLE     Two steroid 15 alpha-hydroxylase genes and a homologous gene family
            in mice
  JOURNAL   Gene 87 (2), 205-211 (1990)
   PUBMED   1970547
REFERENCE   8  (bases 1 to 1715)
  AUTHORS   Lindberg,R.L. and Negishi,M.
  TITLE     Alteration of mouse cytochrome P450coh substrate specificity by
            mutation of a single amino-acid residue
  JOURNAL   Nature 339 (6226), 632-634 (1989)
   PUBMED   2733794
REFERENCE   9  (bases 1 to 1715)
  AUTHORS   Lindberg,R., Burkhart,B., Ichikawa,T. and Negishi,M.
  TITLE     The structure and characterization of type I P-450(15) alpha gene
            as major steroid 15 alpha-hydroxylase and its comparison with type
            II P-450(15) alpha gene
  JOURNAL   J Biol Chem 264 (11), 6465-6471 (1989)
   PUBMED   2703500
REFERENCE   10 (bases 1 to 1715)
  AUTHORS   Burkhart,B.A., Harada,N. and Negishi,M.
  TITLE     Sexual dimorphism of testosterone 15 alpha-hydroxylase mRNA levels
            in mouse liver. cDNA cloning and regulation
  JOURNAL   J Biol Chem 260 (28), 15357-15361 (1985)
   PUBMED   2415518
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC063778.1.
            
            On Oct 19, 2006 this sequence version replaced NM_009997.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063778.1, SRR5189682.114120.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1715              BC063778.1         24-1738
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="FVB/N"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 14.39 cM"
     gene            1..1715
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /note="cytochrome P450, family 2, subfamily a, polypeptide
                     4"
                     /db_xref="GeneID:13086"
                     /db_xref="MGI:MGI:88596"
     exon            1..206
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     CDS             27..1511
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /EC_number="1.14.14.1"
                     /note="CYPIIA4; cytochrome P450-IIA3.1; cytochrome
                     P450-15-alpha; testosterone 15-alpha-hydroxylase;
                     cytochrome P450, 2a4; testosterone 15alpha-hydroxylase"
                     /codon_start=1
                     /product="cytochrome P450 2A4"
                     /protein_id="NP_034127.2"
                     /db_xref="CCDS:CCDS21002.1"
                     /db_xref="GeneID:13086"
                     /db_xref="MGI:MGI:88596"
                     /translation="MLTSGLLLVAAVAFLSVLVLMSVWKQRKLSGKLPPGPTPLPFVG
                     NFLQLNTEQMYNSLMKISQRYGPVFTIYLGSRRIVVLCGQETVKEALVDQAEEFSGRG
                     EQATFDWLFKGYGIAFSSGERAKQLRRFSITTLRDFGVGKRGIEERIQEEAGFLIDSF
                     RKTNGAFIDPTFYLSRTVSNVISSIVFGDRFDYEDKEFLSLLRMMLGSLQFTATSMGQ
                     VYEMFSSVMKHLPGPQQQAFKELQGLEDFITKKVEHNQRTLDPNSPRDFIDSFLIRML
                     EEKKNPNTEFYMKNLVLTTLNLFFAGTETVSTTLRYGFLLLMKYPDIEAKVHEEIDRV
                     IGRNRQPKYEDRMKMPYTEAVIHEIQRFADLIPMGLARRVTKDTKFRDFLLPKGTEVF
                     PMLGSVLKDPKFFSNPKDFNPKHFLDDKGQFKKSDAFVPFSIGKRYCFGEGLARMELF
                     LFLTNIMQNFHFKSTQEPQDIDVSPRLVGFVTIPPTYTMSFLSR"
     exon            207..369
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     exon            370..519
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     exon            520..680
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     exon            681..857
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     exon            858..999
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1187
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     exon            1188..1329
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1715
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1690..1695
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /note="hexamer: AATAAA"
     polyA_site      1715
                     /gene="Cyp2a4"
                     /gene_synonym="Cyp15a1; D7Ucla4"
                     /note="major polyA site"
ORIGIN      
        1 tgtctgtcta tcaccccact gccaccatgc tgacctcagg actcctcctg gtggctgcag
       61 tggccttcct cagcgtcctg gttttgatgt ctgtctggaa gcagagaaag ctctcaggaa
      121 agctgcctcc aggacccacc ccactgccct tcgttgggaa cttccttcag ctgaacacag
      181 agcaaatgta caactctctc atgaagatca gccaacgtta tggtcctgta ttcaccatct
      241 atctgggatc tcgccgaatt gtggtgctgt gcggacagga gacagtcaag gaagctctgg
      301 tggaccaagc tgaggaattc agcgggcggg gcgagcaggc taccttcgac tggcttttca
      361 aaggctatgg catagccttc agcagcgggg agcgagccaa acagctaagg cgcttctcca
      421 tcaccacgct gcgggacttc ggcgtgggga agcgtggcat cgaggagcgc atccaagagg
      481 aggcgggctt tctcatcgat tcatttcgga agacgaacgg tgctttcatt gaccccacct
      541 tctaccttag ccgaacagtc tccaatgtca ttagctcaat cgtcttcggg gaccgctttg
      601 actatgagga caaagagttc ctgtcacttc ttagaatgat gctgggaagc ctccagttca
      661 ctgctacctc catggggcag gtctatgaga tgttttcttc tgtgatgaaa cacctgccag
      721 ggccccagca acaggccttt aaggagctgc aaggcctgga ggactttata accaagaaag
      781 tggaacacaa tcagcgcacg ctggatccca attccccaag ggacttcatc gactccttcc
      841 tcatccgaat gctggaggag aagaagaacc ccaatactga gttctacatg aagaacttgg
      901 tgctgactac actaaatctc ttctttgctg gcacagagac cgtcagcacc accctgcgct
      961 atggctttct gttgctcatg aagtacccag atattgaggc caaggtccat gaggagattg
     1021 atcgggtgat tggcaggaac cggcagccca agtatgagga ccgaatgaag atgccctata
     1081 cggaggctgt aatccatgag atccagagat ttgcagacct gatccccatg ggcctggctc
     1141 gaagggtcac caaggacacc aagtttcgag atttcctcct ccccaagggt actgaagtat
     1201 ttcctatgct gggctctgtg ctgaaagacc ccaagttctt ctccaacccc aaagacttca
     1261 acccaaagca cttcctagat gacaagggac agtttaagaa gagtgatgcc tttgtgccct
     1321 tttccattgg aaaacggtat tgtttcggag aaggactggc taggatggaa ctcttcctct
     1381 tcctcacaaa catcatgcag aacttccact tcaaatccac acaggaaccc caggacatcg
     1441 atgtgtctcc tagactcgtg ggctttgtca cgatcccacc aacctacact atgagtttct
     1501 tgtcccgttg atcctgggct gcatgaggtt aaagggaatg attgagacca gacaagtcag
     1561 gggttgaaac ttagaaaagg tcaaaggtac agaagaaaca gaggacactt cgtagacttg
     1621 cagaggatat ttcaaaggta gccagagaag ggggaaatta tactatgttg tcaataggaa
     1681 taataaaata ataaaagtag atattattta tggca
//
