LOCUS       NM_133657               1675 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus cytochrome P450, family 2, subfamily a, polypeptide 12
            (Cyp2a12), mRNA.
ACCESSION   NM_133657
VERSION     NM_133657.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Honda,A., Miyazaki,T., Iwamoto,J., Hirayama,T., Morishita,Y.,
            Monma,T., Ueda,H., Mizuno,S., Sugiyama,F., Takahashi,S. and
            Ikegami,T.
  TITLE     Regulation of bile acid metabolism in mouse models with hydrophobic
            bile acid composition
  JOURNAL   J Lipid Res 61 (1), 54-69 (2020)
   PUBMED   31645370
  REMARK    GeneRIF: Regulation of bile acid metabolism in mouse models with
            hydrophobic bile acid composition.
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Lee,J., Xu,Y., Chen,Y., Sprung,R., Kim,S.C., Xie,S. and Zhao,Y.
  TITLE     Mitochondrial phosphoproteome revealed by an improved IMAC method
            and MS/MS/MS
  JOURNAL   Mol Cell Proteomics 6 (4), 669-676 (2007)
   PUBMED   17208939
REFERENCE   3  (bases 1 to 1675)
  AUTHORS   Wang,H., Donley,K.M., Keeney,D.S. and Hoffman,S.M.
  TITLE     Organization and evolution of the Cyp2 gene cluster on mouse
            chromosome 7, and comparison with the syntenic human cluster
  JOURNAL   Environ Health Perspect 111 (15), 1835-1842 (2003)
   PUBMED   14630516
REFERENCE   4  (bases 1 to 1675)
  AUTHORS   Choudhary,D., Jansson,I., Schenkman,J.B., Sarfarazi,M. and
            Stoilov,I.
  TITLE     Comparative expression profiling of 40 mouse cytochrome P450 genes
            in embryonic and adult tissues
  JOURNAL   Arch Biochem Biophys 414 (1), 91-100 (2003)
   PUBMED   12745259
REFERENCE   5  (bases 1 to 1675)
  AUTHORS   Iwasaki,M., Lindberg,R.L., Juvonen,R.O. and Negishi,M.
  TITLE     Site-directed mutagenesis of mouse steroid 7 alpha-hydroxylase
            (cytochrome P-450(7) alpha): role of residue-209 in determining
            steroid-cytochrome P-450 interaction
  JOURNAL   Biochem J 291 (Pt 2) (Pt 2), 569-573 (1993)
   PUBMED   8484736
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC018356.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC018356.1, BC094017.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="FVB/N"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 15.77 cM"
     gene            1..1675
                     /gene="Cyp2a12"
                     /note="cytochrome P450, family 2, subfamily a, polypeptide
                     12"
                     /db_xref="GeneID:13085"
                     /db_xref="MGI:MGI:105055"
     exon            1..193
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     CDS             17..1495
                     /gene="Cyp2a12"
                     /EC_number="1.14.14.1"
                     /note="CYPIIA12; testosterone 7-alpha-hydroxylase; steroid
                     hormones 7-alpha-hydroxylase; cytochrome P450, 2a12"
                     /codon_start=1
                     /product="cytochrome P450 2A12"
                     /protein_id="NP_598418.1"
                     /db_xref="CCDS:CCDS21009.1"
                     /db_xref="GeneID:13085"
                     /db_xref="MGI:MGI:105055"
                     /translation="MLGSGLLLLAILAFLSVMVLVSVWQQKIRGKLPPGPIPLPFIGN
                     YLQLNRKDVYSSITQLQEHYGPVFTIHLGPRRVVVLYGYDAVKEALVDHAEEFSGRGE
                     QATFNTLFKGYGVAFSNGERAKQLRRFSIATLRDFGMGKRGVEERIQEEAGCLIKMLQ
                     GTCGAPIDPTIYLSKTASNVISSIVFGDRFNYEDKEFLSLLQMMGQVNKFAASPTGQL
                     YDMFHSVMKYLPGPQQQIIKDSHKLEDFMIQKVKQNQSTLDPNSPRDFIDSFLIHMQK
                     EKYVNSEFHMKNLVMTSLNLFFAGSETVSSTLRYGFLLLMKHPDVEAKVHEEIDRVIG
                     RNRQPQYEDHMKMPYTQAVINEIQRFSNFAPLGIPRRITKDTSFRGFFLPKGTEVFPI
                     LGSLMTDPKFFSSPKDFNPQHFLDDKGQLKKIPAFLPFSTGKRFCLGDSLAKMELFLF
                     FTTILQNFRFKFPRKLEDINESPTPEGFTRIIPKYTMSFVPI"
     exon            194..356
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     exon            357..506
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     exon            507..667
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     exon            668..844
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     exon            845..983
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1171
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1313
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1667
                     /gene="Cyp2a12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1640..1645
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp2a12"
                     /note="hexamer: GATAAA"
     polyA_site      1667
                     /gene="Cyp2a12"
                     /note="major polyA site"
ORIGIN      
        1 atcctcattg gccaccatgc tgggctcagg gctgctcctc ctagccattc tggccttcct
       61 gagtgtgatg gtcttggtgt ctgtctggca gcagaaaatc agggggaagt tgcccccagg
      121 acccattcct ttgcctttca ttggaaatta cctgcaactg aatagaaaag atgtatacag
      181 ctccatcaca cagctccaag agcactatgg ccctgtgttc accatccacc ttgggcctcg
      241 ccgggttgtg gtgctttatg gatacgatgc agtcaaagag gctttggtgg accacgctga
      301 ggagttcagt ggacgaggcg aacaggccac ctttaataca ctcttcaaag gctatggtgt
      361 ggcattcagc aatggggaac gagccaaaca actcagacgc ttctctatag ccacactgag
      421 agatttcggc atgggcaagc gaggtgtgga ggagcgcatc caggaggagg caggctgttt
      481 gatcaagatg ttgcagggca cttgtggagc ccccattgac cccaccatct acctgagcaa
      541 aacagcctcc aatgtcatta gctccattgt cttcggcgac cgcttcaact atgaggacaa
      601 agagttcctg tcactgctgc agatgatggg tcaagtgaac aaatttgctg cttcacccac
      661 agggcagctc tatgacatgt tccattcagt tatgaagtac ctgcccggac cacagcaaca
      721 gatcatcaag gattctcata aactggaaga cttcatgata cagaaagtga agcagaacca
      781 gagtaccctg gaccccaatt ccccacgaga cttcattgac tcctttctca tccacatgca
      841 aaaggagaaa tatgttaatt cagagtttca catgaagaac ctagtgatga catcattgaa
      901 cctcttcttt gctgggtctg agacagtcag ctccacacta cgatatggct tcctgctact
      961 aatgaagcat ccagatgtag aggccaaggt ccatgaggag attgaccgag tgattggcag
     1021 gaaccgacag ccccaatatg aggaccatat gaagatgccc tacacccagg ctgtgatcaa
     1081 tgagatccag agattttcta actttgctcc cttgggcatt cctcgaagga ttaccaagga
     1141 tacttccttc cgtggcttct tcctccccaa gggcactgaa gtgtttccta tactgggttc
     1201 tctgatgaca gacccaaagt tcttctctag ccccaaagac ttcaacccac agcacttcct
     1261 ggatgacaag ggacagttga agaagatccc tgcttttctg cccttttcca ctgggaagcg
     1321 attctgcttg ggagacagcc tggctaagat ggagctgttc ctgttcttca ccaccatctt
     1381 gcagaacttc cgtttcaagt tcccaaggaa actagaagat atcaatgagt cccctacacc
     1441 agaggggttt accaggatca taccaaagta caccatgagc ttcgtgccca tctgattctg
     1501 ggttgagcca agatgggtca agagggaggg agagcctgaa gtggtgctag aaggaaggtg
     1561 gagtgaagag agaagataaa gacctgggga ttaagaaggg accacgtgca tggaagaaat
     1621 agaaaacact actcaccttg ataaaattgt aacagtaata ataaaaaaaa aaaaa
//
