LOCUS       NM_024264               1936 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cytochrome P450, family 27, subfamily a, polypeptide 1
            (Cyp27a1), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_024264
VERSION     NM_024264.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Ito,T., Ichikawa,T., Yamada,M., Hashimoto,Y., Fujino,N.,
            Numakura,T., Sasaki,Y., Suzuki,A., Takita,K., Sano,H., Kyogoku,Y.,
            Saito,T., Koarai,A., Tamada,T. and Sugiura,H.
  TITLE     CYP27A1-27-hydroxycholesterol axis in the respiratory system
            contributes to house dust mite-induced allergic airway inflammation
  JOURNAL   Allergol Int 73 (1), 151-163 (2024)
   PUBMED   37607853
  REMARK    GeneRIF: CYP27A1-27-hydroxycholesterol axis in the respiratory
            system contributes to house dust mite-induced allergic airway
            inflammation.
REFERENCE   3  (bases 1 to 1936)
  AUTHORS   Goikolea,J., Latorre-Leal,M., Tsagkogianni,C., Pikkupeura,S.,
            Gulyas,B., Cedazo-Minguez,A., Loera-Valencia,R., Bjorkhem,I.,
            Rodriguez Rodriguez,P. and Maioli,S.
  TITLE     Different effects of CYP27A1 and CYP7B1 on cognitive function: Two
            mouse models in comparison
  JOURNAL   J Steroid Biochem Mol Biol 234, 106387 (2023)
   PUBMED   37648096
  REMARK    GeneRIF: Different effects of CYP27A1 and CYP7B1 on cognitive
            function: Two mouse models in comparison.
REFERENCE   4  (bases 1 to 1936)
  AUTHORS   Fang,Z., Cheng,G., He,M. and Lin,Y.
  TITLE     CYP27A1 deficiency promoted osteoclast differentiation
  JOURNAL   PeerJ 11, e15041 (2023)
   PUBMED   36890868
  REMARK    GeneRIF: CYP27A1 deficiency promoted osteoclast differentiation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1936)
  AUTHORS   Ceglia,S., Berthelette,A., Howley,K., Li,Y., Mortzfeld,B.,
            Bhattarai,S.K., Yiew,N.K.H., Xu,Y., Brink,R., Cyster,J.G.,
            Hooper,L.V., Randolph,G.J., Bucci,V. and Reboldi,A.
  TITLE     An epithelial cell-derived metabolite tunes immunoglobulin A
            secretion by gut-resident plasma cells
  JOURNAL   Nat Immunol 24 (3), 531-544 (2023)
   PUBMED   36658240
REFERENCE   6  (bases 1 to 1936)
  AUTHORS   Rosen,H., Reshef,A., Maeda,N., Lippoldt,A., Shpizen,S., Triger,L.,
            Eggertsen,G., Bjorkhem,I. and Leitersdorf,E.
  TITLE     Markedly reduced bile acid synthesis but maintained levels of
            cholesterol and vitamin D metabolites in mice with disrupted sterol
            27-hydroxylase gene
  JOURNAL   J Biol Chem 273 (24), 14805-14812 (1998)
   PUBMED   9614081
REFERENCE   7  (bases 1 to 1936)
  AUTHORS   Stromstedt,M., Keeney,D.S., Waterman,M.R., Paria,B.C., Conley,A.J.
            and Dey,S.K.
  TITLE     Preimplantation mouse blastocysts fail to express CYP genes
            required for estrogen biosynthesis
  JOURNAL   Mol Reprod Dev 43 (4), 428-436 (1996)
   PUBMED   9052933
REFERENCE   8  (bases 1 to 1936)
  AUTHORS   Spur,N.K., Bashir,R., Bushby,K., Cox,A., Cox,S., Hilde Brandt,F.,
            Hill,N., Kao,F.T., Krols,L., Marzella,R., Miller,N., Nothwang,H.G.,
            Rocchi,M., Sarfarazi,M., Stratakis,C.A., Wallgren-Petterson,C. and
            Naylor,S.
  TITLE     Report and abstracts of the Fourth International Workshop on Human
            Chromosome 2 Mapping 1996
  JOURNAL   Cytogenet Cell Genet 73 (4), 255-273 (1996)
   PUBMED   8751373
REFERENCE   9  (bases 1 to 1936)
  AUTHORS   Cali,J.J., Hsieh,C.L., Francke,U. and Russell,D.W.
  TITLE     Mutations in the bile acid biosynthetic enzyme sterol
            27-hydroxylase underlie cerebrotendinous xanthomatosis
  JOURNAL   J Biol Chem 266 (12), 7779-7783 (1991)
   PUBMED   2019602
REFERENCE   10 (bases 1 to 1936)
  AUTHORS   Cali,J.J. and Russell,D.W.
  TITLE     Characterization of human sterol 27-hydroxylase. A mitochondrial
            cytochrome P-450 that catalyzes multiple oxidation reaction in bile
            acid biosynthesis
  JOURNAL   J Biol Chem 266 (12), 7774-7778 (1991)
   PUBMED   1708392
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CV560359.1, AK004977.1 and AC152409.8.
            
            On Sep 1, 2015 this sequence version replaced NM_024264.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC055028.1, AK004977.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                CV560359.1         1-39
            40-1929             AK004977.1         2-1891
            1930-1936           AC152409.8         71990-71996         c
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 38.54 cM"
     gene            1..1936
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /note="cytochrome P450, family 27, subfamily a,
                     polypeptide 1"
                     /db_xref="GeneID:104086"
                     /db_xref="MGI:MGI:88594"
     exon            1..357
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..1698
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /EC_number="1.14.15.15"
                     /note="cytochrome P450, 27a1; sterol 26-hydroxylase,
                     mitochondrial; sterol 27-hydroxylase; cytochrome
                     P-450C27/25; vitamin D(3) 25-hydroxylase;
                     5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol
                     27-hydroxylase; cholesterol 27 hydroxylase;
                     5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol
                     26-hydroxylase"
                     /codon_start=1
                     /product="sterol 26-hydroxylase, mitochondrial precursor"
                     /protein_id="NP_077226.2"
                     /db_xref="CCDS:CCDS15054.1"
                     /db_xref="GeneID:104086"
                     /db_xref="MGI:MGI:88594"
                     /translation="MAAWSRTRLRWTLLDPRVVGRGLCPQGARAKATIPAALQAQEST
                     EGPGTGQDRPRLRSPAELPGTGTLQFLFQLFLQGYVLHLPDLQVLNKTKYGPMWTTSF
                     GTYTNVNLASAPLLEQVMRQEGKYPIRDHMDQWKDHRDHKGLTYGIFIAQGEQWYHLR
                     QALKQRLLKPDEAALYTDALNEVISDFITRLDQVRAESESGDQVPDMAHLLYHLALEA
                     ITYILFEKRIGCLKPSIPEDTAAFIRSVAIMFQNSVYITFLPKWTRPLLPFWKRYLNG
                     WDNIFSFGKKLIDEKVQELKAQLQETGPDGVRVSGYLHFLLTNELLSTQETIGTFPEL
                     LLAGVDTTSNTLTWALYHLSKSPEIQEALHKEVTGVVPFGKVPQHKDFAHMPLLKAVI
                     KETLRLYPVVPTNSRIITEKETEINGFLFPKNTQFVLCHYVVSRDPSVFPEPNSFQPH
                     RWLRKKEADNPGILHPFGSVPFGYGVRSCLGRRIAELEMQLMLSRLVQKYEIALAPGM
                     GEVKTVSRIVLVPSKKVRLHFLQRQ"
     exon            358..548
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     exon            549..748
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     exon            749..946
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1119
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     exon            1120..1286
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1368
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1581
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     exon            1582..1936
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1913..1918
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /note="hexamer: AATAAA"
     polyA_site      1931
                     /gene="Cyp27a1"
                     /gene_synonym="1300013A03Rik; Cyp27"
                     /note="major polyA site"
ORIGIN      
        1 gtcgccaggt cgcaaagccc gagttctgtc tgcctccttg cctggatagg gctcatagtc
       61 tctggatcta aactcttggc ttctcgggca cgatccatgg ctgcgtggag ccgcacgagg
      121 ctgagatgga cgctcctgga cccgcgtgtg gtgggccgtg gcctctgccc acaaggggcc
      181 agagccaagg ccacgatccc tgcagccctc caggcacagg agagtacgga gggtccagga
      241 acaggtcaag accggccgcg cctgcggagt ccggcggagc ttccggggac cggaacgcta
      301 caatttttat tccagctatt tctacaaggc tatgtgctgc acttgcccga cctccaggtg
      361 ctgaacaaga ccaagtatgg tccaatgtgg acaacctcct ttgggactta caccaatgtg
      421 aatctggcta gtgccccact cttggagcaa gtgatgagac aggagggcaa gtacccaata
      481 agagaccaca tggatcagtg gaaggaccac cgagaccaca agggcctcac ctatgggatc
      541 ttcatcgcac aaggagagca atggtaccat ctgcgtcagg ctttgaaaca gaggctgctg
      601 aagcctgacg aggccgcgct ctacacggat gccttaaacg aggttatcag tgactttatc
      661 acccggctgg accaggtgcg ggcagagagt gaatcagggg accaggtgcc agacatggct
      721 catcttctct accaccttgc cttggaagcc atcacctata tcctgtttga gaaaaggatt
      781 ggctgcctga aaccctccat tcctgaggac actgccgcct tcatcagatc tgttgcaatc
      841 atgttccaga actcagtcta tatcactttc cttcccaaat ggacgcgtcc tctgctgccc
      901 ttttggaagc gatacctgaa tggctgggat aacattttct cctttggaaa gaagctgatt
      961 gatgaaaaag tccaggagct aaaagcccag ctacaggaaa ctgggccaga tggagtccgg
     1021 gtatctggct acctgcactt cctgctgacc aatgaattgc tcagtactca ggagaccatc
     1081 ggcacctttc ctgagctgct tttggctggg gtggacacga catccaacac actgacatgg
     1141 gccctgtacc acctttcaaa gagcccagag atccaggagg ccttgcacaa ggaagtgact
     1201 ggtgtggtgc ccttcgggaa ggtgccccag cacaaggact ttgcccacat gcctctgcta
     1261 aaagctgtga ttaaggagac cctgcgcctc taccctgtgg ttcccacaaa ctcccggatc
     1321 atcacagaaa aggaaactga aattaatggc tttctcttcc ccaagaatac acagtttgtg
     1381 ttatgccact acgtggtgtc ccgggatccc agtgtctttc ctgagcccaa cagcttccag
     1441 cctcaccgat ggctgaggaa gaaagaggct gataaccctg ggatcctaca tccattcggc
     1501 tctgtgccct tcggctatgg ggttcggtct tgcctgggtc ggaggattgc agaactggag
     1561 atgcaactga tgctgtcaag gctggtacag aagtatgaga ttgccctggc tcccgggatg
     1621 ggagaagtaa agactgtgtc ccgcatcgtc ctggttccca gcaagaaggt gaggctgcat
     1681 tttctgcaga gacagtagta ccgagctggg ctcccgcctt catggagctc gtccagaagc
     1741 cctggcacag aagttcttgg ccagtctcat gtcacatgtc acgatgtcag attcaacagg
     1801 agaactctgt gcccttccta tagacaccaa atgtctggca caatctctac tgagcagcac
     1861 ccacttaaga cagcagagca ccttataata acagtccttg ggtatgattt aaaataaaat
     1921 ttaaaattca agtttt
//
