LOCUS       NM_009996               3311 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus cytochrome P450, family 24, subfamily a, polypeptide 1
            (Cyp24a1), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_009996
VERSION     NM_009996.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Meyer,M.B., Lee,S.M., Towne,J.M., Cichanski,S.R., Kaufmann,M.,
            Jones,G. and Pike,J.W.
  TITLE     In Vivo Contribution of Cyp24a1 Promoter Vitamin D Response
            Elements
  JOURNAL   Endocrinology 165 (11) (2024)
   PUBMED   39363152
  REMARK    GeneRIF: In Vivo Contribution of Cyp24a1 Promoter Vitamin D
            Response Elements.
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Tam,P.L.F., Cheung,M.F., Chan,L.Y. and Leung,D.
  TITLE     Cell-type differential targeting of SETDB1 prevents aberrant CTCF
            binding, chromatin looping, and cis-regulatory interactions
  JOURNAL   Nat Commun 15 (1), 15 (2024)
   PUBMED   38167730
  REMARK    GeneRIF: Cell-type differential targeting of SETDB1 prevents
            aberrant CTCF binding, chromatin looping, and cis-regulatory
            interactions.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3311)
  AUTHORS   Verma,A., Vincent-Chong,V.K., DeJong,H., Hershberger,P.A. and
            Seshadri,M.
  TITLE     Impact of dietary vitamin D on initiation and progression of oral
            cancer
  JOURNAL   J Steroid Biochem Mol Biol 199, 105603 (2020)
   PUBMED   31981799
  REMARK    GeneRIF: Impact of dietary vitamin D on initiation and progression
            of oral cancer.
REFERENCE   4  (bases 1 to 3311)
  AUTHORS   Meyer,M.B., Lee,S.M., Carlson,A.H., Benkusky,N.A., Kaufmann,M.,
            Jones,G. and Pike,J.W.
  TITLE     A chromatin-based mechanism controls differential regulation of the
            cytochrome P450 gene Cyp24a1 in renal and non-renal tissues
  JOURNAL   J Biol Chem 294 (39), 14467-14481 (2019)
   PUBMED   31439663
REFERENCE   5  (bases 1 to 3311)
  AUTHORS   Singh,A., Verma,A., Sallin,M.A., Lang,F., Sen,R. and Sen,J.M.
  TITLE     Noncoding variations in Cyp24a1 gene are associated with
            Klotho-mediated aging phenotypes in different strains of mice
  JOURNAL   Aging Cell 18 (3), e12949 (2019)
   PUBMED   30920112
  REMARK    GeneRIF: Decreased expression of Cyp24a1 in susceptible strains was
            associated with genetic alterations in noncoding regions of Cyp24a1
            gene, which were strongly reminiscent of super-enhancers that
            regulate gene expression.
REFERENCE   6  (bases 1 to 3311)
  AUTHORS   Akeno,N., Saikatsu,S., Kawane,T. and Horiuchi,N.
  TITLE     Mouse vitamin D-24-hydroxylase: molecular cloning, tissue
            distribution, and transcriptional regulation by
            1alpha,25-dihydroxyvitamin D3
  JOURNAL   Endocrinology 138 (6), 2233-2240 (1997)
   PUBMED   9165006
REFERENCE   7  (bases 1 to 3311)
  AUTHORS   Roy,S. and Tenenhouse,H.S.
  TITLE     Transcriptional regulation and renal localization of
            1,25-dihydroxyvitamin D3-24-hydroxylase gene expression: effects of
            the Hyp mutation and 1,25-dihydroxyvitamin D3
  JOURNAL   Endocrinology 137 (7), 2938-2946 (1996)
   PUBMED   8770917
REFERENCE   8  (bases 1 to 3311)
  AUTHORS   Itoh,S., Yoshimura,T., Iemura,O., Yamada,E., Tsujikawa,K.,
            Kohama,Y. and Mimura,T.
  TITLE     Molecular cloning of 25-hydroxyvitamin D-3 24-hydroxylase (Cyp-24)
            from mouse kidney: its inducibility by vitamin D-3
  JOURNAL   Biochim Biophys Acta 1264 (1), 26-28 (1995)
   PUBMED   7578252
REFERENCE   9  (bases 1 to 3311)
  AUTHORS   Williamson,C.M., Dutton,E.R., Abbott,C.M., Beechey,C.V., Ball,S.T.
            and Peters,J.
  TITLE     Thirteen genes (Cebpb, E2f1, Tcf4, Cyp24, Pck1, Acra4, Edn3, Kcnb1,
            Mc3r, Ntsr, Cd40, Plcg1 and Rcad) that probably lie in the distal
            imprinting region of mouse chromosome 2 are not monoallelically
            expressed
  JOURNAL   Genet Res 65 (2), 83-93 (1995)
   PUBMED   7781998
REFERENCE   10 (bases 1 to 3311)
  AUTHORS   Malas,S., Peters,J. and Abbott,C.
  TITLE     The genes for endothelin 3, vitamin D 24-hydroxylase, and
            melanocortin 3 receptor map to distal mouse chromosome 2, in the
            region of conserved synteny with human chromosome 20
  JOURNAL   Mamm Genome 5 (9), 577-579 (1994)
   PUBMED   8000144
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK159852.1.
            
            On Aug 20, 2015 this sequence version replaced NM_009996.3.
            
            Summary: The protein encoded by this gene localizes to the
            mitochondrion, where it degrades calcitriol to calcitetrol. This
            gene is upregulated by binding of calcitriol to the upstream
            regulatory region and to a downstream enhancer region, thereby
            allowing calcitriol to autoregulate its concentration in the cell.
            The encoded protein may also play a role in calcium homeostasis.
            [provided by RefSeq, Aug 2015].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK159109.1, AK159852.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3311              AK159852.1         2-3312
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 91.91 cM"
     gene            1..3311
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /note="cytochrome P450, family 24, subfamily a,
                     polypeptide 1"
                     /db_xref="GeneID:13081"
                     /db_xref="MGI:MGI:88593"
     exon            1..629
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     CDS             372..1916
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /EC_number="1.14.15.16"
                     /note="cytochrome P450, family 24, subfamily a,
                     polypeptide 1 (MGD|MGI:88593 GB|NM_009996, evidence:
                     BLASTN, 99%, match=3296); putative; cytochrome P450, 24a1;
                     1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial;
                     cytochrome P450 24A1; cytochrome P450-CC24; vitamin D(3)
                     24-hydroxylase; 25-hydroxyvitamin D-24-hydroxylase"
                     /codon_start=1
                     /product="1,25-dihydroxyvitamin D(3) 24-hydroxylase,
                     mitochondrial precursor"
                     /protein_id="NP_034126.1"
                     /db_xref="CCDS:CCDS17122.1"
                     /db_xref="GeneID:13081"
                     /db_xref="MGI:MGI:88593"
                     /translation="MSCPIDKRRPLIAFLRRLRDLGQPPRSVTSKAHVKRAPKEVPLC
                     PLMTDGETRNVTSLPGPTNWPLLGSLLEIFWKGGLKKQHDTLAEYHKKYGQIFRMKLG
                     SFDSVHLGSPSLLEALYRTESAHPQRLEIKPWKAYRDHRNEAYGLMILEGQEWQRVRS
                     AFQKKLMKPVEIMKLDKKINEVLADFMGQIDELRDERGRIQDLYSELNKWSFESICLV
                     LYEKRFGLLQKDTEEEALTFIAAIKTMMSTFGKMMVTPVELHKRLNTKVWQAHTLAWD
                     TIFKSVKPCIDHRLERYSQQPGADFLCDIYQQDHLSKKELYAAVTELQLAAVETTANS
                     LMWILYNLSRNPQVQQRLLREIQSVLPDNQTPRAEDVRNMPYLKACLKESMRLTPSVP
                     FTTRTLDKPTVLGEYTLPKGTVLTLNTQVLGSSEDNFEDADKFRPERWLEKEKKINPF
                     AHLPFGVGKRMCIGRRLAELQLHLALCWIIQKYNIVATDSEPVEMLHLGILVPSRELP
                     IAFCPR"
     exon            630..820
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            821..914
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            915..1011
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1103
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            1104..1215
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1361
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1528
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1607
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            1608..1805
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1925
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..3311
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3292..3297
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /note="putative"
     polyA_site      3311
                     /gene="Cyp24a1"
                     /gene_synonym="24-OHase; CP24; Cyp24"
                     /note="putative"
ORIGIN      
        1 ggggaggcac aaggaggtac atcagacctg gcggcatccc ttcgaccctc cttgacccct
       61 cgtggcttta gaccttagaa gtcaaatttg caaagccaca gagtactcgg gccaaacccc
      121 ggccgctctt ccccagccac tgtggccagc tcagggcagg gacctgcgcc ctaaacaact
      181 gcaccttgca gcatccctgg gcatctgcct cttccaagtc catcctgttg tacagtattt
      241 taccacaccc gcggcttctg tttgcctctc ttccccaaat cgccaaaact tcagaaccca
      301 acagcacttc cgaaatacct tacccatttc agtccctact gctcctcgag tgtcacgggt
      361 cgcctgctac tatgagctgc cccattgaca aaaggcgccc cctgatcgct ttcctgcgcc
      421 ggctgcggga cctcgggcaa cccccaaggt ccgtgacatc caaggcgcac gtgaagcgtg
      481 cgccaaaaga ggtgcccctc tgcccgctga tgaccgacgg tgagactcgg aacgtcacct
      541 ccttacctgg acccaccaac tggccactgc tgggcagcct actggagatt ttttggaaag
      601 gtggcctgaa gaaacagcac gacacactgg cagagtacca caagaagtat ggccagattt
      661 tccggatgaa gctgggctcc ttcgactcgg tgcatctggg ctcgccgagc ctgctggaag
      721 ctctgtaccg cacagagagc gcgcatcccc agcggctaga gatcaaaccc tggaaagcct
      781 atcgggacca tcgcaacgaa gcctacgggc tgatgatcct ggaaggacag gagtggcagc
      841 gggtccgcag cgccttccaa aagaaactca tgaagcccgt ggaaatcatg aagctggaca
      901 agaaaatcaa tgaggtcttg gctgatttta tggggcaaat cgatgagctg cgtgatgaga
      961 gaggccgcat ccaagacttg tacagcgagc tgaacaaatg gtcctttgaa agcatctgcc
     1021 ttgtgttata tgagaagaga ttcgggctcc ttcaaaagga cacagaggaa gaagccctga
     1081 ccttcattgc ggccatcaaa acaatgatga gcacatttgg gaagatgatg gtgacccccg
     1141 tggagctgca caagcgcctc aacaccaaag tgtggcaagc gcacacgctg gcctgggaca
     1201 ccattttcaa atcagtcaag ccctgcatcg accaccgcct agagagatat tcccagcagc
     1261 ctggtgcgga tttcctttgt gatatttatc agcaagatca tctttccaag aaagaactgt
     1321 acgctgctgt cacggagctc cagctggctg cagtggagac gaccgcaaac agcttgatgt
     1381 ggattctcta caatctatcc cggaatcccc aagtgcaaca gagacttctc cgggaaatcc
     1441 agagcgtgct gcctgacaac cagacgccac gggcggaaga tgtgaggaat atgccctatt
     1501 taaaggcctg tctaaaggag tccatgaggc ttaccccaag tgtgccattc acaactcgga
     1561 cccttgacaa gccaaccgtt ctgggtgaat acacgctacc caaaggaaca gtcttaacac
     1621 taaataccca agtgctgggc tctagcgaag acaattttga agatgctgac aagtttagac
     1681 ccgaacgctg gcttgaaaag gagaaaaaga tcaacccctt cgctcatctc ccatttggcg
     1741 tcgggaagag gatgtgcatc gggcgccggc tggctgagct acagctccac ttggctcttt
     1801 gctggataat ccaaaaatat aacattgtgg ctacagacag tgaacctgtg gagatgctgc
     1861 atcttggcat cctggtacct agccgggagc tgcccattgc gttctgtcca cggtaggctg
     1921 ctgagatttt ttttcacccc caaatcaaga ccagcaatct tcccccttct gcaagaaaac
     1981 tgcttatggc ttcggtattc ctagacggtg aacttcaggg accagagcat gggacaactg
     2041 cgaagactgg cacccaaagc cttctgcgga atgattctga aaacgatgtt tacaaagccc
     2101 attactcagg gaagcacatg gcggggcggg aatgccaggc gccgcccaca acactttacc
     2161 acgtgtttac aatagccaat cagagccctc aagagcctga tgtcactcca tcttctgtga
     2221 catcaccggg aggctgctgg tcagctactc tagtaaaggt tactaagtgt ttctaataca
     2281 aaagccaaag aggcagcttt aattcccatt tacccttgat gaagacctac tatggcttca
     2341 ttgtctgagt ggaactgaca aaccatttat tatactcggt tttgttcaat atttgaggaa
     2401 acgccagttg taaacagagc taaacattag gtaaaggctc tcataaagct acttttcctg
     2461 tttacaaggg gaaacatggt tggaaattag agactgtctg ctttaagaag gtctacagag
     2521 aagccagctc cttgcttacg aatgcctcgt tggtgcagag gactctgtca tttcttcttt
     2581 cgttggtacc tttctctgcg gctttagagt gtgcacagtc gaatcgacag tttctcagct
     2641 gtgtgtgtgt ccgcacatgt gtgtgccagc acatgcgtgt gagtggctgt gggccctggg
     2701 gtactttgct tcagtcatgt ttttccataa aggcataggg tacccaattt agggtctgca
     2761 gtgcggagct atgtcctgaa atgtagtcac agcttcgagg gatgcaccgc aagtgtaaga
     2821 agctgacttt ctgcgcctta atttctttat tcaaagtgat gattgtaaca tatgctcact
     2881 ccctatggat gcagtatgta tagtggggct tgagccccct gggtaagcac aatttcataa
     2941 acgcaaaaca cagttatgac ctactgacaa cgtttttaaa atgtttacat atggcttgtg
     3001 cattttgaaa ccagcactgt tttcctatag atgtttactg attatttcta tatacttggt
     3061 aaagacttcc cttttaggtt atggtcttag aatacttctg aaatacccat ttacatgtac
     3121 atttgaatga atctgtttgt atggctgaca caggcctatg ctttgtcttt tgtacaaaat
     3181 tatttttaga gttgtaaatc cagaggccaa agatgtagtt ctgtagtaag agtttttgct
     3241 tggtccatgt ggggccctga atttaatctc cagtactgcc aaataataaa aaataaaata
     3301 attacacctg t
//
