LOCUS       NM_009995               1939 bp    mRNA    linear   ROD 29-OCT-2024
DEFINITION  Mus musculus cytochrome P450, family 21, subfamily a, polypeptide 1
            (Cyp21a1), mRNA.
ACCESSION   NM_009995
VERSION     NM_009995.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Oikonomakos,I., Tedesco,M., Motamedi,F.J., Peitzsch,M., Nef,S.,
            Bornstein,S.R., Schedl,A., Steenblock,C. and Neirijnck,Y.
  TITLE     In vitro differentiation of mouse pluripotent stem cells into
            corticosteroid-producing adrenocortical cells
  JOURNAL   Stem Cell Reports 19 (9), 1289-1303 (2024)
   PUBMED   39178848
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1939)
  AUTHORS   Weniger,M., Mattes,M., Grunewald,T.G.P., Kohler,K., Hubner,A.,
            Beuschlein,F. and Reisch,N.
  TITLE     Quantitative Characterization of Ectopic Adrenal Gene Expression in
            Fetal Testes in 21-Hydroxylase Deficient Mice
  JOURNAL   Horm Metab Res 56 (1), 38-44 (2024)
   PUBMED   38171371
  REMARK    GeneRIF: Quantitative Characterization of Ectopic Adrenal Gene
            Expression in Fetal Testes in 21-Hydroxylase Deficient Mice.
REFERENCE   4  (bases 1 to 1939)
  AUTHORS   Uchida,A., Imaimatsu,K., Suzuki,H., Han,X., Ushioda,H., Uemura,M.,
            Imura-Kishi,K., Hiramatsu,R., Takase,H.M., Hirate,Y., Ogura,A.,
            Kanai-Azuma,M., Kudo,A. and Kanai,Y.
  TITLE     SOX17-positive rete testis epithelium is required for Sertoli valve
            formation and normal spermiogenesis in the male mouse
  JOURNAL   Nat Commun 13 (1), 7860 (2022)
   PUBMED   36543770
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1939)
  AUTHORS   Yadav,V.K., Berger,J.M., Singh,P., Nagarajan,P. and Karsenty,G.
  TITLE     Embryonic osteocalcin signaling determines lifelong adrenal
            steroidogenesis and homeostasis in the mouse
  JOURNAL   J Clin Invest 132 (4) (2022)
   PUBMED   34905510
REFERENCE   6  (bases 1 to 1939)
  AUTHORS   Ogata,R.T. and Zepf,N.E.
  TITLE     The murine Slp gene. Additional evidence that sex-limited protein
            has no biologic function
  JOURNAL   J Immunol 147 (8), 2756-2763 (1991)
   PUBMED   1918990
REFERENCE   7  (bases 1 to 1939)
  AUTHORS   Gaskins,H.R., Prochazka,M., Nadeau,J.H., Henson,V.W. and
            Leiter,E.H.
  TITLE     Localization of a mouse heat shock Hsp70 gene within the H-2
            complex
  JOURNAL   Immunogenetics 32 (4), 286-289 (1990)
   PUBMED   1978715
REFERENCE   8  (bases 1 to 1939)
  AUTHORS   Vincek,V., Kawaguchi,H., Mizuno,K., Zaleska-Rutczynska,Z.,
            Kasahara,M., Forejt,J., Figueroa,F. and Klein,J.
  TITLE     Linkage map of mouse chromosome 17: localization of 27 new DNA
            markers
  JOURNAL   Genomics 5 (4), 773-786 (1989)
   PUBMED   2574145
REFERENCE   9  (bases 1 to 1939)
  AUTHORS   Cortese Hassett,A.L., Radojcic,A., Watters,J.W., Locker,J.,
            Kunz,H.W. and Gill,T.J. 3rd.
  TITLE     Mapping and sequencing analysis of the rat MHC
  JOURNAL   Transplant Proc 21 (2), 3244-3246 (1989)
   PUBMED   2652811
  REMARK    Review article
REFERENCE   10 (bases 1 to 1939)
  AUTHORS   Nebert,D.W., Nelson,D.R., Adesnik,M., Coon,M.J., Estabrook,R.W.,
            Gonzalez,F.J., Guengerich,F.P., Gunsalus,I.C., Johnson,E.F.,
            Kemper,B. et al.
  TITLE     The P450 superfamily: updated listing of all genes and recommended
            nomenclature for the chromosomal loci
  JOURNAL   DNA 8 (1), 1-13 (1989)
   PUBMED   2651058
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK146565.1, BC127167.1, AI323066.1 and BQ033552.1.
            
            On Nov 22, 2007 this sequence version replaced NM_009995.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146565.1, BC127167.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849379 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1098              AK146565.1         138-1235
            1099-1491           BC127167.1         1072-1464
            1492-1885           AI323066.1         11-404
            1886-1939           BQ033552.1         1-54                c
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 18.36 cM"
     gene            1..1939
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /note="cytochrome P450, family 21, subfamily a,
                     polypeptide 1"
                     /db_xref="GeneID:13079"
                     /db_xref="MGI:MGI:88591"
     exon            1..229
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..1491
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /EC_number="1.14.14.16"
                     /note="cytochrome P450, family 21, subfamily a,
                     polypeptide 2, pseudogene; cytochrome P-450; 21-OHase;
                     cytochrome P450 21; cytochrome P-450c21; cytochrome P450
                     XXI; cytochrome P450-C21; cytochrome P450, 21, steroid 21
                     hydroxylase; cytochrome P450, 21, pseudogene; cytochrome
                     P450 hydroxylase A; 21-hydroxylase"
                     /codon_start=1
                     /product="steroid 21-hydroxylase"
                     /protein_id="NP_034125.2"
                     /db_xref="CCDS:CCDS28658.1"
                     /db_xref="GeneID:13079"
                     /db_xref="MGI:MGI:88591"
                     /translation="MLLPGLLLLLLLLAGTRWLWGQWKLRKLHLPPLAPGFLHFLQPN
                     LPIYLLGLTQKLGPIYRIRLGMQDVVVLNSNRTIEEALIQKWVDFAGRPHMLNGKMDL
                     DLSLGDYSLMWKAHKKLSRSALMLGMRDSMEPLIEQLTQEFCERMRAQAGTPVAIHKE
                     FSFLTCSIISCLTFGDKDSTLVQTLHDCVQDLLQAWNHWSIQILTIIPLLRFLPNPGL
                     QKLKQIQESRDHIVKQQLKRHKDSLVAGQWKDMIDYMLQGVEKQRDGKDEERLHEGHV
                     HMSVVDLFIGGTETTATTLSWAVAFLLHHPEIQKRLQEELDLKLGPGSQLLYRNRMQL
                     PLLMATIAEVLRLRPVVPLALPHRATRASSISGYDIPKDMVIIPNIQGANLDEMVWEL
                     PSKFWPDRFLEPGKNPRTPSFGCGARVCLGEPLARLELFVVLARLLQAFTLLPPPDGT
                     LPSLQPQPYAGINLPIPPFQVRLQPRNLAPQDQGERP"
     exon            230..319
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     exon            320..462
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     exon            463..558
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     exon            559..660
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     exon            661..747
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     exon            748..948
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1118
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1222
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1923
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1893..1898
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /note="hexamer: AATAAA"
     polyA_site      1923
                     /gene="Cyp21a1"
                     /gene_synonym="21-OH; 21OH; 21OHA; 21OHB; Cyp21;
                     Cyp21-ps1; Cyp21a2-ps; Cyp21a2ps; Cyp21B; CYP21OH-A;
                     Oh21-1; Oh21-2"
                     /note="major polyA site"
ORIGIN      
        1 atcagggccc tgcacaagtg ctgggccatg ctgctacctg ggctgctgct gctgttgctg
       61 ctgctagctg gcacccgctg gctgtggggc caatggaagc tccggaagct gcacctcccg
      121 cctctggccc cgggttttct gcacttccta cagcctaacc ttcccatcta cctgcttggc
      181 ctcactcaga aactcgggcc catctacagg atccgcttgg ggatgcaaga tgtggtggtg
      241 ctaaattcta acagaaccat tgaggaggcc ttgatccaaa agtgggtgga ctttgctggc
      301 cgaccccata tgctaaatgg aaagatggac ttggacctgt ccctggggga ttactctctc
      361 atgtggaagg cccacaagaa actctctcgc tcagccctga tgctgggcat gcgagactcc
      421 atggagcctc tgatagagca gctgacccag gagttctgtg agcgcatgcg agcccaggct
      481 ggcacccccg tggccatcca taaggaattc tccttcctca cttgtagtat catctcctgc
      541 ctcacttttg gagacaagga cagcacgttg gtacagaccc ttcacgactg tgtccaggac
      601 ttgttgcaag cctggaacca ctggtccatc caaatcttga cgataattcc ccttctcagg
      661 ttcctcccca acccaggcct ccagaagctg aagcagatcc aagagagtcg ggaccatatt
      721 gtcaagcagc agctgaagcg gcacaaggac agcctggttg caggccaatg gaaagacatg
      781 attgactaca tgctccaggg agtggagaag caaagggatg gcaaagacga agagcggctc
      841 cacgaggggc acgtgcacat gtcggtggtg gacctgttca tcggcggcac cgagaccacg
      901 gctaccacgc tctcctgggc tgtggctttc ctgcttcacc accctgagat ccagaagcga
      961 ctgcaggaag agttagacct caagctgggc ccaggctccc agctcctgta caggaaccga
     1021 atgcagctgc ctctgctcat ggccaccatt gccgaggtgc tgcgtttgcg gcctgtggtg
     1081 cccttggcct tgccccatcg tgcaactagg gctagcagca tctccggcta tgacatccct
     1141 aaggatatgg tcatcatccc caacatccaa ggcgccaacc tggatgagat ggtttgggaa
     1201 ctgcccagca agttctggcc agatcgcttc ctggaacctg ggaagaatcc cagaacacca
     1261 tcctttggct gtggggcacg cgtgtgcctg ggagagcctc tggcacggct ggagctcttt
     1321 gtggtgctgg ctcgtctgct gcaggccttc actctgctgc ctccaccaga tggaaccctg
     1381 ccttccctgc agccccagcc ttatgctggc atcaatctcc cgattcctcc tttccaggtg
     1441 cggctgcagc ccagaaacct ggcgccccaa gaccagggtg agcgtccttg acaggatagg
     1501 acgagtctct ttaaagtttc tcctttattg ctcagttccc cccccccccc cgtaaacatg
     1561 gtgctgtgag attgtggcag agaaggcttc ctcggtgagg cacggtggct cttctctact
     1621 ggggtgtgag tgaggtaggc agcaggctca ggtgcccctg gtgggggcct ggaagtttct
     1681 gggtcggagc ttcatttccg tgaagggcac agaaaactcg aagcccttcc agtggtacca
     1741 gctcactccc tgaggagagg gttgtcaagg gaagagagtc aagacagcgc catatcaatg
     1801 ccacctaatc cagccccacc ctgctcctgt aggtgactcc cagggtccag ctatctctcc
     1861 catgatccct gagctggtgc cttctgccca gtaataaatg gtctcttcag atttcctcag
     1921 caaaaaaaaa aaaaaaaaa
//
