LOCUS       NM_030013               2274 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cytochrome P450, family 20, subfamily a, polypeptide 1
            (Cyp20a1), transcript variant 1, mRNA.
ACCESSION   NM_030013 XM_129747
VERSION     NM_030013.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2274)
  AUTHORS   Choudhary,D., Jansson,I., Schenkman,J.B., Sarfarazi,M. and
            Stoilov,I.
  TITLE     Comparative expression profiling of 40 mouse cytochrome P450 genes
            in embryonic and adult tissues
  JOURNAL   Arch Biochem Biophys 414 (1), 91-100 (2003)
   PUBMED   12745259
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC158139.11, AK053388.1 and BC049147.1.
            
            On Aug 27, 2015 this sequence version replaced NM_030013.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7345562.5083716.1, AK053388.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                AC158139.11        192871-192942
            73-1573             AK053388.1         4-1504
            1574-2274           BC049147.1         1319-2019
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 30.47 cM"
     gene            1..2274
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /note="cytochrome P450, family 20, subfamily a,
                     polypeptide 1"
                     /db_xref="GeneID:77951"
                     /db_xref="MGI:MGI:1925201"
     exon            1..158
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..1475
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cytochrome P450, 20a1; cytochrome P450 monooxygenase"
                     /codon_start=1
                     /product="cytochrome P450 20A1 isoform 1"
                     /protein_id="NP_084289.1"
                     /db_xref="CCDS:CCDS14990.1"
                     /db_xref="GeneID:77951"
                     /db_xref="MGI:MGI:1925201"
                     /translation="MLDFAIFAVTFLLALVGAVLYLYPASRQASGIPGLTPTEEKDGN
                     LPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTTDVLKQHFNPNKTSDPFET
                     MLKSLLGYQSGGGSAGEDHVRRKLYGDAVTASLHSNFPLLLQLSEELLDKWLSYPETQ
                     HIPLSQHMLGFALKFVTRMVLGSTFEDEQEVIRFQKIHGTVWSEIGKGFLDGSLDKNT
                     TRKKQYQEALMQLESTLKKIIKERKGGNFRQHTFIDSLTQGKLNEQQILEDCVVFSLA
                     SCIITARLCTWTIHFLTTTGEVQKKLCKEIDQVLGEGPITSEKIEQLSYCQQVLFETV
                     RTAKLTPVSARLQDIEGKVGPFVIPKETLVLYALGVVLQDPSTWPLPHRFDPDRFADE
                     PVMKVFSSLGFSGTWECPELRFAYMVTAVLVSVLLKRLRLLAVDRQVFEMKYELVTSA
                     REEAWITVSKRH"
     exon            159..208
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            209..375
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            376..518
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            519..686
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            687..765
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            766..881
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            882..936
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1057
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1169
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1234
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1324
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     exon            1325..2246
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2226..2231
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /note="hexamer: AATAAA"
     polyA_site      2246
                     /gene="Cyp20a1"
                     /gene_synonym="A930011N14Rik; Cypm"
                     /note="major polyA site"
ORIGIN      
        1 gaccgcctga ggccacctcc ctccccagct gcgggcgcca ccacggagct cacggccgag
       61 gaccgcgcgg gcgggcgaga ggaagcatgc tggacttcgc catcttcgcc gtgacgttcc
      121 tgctggcgct ggtgggagcg gtgctgtacc tctacccggc ttcccggcaa gcttcaggaa
      181 ttccagggct tactccaact gaagaaaaag atggtaatct tccagacatt gtaaacagtg
      241 gaagtttgca tgagttcctg gttaatttgc atgaaagata cgggcctgtg gtctctttct
      301 ggtttggcag gcgtcttgtg gttagcttgg gcaccactga tgtactgaag caacatttca
      361 accccaataa gacttcggac ccatttgaaa ccatgttgaa gtcattatta gggtatcagt
      421 ctggcggagg gagtgcgggt gaggaccacg tgaggaggaa actgtatgga gatgccgtga
      481 cagcttctct gcatagtaac ttccctctcc tgctacagct ttcagaagaa ttgttagata
      541 aatggctgtc ctatccagag acccagcaca tacctctgag ccagcatatg ctcggttttg
      601 ctttgaagtt tgtcacaagg atggtactgg gtagcacctt tgaagatgaa caggaggtca
      661 tccgattcca aaagattcat ggcacagtgt ggtctgagat tggaaaaggc tttctagatg
      721 ggtcactcga taaaaacacc actcggaaaa aacagtatca agaggctctc atgcaattgg
      781 aatccacttt aaagaaaatc ataaaggaac gaaaaggagg gaacttcagg caacacacct
      841 tcatcgactc cttaacacag ggaaaactta atgagcaaca gatcctagag gactgtgtgg
      901 tgttctctct ggccagctgc ataataactg caaggttgtg tacctggacg atccatttcc
      961 ttacgacgac tggagaagtt cagaagaagc tatgcaagga gatagaccaa gttttagggg
     1021 agggtcctat cacttcagag aaaattgagc agctcagtta ttgtcagcaa gtactttttg
     1081 aaactgttcg gactgctaaa ctaactccag tttctgcccg gcttcaagac attgaaggaa
     1141 aagtcggccc atttgttatt cctaaggaga cactcgtcct ttatgccctt ggcgtggttc
     1201 ttcaggaccc cagcacttgg ccattaccac acaggtttga tccagaccgc tttgctgacg
     1261 agccagtgat gaaagtgttt tcttctcttg gcttctcagg cacatgggaa tgtccagaac
     1321 tgaggtttgc ttacatggtg accgcagtgc ttgtgagtgt gttgctgaag aggctgcgcc
     1381 tgctcgctgt ggacagacaa gtttttgaaa tgaagtatga actggtcaca tcagcaaggg
     1441 aagaagcttg gatcactgtc tccaaaagac actaaacgtt tatgcactta aagtctgtta
     1501 ggtaaatgaa gggaaatgcc cagttagagt atgtgctgag accttttata aacccagtat
     1561 cattttgtaa tgtaaaacct gctagtgctt catgatgtaa atttgttttt tggttttttt
     1621 gttttgtttt gttttgtttt ttgtttttcg agacagggtt tctctgtgta gccctggctg
     1681 tcctggaact cactttgtag accaggctgg cctcgaactc agaaatccgc ctgcctctgc
     1741 ctcccgagtg ctgggattaa aggcgtgcgc caccacgcct ggctaaattt gggtttttat
     1801 atgtcaaaat ttcttactgt atgacatatg tttatactta taatgacaac tttaacagga
     1861 aagtttaatt ttcttttaat ttgcatcatt ctctaaaacc attattttta aatttttatt
     1921 atcatttgtg gggcagtggg ttttgagaca gggttttttc tgtgtagccc tgactgctct
     1981 gtaactcact ctgtagacca gactggcctc aaactcatag agatccacct gcctctgcct
     2041 ccagagagct aggatcaaag gcctgcatca ctactacctg gctaagctgt tattttcaat
     2101 cttgatgttc ataatttgac ctctcaaatg agtaatctta gagagttctt ttgtgcaagt
     2161 tggagaatca tgttttctaa atattcataa aagagaaaca taatgttgct tctttttgca
     2221 tttctaataa atcacctatc actctaaaaa aaaaaaaaaa aaaaaaaaaa aaaa
//
