LOCUS       NM_173369               8014 bp    mRNA    linear   ROD 13-MAY-2024
DEFINITION  Mus musculus CYLD lysine 63 deubiquitinase (Cyld), transcript
            variant 2, mRNA.
ACCESSION   NM_173369
VERSION     NM_173369.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Pseftogas,A., Xanthopoulos,K., Siasiaridis,A., Poutahidis,T.,
            Gonidas,C., Tsingotjidou,A., Hatzivassiliou,E. and Mosialos,G.
  TITLE     Inactivation of the Tumor Suppressor CYLD Sensitizes Mice to Breast
            Cancer Development
  JOURNAL   Anticancer Res 44 (5), 1885-1894 (2024)
   PUBMED   38677721
  REMARK    GeneRIF: Inactivation of the Tumor Suppressor CYLD Sensitizes Mice
            to Breast Cancer Development.
REFERENCE   2  (bases 1 to 8014)
  AUTHORS   Jiang,G., Cai,Y., Cheng,D., Wang,H., Deng,G. and Xiang,D.
  TITLE     CYLD alleviates NLRP3 inflammasome-mediated pyroptosis in
            osteoporosis by deubiquitinating WNK1
  JOURNAL   J Orthop Surg Res 19 (1), 212 (2024)
   PUBMED   38561786
  REMARK    GeneRIF: CYLD alleviates NLRP3 inflammasome-mediated pyroptosis in
            osteoporosis by deubiquitinating WNK1.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8014)
  AUTHORS   Wu,M., Zhao,J., Wu,W., Hao,C., Yang,Y. and Zhang,J.
  TITLE     miR-130b regulates B cell proliferation via CYLD-mediated NF-kappaB
            signaling
  JOURNAL   Exp Cell Res 434 (1), 113870 (2024)
   PUBMED   38049082
  REMARK    GeneRIF: miR-130b regulates B cell proliferation via CYLD-mediated
            NF-kappaB signaling.
REFERENCE   4  (bases 1 to 8014)
  AUTHORS   Han,Y.Y., Zhou,J.W., Guo,Z.W., Wu,Z.Q., Zhang,Z.Y., Liu,D.X. and
            Long,C.
  TITLE     Multiple brain regions are involved in reaction to acute restraint
            stress in CYLD-knockout mice
  JOURNAL   Stress 26 (1), 2228925 (2023)
   PUBMED   37395260
  REMARK    GeneRIF: Multiple brain regions are involved in reaction to acute
            restraint stress in CYLD-knockout mice.
REFERENCE   5  (bases 1 to 8014)
  AUTHORS   Jin,W., Reiley,W.R., Lee,A.J., Wright,A., Wu,X., Zhang,M. and
            Sun,S.C.
  TITLE     Deubiquitinating enzyme CYLD regulates the peripheral development
            and naive phenotype maintenance of B cells
  JOURNAL   J Biol Chem 282 (21), 15884-15893 (2007)
   PUBMED   17392286
  REMARK    GeneRIF: a critical role for CYLD in regulating the basal activity
            of NF-kappaB and maintaining the naive phenotype and proper
            activation of B cells.
REFERENCE   6  (bases 1 to 8014)
  AUTHORS   Zhang,J., Stirling,B., Temmerman,S.T., Ma,C.A., Fuss,I.J.,
            Derry,J.M. and Jain,A.
  TITLE     Impaired regulation of NF-kappaB and increased susceptibility to
            colitis-associated tumorigenesis in CYLD-deficient mice
  JOURNAL   J Clin Invest 116 (11), 3042-3049 (2006)
   PUBMED   17053834
  REMARK    GeneRIF: CYLD limits inflammation and tumorigenesis by regulating
            ubiquitination in vivo
REFERENCE   7  (bases 1 to 8014)
  AUTHORS   Massoumi,R., Chmielarska,K., Hennecke,K., Pfeifer,A. and Fassler,R.
  TITLE     Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent
            NF-kappaB signaling
  JOURNAL   Cell 125 (4), 665-677 (2006)
   PUBMED   16713561
  REMARK    GeneRIF: These data indicate that, depending on the external
            signals, Cyld can negatively regulate different NF-kappaB pathways;
            inactivation of TRAF2 controls survival and inflammation, while
            inhibition of Bcl-3 controls proliferation and tumor growth.
REFERENCE   8  (bases 1 to 8014)
  AUTHORS   Reiley,W.W., Zhang,M., Jin,W., Losiewicz,M., Donohue,K.B.,
            Norbury,C.C. and Sun,S.C.
  TITLE     Regulation of T cell development by the deubiquitinating enzyme
            CYLD
  JOURNAL   Nat Immunol 7 (4), 411-417 (2006)
   PUBMED   16501569
  REMARK    GeneRIF: identifed CYLD as a positive regulator of proximal T cell
            receptor signaling in thymocytes
REFERENCE   9  (bases 1 to 8014)
  AUTHORS   Jono,H., Lim,J.H., Chen,L.F., Xu,H., Trompouki,E., Pan,Z.K.,
            Mosialos,G. and Li,J.D.
  TITLE     NF-kappaB is essential for induction of CYLD, the negative
            regulator of NF-kappaB: evidence for a novel inducible
            autoregulatory feedback pathway
  JOURNAL   J Biol Chem 279 (35), 36171-36174 (2004)
   PUBMED   15226292
  REMARK    GeneRIF: CYLD is induced by NF-kappaB
REFERENCE   10 (bases 1 to 8014)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC156937.4 and AC138025.4.
            
            On Jan 20, 2013 this sequence version replaced NM_173369.2.
            
            Summary: This gene encodes a protein that is a member of the
            ubiquitin C-terminal hydrolase subfamily of the deubiquitinating
            enzyme family. Members of this family catalyze the removal of
            ubiquitin from a substrate or another ubiquitin molecule and
            thereby play important roles in regulating signaling pathways,
            recycling ubiquitin and regulating protein stability. This protein
            removes ubiquitin from K-63-linked ubiquitin chains from proteins
            involved in NF-kappaB signaling and thus acts as a negative
            regulator of this pathway. In humans mutations in this gene have
            been associated with cylindromatosis, an autosomal dominant
            predisposition to tumors of skin appendages. In mouse deficiency of
            this gene impairs thymocyte development and increases
            susceptibility to skin and colon tumors. A pseudogene of this gene
            has been identified on chromosome 1. Alternative splicing results
            in multiple transcript variants that encode different protein
            isoforms. [provided by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (2) encodes isoform b. Both
            variants 2 and 4 encode isoform b.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC042438.1, AK133479.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                AC156937.4         53838-53909         c
            73-726              AC156937.4         45058-45711         c
            727-1029            AC156937.4         43546-43848         c
            1030-1135           AC156937.4         40941-41046         c
            1136-1234           AC156937.4         31541-31639         c
            1235-1348           AC156937.4         27725-27838         c
            1349-1728           AC156937.4         21099-21478         c
            1729-1894           AC156937.4         20116-20281         c
            1895-2036           AC156937.4         19121-19262         c
            2037-2159           AC156937.4         17829-17951         c
            2160-2251           AC156937.4         15963-16054         c
            2252-2318           AC156937.4         15040-15106         c
            2319-2451           AC156937.4         9514-9646           c
            2452-2560           AC156937.4         8543-8651           c
            2561-2679           AC156937.4         5991-6109           c
            2680-2896           AC156937.4         5512-5728           c
            2897-7004           AC156937.4         1-4108              c
            7005-8014           AC138025.4         69605-70614         c
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 43.51 cM"
     gene            1..8014
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /note="CYLD lysine 63 deubiquitinase"
                     /db_xref="GeneID:74256"
                     /db_xref="MGI:MGI:1921506"
     exon            1..72
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            73..726
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     CDS             223..3081
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /EC_number="3.4.19.12"
                     /note="isoform b is encoded by transcript variant 2;
                     ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin
                     thioesterase CYLD; deubiquitinating enzyme CYLD;
                     ubiquitin-specific-processing protease CYLD; ubiquitin
                     thiolesterase CYLD; cylindromatosis (turban tumor
                     syndrome)"
                     /codon_start=1
                     /product="ubiquitin carboxyl-terminal hydrolase CYLD
                     isoform b"
                     /protein_id="NP_775545.1"
                     /db_xref="CCDS:CCDS22513.1"
                     /db_xref="GeneID:74256"
                     /db_xref="MGI:MGI:1921506"
                     /translation="MSSGLWSQEKVTSPYWEERIFYLLLQECSVTDKQTQKLLKVPKG
                     SIGQYIQDRSVGHSRVPSTKGKKNQIGLKILEQPHAVLFVDEKDVVEINEKFTELLLA
                     ITNCEERLSLFRNRLRLSKGLQVDVGSPVKVQLRSGEEKFPGVVRFRGPLLAERTVSG
                     IFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDNGLESDFAG
                     PGDTMQVEPPPLEINSRVSLKVGESTESGTVIFCDVLPGKESLGYFVGVDMDNPIGNW
                     DGRFDGVQLCSFASVESTILLHINDIIPDSVTQERRPPKLAFMSRGVGDKGSSSHNKP
                     KVTGSTSDPGSRNRSELFYTLNGSSVDSQQSKSKNPWYIDEVAEDPAKSLTEMSSDFG
                     HSSPPPQPPSMNSLSSENRFHSLPFSLTKMPNTNGSMAHSPLSLSVQSVMGELNSTPV
                     QESPPLPISSGNAHGLEVGSLAEVKENPPFYGVIRWIGQPPGLSDVLAGLELEDECAG
                     CTDGTFRGTRYFTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSLAFGGYLSEV
                     VEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSALDTVLLRPKEKN
                     DIEYYSETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFL
                     NILFHDILRVEPLLKIRSAGQKVQDCNFYQIFMEKNEKVGVPTIQQLLEWSFINSNLK
                     FAEAPSCLIIQMPRFGKDFKLFKKIFPSLELNITDLLEDTPRQCRICGGLAMYECREC
                     YDDPDISAGKIKQFCKTCSTQVHLHPRRLNHSYHPVSLPKDLPDWDWRHGCIPCQKME
                     LFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKM
                     SLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK"
     exon            727..1029
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1135
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1234
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            1235..1348
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            1349..1728
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            1729..1894
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..2036
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2159
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2251
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            2252..2318
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            2319..2451
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            2452..2560
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2679
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            2680..2896
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     exon            2897..8014
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4529..4534
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /note="hexamer: TATAAA"
     polyA_site      4557
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /note="major polyA site"
     regulatory      4608..4613
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /note="hexamer: TATAAA"
     polyA_site      4628
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /note="major polyA site"
     regulatory      7990..7995
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
                     /note="hexamer: ATTAAA"
     polyA_site      8013
                     /gene="Cyld"
                     /gene_synonym="2010013M14Rik; 2900009M21Rik;
                     C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849"
ORIGIN      
        1 agttcggagg cggggcaggt gggggcgggc ccaggtagca ggttcggctg cgcgggggcc
       61 cgcgcgcctg aggcactttg aattgctgtc tttttacaac atggatgcca ggttgctaaa
      121 atcttgcttt gggacttaca gcgagttcat ttttttggat tttatgacag atttactagt
      181 ggctcctttt tgccagtcaa tgttctgaaa gttactgtca caatgagttc aggcctgtgg
      241 agccaagaga aagttacttc accctactgg gaagaacgga ttttttatct gcttcttcaa
      301 gaatgcagtg taacagacaa acaaactcag aagctgctga aagtacccaa agggagtata
      361 ggacagtaca tccaagaccg ttctgtgggg cattcaagag ttccttccac aaaaggcaag
      421 aaaaatcaga ttggattaaa aatcttggag caaccgcatg cagttctgtt tgttgatgaa
      481 aaggatgttg tagaaataaa tgaaaaattc acagagttac tgttggcaat taccaactgt
      541 gaggagaggc tcagcctatt tagaaacaga ctccgactaa gtaaaggcct ccaggtagac
      601 gtgggcagtc ctgtgaaagt acagctgcga tctggggaag agaaatttcc aggagttgta
      661 cgcttcagag gacctttatt agcggagagg acggtgtcgg ggattttctt tggagtagaa
      721 ttattggaag aaggtcgtgg tcaaggtttc acggatgggg tatatcaagg gaagcagctt
      781 ttccagtgtg atgaggactg tggcgtgttt gttgcattgg acaaactaga acttatagaa
      841 gatgatgaca atggattgga aagtgatttt gcaggcccag gagatacaat gcaggttgaa
      901 cctccccctt tggaaataaa ctccagagtt tctttgaagg ttggagaaag tacagaatct
      961 ggaacagtaa tattctgtga tgttttacca ggaaaagaga gtctaggata ttttgttggt
     1021 gtggacatgg ataaccctat tggcaactgg gatggaaggt ttgatggagt acagctctgt
     1081 agttttgcaa gtgttgaaag tacaattctc ctgcacatca atgacatcat cccagatagc
     1141 gtgacacagg aaaggaggcc tcccaaactt gcctttatgt caagaggtgt aggtgacaaa
     1201 ggttcatcta gtcataataa accaaaggtt acaggatcta cctcagaccc tggaagtaga
     1261 aacagatctg aattatttta taccttaaat gggtcatctg ttgactcaca acaatccaag
     1321 tccaaaaatc catggtacat tgatgaagtt gcagaagacc ctgcaaagtc acttacagag
     1381 atgtcttcgg acttcggaca ttcatctcct ccaccgcagc ctccttccat gaactccttg
     1441 tctagcgaga acagattcca ctccttaccc ttcagcctga caaagatgcc caatactaat
     1501 ggcagcatgg ctcatagtcc actctctctg tcagtgcagt ctgtgatggg ggagctgaac
     1561 agcacacctg tccaggagag tccacccttg cccatctctt ctgggaatgc acacgggcta
     1621 gaggtgggct cactggctga agtaaaagag aaccccccgt tctatggggt tatccgttgg
     1681 attggccagc caccagggct cagtgacgtg ctagctggac tggaactgga agatgaatgc
     1741 gcaggctgta cagatggaac tttcaggggc acgcggtatt tcacgtgtgc cctgaagaag
     1801 gcactgtttg tgaaactgaa gagctgcaga ccggactcta ggtttgcatc cttgcagcct
     1861 gtttccaatc aaattgaaag gtgtaactct ttagcatttg ggggctattt aagtgaagta
     1921 gtagaagaaa atactccacc taaaatggaa aaggaaggtt tagagataat gattggaaag
     1981 aagaaaggca tccagggcca ttacaattct tgttacttag actcaacttt attctgctta
     2041 tttgctttta gttctgccct ggacactgtg ttacttagac ccaaagagaa gaatgatata
     2101 gagtattaca gtgagactca ggagctactg aggacagaga tagtcaatcc tctgagaata
     2161 tatggatatg tgtgtgccac aaagattatg aaactgagga aaatacttga aaaagttgag
     2221 gctgcatcag gttttacctc tgaagaaaaa gatcctgaag aattcttaaa tatcctgttt
     2281 catgatattt taagggttga accattgtta aaaataagat cagcaggtca aaaagttcaa
     2341 gactgtaact tctatcaaat ttttatggaa aaaaatgaga aagttggagt acccacaatt
     2401 cagcagttat tagaatggtc ttttatcaac agcaacctga aatttgcaga ggcaccatca
     2461 tgcttgatta tccagatgcc tcggtttgga aaagacttta aactatttaa aaaaattttt
     2521 ccttccctgg aattaaatat aacagattta cttgaagaca ctcccaggca gtgccgcatc
     2581 tgtggaggac ttgcgatgta cgagtgcagg gagtgctatg acgatccgga catctcagct
     2641 gggaagatca agcagttctg taagacctgc agcactcagg ttcaccttca tcccaggagg
     2701 ttgaatcatt cttatcatcc agtatcactt cccaaagact tgcctgactg ggactggaga
     2761 cacggctgca tcccctgtca gaagatggag ttatttgctg ttctctgcat agaaacgagc
     2821 cactatgttg cttttgtgaa gtatgggaag gatgactctg cctggctttt ctttgacagc
     2881 atggcggatc gagatggtgg tcagaatggc ttcaacattc cacaagtgac gccctgccca
     2941 gaagtgggag agtacttgaa gatgtctctg gaggacctgc actctttgga ttccagaagg
     3001 attcaaggct gtgcgcgcag acttctttgc gatgcataca tgtgcatgta ccagagtcca
     3061 accatgagcc tgtacaaata accaggttat ccgcagtggg cagagacagc acaaacctta
     3121 gtgctgtgtc ttcctccagc ttgtggttct gtccggcacc cattgccggc aatgggtgtc
     3181 tcttttgatg atggcccttc agcaaaggat tcctgtgttt ccaaacaaat tgcttttgtg
     3241 ttcctgaagt atttaataag aagcattttg cactctagga agtatgtttg tgttgatttt
     3301 ttaagaagtc taaatggatt tattaatatc tgacacttta agttaactgc attgatgata
     3361 cgatattttt ggaagcatac aattttaatt gtgacgtcta aaacctcttt tagtccattg
     3421 aggatgtaaa taaatatttc ttcttatgga ccagggatat gaaatcattt tccttttgta
     3481 gctaatgatt gccttgagaa agacataata tgattttatt aagagtctgc ctttactcca
     3541 attgttgaag gtgtgctgag tttaattgtt aattttccta tatagtgctg atttcctgca
     3601 tgtctgtact ttgctgagtt tctgtgttcc tgcagcagtg gtcagaacat gctgaaattc
     3661 ctttcatggt gttatttttt atttgttcta gttttgagat aaatgagtga ctctgtcata
     3721 aaatgtccat ttttgatttg ttcttgaaga attagggtac aaaacaagta tcgtagtcat
     3781 agtaacaact gttactgaac aggtcttctg taggggctcc taagtatgag acaacatgca
     3841 gtggtttctg aaatgactga gcgataagta aattatatat tcttttttta aaagatttat
     3901 tttatttata tgagtacact gtagctgtct tcagacacac cagaagaggg catcagatct
     3961 tattacagat ggttgtgagc caccatgtgg ttgctgggaa ttgaactcag gacctttgga
     4021 agggcagtca gtgctcttaa ctgctgagct atctctctag ccccaaatta tatattctta
     4081 tacagaccca cagtccagag aaagtgaagc tggctcagag ttagcaccaa catttccctg
     4141 aaaaaatgtg ttaggtgttt aaaaatctca tcgtaaataa attattttat ttttagttta
     4201 agtgaatttc tcttaattta aataagacta atatttttgt ttgactgaag acccttgtac
     4261 tctctggtgt cagtttggtg tcacttgaaa caatgacaac cacgtcagca tgttactgca
     4321 atgtcagtga tggtttcaaa ggagaaaatg gatgtcatct taggactgct tgtgtgtgta
     4381 tagaccgtgg tggatgctgt agagtgcttg ttgtctgagt gttgttttgt ggtatggctg
     4441 tctctctgaa taatgtcaac cttacagaaa gagaaggtgt tatatgtgta acatatggct
     4501 ttataaaaga tttaatgtca aaagagtcta taaatattgc tgttattttt aaaggcactt
     4561 tactccttgg ttttattatt ccattttgct ggcgtgtatt agctttatat aaatcacaac
     4621 acacaaaact cgtccatctt gtaatctttg tacttttgca atggaaaagt gtgtatactg
     4681 tgcacagact tttatatagc tgagtgccta gactgggtgg agaggccatg agtagatagc
     4741 agtaaagtcc tccagctata ggctagcctt gtcttcatca tcctgggaca ggtgagaacg
     4801 gtgagcacag gtgagtctga gcttgcacac tgaccagaat tcagtcaccc tgtaataagt
     4861 atgtatttaa tgagtctgtt agtgtggtag tgtgattttg aaaggtttaa ctaaggaatg
     4921 gtgaggtctt tgtttatagg caagcctggc ttaacagtga aggggagggg gctgagggaa
     4981 agcttttttt ttttttaatt tcagaaatgg aagcaaaatc catgtgcaaa gagactagct
     5041 acttggcttt cagagcagag gactcaaagc ttgtttgcag tggttctgaa gttggggtgg
     5101 cactttgtga gtgaggagga ccatgtccac aggcaatccc taagcaagat cgtctcgaaa
     5161 ttgcactgtg ttctttagca gtagtggctg ggtaggggca cacagcggaa cacccacttg
     5221 cagtggctgg ggaggcctga gaagcacagg ccagcgggag ctctctccca tgccttcttg
     5281 tccctctgga cagcccctcc tcattccttg tcccagcaga gcctggtttg cccctggggc
     5341 aatagggttg gactgtgtgg tgcagatagc cctcgtgcct gtggattact ttaattttgt
     5401 aatcttaggt tttctgtacg gttttcccat tgccttttaa aatattttga gtgtccttct
     5461 ccttatcaag atggtagtgt agtaggattg ttttactcac aacatggttt aggagccata
     5521 gctacaaata ggaaatataa gaattctatt tctctactgt gaggcaaatc tgtatatatg
     5581 tatggagagt caggaggatg aacaagggag aggaaagggg cccaggcaca tttttaatcc
     5641 aaaagctaca aagtgtcgag ctcctcaggg ccagcttcac agagagtcca gctcagttca
     5701 caaagtattt tctaagttgg ggaaatgcat tgagaaacag agcgagatat ctgtgtagtg
     5761 cagctgtggg tggttgctat gcattttatc ctcagagtag cctggggttt ttattttatg
     5821 tttgccaagt gcttaggtaa atggccgggc tcctcttacc tctgacttct ggagcgtgtc
     5881 cattctctgg ttgactgaat gtggcatttt cataaatcta ggagcagcag ccctcccaac
     5941 tgcaatttag taagcaggta gataagcttg catctgtttg catcttcctg cactgagttg
     6001 ggagtgcaaa accccgtact tggcctggtg agtaactgcc aacaagtatt tatgggtcat
     6061 ttgctaggtg gctagcatac ggcacggggc taatgagacc tggggcctgc tctcatatag
     6121 cttactgctc agtttctata ccagagcatc cgttggtgct tcttagccta ggcatgggtg
     6181 acaggcatgt ctggggacca ctttgggaga caacctagtg taaccttatt gcaaaggact
     6241 aagagacaaa aatcctgggt tctttctcca tcacttgctc tgtagctgaa tgaacaccac
     6301 ctcgatttct tgagtttcag tgtcttgttc tgtctgatga aagggttgga tatgtagttg
     6361 gataagcggt acaaattgtt agggttccag gccatgcctg atacatctga gtttttgctg
     6421 gaggtaggaa atttcaactc agaagctcat ccacagaaaa cagtgaccag cagcaggtat
     6481 ggagccataa ggctggaagg cttcaaagac cccttcttct gttactgtta tccacggcag
     6541 ttattctgtg taggtgtgaa ggagtggcta ctgactctgt gtggatcagc gtttacatat
     6601 gacctgatgg gagtgcatct ggtatgtgtt cctcccccct gtattgctgg agagattggc
     6661 aatcgtaggt atacacggaa gtgtcgggat gttcagtggc gcgcacttgg atgtttcttt
     6721 tcactttatt tctatagaaa caggttccag gagccagaga agtaggggaa aaaatccctg
     6781 agctcatctc tctctactac ttaataatgc aaacatttaa attcttggga agattttccc
     6841 gccccactcc ctacccccag tcacaggtca gaataggatt tccttcttag agaaggtagg
     6901 tcatggggcc tgcgattaaa aaaacaaaaa cataaaacaa acaaacaaac aaggtggctt
     6961 tccttggtct ttgtgattcc agctcttcac cagtgaagga attctttagt tccatagtgc
     7021 agcctgggtg ggtggagaag aggctgttat agtgggagcc agtgagcaaa gagggttgga
     7081 ggaggcagag gacagggctc agggaaggca agcctgtggc tctgggcatt ggccctcttg
     7141 tcaaaggtgt cagagcatgg cttcctgtga ggggctttcc cttgctcctg tgtgagaatc
     7201 tgtgcgttgt cttactggat gggggacctg gatggagatg ttaggggaga tagcttagtc
     7261 ttgttctaaa ctctagactt gctaaaaagg gttctcttga gtccaaatgc agtgccagag
     7321 ggtgagaagc agaattggcc agtagtgaac agtggtattg tgggtgataa gccctttctg
     7381 gtggccttaa atagcagtgc tagtccttga gtgccacagg gtcccctacc tccgtcctgg
     7441 cagtgctgat gggtttgttt cagcatattt tggttctcat tctcatttag ggaaaagggc
     7501 tgttgatgaa gggattatcc tcccaggcac caggggcctc agcacagttc cctgtgcttt
     7561 taaacactgt tgactttcat tagttattct aaagggattt atattggaga tacaactccc
     7621 tttggtttca tattatgaaa tagctttgta ctgtggaatg ctaactctgg aaagtctccc
     7681 tcataccaga accaaacatt catttctttt atatatatat atatattatc tccctggtac
     7741 tctccaggga ggtggttatt atcaagctga cccaaaggag tgcagtatgt gttctagttc
     7801 cttttctggt accttgttca acattctttt ttcctgaaga acatcctttt gtttatgaaa
     7861 acgtgaatag catcatgtaa aacaatccct tgctatcaga atgctccagt ttggccttaa
     7921 ttttgtaaac cttggagagg agtggagcgc ggctgtgaga agagatgtac catgtactct
     7981 ttctgtatca ttaaagtgca gtttttatgc ttct
//
