LOCUS       NM_030206               2079 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus cytoglobin (Cygb), transcript variant 1, mRNA.
ACCESSION   NM_030206 XM_992680
VERSION     NM_030206.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Porto,E., De Backer,J., Thuy,L.T.T., Kawada,N. and Hankeln,T.
  TITLE     Transcriptomics of a cytoglobin knockout mouse: Insights from
            hepatic stellate cells and brain
  JOURNAL   J Inorg Biochem 250, 112405 (2024)
   PUBMED   37977965
  REMARK    GeneRIF: Transcriptomics of a cytoglobin knockout mouse: Insights
            from hepatic stellate cells and brain.
REFERENCE   3  (bases 1 to 2079)
  AUTHORS   Rochon,E.R., Xue,J., Mohammed,M.S., Smith,C., Hay-Schmidt,A.,
            DeMartino,A.W., Clark,A., Xu,Q., Lo,C.W., Tsang,M., Tejero,J.,
            Gladwin,M.T. and Corti,P.
  TITLE     Cytoglobin regulates NO-dependent cilia motility and organ
            laterality during development
  JOURNAL   Nat Commun 14 (1), 8333 (2023)
   PUBMED   38097556
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2079)
  AUTHORS   Mathai,C., Jourd'heuil,F., Pham,L.G.C., Gilliard,K., Howard,D.,
            Balnis,J., Jaitovich,A., Chittur,S.V., Rilley,M., Peredo-Wende,R.,
            Ammoura,I., Shin,S.J., Barroso,M., Barra,J., Shishkova,E.,
            Coon,J.J., Lopez-Soler,R.I. and Jourd'heuil,D.
  TITLE     Regulation of DNA damage and transcriptional output in the
            vasculature through a cytoglobin-HMGB2 axis
  JOURNAL   Redox Biol 65, 102838 (2023)
   PUBMED   37573836
  REMARK    GeneRIF: Regulation of DNA damage and transcriptional output in the
            vasculature through a cytoglobin-HMGB2 axis.
REFERENCE   5  (bases 1 to 2079)
  AUTHORS   Okina,Y., Sato-Matsubara,M., Kido,Y., Urushima,H., Daikoku,A.,
            Kadono,C., Nakagama,Y., Nitahara,Y., Hoang,T.H., Thuy,L.T.T.,
            Matsubara,T., Ohtani,N., Ikeda,K., Yoshizato,K. and Kawada,N.
  TITLE     Nitric Oxide Derived from Cytoglobin-Deficient Hepatic Stellate
            Cells Causes Suppression of Cytochrome c Oxidase Activity in
            Hepatocytes
  JOURNAL   Antioxid Redox Signal 38 (7-9), 463-479 (2023)
   PUBMED   36112670
  REMARK    GeneRIF: Nitric Oxide Derived from Cytoglobin-Deficient Hepatic
            Stellate Cells Causes Suppression of Cytochrome c Oxidase Activity
            in Hepatocytes.
REFERENCE   6  (bases 1 to 2079)
  AUTHORS   Schmidt,M., Gerlach,F., Avivi,A., Laufs,T., Wystub,S.,
            Simpson,J.C., Nevo,E., Saaler-Reinhardt,S., Reuss,S., Hankeln,T.
            and Burmester,T.
  TITLE     Cytoglobin is a respiratory protein in connective tissue and
            neurons, which is up-regulated by hypoxia
  JOURNAL   J Biol Chem 279 (9), 8063-8069 (2004)
   PUBMED   14660570
  REMARK    GeneRIF: differential expression of cytoglobin argues against a
            general respiratory function of this molecule, but rather indicates
            a connective tissue-specific function
REFERENCE   7  (bases 1 to 2079)
  AUTHORS   Geuens,E., Brouns,I., Flamez,D., Dewilde,S., Timmermans,J.P. and
            Moens,L.
  TITLE     A globin in the nucleus!
  JOURNAL   J Biol Chem 278 (33), 30417-30420 (2003)
   PUBMED   12796507
  REMARK    GeneRIF: The nuclear localization of cytoglobin opens new
            perspectives for possible function(s) of globin-folded proteins as
            transcriptional regulators.
REFERENCE   8  (bases 1 to 2079)
  AUTHORS   Trent,J.T. 3rd and Hargrove,M.S.
  TITLE     A ubiquitously expressed human hexacoordinate hemoglobin
  JOURNAL   J Biol Chem 277 (22), 19538-19545 (2002)
   PUBMED   11893755
REFERENCE   9  (bases 1 to 2079)
  AUTHORS   Burmester,T., Ebner,B., Weich,B. and Hankeln,T.
  TITLE     Cytoglobin: a novel globin type ubiquitously expressed in
            vertebrate tissues
  JOURNAL   Mol Biol Evol 19 (4), 416-421 (2002)
   PUBMED   11919282
REFERENCE   10 (bases 1 to 2079)
  AUTHORS   Kawada,N., Kristensen,D.B., Asahina,K., Nakatani,K., Minamiyama,Y.,
            Seki,S. and Yoshizato,K.
  TITLE     Characterization of a stellate cell activation-associated protein
            (STAP) with peroxidase activity found in rat hepatic stellate cells
  JOURNAL   J Biol Chem 276 (27), 25318-25323 (2001)
   PUBMED   11320098
  REMARK    Erratum:[J Biol Chem 2001 Dec 14;276(50):47744-5]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL607039.25.
            
            On Mar 15, 2023 this sequence version replaced NM_030206.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK172475.1, AK087408.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-285               AL607039.25        81350-81634         c
            286-517             AL607039.25        77357-77588         c
            518-681             AL607039.25        76856-77019         c
            682-2079            AL607039.25        73168-74565         c
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 81.45 cM"
     gene            1..2079
                     /gene="Cygb"
                     /gene_synonym="3110001K20Rik; HGb; Staap"
                     /note="cytoglobin"
                     /db_xref="GeneID:114886"
                     /db_xref="MGI:MGI:2149481"
     exon            1..285
                     /gene="Cygb"
                     /gene_synonym="3110001K20Rik; HGb; Staap"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..715
                     /gene="Cygb"
                     /gene_synonym="3110001K20Rik; HGb; Staap"
                     /EC_number="1.15.1.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     stellate cell activation associated protein; histoglobin;
                     pseudoperoxidase CYGB; nitrite reductase CYGB; nitric
                     oxygen dioxygenase; superoxide dismutase CYGB"
                     /codon_start=1
                     /product="cytoglobin isoform 1"
                     /protein_id="NP_084482.1"
                     /db_xref="CCDS:CCDS25673.1"
                     /db_xref="GeneID:114886"
                     /db_xref="MGI:MGI:2149481"
                     /translation="MEKVPGDMEIERRERSEELSEAERKAVQATWARLYANCEDVGVA
                     ILVRFFVNFPSAKQYFSQFRHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKV
                     SSVLALVGKAHALKHKVEPMYFKILSGVILEVIAEEFANDFPVETQKAWAKLRGLIYS
                     HVTAAYKEVGWVQQVPNTTTPPATLPSSGP"
     exon            286..517
                     /gene="Cygb"
                     /gene_synonym="3110001K20Rik; HGb; Staap"
                     /inference="alignment:Splign:2.1.0"
     exon            518..681
                     /gene="Cygb"
                     /gene_synonym="3110001K20Rik; HGb; Staap"
                     /inference="alignment:Splign:2.1.0"
     exon            682..2079
                     /gene="Cygb"
                     /gene_synonym="3110001K20Rik; HGb; Staap"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2054..2059
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cygb"
                     /gene_synonym="3110001K20Rik; HGb; Staap"
                     /note="hexamer: ATTAAA"
     polyA_site      2079
                     /gene="Cygb"
                     /gene_synonym="3110001K20Rik; HGb; Staap"
                     /note="major polyA site"
ORIGIN      
        1 acacccggcg cgcacagaca cacgctccct ccctccggcg ctctactcct cgcccgcccg
       61 ccctcctgcc cgcctcgcac gcaccggcct cggctccccg cgcccgccgc cgagcgaagc
      121 tgcgctgggc tcggagctgc tcatggagaa agtgccgggc gacatggaga tagagcgtag
      181 ggagaggagc gaggagctgt ccgaggcgga gaggaaggcg gttcaggcta cgtgggcccg
      241 gctgtatgcc aactgcgagg acgtgggggt ggccatcctg gtgaggttct ttgtgaactt
      301 cccttcggcc aagcagtact tcagccagtt tagacacatg gaggatccct tggagatgga
      361 gaggagtccc cagctgcgga agcacgcctg ccgggtcatg ggggccctca acactgtcgt
      421 ggagaacctg catgacccag acaaggtatc ctctgtgctc gccctggtcg gcaaggccca
      481 cgccctcaag cacaaggtgg aacctatgta ctttaagatt ctctctgggg tcattctgga
      541 ggtgatcgcc gaggaatttg ccaatgactt ccctgtggag acgcagaaag cctgggccaa
      601 gctgcggggt ctcatctaca gccacgtgac cgcagcctac aaggaagtgg gctgggtaca
      661 gcaggtcccc aacaccacca ccccaccagc cactctgccc tcttcagggc cgtaagaccc
      721 ctcccagctc ccccctccct ggcagcactt tgggcagaag gccgacttct gaagacacgc
      781 attgaccttc catttctggg tgccaaggaa gctggaggaa cccctgactc gacttcccga
      841 aaggaggcct tcatcatggc tgtggtcccc ccaggctact gggaggtggg gctggatccc
      901 tggatgctga atctagcaga ggggaatccc tctgcctcac agcaggggca cactcttctt
      961 agctttttct actgactgtt agagagggac ctggcaaagt ggccggtggg aagcgaggac
     1021 aagcccgtga gtcacttcag aaaggaacta tccctgcttc actctgcccg ccagcatgca
     1081 ggttctcctc gccatctcta gagcatcacg cacacacatc taccatatac acagatatat
     1141 tctatatact atctatatat aaatatatat aacacacacg tacatatcta tcgagtgcgc
     1201 ctgcaggccc agaagcctag gtcttgccca gccgtgggag gccagaagag tcctggccat
     1261 ctttctgggc agctctaccg ggagcagggt gaaagctggc aacagcacaa gaggaggggc
     1321 caggtgggag agttggggtg agtcagcttg ctcctacagc tcagtgccct gcattcctgc
     1381 cctaccccca caaaacaaga gaatcccaga gcttgggagg cggaggaagg acaatcgttg
     1441 cgtctgaggt caggtggtca tctacctggt ctccacggtg gtgagtttca ggctagctgg
     1501 ggctacacag gaagacctcg ggtcaaacgg aagaaaaaaa aaaaacataa agatctggct
     1561 cccctctcct ctcctcccct cccctcccct ccccaagggt ttgcctgact ggcttgtcct
     1621 ggaaagggca aggatcaggg cacacaccaa gagttcagga gatcccaatg ggatgttggc
     1681 cagtgtatgt gtccggtata tgggggtggg ggtgaggggc agcctcagtg tggagcccta
     1741 gaatgtcagc ctcacctgct cacttcagac agcagccagg ccacaggacg ggggctgtcc
     1801 tcacacccct cagcagacca gggcctctct ctcctgccct cctctggggc cacctgctca
     1861 ccctctccac atagtctcct taacattgct gggaaccccg tgtgtacccc ttggtcacgt
     1921 gtactgtcct ggaagatgtc ttactgctgt ggctaccgtg cctgcgtccc ctctggtccc
     1981 accctcgcac agccttcctg taactgcctg tcttttgtag tttctgatgt ttgtaaccag
     2041 atccagctgt gtcattaaac caggtctgtt cttcctgtc
//
