LOCUS       NM_009989                666 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus cytochrome c, testis (Cyct), mRNA.
ACCESSION   NM_009989
VERSION     NM_009989.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Pinto,M., Vempati,U.D., Diaz,F., Peralta,S. and Moraes,C.T.
  TITLE     Ablation of Cytochrome c in Adult Forebrain Neurons Impairs
            Oxidative Phosphorylation Without Detectable Apoptosis
  JOURNAL   Mol Neurobiol 56 (5), 3722-3735 (2019)
   PUBMED   30191381
REFERENCE   2  (bases 1 to 666)
  AUTHORS   Auclair,G., Borgel,J., Sanz,L.A., Vallet,J., Guibert,S., Dumas,M.,
            Cavelier,P., Girardot,M., Forne,T., Feil,R. and Weber,M.
  TITLE     EHMT2 directs DNA methylation for efficient gene silencing in mouse
            embryos
  JOURNAL   Genome Res 26 (2), 192-202 (2016)
   PUBMED   26576615
REFERENCE   3  (bases 1 to 666)
  AUTHORS   Vempati,U.D., Han,X. and Moraes,C.T.
  TITLE     Lack of cytochrome c in mouse fibroblasts disrupts
            assembly/stability of respiratory complexes I and IV
  JOURNAL   J Biol Chem 284 (7), 4383-4391 (2009)
   PUBMED   19075019
REFERENCE   4  (bases 1 to 666)
  AUTHORS   Vempati,U.D., Diaz,F., Barrientos,A., Narisawa,S., Mian,A.M.,
            Millan,J.L., Boise,L.H. and Moraes,C.T.
  TITLE     Role of cytochrome C in apoptosis: increased sensitivity to tumor
            necrosis factor alpha is associated with respiratory defects but
            not with lack of cytochrome C release
  JOURNAL   Mol Cell Biol 27 (5), 1771-1783 (2007)
   PUBMED   17210651
REFERENCE   5  (bases 1 to 666)
  AUTHORS   Yiu,G.K., Gu,W. and Hecht,N.B.
  TITLE     Heterogeneity in the 5' untranslated region of mouse cytochrome cT
            mRNAs leads to altered translational status of the mRNAs
  JOURNAL   Nucleic Acids Res 22 (22), 4599-4606 (1994)
   PUBMED   7984407
REFERENCE   6  (bases 1 to 666)
  AUTHORS   Hake,L.E., Kuemmerle,N., Hecht,N.B. and Kozak,C.A.
  TITLE     The genes encoding the somatic and testis-specific isotypes of the
            mouse cytochrome c genes map to paralogous regions of chromosomes 6
            and 2
  JOURNAL   Genomics 20 (3), 503-505 (1994)
   PUBMED   8034327
REFERENCE   7  (bases 1 to 666)
  AUTHORS   Kelner,G.S., Minnerath,J.M., Jenkins,M.K. and Jemmerson,R.
  TITLE     The murine immune response to the male-specific antigen mouse
            testicular cytochrome c
  JOURNAL   Eur J Immunol 23 (8), 1992-1998 (1993)
   PUBMED   8393801
REFERENCE   8  (bases 1 to 666)
  AUTHORS   Hake,L.E., Alcivar,A.A. and Hecht,N.B.
  TITLE     Changes in mRNA length accompany translational regulation of the
            somatic and testis-specific cytochrome c genes during
            spermatogenesis in the mouse
  JOURNAL   Development 110 (1), 249-257 (1990)
   PUBMED   1964409
REFERENCE   9  (bases 1 to 666)
  AUTHORS   Virbasius,J.V. and Scarpulla,R.C.
  TITLE     Structure and expression of rodent genes encoding the
            testis-specific cytochrome c. Differences in gene structure and
            evolution between somatic and testicular variants
  JOURNAL   J Biol Chem 263 (14), 6791-6796 (1988)
   PUBMED   2834389
REFERENCE   10 (bases 1 to 666)
  AUTHORS   Hennig,B.
  TITLE     Change of cytochrome c structure during development of the mouse
  JOURNAL   Eur J Biochem 55 (1), 167-183 (1975)
   PUBMED   240690
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL845373.12 and BC049608.1.
            
            On Jul 31, 2014 this sequence version replaced NM_009989.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC049608.1, BY706711.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164133
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on expression, longest
                                                  protein
            regulatory uORF                    :: PMID: 7984407
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AL845373.12        141766-141811       c
            47-666              BC049608.1         1-620
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 44.94 cM"
     gene            1..666
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /note="cytochrome c, testis"
                     /db_xref="GeneID:13067"
                     /db_xref="MGI:MGI:88579"
     exon            1..141
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8..136
                     /regulatory_class="uORF"
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:7984407]"
                     /note="this uORF is predicted to encode a 42 aa peptide
                     (PMID:7984407)"
     exon            142..170
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /inference="alignment:Splign:2.1.0"
     exon            171..363
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..512
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /codon_start=1
                     /product="cytochrome c, testis-specific"
                     /protein_id="NP_034119.1"
                     /db_xref="CCDS:CCDS16156.1"
                     /db_xref="GeneID:13067"
                     /db_xref="MGI:MGI:88579"
                     /translation="MGDAEAGKKIFVQKCAQCHTVEKGGKHKTGPNLWGLFGRKTGQA
                     PGFSYTDANKNKGVIWSEETLMEYLENPKKYIPGTKMIFAGIKKKSEREDLIKYLKQA
                     TSS"
     exon            364..658
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      624..629
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /note="hexamer: AATAAA"
     polyA_site      658
                     /gene="Cyct"
                     /gene_synonym="T-Cc"
                     /note="major polyA site"
ORIGIN      
        1 agcgaggatg gagcccgcca tcagctggtg ctgggtcctg cctttaagag gagtccttgg
       61 gcacagcagt tgcaccttac atcttggcct ccctgcatca gtggaccaag ggtggcccag
      121 ggcacggctg ctgtgattgt gaatacaaag tataaggaaa caaagtccag tttaaggtct
      181 tattgcagag caaaatggga gatgctgaag caggcaagaa gatctttgtt cagaaatgtg
      241 ctcagtgcca cacggtggaa aaaggcggga aacacaagac aggtccaaat ctctggggcc
      301 tctttggtcg aaagacagga caagcaccag gattttctta cacggatgca aacaagaaca
      361 aaggtgttat ctggtcagaa gaaactttga tggaatattt ggagaacccg aagaaatata
      421 tccctggcac taaaatgatc tttgctggta tcaaaaagaa gagtgagaga gaagatctca
      481 ttaagtattt aaaacaggcc acatcctcat gaagtgaagt agtatttcag cttcaaaata
      541 ttcgctacca gtttcaaata ttgtgtttaa taagaagcat atctgatctt tttgatcagc
      601 ctgaagtaat ttaaacttgt taaaataaag gttgatattt ttgattgttt aaagtccaaa
      661 aaaaaa
//
