LOCUS       NM_028593               5208 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus cytochrome b reductase 1 (Cybrd1), mRNA.
ACCESSION   NM_028593 XM_130253 XM_910566 XM_993258
VERSION     NM_028593.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 5208)
  AUTHORS   Arsenault,P.R., Song,D., Chung,Y.J., Khurana,T.S. and Lee,F.S.
  TITLE     The Zinc Finger of Prolyl Hydroxylase Domain Protein 2 Is Essential
            for Efficient Hydroxylation of Hypoxia-Inducible Factor alpha
  JOURNAL   Mol Cell Biol 36 (18), 2328-2343 (2016)
   PUBMED   27325674
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5208)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 5208)
  AUTHORS   Luo,X., Hill,M., Johnson,A. and Latunde-Dada,G.O.
  TITLE     Modulation of Dcytb (Cybrd 1) expression and function by iron,
            dehydroascorbate and Hif-2alpha in cultured cells
  JOURNAL   Biochim Biophys Acta 1840 (1), 106-112 (2014)
   PUBMED   23981688
REFERENCE   6  (bases 1 to 5208)
  AUTHORS   Takeuchi,K., Bjarnason,I., Laftah,A.H., Latunde-Dada,G.O.,
            Simpson,R.J. and McKie,A.T.
  TITLE     Expression of iron absorption genes in mouse large intestine
  JOURNAL   Scand J Gastroenterol 40 (2), 169-177 (2005)
   PUBMED   15764147
  REMARK    GeneRIF: Iron absorption genes(Dcytb,IREG1,DMT1)were expressed at
            lower levels in mouse caecum and colon than in the duodenum. They
            are regulated by body iron requirements.
REFERENCE   7  (bases 1 to 5208)
  AUTHORS   Latunde-Dada,G.O., Vulpe,C.D., Anderson,G.J., Simpson,R.J. and
            McKie,A.T.
  TITLE     Tissue-specific changes in iron metabolism genes in mice following
            phenylhydrazine-induced haemolysis
  JOURNAL   Biochim Biophys Acta 1690 (2), 169-176 (2004)
   PUBMED   15469906
  REMARK    GeneRIF: Dcytb, DMT1, Ireg1 and transferrin receptor 1 have roles
            in iron transport and hemolysis
            Erratum:[Biochim Biophys Acta. 2005 Apr 15;1740(1):91]
REFERENCE   8  (bases 1 to 5208)
  AUTHORS   Vargas,J.D., Herpers,B., McKie,A.T., Gledhill,S., McDonnell,J., van
            den Heuvel,M., Davies,K.E. and Ponting,C.P.
  TITLE     Stromal cell-derived receptor 2 and cytochrome b561 are functional
            ferric reductases
  JOURNAL   Biochim Biophys Acta 1651 (1-2), 116-123 (2003)
   PUBMED   14499595
REFERENCE   9  (bases 1 to 5208)
  AUTHORS   Muckenthaler,M., Roy,C.N., Custodio,A.O., Minana,B., deGraaf,J.,
            Montross,L.K., Andrews,N.C. and Hentze,M.W.
  TITLE     Regulatory defects in liver and intestine implicate abnormal
            hepcidin and Cybrd1 expression in mouse hemochromatosis
  JOURNAL   Nat Genet 34 (1), 102-107 (2003)
   PUBMED   12704390
  REMARK    GeneRIF: Regulatory defects in liver and intestine implicate
            abnormal hepcidin and Cybrd1 expression in hemochromatosis.
REFERENCE   10 (bases 1 to 5208)
  AUTHORS   McKie,A.T., Barrow,D., Latunde-Dada,G.O., Rolfs,A., Sager,G.,
            Mudaly,E., Mudaly,M., Richardson,C., Barlow,D., Bomford,A.,
            Peters,T.J., Raja,K.B., Shirali,S., Hediger,M.A., Farzaneh,F. and
            Simpson,R.J.
  TITLE     An iron-regulated ferric reductase associated with the absorption
            of dietary iron
  JOURNAL   Science 291 (5509), 1755-1759 (2001)
   PUBMED   11230685
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK029112.1 and AL929228.5.
            
            On Feb 6, 2008 this sequence version replaced NM_028593.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029112.1, AK008849.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1590              AK029112.1         4-1593
            1591-5208           AL929228.5         183931-187548
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 42.38 cM"
     gene            1..5208
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /note="cytochrome b reductase 1"
                     /db_xref="GeneID:73649"
                     /db_xref="MGI:MGI:2654575"
     exon            1..259
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..939
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /EC_number="7.2.1.3"
                     /note="duodenal cytochrome b"
                     /codon_start=1
                     /product="plasma membrane ascorbate-dependent reductase
                     CYBRD1"
                     /protein_id="NP_082869.2"
                     /db_xref="CCDS:CCDS50603.1"
                     /db_xref="GeneID:73649"
                     /db_xref="MGI:MGI:2654575"
                     /translation="MAMEGYRGFLGLLVSALLVGFLSVIFVLIWVLHFREGLGWNGSG
                     LEFNWHPVLAVTGFVFIQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVV
                     AVFEYHNVQKVPHMYSLHSWVGLTALILYIQQLVVGFFVFLLPWAPPSLRAIVMPIHV
                     YSGLLLFGTVIATVLMGVTEKLFFVLKHPSYHSFPPEGVFTNTLGLLILVFGALIFWI
                     VTRPQWKRPREPGSVPLQLNGGNAECRMEGAIAISSAHSMDAADPADAESSSEGAARK
                     RTLGLADSGQRSTM"
     exon            260..468
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /inference="alignment:Splign:2.1.0"
     exon            469..623
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /inference="alignment:Splign:2.1.0"
     exon            624..5208
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1129
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /note="major polyA site"
     regulatory      3810..3815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /note="hexamer: AATAAA"
     polyA_site      3832
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
     regulatory      5190..5195
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /note="hexamer: AATAAA"
     polyA_site      5208
                     /gene="Cybrd1"
                     /gene_synonym="2210407P13Rik; Dcytb"
                     /note="major polyA site"
ORIGIN      
        1 agtaccccga ggccacggcc gcaacaccaa gttctccagc aggctgtcgt ggagcatggg
       61 gtgctgatgg ccatggaggg ctaccggggc ttcctggggc tgctggtgtc cgcgctgctc
      121 gtgggcttcc tgtcggtgat cttcgtgctc atctgggtcc tgcacttccg agaggggctt
      181 ggctggaacg gcagcgggct cgagtttaac tggcacccgg tgctcgcggt gaccggcttc
      241 gtcttcatcc agggcatcgc catcatcgtg tacagactgc catggacctg gaagtgcagc
      301 aagctcttga tgaaatccat ccatgccggg ttaaacgctg tggctgccat cctcgccatc
      361 atctccgtgg tggctgtgtt tgagtaccac aatgtccaaa aagtccccca catgtacagc
      421 ctgcacagct gggttggact gacagctctc atactctaca tccaacagct tgttgtaggc
      481 tttttcgtct ttctgcttcc atgggctccg ccctctctcc gggcaatcgt catgcccata
      541 cacgtgtatt ctggactcct cctcttcgga acagtgattg cgacggttct catgggagtg
      601 acagaaaagc tgttctttgt cctgaaacac ccctcgtacc attcattccc accagaaggt
      661 gtttttacaa atacgctggg ccttctgatt ctggtgtttg gagccctcat cttttggatt
      721 gtcaccaggc cccaatggaa acgccctaga gaaccaggct ctgtccctct ccagctaaat
      781 ggaggcaatg cagaatgcag aatggaaggc gccatagcaa taagctccgc ccacagtatg
      841 gacgcagctg acccagctga cgcagagtca agcagtgagg gagcagcccg gaagagaacc
      901 ctcggccttg ctgactctgg ccagaggtcc accatgtgaa atgtcgatac agccatgtgc
      961 attctcttcc aaagtcagcc ctacagttga gcttctccta cctagccata ggtggcttgg
     1021 gttcataggc caatgtataa tgcggtcacc aaggaggatt tcctgagagt aattgtgttg
     1081 actgaggcct gtgaacttac caaatgtaaa cggacatgat caacataaat agtgattgtt
     1141 tcttctttcc tcttgaggac cctggccatt gatgtagctg ccggtggctt gtctgatgaa
     1201 ctggtagcac ccttggcctc ttgctatgcc caattaattc tggaaggcca gtgatttcag
     1261 aactaggtag aacctctctc tctctctctc tctctctctc tctctctctc tctctctctc
     1321 tctctctctc tcttttggtg ttttgagaca gggtttctct gtatagccct ggctgtcctg
     1381 gaactcactt tgtagaccag gctggcctcg aactcagaaa tccgcctgcc tctgcctccc
     1441 gagtgctggg attaaaggcg tgcaccacca cgcccggcgg tagaacttct cttaactttt
     1501 aaaatatgat cacaaatact tggagcagga tttggaccat tgtgccacgt tgatgttgtg
     1561 agaaaaacat ggtggaggcg gggtgggtgc tttagccaag tatctagttc cctctcccgc
     1621 taatttgggc tcacaaaggc cctggctacg agagtaaata tgaggctgtt ttggagcagt
     1681 tgcttagtcg caggcagctg aagagatctc tggttagacc tgggagatgc ggtaacactc
     1741 agtccagggt ctgttactcc tcggagctga tgtacttcat ggctcatctt ctgaacctct
     1801 tgcaatgcaa acatgcccca gatcaagccc cgtaaaccgg tctcgcatcc acacactgag
     1861 aaaggtgccc tcgggtttct gtttttattt tccaaagtgc ttccctggct cttactctgt
     1921 gctttacgaa tattagctcc ttcagtctcc aggctgactc tgtgagacag gtattgttaa
     1981 ctctccccat ttttggaaag gttggtaact tggctcagca tcacacaact ctttaattga
     2041 tggaactgac attcaaaccc tggcagtctg gctcctctgc ccaagatcct atatttcacc
     2101 acactccttc cctatttatt ctaactggca gcactatggt ccacctatga atgaagttgc
     2161 agatgaaagc taatagaatt gttactggat gctaccttac ctttggtcct cttccaaggg
     2221 accttagatg atgggaaagg cgccacaaag atttggacgg agatcttggg gtctcagcca
     2281 ctgccaacaa gccagagcct gcctcgatgc ccttctaatc tttaacaggg catctgtctt
     2341 gctgacaaga agacctggac aaggatactc acagccttca agatgaaatc actaccgtat
     2401 tggttcctca ggatcttagc tgctgaacac atttttgttt ggagacacat atatcctggg
     2461 ctgtcttttc ctaggaccat aggcaggttc ctgccttatt tcagtggagt ttgaagttct
     2521 atgcagagag ctgaggttta gaagtgataa tctttcacat ggccagactt ctccctctgc
     2581 tccgatggaa acttgcagta ataactgtgc tgaaatgatg cctgtagcgg gtagtttggg
     2641 ctactccgct ttgagctcct agcaagtcgc aggctcgtac ctgcccagga gctctctcgg
     2701 atcctcagat gaaaggcaca cacacaatta gctcattttt aaattgcctt attggctctg
     2761 tggctgagca cgtctaagct tccatggcta gcgtgccctt acctttactc ctggctcaac
     2821 atctctaaag ccacccttaa cgtagttccc cagttgcctt ctgtgaagcc cattggtctt
     2881 gctgcccaag acgcttccaa gctgcccttc cgtgggctgc tttccctttc tgcccaaatt
     2941 ttggaagatc tcctctgcag ctctgagtct gttccatctt tctcccccaa gtaccttgat
     3001 tctccctttt ctgccttctc ttctctcaag cctgcaagaa tctggaagtc ccgcctcttt
     3061 ctttctgccc agccattggc cactggcatc tttattgata gatcaaaaac caattggagg
     3121 caaagacctc cagcttcaga aaacacagat tcctgattaa atctaagcat cagaaccact
     3181 cctctgcaga tgccttctgc agactggttg gtttttggtt tcggcgcagt ttctgattaa
     3241 gacatttggg caccagctgc ttgagaggta gctatctgag gcagagttca aggccagcct
     3301 gggcaacata gtgaaactct cttttgggat tttggtttct ctagtaaccc aagttgtcct
     3361 cactctttgt gtaattaagg gtgaccttgg acatgtggtt ctccttgtcc tcacttccca
     3421 aatgctgcgt tacaggcatg tgccaccagc caccatgtct tggcacagct ctgtctttta
     3481 aataaaaaat aaagccaaac caaacacatg aattttacag caaaatacac ttaatatgtt
     3541 tggtgtgtag tgaacttcct tttaggtaac atgattattt ccaaaagcca aatacaacct
     3601 tcaatctatc agatctccca aagtccataa cagctcagaa tcacttcaat gcatcatttc
     3661 ttgctttcca ttaattaatt ctgtttccaa aaccagtaac agcatcagaa gcactatgag
     3721 tgaggggccc aagatcattc ttcagctaga gagttcaaga ccagcctggg atacatgata
     3781 ccctatctat ctccgaagtc cgataaataa ataaagttca tatgcgtgct aagtaagagt
     3841 aattacgtgg ttacaccaaa cagtttggta aatgctgact gactataaag agctgggtca
     3901 aatcccaggg ttttggggga gtcatggggg tgaggagagg atggggggca gagtgggggg
     3961 agaatgagag gctagtaact gagccccaag agtcatggct gcctctcctt tccacgttgg
     4021 cctacagagg ctatcagtac aaacctaatt atgtggcact agaatagctc cattgctaag
     4081 tatttgagca ctcctctcag cccttccttt tctcccaagt gggctaatgg gagggtaata
     4141 ttataggtgt tccttttctt tcttttccag cttttatgaa aggatgcata gcacaatata
     4201 tcgaaaagat ctttctattt gagactcttc ttttaatggg ctctttattc taatgataat
     4261 tataccttta tctttcaaaa gctgatgttt ccttcctaaa gcaccccccc ttcccaaata
     4321 tcacattaaa aagtctgcaa agaatagggg agaagaaagg cttgggtatc aattccaaac
     4381 agtgattgaa acctcccaaa ataattatag cttatatctt tagcagagat tatctggtgt
     4441 ggcttacccc ataatctaat ttcaggaaag aaagctttat ttcaacaatg atctacgtca
     4501 acatgaaagc cttctctttc ccgggtctta gcaagaaatt gaaatgaata tactttatgg
     4561 aattaatgtc gtcaaaaagg tacagcttcc ctgtgctgtg tcaaacagag cccaccctcc
     4621 atggcacttt cctctcgtaa ggagtgtggt ttttgtttaa tctgtgcact tagaaacagc
     4681 aactgggacc tctccatgaa atgatagatt aaggcaggca tactttttaa ggactggtga
     4741 caagtagttt cttaaggatg gtgacaagta gtttctgttt tctgaaggac ggcgatgtag
     4801 attgcacgtc tcattttaat caggctttct tccctgccgg gctggaagac ccagagctca
     4861 cccactgacc gtgactgcac atggaaaacg gaaaggagga agccacaact tacatggact
     4921 tcagttgtga tgtgacgaga tgtttagcta taaatgtgca ctttttagat aactccaaca
     4981 ggaaactgta acttctatgt ctaataggga tgcagaaaag aaaaatgatt gttctttatt
     5041 tgggagctac ttaaacagat taagtgggaa ataaatctat ctataaattt gtctctttat
     5101 ctcagtacat tgatgaaaag tattatcttg actattttat agttttatag ggaagatgtg
     5161 catctatttt tcttgacttt taatatagca ataaaaaact acttctaa
//
