LOCUS       NM_144832               2483 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cytochrome b 245 chaperone 1 (Cybc1), transcript
            variant 1, mRNA.
ACCESSION   NM_144832
VERSION     NM_144832.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Randzavola,L.O., Mortimer,P.M., Garside,E., Dufficy,E.R.,
            Schejtman,A., Roumelioti,G., Yu,L., Pardo,M., Spirohn,K.,
            Tolley,C., Brandt,C., Harcourt,K., Nichols,E., Nahorski,M.,
            Woods,G., Williamson,J.C., Suresh,S., Sowerby,J.M., Matsumoto,M.,
            Santos,C.X.C., Kiar,C.S., Mukhopadhyay,S., Rae,W.M., Dougan,G.J.,
            Grainger,J., Lehner,P.J., Calderwood,M.A., Choudhary,J., Clare,S.,
            Speak,A., Santilli,G., Bateman,A., Smith,K.G.C., Magnani,F. and
            Thomas,D.C.
  TITLE     EROS is a selective chaperone regulating the phagocyte NADPH
            oxidase and purinergic signalling
  JOURNAL   Elife 11, e76387 (2022)
   PUBMED   36421765
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2483)
  AUTHORS   van der Weyden,L., Swiatkowska,A., Iyer,V., Speak,A.O. and
            Adams,D.J.
  TITLE     A Genome-Wide Screen in Mice To Identify Cell-Extrinsic Regulators
            of Pulmonary Metastatic Colonisation
  JOURNAL   G3 (Bethesda) 10 (6), 1869-1877 (2020)
   PUBMED   32245826
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2483)
  AUTHORS   Ryoden,Y., Fujii,T., Segawa,K. and Nagata,S.
  TITLE     Functional Expression of the P2X7 ATP Receptor Requires Eros
  JOURNAL   J Immunol 204 (3), 559-568 (2020)
   PUBMED   31862710
  REMARK    GeneRIF: these results indicated that Eros acts as a chaperone not
            only for NADPH oxidase, but also for P2X7, and contributes to the
            innate immune reaction
REFERENCE   5  (bases 1 to 2483)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2483)
  AUTHORS   Thomas,D.C., Charbonnier,L.M., Schejtman,A., Aldhekri,H.,
            Coomber,E.L., Dufficy,E.R., Beenken,A.E., Lee,J.C., Clare,S.,
            Speak,A.O., Thrasher,A.J., Santilli,G., Al-Mousa,H., Alkuraya,F.S.,
            Chatila,T.A. and Smith,K.G.C.
  TITLE     EROS/CYBC1 mutations: Decreased NADPH oxidase function and chronic
            granulomatous disease
  JOURNAL   J Allergy Clin Immunol 143 (2), 782-785 (2019)
   PUBMED   30312704
  REMARK    GeneRIF: this study shows that the function of EROS is fully
            conserved between human and mouse, and that homozygous mutations in
            EROS underlie a novel sixth cause of chronic granulomatous disease
REFERENCE   7  (bases 1 to 2483)
  AUTHORS   Thomas,D.C., Clare,S., Sowerby,J.M., Pardo,M., Juss,J.K.,
            Goulding,D.A., van der Weyden,L., Storisteanu,D., Prakash,A.,
            Espeli,M., Flint,S., Lee,J.C., Hoenderdos,K., Kane,L., Harcourt,K.,
            Mukhopadhyay,S., Umrania,Y., Antrobus,R., Nathan,J.A., Adams,D.J.,
            Bateman,A., Choudhary,J.S., Lyons,P.A., Condliffe,A.M.,
            Chilvers,E.R., Dougan,G. and Smith,K.G.
  TITLE     Eros is a novel transmembrane protein that controls the phagocyte
            respiratory burst and is essential for innate immunity
  JOURNAL   J Exp Med 214 (4), 1111-1128 (2017)
   PUBMED   28351984
REFERENCE   8  (bases 1 to 2483)
  AUTHORS   Liakath-Ali,K., Vancollie,V.E., Heath,E., Smedley,D.P., Estabel,J.,
            Sunter,D., Ditommaso,T., White,J.K., Ramirez-Solis,R., Smyth,I.,
            Steel,K.P. and Watt,F.M.
  TITLE     Novel skin phenotypes revealed by a genome-wide mouse reverse
            genetic screen
  JOURNAL   Nat Commun 5, 3540 (2014)
   PUBMED   24721909
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2483)
  AUTHORS   Sharov,A.A., Piao,Y., Matoba,R., Dudekula,D.B., Qian,Y.,
            VanBuren,V., Falco,G., Martin,P.R., Stagg,C.A., Bassey,U.C.,
            Wang,Y., Carter,M.G., Hamatani,T., Aiba,K., Akutsu,H., Sharova,L.,
            Tanaka,T.S., Kimber,W.L., Yoshikawa,T., Jaradat,S.A., Pantano,S.,
            Nagaraja,R., Boheler,K.R., Taub,D., Hodes,R.J., Longo,D.L.,
            Schlessinger,D., Keller,J., Klotz,E., Kelsoe,G., Umezawa,A.,
            Vescovi,A.L., Rossant,J., Kunath,T., Hogan,B.L., Curci,A.,
            D'Urso,M., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Transcriptome analysis of mouse stem cells and early embryos
  JOURNAL   PLoS Biol 1 (3), E74 (2003)
   PUBMED   14691545
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY202710.1 and BC017643.1.
            
            On Nov 18, 2011 this sequence version replaced NM_144832.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform 1. Variants 1-3 encode the same
            isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC017643.1, AK145884.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                BY202710.1         1-44
            45-2483             BC017643.1         1-2439
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 85.1 cM"
     gene            1..2483
                     /gene="Cybc1"
                     /note="cytochrome b 245 chaperone 1"
                     /db_xref="GeneID:217370"
                     /db_xref="MGI:MGI:2384959"
     exon            1..271
                     /gene="Cybc1"
                     /inference="alignment:Splign:2.1.0"
     exon            272..414
                     /gene="Cybc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             330..893
                     /gene="Cybc1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     uncharacterized protein C17orf62 homolog; eros; essential
                     for reactive oxygen species protein"
                     /codon_start=1
                     /product="cytochrome b-245 chaperone 1 isoform 1"
                     /protein_id="NP_659081.1"
                     /db_xref="CCDS:CCDS25771.1"
                     /db_xref="GeneID:217370"
                     /db_xref="MGI:MGI:2384959"
                     /translation="MYMQVETRTSTRLHLKRAPGIRSWSLLVGILSTGLAAAYYSGDS
                     LGWKLFYVTGCLFVAVQNLEDWEEAIFNKNTGKVILKTFSLYKKLLTLLRAGHDQVVV
                     LLKDIQDVNVEEEKVRYFGKGYMVVLRFATGFSHPLTQSAVMGRRSDVEAIAKLITSF
                     LELHRLESPSERSQSSDSEPDGPGGQS"
     exon            415..456
                     /gene="Cybc1"
                     /inference="alignment:Splign:2.1.0"
     exon            457..530
                     /gene="Cybc1"
                     /inference="alignment:Splign:2.1.0"
     exon            531..627
                     /gene="Cybc1"
                     /inference="alignment:Splign:2.1.0"
     exon            628..772
                     /gene="Cybc1"
                     /inference="alignment:Splign:2.1.0"
     exon            773..2417
                     /gene="Cybc1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2391..2396
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cybc1"
                     /note="hexamer: AATAAA"
     polyA_site      2417
                     /gene="Cybc1"
                     /note="major polyA site"
ORIGIN      
        1 agctcttatc ccggaagtct gcggaacaga agtccggaag ttcgaggggc tgttctctcc
       61 cactcattct cctacaagtc cctcgttgtc cgcataggtg cgtgtcctac aacttttcgg
      121 gttccgaccc gccgagccct gtgcgggtca tcgcttgcgg atctggacca ggcgcaaggc
      181 aaattgagga agccgggcgt gtgcactccg gaggaggagc cagcgtaacg ggccgctagt
      241 ggagcttgtt ggtaactgaa gccagaggga ggcttcagtt gtgtttttcc aagcctggtc
      301 ggctgtgcag tagagcccag gttccaagaa tgtatatgca ggtggagaca cgcaccagca
      361 cccgcctcca tctgaagagg gctccaggca tccggtcttg gtccctgctg gtcggaatat
      421 tatccactgg cctggctgct gcctattaca gtggagatag tctgggctgg aagctcttct
      481 atgtcacagg ctgcctattt gtggctgtac agaacttgga agactgggag gaggccattt
      541 tcaacaagaa cactgggaag gtcatcttaa aaacattcag cctctacaag aagctgctga
      601 ctcttctcag agcgggccac gatcaagtgg tggttctgtt gaaagacatc caggatgtga
      661 atgtggagga ggaaaaggtc cgttactttg ggaagggcta catggtggtg ctccggtttg
      721 caacaggctt ctcccaccct ctcactcaga gtgcagtcat gggccgacgc agtgatgtgg
      781 aagccattgc caaactcatc actagcttct tggagctgca ccgcctggag agcccctcag
      841 aacggtcaca gagcagcgac agtgaacctg atggccctgg tggccagagt tgacagtgtg
      901 gactggctgc aggtaaatgc catccacttc tgatgcatct ccaaccgact tgacaattgt
      961 aagggtacag gcagatcttg ggcacctgcc tgtggctcct tgggcagttc tactatgggc
     1021 cacctgcctt ttgcttgttt atgcttctac actttgcaga accttgggct agacctctgc
     1081 cacaacccag aaaggttgca tctatcagga tgcagggtgt ggatgctaca tctagggggg
     1141 gcaaggtctc agaaatgagt gccttctcca gagttacact gcagctctgg gttgggcagc
     1201 acagcctggt ctggataagc tcacccaagg gcaatctgaa gcagaaaatg ccaagctctc
     1261 ttcagcatag gcctagtgcc tgccttggag acaccttgcc actccaggct actgtaagca
     1321 agactccatt cctagtgagt gagacaccta tatgcacgtg ggcagacaca ctcctatgca
     1381 cagtgatgtc tgttccctct gaccatccct caagatggac actctcagtg gtcaggggct
     1441 tccctgaagg caactctgga tgctggacac ctgaccacag aactctcaga ggaatccgag
     1501 tgctgctgac tacccagttc cttcttagac catgtgtctg agagcccagc agtgttggag
     1561 cccagctgtc aggaatggaa ggctctccca acccagcctg gaaagaaggt gctagcagag
     1621 gaaagatcac ctctcctggc atcctgcagc ctttaagacc tgggggatcc cttacagaaa
     1681 cagtcttagc ctttcgagcc ttgaaaagac ctgaagaaaa caggcatgtg gagacacagc
     1741 ccaataaatc ccagtgttga gaactgcagc gggaagaacg ttcaaggcca gcctgggcca
     1801 tggcctcttc taagaaaagt tggtaccaac cactaatggt ccttttctgc tcttctagtc
     1861 tccttctggg tggactcaag ttgctcaaaa gtacattgtt tactaagttc ccagcctggc
     1921 ctctaggcct ggaagctgtg gctctctggc ctaacacagc tcctcgaagc ctcatacgca
     1981 tacctcatca ggcagaactg gggaattgaa gttacataga caaaggcctt gaggccactg
     2041 tacctaagcc gagcatggta gttaggcaag aactatgaac aggtcttctg aagtgactgc
     2101 cactagttct gacttgggga atatggtccc ttaaacatgc caaagtgagc tttttaagtg
     2161 gataagttag agatttctct ccccagatga aacgtgaagt atagcttgtt gggttttggc
     2221 ttgttgccta taggctctta aggtacttta gacaagagag gaaggtgtct taggtgctgt
     2281 catagactca ctcctgggtg acagcattca attctctatg gtggtaaaaa gctgattaga
     2341 gtttctgctg gtgggacttc cacttctact ggtcttttta tttcagaagt aataaagctc
     2401 tacatgcaac ttgcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2461 aaaaaaaaaa aaaaaaaaaa aaa
//
