LOCUS       NM_024195               2653 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus cytochrome b5 reductase 4 (Cyb5r4), transcript variant
            1, mRNA.
ACCESSION   NM_024195
VERSION     NM_024195.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Stroh,M.A., Winter,M.K., McCarson,K.E., Thyfault,J.P. and Zhu,H.
  TITLE     NCB5OR Deficiency in the Cerebellum and Midbrain Leads to
            Dehydration and Alterations in Thirst Response, Fasted Feeding
            Behavior, and Voluntary Exercise in Mice
  JOURNAL   Cerebellum 17 (2), 152-164 (2018)
   PUBMED   28887630
  REMARK    GeneRIF: the findings of this study suggest altered feeding
            response post starvation as a result of NCB5OR deficiency in the
            cerebellum.
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Stroh,M.A., Winter,M.K., Swerdlow,R.H., McCarson,K.E. and Zhu,H.
  TITLE     Loss of NCB5OR in the cerebellum disturbs iron pathways,
            potentiates behavioral abnormalities, and exacerbates
            harmaline-induced tremor in mice
  JOURNAL   Metab Brain Dis 31 (4), 951-964 (2016)
   PUBMED   27188291
  REMARK    GeneRIF: Our findings suggest an essential role for NCB5OR in
            maintaining both iron homeostasis and the proper functioning of
            various locomotor pathways in the mouse cerebellum and midbrain.
REFERENCE   3  (bases 1 to 2653)
  AUTHORS   Stiborova,M., Moserova,M., Cerna,V., Indra,R., Dracinsky,M.,
            Sulc,M., Henderson,C.J., Wolf,C.R., Schmeiser,H.H., Phillips,D.H.,
            Frei,E. and Arlt,V.M.
  TITLE     Cytochrome b5 and epoxide hydrolase contribute to
            benzo[a]pyrene-DNA adduct formation catalyzed by cytochrome P450
            1A1 under low NADPH:P450 oxidoreductase conditions
  JOURNAL   Toxicology 318, 1-12 (2014)
   PUBMED   24530354
  REMARK    GeneRIF: In livers of HRN mice Cyp1a1, cytochrome b5 and mEH can
            effectively activate BaP to DNA binding species, even in the
            presence of very low amounts of P450 oxidoreductase.
REFERENCE   4  (bases 1 to 2653)
  AUTHORS   Wang,W., Guo,Y., Xu,M., Huang,H.H., Novikova,L., Larade,K.,
            Jiang,Z.G., Thayer,T.C., Frontera,J.R., Aires,D., Ding,H., Turk,J.,
            Mathews,C.E., Bunn,H.F., Stehno-Bittel,L. and Zhu,H.
  TITLE     Development of diabetes in lean Ncb5or-null mice is associated with
            manifestations of endoplasmic reticulum and oxidative stress in
            beta cells
  JOURNAL   Biochim Biophys Acta 1812 (11), 1532-1541 (2011)
   PUBMED   21839170
REFERENCE   5  (bases 1 to 2653)
  AUTHORS   Xu,M., Wang,W., Frontera,J.R., Neely,M.C., Lu,J., Aires,D.,
            Hsu,F.F., Turk,J., Swerdlow,R.H., Carlson,S.E. and Zhu,H.
  TITLE     Ncb5or deficiency increases fatty acid catabolism and oxidative
            stress
  JOURNAL   J Biol Chem 286 (13), 11141-11154 (2011)
   PUBMED   21300801
  REMARK    GeneRIF: FFA accumulation and catabolism and oxidative stress are
            major consequences of Ncb5or deficiency in liver.
REFERENCE   6  (bases 1 to 2653)
  AUTHORS   Zhu,H., Larade,K., Jackson,T.A., Xie,J., Ladoux,A., Acker,H.,
            Berchner-Pfannschmidt,U., Fandrey,J., Cross,A.R.,
            Lukat-Rodgers,G.S., Rodgers,K.R. and Bunn,H.F.
  TITLE     NCB5OR is a novel soluble NAD(P)H reductase localized in the
            endoplasmic reticulum
  JOURNAL   J Biol Chem 279 (29), 30316-30325 (2004)
   PUBMED   15131110
  REMARK    GeneRIF: NCB5OR is a novel soluble NAD(P)H reductase localized in
            the endoplasmic reticulum
REFERENCE   7  (bases 1 to 2653)
  AUTHORS   Oikawa,K., Kimura,S., Aoki,N., Atsuta,Y., Takiyama,Y., Nagato,T.,
            Yanai,M., Kobayashi,H., Sato,K., Sasajima,T. and Tateno,M.
  TITLE     Neuronal calcium sensor protein visinin-like protein-3 interacts
            with microsomal cytochrome b5 in a Ca2+-dependent manner
  JOURNAL   J Biol Chem 279 (15), 15142-15152 (2004)
   PUBMED   14739275
REFERENCE   8  (bases 1 to 2653)
  AUTHORS   Curry,B.J., Roman,S.D., Wallace,C.A., Scott,R., Miriami,E. and
            Aitken,R.J.
  TITLE     Identification and characterization of a novel splice variant of
            mouse and rat cytochrome b5/cytochrome b5 reductase
  JOURNAL   Genomics 83 (3), 425-438 (2004)
   PUBMED   14962668
REFERENCE   9  (bases 1 to 2653)
  AUTHORS   Davis,C.A., Dhawan,I.K., Johnson,M.K. and Barber,M.J.
  TITLE     Heterologous expression of an endogenous rat cytochrome
            b(5)/cytochrome b(5) reductase fusion protein: identification of
            histidines 62 and 85 as the heme axial ligands
  JOURNAL   Arch Biochem Biophys 400 (1), 63-75 (2002)
   PUBMED   11913972
REFERENCE   10 (bases 1 to 2653)
  AUTHORS   Zhu,H., Qiu,H., Yoon,H.W., Huang,S. and Bunn,H.F.
  TITLE     Identification of a cytochrome b-type NAD(P)H oxidoreductase
            ubiquitously expressed in human cells
  JOURNAL   Proc Natl Acad Sci U S A 96 (26), 14742-14747 (1999)
   PUBMED   10611283
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK090159.1 and CO801035.1.
            
            On Jul 19, 2008 this sequence version replaced NM_024195.1.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus to one that is more supported by available conservation
            data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK090159.1, AK157312.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1753              AK090159.1         3-1755
            1754-2302           AK090159.1         1757-2305
            2303-2653           CO801035.1         223-573
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 46.69 cM"
     gene            1..2653
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /note="cytochrome b5 reductase 4"
                     /db_xref="GeneID:266690"
                     /db_xref="MGI:MGI:2386848"
     exon            1..225
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     CDS             151..1737
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /EC_number="1.6.2.2"
                     /note="isoform 1 is encoded by transcript variant 1; NADPH
                     cytochrome B5 oxidoreductase; cb5/cb5R; flavohemoprotein
                     b5/b5R; N-terminal cytochrome b5 and cytochrome b5
                     oxidoreductase domain-containing protein; cytochrome
                     b-type NAD(P)H oxidoreductase"
                     /codon_start=1
                     /product="cytochrome b5 reductase 4 isoform 1"
                     /protein_id="NP_077157.2"
                     /db_xref="CCDS:CCDS40716.2"
                     /db_xref="GeneID:266690"
                     /db_xref="MGI:MGI:2386848"
                     /translation="MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSG
                     KDLTGLKGGLIEVTEEELKKHNKKEDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGA
                     DGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCHEGKRVLNGMLPKSQMSDTLP
                     RDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDSLRAEAVIK
                     DHSYLVHVGLSHEVQENFSVRVIENVGKIEIVLQKKESVSWQCLGDHLEKHDSFIPKK
                     DTGLYYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYT
                     PVSDSLLSDFKEPVLSPNKYICFLIKIYPAGLFTPELDRLQIGDFISVSGPEGDFKVS
                     KLQEVEDLFLLAAGTGFTPMVTVLNYALSHMSSLRKVKLMFFNKTEDDIIWRCQLEKL
                     ALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSSENSRAFLCICGPTPFTDE
                     GIRLLHDLNFSDDEIHGFTA"
     exon            226..379
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            380..480
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            481..562
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            563..592
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            593..677
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            678..735
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            736..829
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            830..862
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            863..985
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1126
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1279
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1430
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1517
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1682
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..2629
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2210..2215
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /note="hexamer: AATAAA"
     polyA_site      2236
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /note="major polyA site"
     regulatory      2270..2275
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /note="hexamer: AATACA"
     polyA_site      2293
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
     regulatory      2598..2603
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
                     /note="hexamer: AATAAA"
     polyA_site      2629
                     /gene="Cyb5r4"
                     /gene_synonym="2810034J18Rik; B5+B5R; b5/b5r; b5b5r;
                     Cb5cb5r; Ncb5or"
ORIGIN      
        1 cgcctgaccc ctggcgaccc cggaagtggg tcgggggctt ggcctcggcc gggccaagga
       61 gcccgcacgg acggtggtgg gcgctggggt cccaccaggg cagcctgtcc ggtggcggag
      121 cccggatgcg gcccgagccg ggggcccagg atgctgaacg tcccttccca ggctttcccg
      181 gcccccggct cccagcagcg cgtctcctcc caggggcgca gcaaggtacc tttaaaacaa
      241 ggcaggagtc ttatggattg gatccgactg accaagagtg gaaaggactt aacaggattg
      301 aagggcgggt taattgaagt aactgaagaa gagcttaaga agcacaacaa gaaagaagat
      361 tgttggatat gcataagagg gtttgtttac aacgtcagcc cttatatgga gtaccatccg
      421 ggtggggagg atgaactcat gagagccgca ggagccgatg gaacagatct ttttaatgag
      481 gttcatcgtt gggtcaatta tgaatccatg ctgaaagaat gcctggttgg caggatggct
      541 gtgaagcctg ctgttccaaa agactgtcat gaaggaaaga gagtcttaaa tggcatgctt
      601 cccaagagcc aaatgtcaga cacactgccc agggatgtga cagacacact gcccagggaa
      661 gacctcagtt ctccaagcta tgattggttc caaacagagt cttcagtcac cattgttgta
      721 tacaccaaac aaaagaatat cagtttagac tcagtaatcg ttgacctcca ggatgactcc
      781 ttgagagcag aagcagtcat caaggatcac tcttaccttg tacatgttgg gctaagccat
      841 gaagttcaag aaaatttttc tgtacgggtt attgaaaatg ttggaaaaat agagattgtc
      901 ctacaaaaga aagaaagtgt ttcttggcaa tgtcttggtg atcacctgga gaaacatgat
      961 tctttcattc caaagaaaga tacaggcttg tactacagac ggtgccagtt aatatctaag
     1021 gaagatgtca ctcatgacac gagacttttc tgtctgatgc tgcctccaag tactcacctt
     1081 caggtgccag ttggacaaca tgtttacctc aagctcagtg tcacaggtgc agaaatagta
     1141 aagccatata cacccgtgtc tgattcctta ctctcagatt ttaaagaacc agtcctttcc
     1201 ccaaataaat acatctgctt tttgatcaaa atctatcctg ctggactctt cacaccagag
     1261 cttgatcgtc ttcagattgg agattttatt tctgtaagcg gacctgaagg cgattttaaa
     1321 gtatccaaac tccaggaagt agaagatctc tttctattgg cagccggaac aggattcaca
     1381 ccaatggtca ctgtactgaa ttatgctttg tctcacatgt ccagtctcag gaaggtgaag
     1441 ttgatgttct tcaataaaac agaggacgat ataatttgga gatgtcagct agagaagttg
     1501 gcattaagag aaaaaagatt tgatgttgaa tttgttctct cggcaccttc tccagaatgg
     1561 aatggcaaac agggacacat atcacgagct cttctctctg aatttttgca aagaagttca
     1621 gaaaactcta gggccttcct ttgcatctgc ggaccaacac catttactga cgaggggata
     1681 aggttgctgc atgatcttaa cttttctgat gatgagattc acggattcac cgcttaagaa
     1741 aaagctgcca ttgtctttta ttcaactagt ttatatttgt gattgcctag gagtttttaa
     1801 atgagttttt gtatgtatgg aaggctttgc tttctgaaat gaaatcatgt gtcttcagta
     1861 caagtaactt cttggtttaa ttttgtaccg tgacttatct tactgctgac cctgacctgg
     1921 agggtcagtc atggcagcag gtgcatatgg gagcctttgt tatgaactgc aagtttcctg
     1981 accactgaag ttgtatcact gttcggcagt caacacttgt attttatgac atgataaact
     2041 aagcaatcat tttggttact ttttatgctg caagatatct tctgagcaat atgtattaaa
     2101 tattccactg cactgttcac tcaggaattg atggtgtatg tccttagtag tcttaaaact
     2161 tggaatgctg taactattgg tggtccagtt ttgtgaactg gacagataga ataaaactat
     2221 acttccatac tttttactcc ctgtggtgga cgtgtggctg aaggtaacaa atacagtggt
     2281 tgtcttaaag ggagttttat ttacatactg ttttttgagt ctcacttaat agctttcgtt
     2341 gctgaaattg gctatgtata ccaggctggc ctaaaattcc tacttctgct tttgggggct
     2401 gggattacag gtatgtgcta ccattcttga gcttaaagga gtttttaaag acaccagcag
     2461 agttcaattt cccctcccca gagggtggaa gaagagaact gactgtgtaa gatattctca
     2521 cgtgcacact gtggcagtgc accagcccac aaacacggac agtaaagaaa tataatctct
     2581 agaatgttta cgataacaat aaaaatatta gaagttcttc acacttgcaa aaaaaaaaaa
     2641 aaaaaaaaaa aaa
//
