LOCUS       NM_029787               2028 bp    mRNA    linear   ROD 12-NOV-2024
DEFINITION  Mus musculus cytochrome b5 reductase 3 (Cyb5r3), mRNA.
ACCESSION   NM_029787
VERSION     NM_029787.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Sanchez-Mendoza,L.M., Perez-Sanchez,C., Garcia-Caballero,C.,
            Perez-Rodriguez,M., Calero-Rodriguez,P., Vellon-Garcia,B.,
            Moreno,J.A., Buron,M.I., de Cabo,R., Gonzalez-Reyes,J.A. and
            Villalba,J.M.
  TITLE     CYB5R3 overexpression exhibits sexual dimorphism: Mitochondrial and
            metabolic adaptations in transgenic female mice during calorie
            restriction
  JOURNAL   Free Radic Biol Med 223, 69-86 (2024)
   PUBMED   39069267
  REMARK    GeneRIF: CYB5R3 overexpression exhibits sexual dimorphism:
            Mitochondrial and metabolic adaptations in transgenic female mice
            during calorie restriction.
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Chen,J., Hu,J., Guo,X., Yang,Y., Qin,D., Tang,X., Huang,Z.,
            Wang,F., Hu,D., Peng,D. and Yu,B.
  TITLE     Apolipoprotein O modulates cholesterol metabolism via NRF2/CYB5R3
            independent of LDL receptor
  JOURNAL   Cell Death Dis 15 (6), 389 (2024)
   PUBMED   38830896
  REMARK    GeneRIF: Apolipoprotein O modulates cholesterol metabolism via
            NRF2/CYB5R3 independent of LDL receptor.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2028)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2028)
  AUTHORS   Watanabe,H., Asahara,S.I., Son,J., McKimpson,W.M., de Cabo,R. and
            Accili,D.
  TITLE     Cyb5r3 activation rescues secondary failure to sulfonylurea but not
            beta-cell dedifferentiation
  JOURNAL   PLoS One 19 (2), e0297555 (2024)
   PUBMED   38335173
  REMARK    GeneRIF: Cyb5r3 activation rescues secondary failure to
            sulfonylurea but not beta-cell dedifferentiation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2028)
  AUTHORS   Im,J.Y., Kim,S.J., Park,J.L., Han,T.H., Kim,W.I., Kim,I., Ko,B.,
            Chun,S.Y., Kang,M.J., Kim,B.K., Jeon,S.A., Kim,S.K., Ryu,I.,
            Kim,S.Y., Nam,K.H., Hwang,I., Ban,H.S. and Won,M.
  TITLE     CYB5R3 functions as a tumor suppressor by inducing ER
            stress-mediated apoptosis in lung cancer cells via the PERK-ATF4
            and IRE1alpha-JNK pathways
  JOURNAL   Exp Mol Med 56 (1), 235-249 (2024)
   PUBMED   38253797
  REMARK    GeneRIF: CYB5R3 functions as a tumor suppressor by inducing ER
            stress-mediated apoptosis in lung cancer cells via the PERK-ATF4
            and IRE1alpha-JNK pathways.
REFERENCE   6  (bases 1 to 2028)
  AUTHORS   Yoshida,T. and Tsuda,H.
  TITLE     Gene targeting of DT-diaphorase in mouse embryonic stem cells:
            establishment of null mutant and its mitomycin C-resistance
  JOURNAL   Biochem Biophys Res Commun 214 (2), 701-708 (1995)
   PUBMED   7677784
REFERENCE   7  (bases 1 to 2028)
  AUTHORS   Hawkes,R. and Turner,R.W.
  TITLE     Compartmentation of NADPH-diaphorase activity in the mouse
            cerebellar cortex
  JOURNAL   J Comp Neurol 346 (4), 499-516 (1994)
   PUBMED   7527060
REFERENCE   8  (bases 1 to 2028)
  AUTHORS   Bucan,M., Gatalica,B., Nolan,P., Chung,A., Leroux,A.,
            Grossman,M.H., Nadeau,J.H., Emanuel,B.S. and Budarf,M.
  TITLE     Comparative mapping of 9 human chromosome 22q loci in the
            laboratory mouse
  JOURNAL   Hum Mol Genet 2 (8), 1245-1252 (1993)
   PUBMED   8401507
REFERENCE   9  (bases 1 to 2028)
  AUTHORS   Francke,U., Tetri,P., Taggart,R.T. and Oliver,N.
  TITLE     Conserved autosomal syntenic group on mouse (MMU) chromosome 15 and
            human (HSA) chromosome 22: assignment of a gene for arylsulfatase A
            to MMU 15 and regional mapping of DIA1, ARSA, and ACO2 on HSA 22
  JOURNAL   Cytogenet Cell Genet 31 (2), 58-69 (1981)
   PUBMED   6118238
REFERENCE   10 (bases 1 to 2028)
  AUTHORS   Taggart,R.T., Tetri,P. and Francke,U.
  TITLE     Assignment of the gene for NADH diaphorase Dia-1 to Mouse
            chromosome 15
  JOURNAL   Somatic Cell Genet 6 (6), 769-776 (1980)
   PUBMED   6893763
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC043074.1 and AI839369.1.
            
            On Jul 12, 2018 this sequence version replaced NM_029787.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC004760.2, BC043074.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2006              BC043074.1         26-2031
            2007-2028           AI839369.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 39.4 cM"
     gene            1..2028
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /note="cytochrome b5 reductase 3"
                     /db_xref="GeneID:109754"
                     /db_xref="MGI:MGI:94893"
     exon            1..72
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..957
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /EC_number="1.6.2.2"
                     /note="diaphorase 1 (NADH)"
                     /codon_start=1
                     /product="NADH-cytochrome b5 reductase 3"
                     /protein_id="NP_084063.1"
                     /db_xref="CCDS:CCDS27698.1"
                     /db_xref="GeneID:109754"
                     /db_xref="MGI:MGI:94893"
                     /translation="MGAQLSTLSHVVLSPVWFIYSLFMKLFQRSTPAITLENPDIKYP
                     LRLIDKEVISPDTRRFRFALPSPQHILGLPIGQHIYLSTRIDGNLVIRPYTPVSSDDD
                     KGFVDLVVKVYFKDTHPKFPAGGKMSQYLENMKIGDTIEFRGPNGLLVYQGKGKFAIR
                     ADKKSNPVVRTVKSVGMIAGGTGITPMLQVIRAVLKDPNDHTVCYLLFANQSEKDILL
                     RPELEELRNEHSARFKLWYTVDKAPDAWDYSQGFVNEEMIRDHLPTPGEEPLILMCGP
                     PPMIQFACLPNLERVGHPKERCFTF"
     exon            73..204
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     exon            205..277
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     exon            278..384
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     exon            385..514
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     exon            515..598
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     exon            599..684
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     exon            685..784
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     exon            785..2012
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1991..1996
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /note="hexamer: AGTAAA"
     polyA_site      2012
                     /gene="Cyb5r3"
                     /gene_synonym="0610016L08Rik; 2500002N19Rik; B5R; Dia-1;
                     Dia1; WU:Cyb5r3"
                     /note="major polyA site"
ORIGIN      
        1 gagtagcggg tgtagaaccg tgcgtgaggc caccgtctct tcttcgccac catgggggcc
       61 cagctgagca cgttgagcca cgtggtgctc tcgcccgtct ggttcatcta cagtctcttc
      121 atgaagctgt ttcagcgctc cacaccggcc atcaccctcg agaaccccga catcaagtac
      181 cctctgaggc tcatcgacaa ggaggtcatc agtcctgaca ctcggcgctt ccgatttgcc
      241 ctcccttcac cccagcacat cctgggcctt cctattggcc aacacatcta cctctcgact
      301 aggatcgatg gcaacttggt cattcggccc tacacccctg tgtcgagcga tgatgacaag
      361 ggctttgtgg acttggtggt caaggtttac ttcaaggaca cccatcccaa gtttccagct
      421 ggagggaaaa tgtctcagta cctggaaaac atgaagattg gagacaccat tgaattccgg
      481 ggtcccaatg ggctactggt ctaccagggc aaagggaagt ttgccatccg tgcagacaag
      541 aagtccaacc ccgttgtcag gacggtgaag tctgtaggca tgattgcagg agggacaggc
      601 atcaccccaa tgctgcaggt gatccgagcc gtcctgaagg acccaaacga ccacaccgtg
      661 tgctatctgc tctttgccaa ccagtccgag aaagatatcc tgttgcggcc tgagctggag
      721 gaactgagga acgaacattc tgctcgcttc aagctctggt acacagtgga caaagcccct
      781 gatgcctggg actatagcca gggcttcgtg aatgaggaga tgatcaggga ccaccttcca
      841 actcctgggg aggagccgct gatactgatg tgtggacccc caccaatgat ccagtttgcc
      901 tgtttgccaa acctggagcg tgtgggccat cccaaggagc gatgcttcac cttctgatgg
      961 ctggacgctg gccaagcctg tgcctgctgc tcacgcactc accacaacca cctgtccacc
     1021 ccttccttcc ccaccactgt cgctcaccct gacatatgcc cacatccatg ctggggcctg
     1081 ggttcagcct ggcctgccca gccctggtca tccagctgta ctggcccctg gggggcccct
     1141 ttgggagcag gcctgtgtat caggtgactt ctgttgacca ctctctgaat aggcttctgt
     1201 ctggtactaa ctggccatta ccagagatgt cctgtgacta cccccttaca catagacaca
     1261 cacacacaca cacacacaca cacagagaca ggagagagag agagagagag agagagagag
     1321 gagagagagg agagagagag aggagagaga gacaggagag agagagagga gagagaagag
     1381 gaagagagag agagagagag ggagagagag gagagagaga ggagagagag agagaagagg
     1441 aagagagagg gagggagagc gtgagcgtga gctgtcagta ccatgtctac ccattgccat
     1501 gaactgtggt ctatagaaag gaagtgaaag gtaaatagtc acctacggtc ctaggaacca
     1561 tgcctgccac ctaaccacct acctgcctta ggccatagcc tggcctgtgc ccctaaacac
     1621 tacattagac agtgacaccc aaaggttttc ttaggagggt gtctggcaga gacaggccag
     1681 cccccacttg ctggccaggg gtcccttggg gcagcacgtg gacccttcca aaactataca
     1741 cactgccccc gactccatgc agtcttgagt gccctaagtt gtcagcccaa cagagtttgg
     1801 tagaccttgg gggtccctgg cttatctctc caccccaaac ctccttcctg tctggtcccc
     1861 agcctggggg ggttctgagc agtgcctctt gtcttggaga cagacctggt gcctggcgct
     1921 gccttcagca ggcagcaggc agcctcctgc acactggctt ttttagtcat ttatgggcaa
     1981 aaagagttaa agtaaaactt tgcaaatccc gaaaaaaaaa aaaaaaaa
//
