LOCUS       NM_177216               2724 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus cytochrome b5 reductase 2 (Cyb5r2), transcript variant
            2, mRNA.
ACCESSION   NM_177216
VERSION     NM_177216.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 2724)
  AUTHORS   Probert,F., Rice,P., Scudamore,C.L., Wells,S., Williams,R.,
            Hough,T.A. and Cox,I.J.
  TITLE     (1)H NMR metabolic profiling of plasma reveals additional
            phenotypes in knockout mouse models
  JOURNAL   J Proteome Res 14 (5), 2036-2045 (2015)
   PUBMED   25849460
REFERENCE   4  (bases 1 to 2724)
  AUTHORS   Kiss,G., Konrad,C., Pour-Ghaz,I., Mansour,J.J., Nemeth,B.,
            Starkov,A.A., Adam-Vizi,V. and Chinopoulos,C.
  TITLE     Mitochondrial diaphorases as NAD donors to segments of the citric
            acid cycle that support substrate-level phosphorylation yielding
            ATP during respiratory inhibition
  JOURNAL   FASEB J 28 (4), 1682-1697 (2014)
   PUBMED   24391134
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK147952.1, AK085272.1 and AL513346.17.
            
            On Mar 31, 2011 this sequence version replaced NM_177216.3.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the central coding region, compared to variant 1.
            This results in a shorter protein (isoform 2), compared to isoform
            1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK085272.1, BC107239.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                AK147952.1         2-37
            37-1214             AK085272.1         2-1179
            1215-2724           AL513346.17        193106-194615       c
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 57.21 cM"
     gene            1..2724
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /note="cytochrome b5 reductase 2"
                     /db_xref="GeneID:320635"
                     /db_xref="MGI:MGI:2444415"
     exon            1..75
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            76..202
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             125..955
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /EC_number="1.6.2.2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     NADH-cytochrome b5 reductase 2; cytochrome b5 reductase
                     b5R.2"
                     /codon_start=1
                     /product="NADH-cytochrome b5 reductase 2 isoform 2"
                     /protein_id="NP_796190.1"
                     /db_xref="CCDS:CCDS85373.1"
                     /db_xref="GeneID:320635"
                     /db_xref="MGI:MGI:2444415"
                     /translation="MSVKKKDLITLQDPEAKYPLPLIEKEQISHNTRRFRFGLPSPDH
                     VLGLPVGNYVHLLAQINNELVIRAYTPVSSDDDQGFVDLIIKIYFKNVHPKYPEGGKM
                     TQYLENMKIGDTILFRGPTGRLFYNEPGTLLIKANKTSEPEKKLVHHLGMIAGGTGIT
                     PMLQLIRHITKDTSDETRMSLLFANQTEEDILLRKELEEVATTHHKQFNLWYTLDRPP
                     SDWKYSSGFVSADMIKEHLPPPGEDTLILVCGPPPLIQAAAHPSLEQLSYTKDMIFIY
                     "
     exon            203..275
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            276..382
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            383..512
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            513..596
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            597..682
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            683..782
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            783..2724
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1191..1196
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1215
                     /gene="Cyb5r2"
                     /gene_synonym="b5R.2; D630003K02Rik"
                     /note="major polyA site"
ORIGIN      
        1 actgccggat gtttcatcat ccccaccatc aaaggcactg ttcatctcga ggataagtca
       61 ggcatctcgc tccaggacca aacctgtcac cggggccact gcgattctca tagccctgaa
      121 gaaaatgagt gtcaagaaga aagatcttat caccttacag gaccctgaag ccaagtaccc
      181 gctgcccttg attgagaagg agcaaatcag tcacaacacc agaaggttcc gctttgggct
      241 gccctcacca gaccatgtcc tggggcttcc tgtcggtaac tatgtccatc tcttggcaca
      301 aattaacaat gaattggtga tcagggctta cacacctgtc tccagtgacg atgatcaagg
      361 ctttgtggac ttaattataa agatctactt caaaaatgtg caccccaagt atcctgaagg
      421 aggaaagatg actcagtact tggagaacat gaaaattggg gataccatcc tttttcgggg
      481 gccaacaggg cgcctgttct acaatgagcc aggaaccctt ttaatcaaag caaacaaaac
      541 aagtgagcct gaaaaaaagc tggttcacca cctggggatg attgctggag gcacaggcat
      601 tacgcccatg ctacagctga ttcgccacat caccaaggac accagcgatg agaccaggat
      661 gtccctcctc tttgctaacc agacagaaga ggatatcttg ctgagaaagg agctagaaga
      721 agtcgccacg actcatcaca aacagttcaa cctgtggtat accctggaca ggcctcccag
      781 tgactggaag tacagctcag gctttgttag cgctgacatg atcaaggagc acctccctcc
      841 tcctggggag gacacgctca tcctggtgtg tggcccacca cctctgatcc aggcggcagc
      901 tcaccccagc ctggagcagc tcagctatac caaggacatg atttttatct actaacacct
      961 cccacactac ttagcgggtc cctctctctg ctttagagtg agtttggctt caccctgttc
     1021 aagtcaggta tttgcaaggt gtctctccac gcacccttgg gcatgatgtt cttttggttg
     1081 tgggcctaag aagtcttcac aggctgaaga caaaagtgat ttttgagctt ccaggtttgg
     1141 aggctgggaa ggaagccact ttctccatga ctttctccat ggcttagcgg aataaactgg
     1201 actgctttgc atttagctca caatgtgtgg atatgtagaa aatacactgg gcagtccagg
     1261 acttgctgat gtgaggtgag ggtgaaaaaa gagtaggggt gtgaccagaa ccggcaatat
     1321 agcaatgttt acggatagca ttccagcagg atccgaacag gtctctatca acctaaccac
     1381 ctatgaacct tctttacatt tctctgcttt gaaggacaaa ctcacaagag ggcaaaggcc
     1441 ttgtgtggtc ttccttgcct tgtaagacac tttttttaaa aagctgtatg tcagggcagt
     1501 aaatccacag taggtgggct ccaggaatcc ctttctgact ccctgcattt tgtacagcag
     1561 atggcaacat tggggatatt tatattttag tatcctccac cttggcactg tgtcaaagtt
     1621 ggcatggtta agtttcttca tttcccattt cccagaagct gtgcttcaaa tcccatcatt
     1681 caaaccagac gatacaggga ccactattgc cacctgcagg tcaaagtaag aataacatac
     1741 tggtgctctg ctactgcagg ggttaaagct caccccaact ccattcctta taatcttcct
     1801 aatgggccag aggttctgcg gagcttttgt tctctaagtc acacaggagg aaacagacag
     1861 cgttagtcat gcatttcccc agccattcag acagtgggtg gcagagttgt ctttaaacct
     1921 ggatctgttt aattttatgt cctttcggaa gaactggcaa agtagctgca gtgttagcac
     1981 ttgctgcagg atttgctact caatgtgcgt gtgtgtgtgt gtgtgtgtgc atacgggagg
     2041 gtggggggta caagaaaaaa aaaaagagag gaatggggtt ccctggagct ctgctatatc
     2101 cttgaatctc acccacaggg gaccaaactc cgtctcaaac aggccaggga gggacactcc
     2161 cacgttcatt cacccaattg tactcagtgt attctttcat aatgacctcc tcggggaaag
     2221 acacaacggc tcactctctg ctacattgcc ccgcttagcc ttgaactcac tctgtagctc
     2281 aagtcatctg tgaaccccac aacccttcta tctcagcttt ccaactaata ataactagga
     2341 ttacaagcat gtgccaccag ggtcagctgc aaagcctttc accactggac tcattccaca
     2401 tcattggatc tttcattccg tagaccttct ggtgatttca ctcgtcactg gacaaagcct
     2461 ggagatgctt ttggctttcc tagctagagg aggtgccaat gacatctgca cggaagacca
     2521 aggatagtgg tgactggcaa cttgattaaa agcagtacac ttggaactca cttaggccac
     2581 acagactaca gcagctcagc tggtttgtgt atcgggtaca catgtgcata taaccttaaa
     2641 gtgaactgtg aacatctcag aggcatacaa tgctgaaggg caaagtggat ttggaaccaa
     2701 aagaaagaat aaaggaagtg aagc
//
