LOCUS       NM_028057               1835 bp    mRNA    linear   ROD 31-MAY-2024
DEFINITION  Mus musculus cytochrome b5 reductase 1 (Cyb5r1), transcript variant
            1, mRNA.
ACCESSION   NM_028057
VERSION     NM_028057.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Faber,J.E., Zhang,H., Xenakis,J.G., Bell,T.A., Hock,P.,
            Pardo-Manuel de Villena,F., Ferris,M.T. and Rzechorzek,W.
  TITLE     Large differences in collateral blood vessel abundance among
            individuals arise from multiple genetic variants
  JOURNAL   J Cereb Blood Flow Metab 43 (11), 1983-2004 (2023)
   PUBMED   37572089
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Yan,B., Ai,Y., Sun,Q., Ma,Y., Cao,Y., Wang,J., Zhang,Z. and Wang,X.
  TITLE     Membrane Damage during Ferroptosis Is Caused by Oxidation of
            Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1
  JOURNAL   Mol Cell 81 (2), 355-369 (2021)
   PUBMED   33321093
  REMARK    GeneRIF: Membrane Damage during Ferroptosis Is Caused by Oxidation
            of Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1.
REFERENCE   3  (bases 1 to 1835)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC131592.10 and AK005159.1.
            
            On Sep 8, 2017 this sequence version replaced NM_028057.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK005159.1, BC024618.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               AC131592.10        77536-77744
            210-1835            AK005159.1         2-1627
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 58.21 cM"
     gene            1..1835
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /note="cytochrome b5 reductase 1"
                     /db_xref="GeneID:72017"
                     /db_xref="MGI:MGI:1919267"
     exon            1..273
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     CDS             259..1176
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /EC_number="1.6.2.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NAD(P)H:quinone oxidoreductase type 3, polypeptide A2;
                     NADH-cytochrome b5 reductase 1"
                     /codon_start=1
                     /product="NADH-cytochrome b5 reductase 1 isoform 1"
                     /protein_id="NP_082333.1"
                     /db_xref="CCDS:CCDS15308.1"
                     /db_xref="GeneID:72017"
                     /db_xref="MGI:MGI:1919267"
                     /translation="MGIQPSPVLLASLGVGLLTLLGLALGTYLVRRSRRPQVTLQDPD
                     EKYLLRLLDKTTVSHNTRRFRFALPTAHHILGLPVGKHVYLSARIDGSLVIRPYTPVT
                     SDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKIGDMVEFRGPSGLLSYAGKGN
                     FNIQPNKKSPPELRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFLLFANQTER
                     DIILREDLEELQAQYPNRFKLWFTLDSPPEDWTYSKGFVTADMIQEHLPAPAEDVLLL
                     LCGPPPMVQLACHPNLDKLGYSQKMRFTY"
     exon            274..423
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            424..496
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            497..603
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            604..733
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            734..817
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            818..903
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1003
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1835
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1802..1807
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /note="hexamer: AATAAA"
     polyA_site      1824
                     /gene="Cyb5r1"
                     /gene_synonym="1500005G05Rik; B5R.1; Nqo3a2"
                     /note="major polyA site"
ORIGIN      
        1 aaaactgtca tcctttgggg taggtctaag ttgcagagag aagaaagctc tcaaaaggag
       61 ggcctgagac ggccaagata cccacgccct tggaccacct actccgcacg gaagaggggg
      121 gggcatagtc cccgctagtc ctgcctcagt caggccctcc tccatagacc acgccccgga
      181 gagctccgcc tccgggcccg ccccgcggcg cattgtggga tctttccgcc ccaccggagc
      241 gggacgcacc tctaggtcat ggggatccag ccgagcccgg tcttgctagc ctctctgggg
      301 gtggggctgc ttactctgct cggcctggct ctgggtacct acttggttcg aagatcccgc
      361 cggccgcagg ttactctcca ggacccggat gagaagtacc tgctgcgatt gctagacaag
      421 acgacagtga gccacaacac caggaggttc cgctttgcct tgcccaccgc ccaccacatt
      481 ctggggctgc ctgtgggtaa gcacgtctac ctctctgccc gaattgatgg cagtctggtc
      541 atcaggcctt acacccctgt caccagtgat gaagaccaag gctatgtaga tcttgtcatc
      601 aaggtttatc tgaaaggtgt gcaccccaaa tttcctgaag gagggaagat gtctcaatac
      661 ctggatagcc tgaagattgg ggatatggtg gagttccggg ggccgagcgg gttgctcagt
      721 tatgctggga aagggaattt taatattcag cccaacaaaa aatctccacc tgaactgcga
      781 gtggcgaaga aattgggaat gattgctggt gggacaggaa tcaccccaat gctgcagctg
      841 atccgggcca tcttgaaagt ccctgaagat ccaacccagt gctttctgct ttttgccaac
      901 cagactgaaa gggatataat cctgcgggag gacctagagg aacttcaggc ccagtatcct
      961 aatcgcttta agctctggtt cactctggac tctcctccag aagactggac ctacagcaag
     1021 ggctttgtta cggctgacat gatccaggag cacctgcctg ccccagcaga ggatgtgctg
     1081 ctgctgctct gtggaccacc acccatggtg cagctggcct gccaccctaa cttggataag
     1141 ctgggctatt cacaaaagat gcgattcacc tactgagctt cgcaaagcct ccctcacccc
     1201 atgtgtgcag ttgtttctag tcgattcttg aataccaaga ggccctaggg cctttgctga
     1261 gagcatcagc cttctgtgtc tcatcccgga atctgggtgc ttgcagaaac aacactccct
     1321 cagctatggt ggatgcaggt caaggctaag tcactccctt gaagcttcct ttatcctaac
     1381 taatgtcctt gtgataaaag gtccagatga ggctcaagga gcagtctcta gagccagaaa
     1441 gaaggaagtg tgtgtacatt tgttatgagg ctgcctagtt tctgagagca ttatggtcac
     1501 ctctgtgtct gtaatgaaag caatggacag ggcaatgcat ttcgctccgt gttcagcact
     1561 tcatccacta cttagatgag tacaggttga gtgcgcttat agacacagtc ctacagaaac
     1621 tgtaggaagg gaattatttt ccggaattct agacctcaga gaaaggaggc tggtgtgtgt
     1681 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtat ctcaggccct gcccaggata
     1741 gcgtgaaatg attactcata acttaagatt gctgcctgta caaagtgtgt ctggctgttc
     1801 aaataaatgg agccgaggcc ctaatgatct caagg
//
