LOCUS       NM_025558               4294 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cytochrome b5 type B (Cyb5b), mRNA.
ACCESSION   NM_025558
VERSION     NM_025558.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Kurogi,S., Sekimoto,T., Funamoto,T., Ota,T., Nakamura,S., Nagai,T.,
            Nakahara,M., Yoshinobu,K., Araki,K., Araki,M. and Chosa,E.
  TITLE     Development of an efficient screening system to identify novel bone
            metabolism-related genes using the exchangeable gene trap
            mutagenesis mouse models
  JOURNAL   Sci Rep 7, 40692 (2017)
   PUBMED   28106071
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4294)
  AUTHORS   Jakobs,H.H., Mikula,M., Havemeyer,A., Strzalkowska,A.,
            Borowa-Chmielak,M., Dzwonek,A., Gajewska,M., Hennig,E.E.,
            Ostrowski,J. and Clement,B.
  TITLE     The N-reductive system composed of mitochondrial amidoxime reducing
            component (mARC), cytochrome b5 (CYB5B) and cytochrome b5 reductase
            (CYB5R) is regulated by fasting and high fat diet in mice
  JOURNAL   PLoS One 9 (8), e105371 (2014)
   PUBMED   25144769
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4294)
  AUTHORS   Ismail,H.M., O'Neill,P.M., Hong,D.W., Finn,R.D., Henderson,C.J.,
            Wright,A.T., Cravatt,B.F., Hemingway,J. and Paine,M.J.
  TITLE     Pyrethroid activity-based probes for profiling cytochrome P450
            activities associated with insecticide interactions
  JOURNAL   Proc Natl Acad Sci U S A 110 (49), 19766-19771 (2013)
   PUBMED   24248381
REFERENCE   5  (bases 1 to 4294)
  AUTHORS   Nakahara,M., Tateyama,H., Araki,M., Nakagata,N., Yamamura,K. and
            Araki,K.
  TITLE     Gene-trap mutagenesis using Mol/MSM-1 embryonic stem cells from
            MSM/Ms mice
  JOURNAL   Mamm Genome 24 (5-6), 228-239 (2013)
   PUBMED   23604909
REFERENCE   6  (bases 1 to 4294)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   7  (bases 1 to 4294)
  AUTHORS   Oikawa,K., Kimura,S., Aoki,N., Atsuta,Y., Takiyama,Y., Nagato,T.,
            Yanai,M., Kobayashi,H., Sato,K., Sasajima,T. and Tateno,M.
  TITLE     Neuronal calcium sensor protein visinin-like protein-3 interacts
            with microsomal cytochrome b5 in a Ca2+-dependent manner
  JOURNAL   J Biol Chem 279 (15), 15142-15152 (2004)
   PUBMED   14739275
  REMARK    GeneRIF: microsomal cytochrome b5 interacts with neuronal calcium
            sensor protein visinin-like protein-3 in a Ca2+-dependent manner
REFERENCE   8  (bases 1 to 4294)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   9  (bases 1 to 4294)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 4294)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125207.5.
            
            On Oct 19, 2007 this sequence version replaced NM_025558.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK168422.1, AK052810.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-213               AC125207.5         31590-31802
            214-342             AC125207.5         50740-50868
            343-372             AC125207.5         51320-51349
            373-401             AC125207.5         60343-60371
            402-4294            AC125207.5         64507-68399
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 53.71 cM"
     gene            1..4294
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /note="cytochrome b5 type B"
                     /db_xref="GeneID:66427"
                     /db_xref="MGI:MGI:1913677"
     exon            1..213
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /inference="alignment:Splign:2.1.0"
     CDS             52..492
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /codon_start=1
                     /product="cytochrome b5 type B precursor"
                     /protein_id="NP_079834.2"
                     /db_xref="CCDS:CCDS22647.1"
                     /db_xref="GeneID:66427"
                     /db_xref="MGI:MGI:1913677"
                     /translation="MATPEASGSGEKVEGSEPSVTYYRLEEVAKRNSAEETWMVIHGR
                     VYDITRFLSEHPGGEEVLLEQAGADATESFEDVGHSPDAREMLKQYYIGDVHPSDLKP
                     KGDDKDPSKNNSCQSSWAYWFVPIVGAILIGFLYRHFWADSKSS"
     exon            214..342
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /inference="alignment:Splign:2.1.0"
     exon            343..372
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /inference="alignment:Splign:2.1.0"
     exon            373..401
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /inference="alignment:Splign:2.1.0"
     exon            402..4294
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /inference="alignment:Splign:2.1.0"
     regulatory      901..906
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /note="hexamer: AGTAAA"
     polyA_site      925
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /note="major polyA site"
     regulatory      2750..2755
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /note="hexamer: AATAAA"
     polyA_site      2774
                     /gene="Cyb5b"
                     /gene_synonym="1810044O22Rik; Cyb5m"
                     /note="major polyA site"
ORIGIN      
        1 aggaagttag cggtggaagc ccagatagcg gcggagaggg cttcggatcc catggcgacc
       61 ccggaggcca gcggcagtgg cgagaaggtc gagggctccg agccctccgt cacctactac
      121 cggctggagg aggtggcgaa gcgaaactcg gcggaagaga cgtggatggt gatccacggg
      181 cgagtctacg atatcacccg cttcctcagc gagcatcctg gcggggaaga ggttctgctg
      241 gaacaagcgg gtgctgatgc aactgaaagc tttgaagatg tcggccactc ccctgatgcc
      301 agggagatgc taaagcagta ctacattgga gatgtccatc cgagtgacct taaacctaaa
      361 ggcgatgaca aggatccttc caagaacaat tcatgccaaa gttcctgggc gtattggttt
      421 gtccccatcg tgggcgctat tcttataggt ttcttgtatc gtcacttctg ggctgacagc
      481 aaatcctcct gaggagacct cgttgaagtg cggagcacgt gctcctgcga tgagaggagg
      541 agacttgtgt ggaggctgca gggtgttccc tccttggaac ctgccagttg ttttctttcc
      601 ccttggagcc aagatggttg accagatatc tatcctcctt cagtgtacga ccagagtctt
      661 tcatccgcca gagcctgacg cccaaagcac ctgctcactc tgtgtccttg ccagggtttc
      721 tctcattggt tcccactcct tcctttcatt tcaaaagttg atgccaatga cttagagttc
      781 ctatgttgta gtgacatggc ctttggggac agtacatgat ttctcataat ttgaattcat
      841 agtagatata gttaagggaa cagcagtcag gttatgaatc ttttcccatt tttcttttta
      901 agtaaattta tttttttaat tctgatttaa ttttgtcatt catcataagg gatttaattc
      961 atgaagttgt gtgtatctta tttcaaacac agctgccaga tgatcttatt tatgattgtt
     1021 agaagtaagg ggccagcaag atggctcagt ggataaaagc aattgccgcg aagcctgatg
     1081 acctgtgtct gagtcactgc actcacagga tagaaagaac cgattcccaa aagttgtcct
     1141 ctgacttcca catgtgtgct atggtctttg cacacacacg cacacacaca caccccagat
     1201 gttattttta aaaatgaagt acagcaacag tggctatcta cccctgcccc ctccagacag
     1261 tgtagctgat ggctcctcct gtctctaagt cccgcctctt ttcccaaaat acttcacaaa
     1321 gtgtgcatcc ctttgatttc ttaggaatct aagagctgaa gaaggactga cttcagagta
     1381 atctctttta caaagagaac caaacactac ttacaggaga gcagtttaaa aagggaccaa
     1441 catgcgctct ctggtctgac actggtttct aaccatcttc caccctggct gtctttacca
     1501 gtgaaagatg tgtgtgtgtg cttaccttct atacacttct ctccggtccg tggggcttca
     1561 caaaggcctt tctgctgttt ttctttaccg attagcaaat ttctgattcc ttgtcttaaa
     1621 gtacaagact caagtttgaa cttatgttta tctcagtaaa ataacttaga tgtaaatgtc
     1681 attgtccggc atgccccttc agattgggag accatgccaa gagacttcgg ttttcatctg
     1741 ggttttacct ccatggcgcc aacttttggt tttgtcctac tttctattga gggtaatgat
     1801 aaatctggaa gctttctttc ctcttggata tgaatttcac tggaaagtag gcagtcattt
     1861 aaaattcctt ctctcgttgg tccaggcatt ttcctggcac ctctgactgt attctctctc
     1921 cctgtggcag tgcctgacag tacccagctg tttcttagtt tctatgatct ttcttcacat
     1981 ctttgttcat taccagtcag cttctgggct ggtgtcacat ccagcaggaa accatgggat
     2041 tccagcagga ccagagatgt tgggaagtgt cctcctgcct cccttccagc ttgccagggg
     2101 cttgacagga caggaaactg cacagtaaac cagtctgccc gtgtaaacta ttttttaaaa
     2161 agttcagtcc aggactcgta tcctgtgatt tagcagttga atgaattcag aattcatcaa
     2221 aataacatta accttttcct tacacacttg aatatgatat tttagagtga tttctaggtg
     2281 acttttatgt cagagatgaa gacttaatca accattcgtc tttagaaacg tctgtaatat
     2341 tgtgtcatag attgacagcg taattagtct gtaacaaaag cggcatgcgg cgcgcatctc
     2401 caggtaaagg cagggaaggt tgtgtgcagg gagcccggga cctgcatgct gggatagcga
     2461 agtcacatgg agtatgctgg gtagaaggga agaacaaagg aacattgagt gcaggacagg
     2521 agcgtcccag ctaactccaa acttgatggg acacctagtg aaaaacatct ggcttgtagg
     2581 aaatgttaaa gaactgcatt ttcggctggg catggtggtg cacaccatta atcccgatgt
     2641 aggagagaca gaggctgcag aatctctgag ttgaggccag tctggtccac atagtaagtt
     2701 ccaggacagt caggctacat agagagacca tgtcacaacc actccaccaa ataaaagaac
     2761 tgtattctta agtagaatcg gtgttttgat atttctacag taaacatcaa taccaaaagc
     2821 cgttttttct atttcacatt tgtgtctttc ccagcttctt atagctgagc tttttctccc
     2881 gagagccaga aggaaaagat tttcatactt ttgtctttgc gatccatgaa atccgtcaat
     2941 acccagggct tcttttgaac tactgataat gtaatgtcat tttatattat ataaaaagga
     3001 tgcttttttt tttttaaatc tttaatccca gcacttggga ggcagaagca gacagttctc
     3061 ttgggttgta ggtcagcctg gtctacagag caagctccaa gccagccaaa gctacataat
     3121 gagattctgt tcaggtctac ttgttgtaat cctgttgcct acataaacag ctaggggaaa
     3181 ggtaggtttt aggtgatcgt ttgtcaaggc tttccgtaaa cggcaaagca acacaacatg
     3241 tgcacaggtg tacttttaaa agtctaaact tgtcaaagaa gcctggacat ttgagggtca
     3301 tgaaaaaaca ttaagaccaa gaggaggcag ttcctatcca ctgacagcac ctcctgacct
     3361 gtgggcgtgg gcaggtttga ttgtttattc ttgaggtgac cagctgctgc cacatataca
     3421 ggtaatggca ggtgaacatg agagatttgt gctacccaga taaaatgtgt taacacccat
     3481 tctctaaaag tttctaccca gccctgggag ctggttttca tcactgagtg atgtacagaa
     3541 agcaatagca cacagtgtat ggacttactt aagcagagac aagtctgtcc agagctgtca
     3601 cttgagctgg ctggctcttc cagcagttgc catcggggct cacggtgtag ggtttgtgct
     3661 cattaagtgc tgatggcaag cagtggaaag tgatttacaa gcaaggtgtg tcatcacatg
     3721 cagcactcat gcttctgtct ccagtgatgc ccgtccggct gaagtcgacc tgagatgact
     3781 gaagcaagga tcgtaaatgt tcattgcctc ctaccccaag ggaagtggac tcagacatgt
     3841 cttgtctcaa cgagaaaccg aagggttgaa gctgtgagat tcagttgcca aatatcccaa
     3901 aagtaacggg gagctccgtc acatttggcc agctttggga tttgtgggag tggtctagga
     3961 gggaggctga agaagcaggg gtgtgccagt cacctgcctt acacccagtg tcctactcat
     4021 gaatggcagg ggccactcct tgcccacgcc tgcctcgtct aatcagctgc ttgggcagac
     4081 gctcctttcg gttatttggc ttgtcttttc ctgcaccctc aactgagtgg ctaatactac
     4141 attccttttg tgaattcaac gatggggttt gtttacctat ttggtttcag cttattttga
     4201 acaaagatct ttgtctcttt ctactggaat gtgcacacag tgaatgtttg ttagaactac
     4261 acaataaaga tactgttttc cttttcttgt cctg
//
