LOCUS       NM_025797                833 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus cytochrome b5 type A (microsomal) (Cyb5a), transcript
            variant 1, mRNA.
ACCESSION   NM_025797
VERSION     NM_025797.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Chino,H., Hatta,T., Natsume,T. and Mizushima,N.
  TITLE     Intrinsically Disordered Protein TEX264 Mediates ER-phagy
  JOURNAL   Mol Cell 74 (5), 909-921 (2019)
   PUBMED   31006538
REFERENCE   2  (bases 1 to 833)
  AUTHORS   Sondhi,V., Owen,B.M., Liu,J., Chomic,R., Kliewer,S.A., Hughes,B.A.,
            Arlt,W., Mangelsdorf,D.J. and Auchus,R.J.
  TITLE     Impaired 17,20-Lyase Activity in Male Mice Lacking Cytochrome b5 in
            Leydig Cells
  JOURNAL   Mol Endocrinol 30 (4), 469-478 (2016)
   PUBMED   26974035
  REMARK    GeneRIF: Demonstration of the physiologic importance of cytochrome
            b5 activating the 17,20-lyase reaction and the production of
            19-carbon sex steroids from 21-carbon precursors in mice.
REFERENCE   3  (bases 1 to 833)
  AUTHORS   Plitzko,B., Ott,G., Reichmann,D., Henderson,C.J., Wolf,C.R.,
            Mendel,R., Bittner,F., Clement,B. and Havemeyer,A.
  TITLE     The involvement of mitochondrial amidoxime reducing components 1
            and 2 and mitochondrial cytochrome b5 in N-reductive metabolism in
            human cells
  JOURNAL   J Biol Chem 288 (28), 20228-20237 (2013)
   PUBMED   23703616
REFERENCE   4  (bases 1 to 833)
  AUTHORS   Yagita,Y., Hiromasa,T. and Fujiki,Y.
  TITLE     Tail-anchored PEX26 targets peroxisomes via a PEX19-dependent and
            TRC40-independent class I pathway
  JOURNAL   J Cell Biol 200 (5), 651-666 (2013)
   PUBMED   23460677
REFERENCE   5  (bases 1 to 833)
  AUTHORS   Menoret,A., Kumar,S. and Vella,A.T.
  TITLE     Cytochrome b5 and cytokeratin 17 are biomarkers in bronchoalveolar
            fluid signifying onset of acute lung injury
  JOURNAL   PLoS One 7 (7), e40184 (2012)
   PUBMED   22792238
  REMARK    GeneRIF: Cytochrome b5 and cytokeratin 17 are biomarkers in
            bronchoalveolar fluid signifying onset of acute lung injury.
REFERENCE   6  (bases 1 to 833)
  AUTHORS   Oikawa,K., Kimura,S., Aoki,N., Atsuta,Y., Takiyama,Y., Nagato,T.,
            Yanai,M., Kobayashi,H., Sato,K., Sasajima,T. and Tateno,M.
  TITLE     Neuronal calcium sensor protein visinin-like protein-3 interacts
            with microsomal cytochrome b5 in a Ca2+-dependent manner
  JOURNAL   J Biol Chem 279 (15), 15142-15152 (2004)
   PUBMED   14739275
REFERENCE   7  (bases 1 to 833)
  AUTHORS   Powles,N., Babbs,C., Ficker,M., Schimmang,T. and Maconochie,M.
  TITLE     Identification and analysis of genes from the mouse otic vesicle
            and their association with developmental subprocesses through in
            situ hybridization
  JOURNAL   Dev Biol 268 (1), 24-38 (2004)
   PUBMED   15031102
REFERENCE   8  (bases 1 to 833)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   9  (bases 1 to 833)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 833)
  AUTHORS   Hanlon,M.R., Begum,R.R., Newbold,R.J., Whitford,D. and Wallace,B.A.
  TITLE     In vitro membrane-inserted conformation of the cytochrome b(5) tail
  JOURNAL   Biochem J 352 Pt 1 (Pt 1), 117-124 (2000)
   PUBMED   11062064
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY284726.1, AK132102.1 and AC134545.5.
            
            On Feb 8, 2017 this sequence version replaced NM_025797.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BF538634.1, DV046952.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                BY284726.1         1-53
            54-824              AK132102.1         1-771
            825-833             AC134545.5         91884-91892         c
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 57.53 cM"
     gene            1..833
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /note="cytochrome b5 type A (microsomal)"
                     /db_xref="GeneID:109672"
                     /db_xref="MGI:MGI:1926952"
     exon            1..270
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /inference="alignment:Splign:2.1.0"
     CDS             142..546
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="cytochrome b5 isoform 1"
                     /protein_id="NP_080073.1"
                     /db_xref="CCDS:CCDS29385.1"
                     /db_xref="GeneID:109672"
                     /db_xref="MGI:MGI:1926952"
                     /translation="MAGQSDKDVKYYTLEEIQKHKDSKSTWVILHHKVYDLTKFLEEH
                     PGGEEVLREQAGGDATENFEDVGHSTDARELSKTYIIGELHPDDRSKIAKPSDTLITT
                     VESNSSWWTNWVIPAISALAVALMYRLYMAED"
     exon            271..399
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /inference="alignment:Splign:2.1.0"
     exon            400..429
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /inference="alignment:Splign:2.1.0"
     exon            430..464
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /inference="alignment:Splign:2.1.0"
     exon            465..833
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      805..810
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /note="hexamer: AATATA"
     polyA_site      826
                     /gene="Cyb5a"
                     /gene_synonym="0610009N12Rik; Cyb5"
                     /note="major polyA site"
ORIGIN      
        1 agtttgaggc accgccctct cctggttctg ggcacagggc ccggtggagc tgggaatgtc
       61 ccgggcggag gcgctcagac ctgatcatag cacaaccctg cacggctgtg ttgcagggcc
      121 cggtagactc actgttccga gatggccggg cagtcagaca aggatgtgaa gtactacacc
      181 ctggaagaga ttcagaagca caaagacagc aagagcacct gggtgatcct gcatcataag
      241 gtgtacgatc tgaccaagtt tctcgaagag catcctggtg gagaagaagt cctaagagag
      301 caagctgggg gtgatgctac cgagaatttt gaggacgtcg ggcactctac ggatgcacga
      361 gaactgtcca aaacatacat catcggggaa ctccatccag atgacagatc aaagatagcc
      421 aagccttcgg atactcttat cactactgtg gagtctaatt ccagttggtg gaccaactgg
      481 gtgatcccag ccatctcagc cctggctgta gctctgatgt atcgcctcta catggcagaa
      541 gattgacttg tctctccgaa gccgaagaac taaaagactg ccccagagcg gggagaaaag
      601 aagccagtgt taatcacttc cactgacaga aacctccccc tgagaacgta attgtaatat
      661 atctgtttcc ctttcctcct gtgctagtag aacaaacaag ggactctttg tactcttcaa
      721 cttttcaaat gtgccttttt actcaacttc atggtgatgt ttcttcacta cataatttac
      781 ttattgtaaa cacgatcttt ttaaaatata tctggcttgt aaagtatacc ata
//
