LOCUS       NM_001357289            1726 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus cytochrome b-561 domain containing 2 (Cyb561d2),
            transcript variant 2, mRNA.
ACCESSION   NM_001357289 XM_006511775
VERSION     NM_001357289.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   3  (bases 1 to 1726)
  AUTHORS   Berczi,A., Desmet,F., Van Doorslaer,S. and Asard,H.
  TITLE     Spectral characterization of the recombinant mouse tumor suppressor
            101F6 protein
  JOURNAL   Eur Biophys J 39 (8), 1129-1142 (2010)
   PUBMED   19943161
  REMARK    GeneRIF: show (1) TSCytb's reduced spectra have a split a-band at
            already low ascorbate concentrations (at already high potential
            levels), and (2) it has two heme b redox centers, both with
            positive redox potentials, that differ by about 100 mV from each
            other
REFERENCE   4  (bases 1 to 1726)
  AUTHORS   Mizutani,A., Sanuki,R., Kakimoto,K., Kojo,S. and Taketani,S.
  TITLE     Involvement of 101F6, a homologue of cytochrome b561, in the
            reduction of ferric ions
  JOURNAL   J Biochem 142 (6), 699-705 (2007)
   PUBMED   17938141
  REMARK    GeneRIF: 101F6 exhibited ferric as well as azo-dye-reductase
            activity.
REFERENCE   5  (bases 1 to 1726)
  AUTHORS   Lerman,M.I. and Minna,J.D.
  TITLE     The 630-kb lung cancer homozygous deletion region on human
            chromosome 3p21.3: identification and evaluation of the resident
            candidate tumor suppressor genes. The International Lung Cancer
            Chromosome 3p21.3 Tumor Suppressor Gene Consortium
  JOURNAL   Cancer Res 60 (21), 6116-6133 (2000)
   PUBMED   11085536
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672219.7 and AF131206.1.
            
            [WARNING] On Oct 26, 2024 this sequence was replaced by
            NM_001357289.2.
            
            On Oct 25, 2017 this sequence version replaced XM_006511775.3.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BG919231.1, BC027212.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-82                AL672219.7         115261-115342       c
            83-1177             AF131206.1         11-1105
            1178-1726           AL672219.7         112160-112708       c
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 58.12 cM"
     gene            1..1726
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /note="cytochrome b-561 domain containing 2"
                     /db_xref="GeneID:56368"
                     /db_xref="MGI:MGI:1929280"
     exon            1..159
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /inference="alignment:Splign:2.1.0"
     exon            160..313
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /inference="alignment:Splign:2.1.0"
     CDS             187..855
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /EC_number="7.2.1.3"
                     /note="isoform a is encoded by transcript variant 2; tumor
                     suppressor, region 10; gene for 101F6 protein; cytochrome
                     b561 domain-containing protein 2; putative tumor
                     suppressor protein 101F6; transmembrane ascorbate
                     ferrireductase; transmembrane reductase CYB561D2"
                     /codon_start=1
                     /product="transmembrane reductase CYB561D2 isoform a"
                     /protein_id="NP_001344218.1"
                     /db_xref="CCDS:CCDS23491.1"
                     /db_xref="GeneID:56368"
                     /db_xref="MGI:MGI:1929280"
                     /translation="MALSVETESHIYRALRTASGAAAHLVALGFTIFVAVLARPGSSL
                     FSWHPVLMSLAFSFLMTEALLMFSPESSLLRSLSRKVRARCHWVLQLLALLCALLGLG
                     LVILHKEQLGKAHLTTRHGQAGLLAVLWAGLQCSGGMGLLYPKLLPRWPLAKLKLYHA
                     TSGLVGYLLGSASLLLGMFSLWFTATVTGGAWYLAVLCPILTSLVIMNQVSNAYLYRK
                     RIQP"
     exon            314..351
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /inference="alignment:Splign:2.1.0"
     exon            352..1726
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1139..1144
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /note="hexamer: AATAAA"
     regulatory      1143..1148
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /note="hexamer: AACAAA"
     polyA_site      1157
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
     polyA_site      1162
                     /gene="Cyb561d2"
                     /gene_synonym="Tsp10"
                     /note="major polyA site"
ORIGIN      
        1 gctggatggg atgggagggg gcttgtccca ggaagtctcg ggtgggtaga catcgcaccc
       61 ggaagtgaag ctactccgta acggagcggt ggcgcggctg gcggggccga gggtccagtt
      121 tattttggag gaagtcactg cgtagaacct gagaagcagg ctgtgcagct atcggctggg
      181 ctgaccatgg ccctttctgt ggagacggag tcgcatatct accgagctct gcgcactgcc
      241 tctggggctg cagcccacct cgtggcgtta ggctttacca tctttgttgc tgtgcttgcc
      301 agacctggct ccagtctgtt ctcctggcac cccgttctta tgtctctggc tttctccttc
      361 ctgatgaccg aggcactcct gatgttctct cctgagagtt ccctgctgcg gtccctctct
      421 cggaaggtcc gcgcacgctg ccactgggtg ctccagctgc tggccctact ctgtgctctg
      481 ctgggtctag gtcttgtcat cctccacaaa gagcagcttg gcaaagcaca cctgaccact
      541 cggcatgggc aggcagggct gttagctgtg ctgtgggcag gtctgcagtg ttcagggggc
      601 atggggctgc tctaccccaa gttgctgccc cgatggcccc tggcaaagct gaaactctac
      661 cacgccactt ctgggttggt gggctacctg ctgggcagtg ccagcctcct gttgggcatg
      721 ttctcactct ggttcactgc cactgtcact ggtggtgcct ggtacctggc tgtgttgtgc
      781 cccatcctca ccagtttggt aatcatgaac caagtgagca atgcctacct gtaccgcaag
      841 aggatccagc catgagcact caccagttat tacgaaggct ggatttgcac ctcagcttcg
      901 gaccaggagc tcggaacctg ttggactctc acaactgaca gggtctgggg acatttcagg
      961 gactagggca ctcaggggta gatctcctca cactagagtg tctccctttt cttgctgtca
     1021 acccagggga gcatggtcat gtgtctgggt gaagttggag ttgcaacagt ctccccagca
     1081 gccatacagc ttatatttgt actggtatgt ccagaaatca tggaggaaag aaaagtaaaa
     1141 taaacaaaca gaatcctgaa aacctgtcgg gaggagcagc gcagagttga ggatggttct
     1201 tgctaatgca tcatggtaag aaaatggtca gctgggtaag gaggggcccc tccttgaatg
     1261 ggagtcctca gagcctgctg tgaaacttcc tctgatccca gtgcattggg gggctaagtg
     1321 tggacggcac agctatttcc agctaattgt gctagagtct ggagactatg gccagtgaca
     1381 ggagatggac ttagaagttg aaccatggtc atttgtggtg gcgggcagat ctctgtgagc
     1441 tctaagccaa ccaaggctac atagtaagat cctgtctcaa aagggaaaaa gaaagtagaa
     1501 tcatgaacaa gagaaatttg ccttctgttt ctgagacaag gtttcaagtc tcccaggtgg
     1561 gctttgaact cacaacatgg cctaggatga cagacttcag attctcctgt ttcaccgcag
     1621 tgctaggatt atagatgtat gtaccatgta tgtccatacc atgccccact tatgtagtgc
     1681 tgggacccaa acccagagct ctgaaatgct aagcaagtgt tctgtc
//
