LOCUS       NM_028288               5020 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus cullin 4B (Cul4b), transcript variant 1, mRNA.
ACCESSION   NM_028288
VERSION     NM_028288.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Guo,B., Zheng,Y., Fan,Y., Yang,Y., Wang,Y., Qin,L., An,Y., Xu,X.,
            Zhang,X., Sun,G., Dou,H., Shao,C., Gong,Y., Jiang,B. and Hu,H.
  TITLE     Enhanced ApcMin/+ adenoma formation after epithelial CUL4B deletion
            by recruitment of myeloid-derived suppressor cells
  JOURNAL   Neoplasia 53, 101005 (2024)
   PUBMED   38761506
  REMARK    GeneRIF: Enhanced Apc[Min/+] adenoma formation after epithelial
            CUL4B deletion by recruitment of myeloid-derived suppressor cells.
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Guo,B., Huo,X., Xie,X., Zhang,X., Lian,J., Zhang,X., Gong,Y.,
            Dou,H., Fan,Y., Mao,Y., Wang,J. and Hu,H.
  TITLE     Dynamic role of CUL4B in radiation-induced intestinal
            injury-regeneration
  JOURNAL   Sci Rep 14 (1), 9906 (2024)
   PUBMED   38689033
  REMARK    GeneRIF: Dynamic role of CUL4B in radiation-induced intestinal
            injury-regeneration.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5020)
  AUTHORS   Dar,A.A., Ortega,Y., Aktas,S., Wu,K., Guha,I., Porter,N., Rosen,S.,
            DeVita,R.J., Pan,Z.Q. and Oliver,P.M.
  TITLE     CRL4b Inhibition Ameliorates Experimental Autoimmune
            Encephalomyelitis Progression
  JOURNAL   J Immunol 212 (6), 982-991 (2024)
   PUBMED   38265261
REFERENCE   4  (bases 1 to 5020)
  AUTHORS   Zhang,L., Hao,P., Chen,X., Lv,S., Gao,W., Li,C., Li,Z. and Zhang,W.
  TITLE     CRL4B E3 ligase recruited by PRPF19 inhibits SARS-CoV-2 infection
            by targeting ORF6 for ubiquitin-dependent degradation
  JOURNAL   mBio 15 (2), e0307123 (2024)
   PUBMED   38265236
  REMARK    GeneRIF: CRL4B E3 ligase recruited by PRPF19 inhibits SARS-CoV-2
            infection by targeting ORF6 for ubiquitin-dependent degradation.
REFERENCE   5  (bases 1 to 5020)
  AUTHORS   Wang,Y., Manzi,M., Feswick,A., Renshaw,L., Oliver,P.M.,
            Tibbetts,S.A. and Moser,E.K.
  TITLE     B cell expression of E3 ubiquitin ligase Cul4b promotes chronic
            gammaherpesvirus infection in vivo
  JOURNAL   J Virol 97 (12), e0100823 (2023)
   PUBMED   37962378
  REMARK    GeneRIF: B cell expression of E3 ubiquitin ligase Cul4b promotes
            chronic gammaherpesvirus infection in vivo.
REFERENCE   6  (bases 1 to 5020)
  AUTHORS   Dooley,S., Hamzavi,J., Ciuclan,L., Godoy,P., Ilkavets,I.,
            Ehnert,S., Ueberham,E., Gebhardt,R., Kanzler,S., Geier,A.,
            Breitkopf,K., Weng,H. and Mertens,P.R.
  TITLE     Hepatocyte-specific Smad7 expression attenuates TGF-beta-mediated
            fibrogenesis and protects against liver damage
  JOURNAL   Gastroenterology 135 (2), 642-659 (2008)
   PUBMED   18602923
REFERENCE   7  (bases 1 to 5020)
  AUTHORS   Tripathi,R., Kota,S.K. and Srinivas,U.K.
  TITLE     Cullin4B/E3-ubiquitin ligase negatively regulates beta-catenin
  JOURNAL   J Biosci 32 (6), 1133-1138 (2007)
   PUBMED   17954973
  REMARK    GeneRIF: Plays a conserved role in the regulation of beta-catenin
            levels.
REFERENCE   8  (bases 1 to 5020)
  AUTHORS   Oda,M., Yamagiwa,A., Yamamoto,S., Nakayama,T., Tsumura,A.,
            Sasaki,H., Nakao,K., Li,E. and Okano,M.
  TITLE     DNA methylation regulates long-range gene silencing of an X-linked
            homeobox gene cluster in a lineage-specific manner
  JOURNAL   Genes Dev 20 (24), 3382-3394 (2006)
   PUBMED   17182866
REFERENCE   9  (bases 1 to 5020)
  AUTHORS   Tripathi,R., Sastry,K.S., Kota,S.K. and Srinivas,U.K.
  TITLE     Cloning and characterization of mouse cullin4B/E3 ubiquitin ligase
  JOURNAL   J Biosci 30 (3), 329-337 (2005)
   PUBMED   16052071
  REMARK    GeneRIF: The cloning and expression of amino and carboxy-terminal
            halves of cullin4B in mouse PCC4 cells is reported.
REFERENCE   10 (bases 1 to 5020)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK032701.1, AK160998.1, AK164640.1 and AI037712.1.
            
            On Oct 26, 2007 this sequence version replaced NM_028288.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY330868.1, AK032701.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-515               AK032701.1         2-516
            516-3342            AK160998.1         286-3112
            3343-3962           AK164640.1         719-1338
            3963-4995           AK164640.1         1340-2372
            4996-5020           AI037712.1         435-459
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 22.81 cM"
     gene            1..5020
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /note="cullin 4B"
                     /db_xref="GeneID:72584"
                     /db_xref="MGI:MGI:1919834"
     exon            1..58
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            59..133
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     CDS             73..2985
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /codon_start=1
                     /product="cullin-4B"
                     /protein_id="NP_082564.3"
                     /db_xref="CCDS:CCDS40948.1"
                     /db_xref="GeneID:72584"
                     /db_xref="MGI:MGI:1919834"
                     /translation="MSRSTRSKERRENDTDSEDNSSETSNQERRRCRQGPPRPPYPPL
                     LPPVFPPPTPPPQVRRTRGLQDLGAMKSVCPGTSGFSSPNPSAASAAAQEVRSATDGN
                     TSTTPPTSAKKRKLNSSSSSSNSSNEREDFDSTSSSSTPPQPRDSASPSTSSFCLGVP
                     VATSSHVPIQKKLRFEDTLEFVGIDTKMAEESSSSSSSSSPTAATSQQQQQQQLKTKS
                     ILISSVASVHHANGLAKSSTAVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQK
                     LKEAVEAIQNSTSIKYNLEELYQAVENLCSHKISANLYKQLRQICEDHIKAQIHQFRE
                     DSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAH
                     IISDQKVQTKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQQFLQET
                     NRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLIASVEKQLLGE
                     HLTAILQKGLNSLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGSTIVINP
                     EKDKTMVQELLDFKDKVDHIIDTCFLKNEKFINAMKEAFETFINKRPNKPAELIAKYV
                     DSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDA
                     EKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILT
                     MGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGKK
                     ELQVSLFQTMVLLMFNEGEEFSLEEIKHATGIEDGELRRTLQSLACGKARVLAKNPKG
                     KDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRI
                     MKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA"
     exon            134..307
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            308..853
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            854..969
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1073
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1143
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1217
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1218..1380
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1381..1470
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1553
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1554..1621
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1622..1740
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1933
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2038
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2149
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2235
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2343
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2457
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            2458..2563
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            2564..2736
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            2737..2889
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     exon            2890..5002
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3026..3031
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /note="hexamer: GATAAA"
     polyA_site      3049
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /note="major polyA site"
     regulatory      4979..4984
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /note="hexamer: AATAAA"
     polyA_site      5002
                     /gene="Cul4b"
                     /gene_synonym="2700050M05Rik; CUL-4B; mKIAA0695"
                     /note="major polyA site"
ORIGIN      
        1 agtctggtcc gggcgcagtc gacgacagac gtcttccaga aggtctctga ttcatgagcc
       61 atcaaagaga ggatgtcacg ttcaactagg tctaaagaaa gaagagagaa tgacaccgac
      121 tcagaagata acagttcgga aacctccaat caagagcgcc gaaggtgtcg ccaaggtccg
      181 ccccgccctc cctatccacc tcttttaccc cccgtgttcc ctcccccaac ccccccacct
      241 caagtccggc ggacccgggg gcttcaggat ctgggagcta tgaaaagtgt ctgcccaggc
      301 acttccggtt tttcttcccc caatccctct gctgcttctg ctgctgctca ggaggtcaga
      361 tctgccactg atggtaatac cagcaccact ccgcccacct ctgccaagaa gagaaagtta
      421 aacagcagca gtagcagcag taacagtagt aacgagagag aagactttga ttccacctct
      481 tcctcttcca cccctccaca acccagagat tcggcgtccc cttcaacctc gtccttctgc
      541 cttggggttc cagtggccac ttccagccac gtacctatac agaagaagct gcggtttgaa
      601 gacaccctgg agtttgtagg gattgatacg aaaatggctg aggaatcttc ctcctcatct
      661 tcctcatctt cgccaactgc tgcaacctca cagcagcagc agcagcaaca acttaaaact
      721 aagagtatat taatttcttc tgtggcttca gtgcaccatg caaacggcct ggctaaatct
      781 tctaccgcgg tctctagctt tgctaacagc aagcccggct cagctaagaa gttagtgatc
      841 aagaacttta aagataagcc taaattacca gaaaactata cggatgagac atggcaaaag
      901 ctaaaagaag cagtggaagc cattcagaat agtacttcaa ttaagtacaa tttagaagaa
      961 ctctaccagg ctgtagaaaa tctttgttct cataagattt ctgcaaattt gtataagcag
     1021 ctgaggcaga tatgtgaaga tcacatcaaa gcacagattc atcagttcag agaggattca
     1081 ttggatagtg ttctttttct caaaaagatc gatagatgct ggcaaaacca ctgtaggcaa
     1141 atgataatga tcaggagcat ttttttgttt ctggatagaa cttatgttct tcagaattca
     1201 atgctaccct ccatttggga catgggatta gagttattta gggctcatat tataagtgat
     1261 caaaaagtac agacaaaaac gattgatggc atccttctct tgattgagag agagcggaat
     1321 ggtgaagcaa ttgatagaag tttacttcga agccttttaa gtatgctgtc tgatttgcaa
     1381 atttaccaag attcttttga acagcaattt ttgcaagaaa ctaatcggct ttacgcagcc
     1441 gaaggtcaga agttaatgca agaaagagag gttcctgagt atcttcatca tgttaataaa
     1501 cgcctagaag aagaagctga cagacttatt acttacttag atcagaccac ccagaagtca
     1561 ctaattgctt ctgttgaaaa acaacttcta ggtgaacact taacggcaat tcttcaaaaa
     1621 ggtttaaata gcctccttga tgaaaaccga attcaagatt tgtctctcct gtatcagctc
     1681 ttcagtagag ttcggggtgg agttcaggtt ctcttacagc agtggattga gtatatcaag
     1741 gcttttggaa gtaccattgt tattaatcct gaaaaagata aaactatggt tcaagaatta
     1801 ttggacttta aagataaagt tgaccatata attgatacct gctttctgaa aaatgaaaaa
     1861 tttatcaatg ccatgaaaga agcttttgaa acattcatta ataaaagacc aaataagcca
     1921 gctgaactga tagctaagta tgtggattca aaacttcgtg caggcaacaa agaagctaca
     1981 gatgaagagc ttgagaaaat gttggataaa ataatgatta tatttagatt tatctatggt
     2041 aaagatgttt ttgaggcctt ctataagaaa gatttagcca aacgcctatt agttggcaag
     2101 agtgcatcag tagatgctga aaaatcaatg ctgtccaaac tgaaacacga atgtggagcc
     2161 gcttttacca gcaagcttga aggaatgttc aaagacatgg agctttctaa agatatcatg
     2221 attcagttta aacagtatat gcagaaccag aatgtacctg gcaatattga attaactgtt
     2281 aatatcctga caatgggtta ctggccaaca tatgtgccta tggaagtcca tttgccgcca
     2341 gagatggtaa agcttcagga aattttcaag acattttact taggcaagca cagtggcagg
     2401 aaactccagt ggcagtcaac cttgggacac tgtgtgctaa aagctgaatt taaagagggc
     2461 aaaaaagaac ttcaggtctc tctttttcaa accatggtgc tgctaatgtt taatgaagga
     2521 gaagaattca gtttagaaga gatcaagcat gctactggga tagaggatgg agaattaaga
     2581 agaacactgc agtcactagc ctgtggcaaa gctagagttc tggctaaaaa tcctaagggc
     2641 aaagatattg aagatggtga caaatttatt tgtaatgacg atttcaaaca caaactattc
     2701 aggataaaga tcaatcaaat ccagatgaaa gaaacggttg aagaacaagc aagcactaca
     2761 gaaagagtat ttcaagacag acagtaccaa attgatgctg cgatcgttcg aattatgaag
     2821 atgagaaaaa cacttagcca caatcttctt gtttcagaag tctacaatca gctaaaattt
     2881 ccagttaaac ctgctgatct taagaagaga atagaatccc tcattgaccg ggactacatg
     2941 gaaagagata aagaaaatcc aaatcagtac aactatattg catagaatgt tggccttgga
     3001 acttgtcata atgctgtagc cattggataa actaatttgt tgaatctcat gttatttgac
     3061 ttcttttata cttctgatga ccaaatgaag cagtgtaatg ggaatagttg tgtggacttt
     3121 tctcagtggt taacataccc attttaaaga gtaatactta acttttaaga agaatgttgt
     3181 tgaattctat gcatgttatt tagtatgatg tgcagaaaac tctttaagaa agtacctggg
     3241 tcttcatgat tccttttgga actggggaag agatccctat ggccattaaa aggggggagg
     3301 gtgattctta tacatcatta ataaagcaaa aggtttattt gcctaaaagt catttaaaga
     3361 tacttaaact gcatgcaaat tttatttact gtacagagtt ctggatgtat ttatcaaata
     3421 gaaaaaccac ccttgacttg gttgtttacc cattttgtgg tttcccttga aaagaaaagt
     3481 aagttttcat tgttgttggc attctgttac actgaagggg aaataatctt atttggaaac
     3541 aagtttttca agtagggtga cagttccttc catcgtacct tatatattcg catcctaaag
     3601 gtttcccatt aggactctgg gatgtgtaca taagacttag ccccacattc actgtgctga
     3661 ttaacaggaa cttattaaaa tagaaaattt gctgatcgtc ttgtactaca agaacatagc
     3721 aagaggggaa aacccaggtc cttttattaa aactgaatac agtatgaaaa tcacaggaga
     3781 ccaaaatcaa caaataagtt ttataagttg caggagtaaa aatgtaccag ctgcttttca
     3841 ttatgtttca gtggttaaat gtttgggtcg tgctgccgtc tttaagttgg gtggatcgtc
     3901 aattgtcaga ggtttcctag gatttgcatt ccagtttttt ctactaaatg attttttttt
     3961 ttactgtcat gcgcctcctg atttggagaa actcaacata tgattctccc ttgtcttcct
     4021 tgcacactcc ctattgaaaa cttcaagttc tgtagtcctt ttttttttaa ttgcttgaat
     4081 ttaagagttc tttatagaca ttggacactt gctgttcagg aagtgtctcc aaattggtct
     4141 cacacagaaa gtaaggaaag tattgttttt caatttcaaa ctgtgagtaa aagacttttc
     4201 ttcctcacca gaggactctg ctttgcttcc tggtttctct ttccatggag gaaatggatg
     4261 atggcaaaga agcagactgt ttttgtgttt tcttttacat tagaacttct tcagtgcttt
     4321 ctccactagc acatatcttt gtctttatgg agcaattagg catactttca gctccgaaga
     4381 cttgggggct cttatgtctg ctattgtttt cttgaattct caaagacctg tggctagcta
     4441 tctgtatttt ttaagttgcc tgtttctctc taatgcatca acttgcacac tgtttgacta
     4501 tgtacagtat ttagagttaa cttcccggtt ctttctgagc agctatttcg tatatttttt
     4561 ccagctcctc cctgatttcc ttacaggagc tcttctgaca aggaatgtgg ctgttatgtt
     4621 tactggatca tcaacatcag tgttgtaatt catccctgtg ttggtctctg ggatatggag
     4681 actattacac cactcactag ttactcttaa aagaatttgt aggtaggttt gttcctacta
     4741 ggacgctctt gataccacct tcattatctt gaaagtgggt gcctgtcctg aagttcttaa
     4801 ttctttaaaa acttgacggt gtttcagctg aaaagctagc cagactacta agggaaactg
     4861 tttatgaaac tgacaagtaa tggcaaactc cattggatta ttatgagttt cagttgttct
     4921 gtatcggttg aatttttgtg ctcttccctg tgtatgtgtg gtggttctat ttttctccaa
     4981 taaaactttt attttaaaaa aaaaaaaaaa aaaaaaaaaa
//
