LOCUS       NM_146207               3726 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus cullin 4A (Cul4a), transcript variant 1, mRNA.
ACCESSION   NM_146207
VERSION     NM_146207.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Zhang,Y., Zhou,H., Chen,X., Wang,N., Zhan,Y., Huang,Z., Ruan,K.,
            Qi,Q., Deng,M. and Jiang,Y.
  TITLE     A novel tRNA-derived fragment tRF-3023b suppresses inflammation in
            RAW264.7 cells by targeting Cul4a through NF-kappaB signaling
  JOURNAL   Funct Integr Genomics 24 (1), 9 (2024)
   PUBMED   38221594
  REMARK    GeneRIF: A novel tRNA-derived fragment tRF-3023b suppresses
            inflammation in RAW264.7 cells by targeting Cul4a through NF-kappaB
            signaling.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3726)
  AUTHORS   Hu,Y., Zhang,R., Lu,S., Zhang,W., Wang,D., Ge,Y., Jiang,F., Qin,X.
            and Liu,Y.
  TITLE     S100 Calcium Binding Protein A16 Promotes Cell Proliferation by
            triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to
            inhibit Hippo pathway in Glioma development
  JOURNAL   Int J Biol Sci 19 (7), 2034-2052 (2023)
   PUBMED   37151881
  REMARK    GeneRIF: S100 Calcium Binding Protein A16 Promotes Cell
            Proliferation by triggering LATS1 ubiquitin degradation mediated by
            CUL4A ligase to inhibit Hippo pathway in Glioma development.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3726)
  AUTHORS   Yin,Y., Zhu,L., Li,Q., Zhou,P. and Ma,L.
  TITLE     Cullin4 E3 Ubiquitin Ligases Regulate Male Gonocyte Migration,
            Proliferation and Blood-Testis Barrier Homeostasis
  JOURNAL   Cells 10 (10), 2732 (2021)
   PUBMED   34685710
  REMARK    GeneRIF: Cullin4 E3 Ubiquitin Ligases Regulate Male Gonocyte
            Migration, Proliferation and Blood-Testis Barrier Homeostasis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3726)
  AUTHORS   Yang,L., Chen,W., Li,L., Xiao,Y., Fan,S., Zhang,Q., Xia,T., Li,M.,
            Hong,Y., Zhao,T., Li,Q., Liu,W.H. and Xiao,N.
  TITLE     Ddb1 Is Essential for the Expansion of CD4+ Helper T Cells by
            Regulating Cell Cycle Progression and Cell Death
  JOURNAL   Front Immunol 12, 722273 (2021)
   PUBMED   34526995
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3726)
  AUTHORS   Li,B., Jia,N., Waning,D.L., Yang,F.C., Haneline,L.S. and Chun,K.T.
  TITLE     Cul4A is required for hematopoietic stem-cell engraftment and
            self-renewal
  JOURNAL   Blood 110 (7), 2704-2707 (2007)
   PUBMED   17616641
  REMARK    GeneRIF: Cul4A(+/-) hematopoietic stem-cells exhibit defects in
            engraftment and self-renewal capacity
REFERENCE   7  (bases 1 to 3726)
  AUTHORS   Li,B., Jia,N., Kapur,R. and Chun,K.T.
  TITLE     Cul4A targets p27 for degradation and regulates proliferation, cell
            cycle exit, and differentiation during erythropoiesis
  JOURNAL   Blood 107 (11), 4291-4299 (2006)
   PUBMED   16467204
  REMARK    GeneRIF: a Cul4A ubiquitin ligase positively regulates
            proliferation by targeting p27 for degradation and that Cul4A
            down-regulation during terminal erythroid differentiation allows
            p27 to accumulate and signal cell cycle exit
REFERENCE   8  (bases 1 to 3726)
  AUTHORS   Li,B., Yang,F.C., Clapp,D.W. and Chun,K.T.
  TITLE     Enforced expression of CUL-4A interferes with granulocytic
            differentiation and exit from the cell cycle
  JOURNAL   Blood 101 (5), 1769-1776 (2003)
   PUBMED   12393421
  REMARK    GeneRIF: Enforced CUL-4A expression does not alter the cell cycle
            distribution of uninduced cells. It increases the proportion of
            induced cells in S-phase & reduces the proportion in G0/G1. This
            CUL-4A regulatory function is interconnected with differentiation.
REFERENCE   9  (bases 1 to 3726)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 3726)
  AUTHORS   Li,B., Ruiz,J.C. and Chun,K.T.
  TITLE     CUL-4A is critical for early embryonic development
  JOURNAL   Mol Cell Biol 22 (14), 4997-5005 (2002)
   PUBMED   12077329
  REMARK    GeneRIF: CUL-4A is critical for early embryonic development.
            CUL-4A(-/-) embryos die between 4.5 and 7.5 dpc.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC133520.4.
            
            On May 18, 2018 this sequence version replaced NM_146207.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK170722.1, AK168215.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-281               AC133520.4         146871-147151       c
            282-397             AC133520.4         146465-146580       c
            398-501             AC133520.4         137201-137304       c
            502-571             AC133520.4         131028-131097       c
            572-645             AC133520.4         129894-129967       c
            646-808             AC133520.4         129145-129307       c
            809-898             AC133520.4         128815-128904       c
            899-981             AC133520.4         127901-127983       c
            982-1049            AC133520.4         124997-125064       c
            1050-1168           AC133520.4         119533-119651       c
            1169-1361           AC133520.4         118933-119125       c
            1362-1466           AC133520.4         116805-116909       c
            1467-1577           AC133520.4         116531-116641       c
            1578-1663           AC133520.4         116353-116438       c
            1664-1771           AC133520.4         115871-115978       c
            1772-1885           AC133520.4         112458-112571       c
            1886-1991           AC133520.4         110176-110281       c
            1992-2164           AC133520.4         109640-109812       c
            2165-2317           AC133520.4         108345-108497       c
            2318-3726           AC133520.4         104834-106242       c
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 5.73 cM"
     gene            1..3726
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /note="cullin 4A"
                     /db_xref="GeneID:99375"
                     /db_xref="MGI:MGI:1914487"
     exon            1..281
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             134..2413
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     CUL-4A"
                     /codon_start=1
                     /product="cullin-4A isoform 1"
                     /protein_id="NP_666319.2"
                     /db_xref="CCDS:CCDS52485.1"
                     /db_xref="GeneID:99375"
                     /db_xref="MGI:MGI:1914487"
                     /translation="MADEGPRKGSVSALMGRTNGLTKPAALAGGPAKPGGTGGSRKLV
                     IKNFRDRPRLPDNYTQDTWRKLHEAVKAIQSSTSIRYNLEELYQAVENLCSHKVSPTL
                     YKQLRQVCEDHVQAQILPFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTY
                     VLQNSMLPSIWDMGLELFRNHIISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLL
                     SMLSDLQVYKDSFELKFLEETNCLYAAEGQRLMQDREVPEYLNHVSKRLEEEADRVIT
                     YLDHSTQKPLIACVEKQLLGEHLTAILQKGLEHLLDENRVPDLTQMYQLFSRVKGGQH
                     ALLQHWSEYIKTFGTTIVINPEKDKDMVQDLLDFKDKVDHVVEVCFQRNERFINLMKE
                     SFETFINKRPNKPAELIAKHVDSKLRAGNKEATDEELERILDKIMILFRFIHGKDVFE
                     AFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMVHF
                     KQHMQNQSAPGPIDLTVNILTMGYWPTYTPMEVHLPPEMVRLQEVFKTFYLGKHSGRK
                     LQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLMFNEGDGFSFEEIKMATGIEDSEL
                     RRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNADFKHKLFRIKINQIQMKETVEEQV
                     STTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYNQLKFPVKPGDLKKRIESLI
                     DRDYMERDKDSPNQYHYVA"
     exon            282..397
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            398..501
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            502..571
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            572..645
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            646..808
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            809..898
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            899..981
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1049
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1050..1168
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1361
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1362..1466
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1577
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1578..1663
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1771
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1772..1885
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1991
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1992..2164
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2165..2317
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..3726
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3699..3704
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3726
                     /gene="Cul4a"
                     /gene_synonym="2810470J21Rik"
                     /note="major polyA site"
ORIGIN      
        1 agaaagagga ggagccgagc gctcacgggc ggagcccgac agcagcagga ggaggagccg
       61 ggcgcgcacg gagcgggcgc agggcaggag gaggagccgg gcggcgcggg gcggggcggc
      121 gtccggccca gccatggcgg acgagggccc tcggaagggc agcgtctcgg ctctcatggg
      181 ccgcaccaac ggcctcacca agcccgcggc gttggccggt ggccccgcca agccgggggg
      241 cacgggaggc tccaggaagc tggtcatcaa gaacttccga gacaggccgc ggctgcctga
      301 caactacact caggacacgt ggcggaagct tcacgaagcg gtcaaggcca tccagagcag
      361 cacatccatc aggtacaacc tggaggagct gtaccaggct gttgaaaatc tttgttctca
      421 caaagtctcc ccaacgctct acaagcagct gcgccaggtc tgtgaagacc atgtccaggc
      481 ccagatcctc ccattcagag aagactcact agacagcgtt ttatttctaa agaagattaa
      541 cacatgctgg caagatcact gcagacaaat gatcatgatc agaagcatct tcctgtttct
      601 ggatcgaacc tacgtcctcc agaattccat gctgccttct atctgggaca tgggcctaga
      661 gctgtttagg aaccacatca tcagcgacag gatggtgcag agtaagacca ttgatgggat
      721 cctgctgctg attgggcgtg aacgaagtgg agaggctgtg gacaggagtc tgcttcggag
      781 tttgctaagc atgctctcag atctccaggt gtacaaagac tcatttgagc tgaagttctt
      841 ggaagagact aactgtctat acgctgctga aggccagagg ctgatgcagg acagggaggt
      901 tccagaatac cttaaccatg tgagcaaacg tctagaagag gaagcagacc gagtgatcac
      961 ctacttagac cacagcacac aaaaaccgtt gattgcctgt gtggagaaac agctgttagg
     1021 agaacatttg acagcaattc tacagaaagg cctagagcac ctgctggatg agaacagggt
     1081 gcccgacctc acccagatgt accagctctt cagccgggtg aaaggagggc agcatgcgct
     1141 gctgcagcac tggagcgagt acatcaagac ctttggaaca accattgtca tcaatcctga
     1201 gaaagacaaa gacatggtcc aagacctgct ggactttaag gacaaagtgg accacgtggt
     1261 ggaggtgtgc ttccagagga acgagcgctt catcaacctg atgaaggagt ccttcgagac
     1321 gttcatcaac aagagaccaa acaagcctgc agagctcatt gccaagcacg tggactcaaa
     1381 gttacgggcc ggcaacaaag aagccacaga tgaggaactg gagaggatcc tggacaagat
     1441 catgattctc ttccgtttca tccatggtaa agatgtcttt gaagcgtttt ataagaaaga
     1501 cttagcaaaa aggctgctgg tggggaagag cgcgtcggtg gacgcggaga agtccatgct
     1561 gtcgaagctc aagcacgagt gcggagctgc gtttaccagc aaactggagg gcatgttcaa
     1621 ggacatggag ctctccaaag acatcatggt tcacttcaag cagcatatgc agaaccagag
     1681 tgcgccaggc cccattgacc tcacggtgaa catactcacc atgggctact ggcccacgta
     1741 cacacccatg gaagtgcacc tccctccaga gatggtcaga cttcaggaag tattcaagac
     1801 gttctacctg ggcaagcaca gtgggcggaa gctgcaatgg cagacaacgc tgggacacgc
     1861 cgtgctgaag gcggacttta aggaggggaa gaaggagttc caggtatccc tcttccagac
     1921 gctggtgctg ctcatgttca acgaagggga tggatttagc tttgaagaaa taaaaatggc
     1981 cactggcata gaggacagtg agttgcgaag aacactacag tctctggcct gtggtaaagc
     2041 acgggtccta attaaaagtc ccaaaggaaa ggaggtggaa gatggagata agttcatctt
     2101 caatgcagac ttcaaacaca agttgttcag aataaagatc aatcaaatcc agatgaagga
     2161 gaccgttgaa gaacaggtta gcactacaga gagagtattc caggatagac agtaccagat
     2221 cgacgctgct atagtcagaa taatgaaaat gaggaagaca cttggtcata atcttctagt
     2281 ttctgaatta tacaatcagc tgaaatttcc agtgaagcct ggagatttga aaaaaaggat
     2341 tgagtcactt atagacagag actatatgga acgagacaaa gacagtccaa atcagtacca
     2401 ctacgtggca tgacgcacca gctgactgtc gtctcccagt gagacacaca cacgaagcac
     2461 ctgagagtga agctgctgtg ccgtctccag gtccctgcag ctgccccgac tgtggacgct
     2521 tggattcgga gcagaggatg tggttcttgg ctcatccttc ccagggcgca agggctttga
     2581 gatgacaccc atcttccctc cctgcttctt cccctacttc tttattgttt ctgttactct
     2641 ctaacttcgc attgagaagt tccttcacaa gctctcgttc ctctggcaga aagccacggg
     2701 cttggtttgt tgtgtgtgtg attgtgggac acagtggaat taccctagct gctgtgttct
     2761 tcccctcatg aagactcaga gagaaagaga gagccctggg ctggggcact ggggagaaac
     2821 tgtccaggct gccactcgga tctctgcacc ctgagcattg gcctctcttc tgtgtatttt
     2881 cagaaagttg gaaactttgg cttctgtaaa tcaggtttct aaatgaggtc cctgatgtgc
     2941 caagaggaca tcatgtctgt catgagacac agctcaccca gagggctgct tgtcgtttgt
     3001 tgaggggctt gggggtttca tgtttgtttc catgctggtt gagtgtggag gtcctggttt
     3061 tggctttggg gtgactgctg tttgactgtt gaagttgcag tttgtgtgat taccataata
     3121 aaatcgggcc tggttttata gtcttgcaca gagaccgtct tggatctctg ccctggcact
     3181 atatggagtt tagttatgtt tgtcaaaagg caaaggaatt gttggttcct ggagacatac
     3241 tgtgtgtaca ggtgggttgg gcagcgagaa cgaggctcca tagtggtgtt ggcatggtga
     3301 tgatgacaga cacttttgca acatttaaaa gtactttata ttttacaaag tagcatgttt
     3361 cattttgatt aaaagctacc aaaggaattt tgatcatgta taagtgttta aagcaatatt
     3421 ttctggaata taccaagttt atataactcg attttatgct aaattattaa aggattctcc
     3481 ctttttgaaa catgcatgtt taagatacaa catctttttg ggcccccata ttaaaaccct
     3541 ttctctttaa gtatcatttc tatatttgta cattttcatt tatgagttct atgatgtggt
     3601 ctctgaaaga ccaaatagat ttctatttct acttcttatt tttaagttca ttgtgtctac
     3661 aaattgttaa tattgtaatt taatgtagac ttactttgaa taaaactagt ttaattggcc
     3721 tttaaa
//
