LOCUS       NM_153775               3066 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cytosolic thiouridylase subunit 2 (Ctu2), transcript
            variant 1, mRNA.
ACCESSION   NM_153775
VERSION     NM_153775.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Schlieker,C.D., Van der Veen,A.G., Damon,J.R., Spooner,E. and
            Ploegh,H.L.
  TITLE     A functional proteomics approach links the ubiquitin-related
            modifier Urm1 to a tRNA modification pathway
  JOURNAL   Proc Natl Acad Sci U S A 105 (47), 18255-18260 (2008)
   PUBMED   19017811
  REMARK    GeneRIF: Identifies an homologous human protein that plays a role
            in tRNA modification.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ067802.1, AK090391.1, AK090349.1 and AC114917.2.
            
            On Mar 28, 2009 this sequence version replaced NM_153775.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036332.1, AK154995.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-66                CJ067802.1         2-67
            67-602              AK090391.1         2-537
            603-919             AK090391.1         539-855
            920-1894            AK090349.1         516-1490
            1895-3066           AC114917.2         80889-82060
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 71.26 cM"
     gene            1..3066
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /note="cytosolic thiouridylase subunit 2"
                     /db_xref="GeneID:66965"
                     /db_xref="MGI:MGI:1914215"
     exon            1..153
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..1630
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /note="cytosolic thiouridylase subunit 2 homolog"
                     /codon_start=1
                     /product="cytoplasmic tRNA 2-thiolation protein 2"
                     /protein_id="NP_722470.2"
                     /db_xref="CCDS:CCDS52694.1"
                     /db_xref="GeneID:66965"
                     /db_xref="MGI:MGI:1914215"
                     /translation="MCQAGEDYAGPARREPPPVPRPSREQKCVKCAEGLPVVVIRAGD
                     AFCRVCFKAFYVHKFRAMLGKNRVIFPGEKVLLSWSGGPSSSSMVWQVLEGLSQDSAK
                     RLRFVPGVIYVDEGAACGQSLEDRQKTVAEVKRILENTGFPWHVVALEEVFSLPPSVL
                     CCTSQESAGTEEAYKAAVDRFLQQQQQQQQRVLGAEAGASPAQGEARLHPSHGREPSG
                     TAGYPTAAQTEALSRLFSSIKTLTAKEELLQTLRTHLIVHIARVHGYCKVMTGETCTR
                     LAIKLMTNLALGRGAFLAWDTGFSDERHGDVVLVRPMRDHTLKEVAFYNHLFRVPSVF
                     TPAIDTKAPEKASIHRLMEAFILRLQTLFPSTVSTVYRTSEKLVKAPREGCAAGPSGP
                     SCLLCMCALDIDTADSATAFGAQSSSHLSQMPSAEAGMPTQPCCAAGEGQAQSCHREV
                     GKRGDARACITEQLCYSCRVNMKDLPSLDPLPPYVLAEAQLRSQRGSVSEEIQEYLIT
                     DEEEDS"
     exon            154..228
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            229..307
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            308..367
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            368..428
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            429..538
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            539..840
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            841..976
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1108
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1200
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1304
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1443
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1510
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1569
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     exon            1570..3066
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1698..1703
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /note="hexamer: AATAAA"
     polyA_site      1721
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /note="major polyA site"
ORIGIN      
        1 cgctcggcgg tgacggcatc tcagcgcgcc ggcatctcag cgcgccggct cctcacgcca
       61 ctccgcagtc gggacccacc cctccatgtg tcaggccggt gaggattacg cggggccggc
      121 gcggcgggag cctccgcccg tgccgcggcc aagccgggag cagaaatgtg tgaagtgcgc
      181 agaaggcctg ccggtggtgg tgatacgagc gggagatgcc ttctgcaggg tttgtttcaa
      241 ggcattctac gttcacaaat tcagagccat gcttgggaag aaccgggtca tctttcctgg
      301 ggagaaggtg ctcctgtcct ggtctggggg cccttcgtcc agctccatgg tctggcaagt
      361 gcttgagggc ctgagtcagg attctgcgaa acggctgcgc tttgtgccag gagtcatcta
      421 tgttgatgag ggcgcagcct gtggtcagag cttggaggac agacagaaga cggtggctga
      481 ggtgaagcgg atcctggaga acactggttt cccatggcat gtggttgcct tagaagaggt
      541 gttcagcttg cccccatcag tgctatgctg cacttcccag gagtcagcag ggacagagga
      601 ggcctacaag gctgccgtgg accgcttcct gcagcagcag cagcagcagc agcagcgtgt
      661 gctgggagct gaggccggtg ccagcccagc tcagggagag gctcggctgc atccatccca
      721 cggccgggaa ccctcaggca cggccgggta ccccacagct gctcagacag aggccttgtc
      781 taggttgttc agctccataa agacactgac agccaaagag gagcttctgc agaccctgag
      841 gacccatctg attgtgcaca tagcccgagt ccatggctat tgcaaggtaa tgacagggga
      901 gacctgcacc cgcctggcca tcaagcttat gaccaacctg gccctgggga gaggcgcctt
      961 cctcgcctgg gacacgggct tctcagatga gcgacacggg gatgtggtgt tggtgaggcc
     1021 catgcgggac cacacgctga aggaagtggc cttctacaac cacctgttta gagtcccctc
     1081 agtcttcaca ccagccatcg ataccaaggc tccagaaaag gctagcatcc accggctgat
     1141 ggaggccttt atcctgaggc tacagactct gttcccgtct acagtcagca ccgtgtacag
     1201 gacgagtgag aagctggtca aagctccccg ggagggttgt gccgctggcc cgtcaggccc
     1261 cagctgcctc ctgtgcatgt gtgccctgga cattgacact gctgacagtg ccacggcctt
     1321 tggggctcag tcctcctcac atctctccca gatgccgagt gctgaggctg ggatgcctac
     1381 ccagccctgc tgtgctgcag gggagggtca ggcccagagc tgccataggg aagttggcaa
     1441 gaggggggat gcacgggcct gcatcacaga gcagttatgt tatagctgcc gagtgaacat
     1501 gaaggacctg ccctccttgg acccattgcc accctacgtc cttgctgagg ctcagctccg
     1561 cagccagagg ggctcggtct ctgaggaaat ccaggagtat ctgattacag atgaggagga
     1621 agacagctga gtcgtgcgga gccatgaagc aggaaggcga ggacaaaagg attggtctgt
     1681 gacagtccgt ttgtataaat aaaaatgttt tacttagaaa accctacagt acttgtggga
     1741 agggagagcc aggcccccag accaactggc agggacgcaa tgggtaggga cacagcgtcc
     1801 ctttgacagc agcatcatca gggcttacgt aggccaggac ctgtcacagc tcgtgccttg
     1861 ggcagctaca ctccaaccca aggagctctg ccagccctgg tggtcatgct gccctgaggc
     1921 agctccttca ttcccggtgc ccagggcctg ggctcctact ccctctcacg tgtccactta
     1981 atcatggtct ctggagatcg gtacaggaag atgagcttgg cgtatagctc ctcctccagc
     2041 tccagctccc gggtctcgcg caccaagaag atgtcctggc acagcttgag gatgcggtcc
     2101 acacacggca gttcctcgaa catgatggag tgagagatct cgctgaagaa gccccgcaca
     2161 aacttgccaa ccaccagcac gatggagacg tacagcccca caatcctgtc gggagaggcg
     2221 ggcatgtgag ccgggaccac tcagaaccct tggggctctc ggagatccct ctgcagtact
     2281 cacccgtagc cggccaggaa gcccaggcta ggtgggctga ccttgtcact gaagatgacc
     2341 atgggcagca ggttgcagtc agccttgcag tcctgcagct cgatgaccca ccactcgagg
     2401 aagtcggagg ccttggtgcc cgcttgctcc ccagaggccc ctgtgcccac ttgctccctc
     2461 cgcagctgga tgcgcacacc aaggtagtcc tcttcctcat ctgagttgag tgggatagtt
     2521 agagtcgggc tacactggcc cccggcctag cccgcatccc tacatccctg cacccacact
     2581 tccggcctag cccacatccc tgcgccctgc tcccacactg acctggctgc agctgcttca
     2641 cagggttggc ttcaggccca ttgggagcac gaatgtactt ggggaagaga tggggaatga
     2701 ccctgtggga ggagtcagca agtcaggggc atcttagccc tcctgcccac atgccccaca
     2761 gtggccccca ctcacactga ctgctcaggt caggggcatc ttagccctcc tgcccacatg
     2821 ccccacagtg gcccccactc acactgactg gtcaggtctg ccctcgagca gttgggccag
     2881 ctgccttcgt gccgtactgt tgggggccag ctccaaggtg tgcttctcat tagtatactc
     2941 cacagtgcca cccttggcca ggtccctggg gagcaggcag tgttaggcag tgtggggcat
     3001 cccccagacc ctcataagaa caggacccac ctttggaaat tccaggtaaa gcgcagtgta
     3061 atgtcg
//
