LOCUS       NP_722470                514 aa            linear   ROD 30-APR-2024
DEFINITION  cytoplasmic tRNA 2-thiolation protein 2 [Mus musculus].
ACCESSION   NP_722470
VERSION     NP_722470.2
DBSOURCE    REFSEQ: accession NM_153775.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 514)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 514)
  AUTHORS   Schlieker,C.D., Van der Veen,A.G., Damon,J.R., Spooner,E. and
            Ploegh,H.L.
  TITLE     A functional proteomics approach links the ubiquitin-related
            modifier Urm1 to a tRNA modification pathway
  JOURNAL   Proc Natl Acad Sci U S A 105 (47), 18255-18260 (2008)
   PUBMED   19017811
  REMARK    GeneRIF: Identifies an homologous human protein that plays a role
            in tRNA modification.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ067802.1, AK090391.1, AK090349.1 and AC114917.2.
            
            On Mar 28, 2009 this sequence version replaced NP_722470.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036332.1, AK154995.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 71.26 cM"
     Protein         1..514
                     /product="cytoplasmic tRNA 2-thiolation protein 2"
                     /note="cytosolic thiouridylase subunit 2 homolog"
                     /calculated_mol_wt=55974
     Region          1..23
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q3U308.1)"
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylcysteine.
                     /evidence=ECO:0000250|UniProtKB:Q2VPK5; propagated from
                     UniProtKB/Swiss-Prot (Q3U308.1)"
     Region          192..222
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q3U308.1)"
     Region          <250..380
                     /region_name="TilS"
                     /note="tRNA(Ile)-lysidine synthase TilS/MesJ [Translation,
                     ribosomal structure and biogenesis]; COG0037"
                     /db_xref="CDD:439807"
     Region          347..470
                     /region_name="CTU2"
                     /note="Cytoplasmic tRNA 2-thiolation protein 2; pfam10288"
                     /db_xref="CDD:463044"
     Site            421
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q2VPK5; propagated from
                     UniProtKB/Swiss-Prot (Q3U308.1)"
     Site            425
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q2VPK5; propagated from
                     UniProtKB/Swiss-Prot (Q3U308.1)"
     CDS             1..514
                     /gene="Ctu2"
                     /gene_synonym="2310061F22Rik; C16orf84; Ncs2"
                     /coded_by="NM_153775.2:86..1630"
                     /db_xref="CCDS:CCDS52694.1"
                     /db_xref="GeneID:66965"
                     /db_xref="MGI:MGI:1914215"
ORIGIN      
        1 mcqagedyag parrepppvp rpsreqkcvk caeglpvvvi ragdafcrvc fkafyvhkfr
       61 amlgknrvif pgekvllsws ggpssssmvw qvleglsqds akrlrfvpgv iyvdegaacg
      121 qsledrqktv aevkrilent gfpwhvvale evfslppsvl cctsqesagt eeaykaavdr
      181 flqqqqqqqq rvlgaeagas paqgearlhp shgrepsgta gyptaaqtea lsrlfssikt
      241 ltakeellqt lrthlivhia rvhgyckvmt getctrlaik lmtnlalgrg aflawdtgfs
      301 derhgdvvlv rpmrdhtlke vafynhlfrv psvftpaidt kapekasihr lmeafilrlq
      361 tlfpstvstv yrtseklvka pregcaagps gpscllcmca ldidtadsat afgaqssshl
      421 sqmpsaeagm ptqpccaage gqaqschrev gkrgdaraci teqlcyscrv nmkdlpsldp
      481 lppyvlaeaq lrsqrgsvse eiqeylitde eeds
//
