LOCUS       NM_007798               4756 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus cathepsin B (Ctsb), mRNA.
ACCESSION   NM_007798
VERSION     NM_007798.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Zierke,L., John,D., Gischke,M., Tran,Q.T., Sendler,M., Weiss,F.U.,
            Bornscheuer,U.T., Ritter,C., Lerch,M.M. and Aghdassi,A.A.
  TITLE     Initiation of acute pancreatitis in mice is independent of fusion
            between lysosomes and zymogen granules
  JOURNAL   Cell Mol Life Sci 81 (1), 207 (2024)
   PUBMED   38709385
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Lu,J., Li,H., Yu,Z., Cao,C., Xu,Z., Peng,L., Zhang,J.H. and Chen,G.
  TITLE     Cathepsin B as a key regulator of ferroptosis in microglia
            following intracerebral hemorrhage
  JOURNAL   Neurobiol Dis 194, 106468 (2024)
   PUBMED   38460801
  REMARK    GeneRIF: Cathepsin B as a key regulator of ferroptosis in microglia
            following intracerebral hemorrhage.
REFERENCE   3  (bases 1 to 4756)
  AUTHORS   Ha,H.T., Liu,S., Nguyen,X.T., Vo,L.K., Leong,N.C., Nguyen,D.T.,
            Balamurugan,S., Lim,P.Y., Wu,Y., Seong,E., Nguyen,T.Q., Oh,J.,
            Wenk,M.R., Cazenave-Gassiot,A., Yapici,Z., Ong,W.Y., Burmeister,M.
            and Nguyen,L.N.
  TITLE     Lack of SPNS1 results in accumulation of lysolipids and lysosomal
            storage disease in mouse models
  JOURNAL   JCI Insight 9 (8), e175462 (2024)
   PUBMED   38451736
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4756)
  AUTHORS   Li,H., Zhao,Q., Liu,D., Zhou,B., Liao,F. and Chen,L.
  TITLE     Cathepsin B aggravates atherosclerosis in ApoE-deficient mice by
            modulating vascular smooth muscle cell pyroptosis through NF-kappaB
            / NLRP3 signaling pathway
  JOURNAL   PLoS One 19 (1), e0294514 (2024)
   PUBMED   38165884
  REMARK    GeneRIF: Cathepsin B aggravates atherosclerosis in ApoE-deficient
            mice by modulating vascular smooth muscle cell pyroptosis through
            NF-kappaB / NLRP3 signaling pathway.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4756)
  AUTHORS   Hu,K., Park,Y., Olivas,Y., Chen,A., Liu,C. and Hu,B.
  TITLE     Cathepsin B knockout confers significant brain protection in the
            mouse model of stroke
  JOURNAL   Exp Neurol 368, 114499 (2023)
   PUBMED   37506756
  REMARK    GeneRIF: Cathepsin B knockout confers significant brain protection
            in the mouse model of stroke.
REFERENCE   6  (bases 1 to 4756)
  AUTHORS   Aggarwal,N. and Sloane,B.F.
  TITLE     Cathepsin B: multiple roles in cancer
  JOURNAL   Proteomics Clin Appl 8 (5-6), 427-437 (2014)
   PUBMED   24677670
  REMARK    Review article
REFERENCE   7  (bases 1 to 4756)
  AUTHORS   Qian,F., Chan,S.J., Achkar,C., Steiner,D.F. and Frankfater,A.
  TITLE     Transcriptional regulation of cathepsin B expression in B16
            melanomas of varying metastatic potential
  JOURNAL   Biochem Biophys Res Commun 202 (1), 429-436 (1994)
   PUBMED   8037744
REFERENCE   8  (bases 1 to 4756)
  AUTHORS   Qian,F., Frankfater,A., Chan,S.J. and Steiner,D.F.
  TITLE     The structure of the mouse cathepsin B gene and its putative
            promoter
  JOURNAL   DNA Cell Biol 10 (3), 159-168 (1991)
   PUBMED   2012677
REFERENCE   9  (bases 1 to 4756)
  AUTHORS   Ferrara,M., Wojcik,F., Rhaissi,H., Mordier,S., Roux,M.P. and
            Bechet,D.
  TITLE     Gene structure of mouse cathepsin B
  JOURNAL   FEBS Lett 273 (1-2), 195-199 (1990)
   PUBMED   2226854
REFERENCE   10 (bases 1 to 4756)
  AUTHORS   Chan,S.J., San Segundo,B., McCormick,M.B. and Steiner,D.F.
  TITLE     Nucleotide and predicted amino acid sequences of cloned human and
            mouse preprocathepsin B cDNAs
  JOURNAL   Proc Natl Acad Sci U S A 83 (20), 7721-7725 (1986)
   PUBMED   3463996
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK083393.1, AK151790.1,
            AC090654.38 and BI733634.1.
            
            On Nov 24, 2011 this sequence version replaced NM_007798.2.
            
            Summary: This gene encodes a member of the peptidase C1 family and
            preproprotein that is proteolytically processed to generate
            multiple protein products. These products include the cathepsin B
            light and heavy chains, which can dimerize to generate the double
            chain form of the enzyme. This enzyme is a lysosomal cysteine
            protease with both endopeptidase and exopeptidase activity that may
            play a role in protein turnover. Homozygous knockout mice for this
            gene exhibit reduced pancreatic damage following induced
            pancreatitis and reduced hepatocyte apoptosis in a model of liver
            injury. Pseudogenes of this gene have been identified in the
            genome. [provided by RefSeq, Aug 2015].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083393.1, AK151790.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1951              AK083393.1         1-1951
            1952-3202           AK151790.1         1898-3148
            3203-4264           AC090654.38        144685-145746
            4265-4756           BI733634.1         1-492
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 33.24 cM"
     gene            1..4756
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /note="cathepsin B"
                     /db_xref="GeneID:13030"
                     /db_xref="MGI:MGI:88561"
     exon            1..104
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     exon            105..255
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1149
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /EC_number="3.4.22.1"
                     /note="cathepsin B1"
                     /codon_start=1
                     /product="cathepsin B preproprotein"
                     /protein_id="NP_031824.1"
                     /db_xref="CCDS:CCDS27197.1"
                     /db_xref="GeneID:13030"
                     /db_xref="MGI:MGI:88561"
                     /translation="MWWSLILLSCLLALTSAHDKPSFHPLSDDLINYINKQNTTWQAG
                     RNFYNVDISYLKKLCGTVLGGPKLPGRVAFGEDIDLPETFDAREQWSNCPTIGQIRDQ
                     GSCGSCWAFGAVEAISDRTCIHTNGRVNVEVSAEDLLTCCGIQCGDGCNGGYPSGAWS
                     FWTKKGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPRCNKSCEAGYSPS
                     YKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMG
                     GHAIRILGWGVENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRTDQ
                     YWGRF"
     exon            256..341
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     exon            342..456
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     exon            457..575
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     exon            576..661
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     exon            662..805
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     exon            806..922
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1051
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..4743
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1930..1935
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /note="hexamer: AATAAA"
     polyA_site      1955
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /note="major polyA site"
     polyA_site      1992
                     /gene="Ctsb"
                     /gene_synonym="CB"
     regulatory      3548..3553
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /note="hexamer: AAGAAA"
     polyA_site      3564
                     /gene="Ctsb"
                     /gene_synonym="CB"
     regulatory      4721..4726
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ctsb"
                     /gene_synonym="CB"
                     /note="hexamer: ATTAAA"
     polyA_site      4740
                     /gene="Ctsb"
                     /gene_synonym="CB"
ORIGIN      
        1 agcggagggc ggagctgcgc gggtacttag gagtgcacgg gagaggccag gagcggctcc
       61 gtttggcttg gtggctgcag gcccaggctg tcgcgctgcg ctcggtgagt gcaggatcca
      121 gcatccagga tgtggtggtc cttgatcctt ctttcttgcc tgctggcact gaccagtgcc
      181 catgacaagc cttccttcca cccgctgtcg gatgacctga ttaactatat caacaaacag
      241 aatacaacat ggcaggctgg acgcaacttc tacaatgttg acataagcta tctgaagaag
      301 ctgtgtggca ctgtcctggg tggacccaaa ctgccaggaa gggttgcgtt cggtgaggac
      361 atagatctac ctgaaacctt tgatgcacgg gaacaatggt ccaactgccc gaccattgga
      421 cagattagag accagggctc ctgcggctct tgttgggcat ttggggcagt ggaagccatt
      481 tctgaccgaa cctgcattca caccaatggc cgagtcaacg tggaggtgtc tgctgaagac
      541 ctgcttactt gctgtggtat ccagtgtggg gacggctgta atggtggcta tccctctgga
      601 gcatggagct tctggacaaa aaaaggcctg gtttcaggtg gagtctacaa ttctcatgta
      661 ggctgcttac catacaccat ccctccctgc gagcaccatg tcaatggctc ccgtccccca
      721 tgcactggag aaggagatac tcccaggtgc aacaagagct gtgaagctgg ctactcccca
      781 tcctacaaag aggataagca ctttgggtac acttcctaca gcgtgtctaa cagtgtgaag
      841 gagatcatgg cagaaatcta caaaaatggc ccagtggagg gtgccttcac tgtgttttct
      901 gacttcttga cttacaaatc aggagtatac aagcatgaag ccggtgatat gatgggtggc
      961 cacgccatcc gcatcctggg ctggggagta gagaatggag ttccctactg gctggcagcc
     1021 aactcttgga accttgactg gggtgataat ggcttcttta aaatcctcag aggagagaac
     1081 cactgtggca ttgaatcaga aattgtggct ggaatcccac gcactgacca gtactgggga
     1141 agattctaat ctgctttgac ttcattgtcc agtccttagg ggctttttcc aaaatttagc
     1201 ggccctggca gggaatgagg tagacagggg agtctttgat tctttgagtt catctaacat
     1261 gcatgaagct tttggcaaga tttggacgac tggaccagag ctgcctgcca tcagagctac
     1321 cctccaaaca ctgtccttac ctggcctcct cctagcccat cccacgacag tgatcacagt
     1381 ctgcatccta gcctctccct agactagtgc ggtttgtagt gcctgctgtt gtggctctgc
     1441 ctgccatacc tcccaccccc atccccatcc cagcatccga ggacccaagg gctttgactg
     1501 caggacttcc aaaagaacga acacttcacg agaggacaaa tgccacctct cagctgcatc
     1561 ttgaagctgg tcacttctgg gtctgtggca ggcattcgtc aatggcccag tgcagttctc
     1621 tggagaaagc taccttttcc caaggcatct gcatccatcg acattggtaa tgtggccagc
     1681 tctccttggt cctgtcctca gccgatgctt tttcaatagg atttttatgc tttgtgtacc
     1741 tcaaccaagt atgaagagct gtactggttt tatagatcac ccccaattgt atggcttaaa
     1801 acaagctaca tggtcatggt ttgctgtgtg accgacagat aaccacaatg aaactagtct
     1861 ggaaaaaaaa acttgctttt tgttgtagtt accacttaac cctgtagttt aacaaggaat
     1921 gtctgtgcca ataaaccaat tctccctctg cttgacattg gcagggtgca cggttctgtg
     1981 ctgcagtttc aaggcttttg tctctccccc tggtttcctg tcaccctcag aaatggttct
     2041 caaaaccgta cctgtaactg ctcacacctc ggagcacttc ctcctgctta gagataaggg
     2101 agctacactt gtttaattaa ccaaggcggg cactcacata catatgaccc ggactgccat
     2161 cttcttagag gtcatttccc tggagtgggg gccaaagctt gcgcaccatg tttaagtttt
     2221 ctgggttact ggatttcatg gattttacaa aaatcatagt gaagtacagt tcaaagggaa
     2281 cagggagcct cactcatcag cgttgtccta tggatctgtt acttcctgct aagtgagagg
     2341 gaggggctgg tttctctgga agcccgcctt cccatgtcgg caatcagaac tatatcaaag
     2401 aagctctgga tgcctggcga gtgctagaag tgtaactcgg tttgaggcaa cctggcttgg
     2461 gtactggggc tttagtactg caagacagtt ccaccggtct caactacacc caatagttcc
     2521 ctcctacact tcctttcttg cctcaccaca tgcccacctg caactccctc cctacctcgc
     2581 atagccagtg aagatgagct aaattaagag aagaggaccc tgaacatcct ctgatggagc
     2641 ccaggaaacc cgttttgtta tcgtatgctt gggggtggct ctagatgtct tccataacca
     2701 gattctcctg tgggtggttt cctgtgctca agtcaagagc tgtcttcctt gggtacatga
     2761 aatggatact caggagacag aggcaagccg atctctggga gtttgaggcc agactggtct
     2821 acaatagacc ttgaagctag gagcatttcc ttttcagcct gcccacaggg ccttttgatg
     2881 attctgtgtt tacacatccc tacctttcta cacattagac tagaacacgc cgttgtgtgt
     2941 tctttgtgtg gtcctcaaac ctgagccagt atgagtgcca ggcctttgaa tagtcgtttg
     3001 tagcccgact aggtcatttc agatagattt gggcgatggc cttcaaacag ggcctatgac
     3061 agacagcatt catgctgccc aggaactcat taggtaacat gccagatcaa cttggtcaga
     3121 agtcttgttc cagatccatt agaatacaga actagcctta gcgctctcac ttccactacc
     3181 acagagcctg ggaaactcaa cagcaggggg aacgccttca aagggctgtc ccagacagga
     3241 tttgcaaata accagaggaa ggtagcaagc acatctttct aataattatg tctggatatg
     3301 ggtgggcctc tgagaaagca cacagatggt gtaaattata ttgagagtca aaagcaggct
     3361 agggatgtct ccatggtaga gtgtttgtcc agcatattgt ccaagcaaga cccttggttc
     3421 agtgcccagc aacacaataa caggagaaag aggtgtgtca tatgtagaca gtatgtataa
     3481 tgtccgacta aatgtatttc tagataatgg ctttatagaa atgcaccatg ctaaatatct
     3541 gaatataaag aaaactatct gaaattcaag tggaacttgg ggtcttattt gctaagggaa
     3601 ataagtttca gggggttaga gtctgtcccc cctacccctt tatatttatt catgcagttt
     3661 atgagtactc tgtcttcatg cacatcagaa ggaatcagat ctcattatag atggtagtga
     3721 gccactatgg ggttgctggg aattgaactt aggacctctg gaagaacact caggtgctga
     3781 gccatctctc cagcctccag gttttgatgt tagagacagt gtcttatgta gtccaggccc
     3841 acctccacaa ctgaggagga tcttaaaatc caactcctcc cttcctccct ccctccctcc
     3901 acctcccaca tgctgggatt actggtaaga gttgtagctt ccagaaagga tgaacattaa
     3961 aaccttcggt ttcagcgggg tggtatttgt tcaaggctta gagcaagtga ccaatgaagt
     4021 ctccatgccc cagtaatcca gcaaggaatc tgtgcttaca aagttgaatg gaccactgaa
     4081 ctggccgagg cacctcagtg atgaagtcaa catacgcagc aggcttaaga ggggagcaga
     4141 ctatgctcta catgtctctg tggtctgagc gtaccccaaa gggtcatata caggttcaca
     4201 aaatgacagg tagctgaggc actgttctca aaagggctca atgctagtct tccagagtta
     4261 gggctccaga gaccaagctc tgtggacaac agtggggtgg ctcgtgccta taactcgtac
     4321 attcagaagg ctgaggcagt gggatcacca ttaaattcaa ggctacatag acttgcaggc
     4381 caacctgagc tacagttaca gtgtacaagt gtcagcatgc cagggcgtcg actcctgctt
     4441 cctgtcacgt gctctctcct gcatgtcctc tctctactgt agtgtcattt ctcacaaggc
     4501 cgtccaaggg ctaaagatgg ggcccccctc atcttgtact ttcatcctcc taagctgtta
     4561 cataaacaat cctctttcct tggtaaagca gttggcctca ggtatcttgt cctacagtca
     4621 gagtggttgc ctagagcctc tccaccgctc aaacactggt tttcagatac acagggtgtc
     4681 agaggccacc atgacaatgt agccaactaa ccacgctgca attaaaaacc taccaatata
     4741 gaaaaaaaaa aaaaaa
//
