LOCUS       NM_008906               3301 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cathepsin A (Ctsa), transcript variant 1, mRNA.
ACCESSION   NM_008906
VERSION     NM_008906.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Park,S., Huang,H., Kwon,W., Kim,H.Y., Park,J.K., Han,J.E.,
            Cho,G.J., Han,S.H., Sung,Y., Ryoo,Z.Y., Kim,M.O. and Choi,S.K.
  TITLE     Cathepsin A regulates pluripotency, proliferation and
            differentiation in mouse embryonic stem cells
  JOURNAL   Cell Biochem Funct 39 (1), 67-76 (2021)
   PUBMED   32529664
  REMARK    GeneRIF: Cathepsin A regulates pluripotency, proliferation and
            differentiation in mouse embryonic stem cells.
REFERENCE   3  (bases 1 to 3301)
  AUTHORS   Hohl,M., Mayr,M., Lang,L., Nickel,A.G., Barallobre-Barreiro,J.,
            Yin,X., Speer,T., Selejan,S.R., Goettsch,C., Erb,K.,
            Fecher-Trost,C., Reil,J.C., Linz,B., Ruf,S., Hubschle,T., Maack,C.,
            Bohm,M., Sadowski,T. and Linz,D.
  TITLE     Cathepsin A contributes to left ventricular remodeling by degrading
            extracellular superoxide dismutase in mice
  JOURNAL   J Biol Chem 295 (36), 12605-12617 (2020)
   PUBMED   32647007
  REMARK    GeneRIF: Cathepsin A contributes to left ventricular remodeling by
            degrading extracellular superoxide dismutase in mice.
REFERENCE   4  (bases 1 to 3301)
  AUTHORS   Rottier,R.J., Hahn,C.N., Mann,L.W., del Pilar Martin,M.,
            Smeyne,R.J., Suzuki,K. and d'Azzo,A.
  TITLE     Lack of PPCA expression only partially coincides with lysosomal
            storage in galactosialidosis mice: indirect evidence for spatial
            requirement of the catalytic rather than the protective function of
            PPCA
  JOURNAL   Hum Mol Genet 7 (11), 1787-1794 (1998)
   PUBMED   9736781
REFERENCE   5  (bases 1 to 3301)
  AUTHORS   Rottier,R.J., Bonten,E. and d'Azzo,A.
  TITLE     A point mutation in the neu-1 locus causes the neuraminidase defect
            in the SM/J mouse
  JOURNAL   Hum Mol Genet 7 (2), 313-321 (1998)
   PUBMED   9425240
REFERENCE   6  (bases 1 to 3301)
  AUTHORS   Rottier,R.J. and D'Azzo,A.
  TITLE     Identification of the promoters for the human and murine protective
            protein/cathepsin A genes
  JOURNAL   DNA Cell Biol 16 (5), 599-610 (1997)
   PUBMED   9174165
REFERENCE   7  (bases 1 to 3301)
  AUTHORS   Zhou,X.Y., Morreau,H., Rottier,R., Davis,D., Bonten,E.,
            Gillemans,N., Wenger,D., Grosveld,F.G., Doherty,P., Suzuki,K.,
            Grosveld,G.C. and d'Azzo,A.
  TITLE     Mouse model for the lysosomal disorder galactosialidosis and
            correction of the phenotype with overexpressing erythroid precursor
            cells
  JOURNAL   Genes Dev 9 (21), 2623-2634 (1995)
   PUBMED   7590240
REFERENCE   8  (bases 1 to 3301)
  AUTHORS   Williamson,C.M., Dutton,E.R., Beechey,C.V. and Peters,J.
  TITLE     Protective protein for beta-galactosidase, Ppgb, maps to the distal
            imprinting region of mouse chromosome 2 but is not imprinted
  JOURNAL   Genomics 22 (1), 240-242 (1994)
   PUBMED   7959780
REFERENCE   9  (bases 1 to 3301)
  AUTHORS   Galjart,N.J., Gillemans,N., Meijer,D. and d'Azzo,A.
  TITLE     Mouse 'protective protein'. cDNA cloning, sequence comparison, and
            expression
  JOURNAL   J Biol Chem 265 (8), 4678-4684 (1990)
   PUBMED   2106523
REFERENCE   10 (bases 1 to 3301)
  AUTHORS   Galjart,N.J., Gillemans,N., Harris,A., van der Horst,G.T.,
            Verheijen,F.W., Galjaard,H. and d'Azzo,A.
  TITLE     Expression of cDNA encoding the human 'protective protein'
            associated with lysosomal beta-galactosidase and neuraminidase:
            homology to yeast proteases
  JOURNAL   Cell 54 (6), 755-764 (1988)
   PUBMED   3136930
  REMARK    GeneRIF: characterization of human PPCA, including N-terminal
            sequencing of the two cleavage products
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL591495.12, AK159150.1,
            AK019861.1 and AK032197.1.
            
            On Jan 20, 2013 this sequence version replaced NM_008906.3.
            
            Summary: This gene encodes a glycoprotein with deamidase, esterase
            and carboxypeptidase activities. The encoded protein associates
            with and provides a protective function to the lysosomal enzymes
            beta-galactosidase and neuraminidase. Deficiency of the related
            gene in humans results in galactosialidosis. The proprotein is
            processed into two shorter chains. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene. [provided by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK032197.1, AK159150.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                AL591495.12        16392-16424
            34-1851             AK159150.1         2-1819
            1852-2070           AK019861.1         2057-2275
            2071-3177           AK032197.1         2038-3144
            3178-3301           AL591495.12        23528-23651
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 85.27 cM"
     gene            1..3301
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /note="cathepsin A"
                     /db_xref="GeneID:19025"
                     /db_xref="MGI:MGI:97748"
     exon            1..78
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1503
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /EC_number="3.4.16.5"
                     /note="isoform a preproprotein is encoded by transcript
                     variant 1; lysosomal protective protein; carboxypeptidase
                     C; carboxypeptidase L; protective protein cathepsin A;
                     protective protein for beta-galactosidase"
                     /codon_start=1
                     /product="lysosomal protective protein isoform a
                     preproprotein"
                     /protein_id="NP_032932.2"
                     /db_xref="CCDS:CCDS17061.1"
                     /db_xref="GeneID:19025"
                     /db_xref="MGI:MGI:97748"
                     /translation="MTSSPKAPPGEQGRKEAEMPGTALSPLLLLLLLSWASRNEAAPD
                     QDEIDCLPGLAKQPSFRQYSGYLRASDSKHFHYWFVESQNDPKNSPVVLWLNGGPGCS
                     SLDGLLTEHGPFLIQPDGVTLEYNPYAWNLIANVLYIESPAGVGFSYSDDKMYVTNDT
                     EVAENNYEALKDFFRLFPEYKDNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVG
                     NGLASYEQNDNSLVYFAYYHGLLGNRLWTSLQTHCCAQNKCNFYDNKDPECVNNLLEV
                     SRIVGKSGLNIYNLYAPCAGGVPGRHRYEDTLVVQDFGNIFTRLPLKRRFPEALMRSG
                     DKVRLDPPCTNTTAPSNYLNNPYVRKALHIPESLPRWDMCNFLVNLQYRRLYQSMNSQ
                     YLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVDYGESGEQVA
                     GFVKECSHITFLTIKGAGHMVPTDKPRAAFTMFSRFLNKEPY"
     exon            79..257
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            258..369
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            370..420
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            421..507
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            508..663
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            664..755
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            756..840
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            841..932
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1008
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1148
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1224
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1314
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1419
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..3301
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1831..1836
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /note="hexamer: ATTAAA"
     polyA_site      1853
                     /gene="Ctsa"
                     /gene_synonym="PPCA; Ppgb"
                     /note="major polyA site"
ORIGIN      
        1 atgctcacgt gaccagagct acacatgact tccagtccaa aggcgcctcc tggagagcaa
       61 ggacgcaagg aagcagagat gcccggaacc gcgctgtctc cactgctctt gttgctgctc
      121 ctgtcctggg cgtcccggaa cgaagcagct ccggaccagg atgaaatcga ttgtctcccc
      181 ggcctggcca agcagccctc tttccggcaa tactccggct acctcagagc atcggactcc
      241 aagcacttcc actactggtt tgtggagtcg cagaacgacc caaagaacag ccccgtggtg
      301 ctttggctta acgggggtcc cggctgcagc tcgctcgatg ggctgcttac agagcacggc
      361 ccctttctga tccagccaga tggtgtcacc ctggagtaca acccctatgc ttggaacctg
      421 attgccaacg tgctgtatat cgagtcccca gctggggtgg gcttctccta ctcggatgac
      481 aagatgtacg tgaccaatga cacagaggtg gcggagaaca attatgaagc ccttaaagac
      541 ttcttccgcc tctttccgga atacaaggac aacaaacttt tcctgacagg agagagctat
      601 gctggcatct acatccccac cttggctgta ctggtcatgc aggatcctag catgaatctt
      661 caggggctgg ctgtgggcaa tggacttgcc tcctatgagc agaacgacaa ctccctggtc
      721 tactttgcct actaccatgg ccttctgggg aacagacttt ggacttcact gcagacccac
      781 tgctgcgctc agaacaagtg taacttctat gacaacaaag acccagagtg tgtaaacaat
      841 ctcctggaag tgtctcgaat tgtgggcaaa tctggcctca acatctacaa tctctatgct
      901 ccgtgtgctg gtggggtgcc cggcagacat agatatgagg acacacttgt agtccaggat
      961 tttggcaaca tcttcactcg cctgccactt aagcggagat ttcctgaggc actgatgcgt
     1021 tctggggaca aggtacgctt ggatcctccc tgcaccaaca ccacagcccc ttccaactac
     1081 ctcaacaacc cctatgttcg gaaggctctc cacatccccg agtcgctgcc ccgctgggac
     1141 atgtgcaact tcttggtgaa tttacagtac cgccgcctct accaaagcat gaactcccag
     1201 tacctgaagc tgctcagttc acagaaatac cagatcctgc tctacaacgg agatgtggac
     1261 atggcctgca acttcatggg cgatgagtgg tttgtggatt cgctcaacca gaagatggag
     1321 gtgcagcgcc ggccctggct agtggactac ggggagagcg gagaacaggt agctggtttc
     1381 gtgaaggagt gttcacacat caccttcctc accatcaagg gtgccggaca catggtcccc
     1441 acggacaagc ctcgagctgc ttttaccatg ttctcgaggt tcctgaacaa agagccttac
     1501 tgatagcttc cgctgcaacc catggaaatc ctcagtacct gatcagcccc tctcggcctc
     1561 tctcagcaga gaggagtcct ccgctaatgc tcccgtgggg caagtcctgt actaaaactg
     1621 cccagacttc tcacagcctg gtgcatccca gcctgggcct cagggcgtca cagacagcct
     1681 aggaccaagt gagcactttg ttccggccat cgtggggtgg cctgacctcc ccgactgcat
     1741 aaaaactgac caccccagga ccccaacaga gctagagaaa cccaaggagg ggggtggatg
     1801 gattctaatt gatggttgac taattctgga attaaatcgg gtatagcttc caactgggct
     1861 cttctctgtg gcaccgggac cactaacact gtagatggga aggggttggc agggggtgca
     1921 gagaagactt cagttaggtt cctgaatgcc agccacccag gtggggtgat gggctcaggc
     1981 agctgcagca gagggtgggg ggacatggga ggccgcaacg tctgcaggac ggttcttgtc
     2041 aatcacttct cgaagccctt tggcaaagtg gaggtcagcc ccaacagtga cgaagccctg
     2101 cgggagggga cagggagcct ggctggttca gttgtttgga aacagcctcc aaacaaacac
     2161 aagactcttg agcaaatccc ctcaggggcc ccagctgctt tgcggcgtgg cgtatgttct
     2221 ctggagcatg gccctgcttc taagttaaat ctttactact taaataatat atatgtgaag
     2281 gagcaactag tgtaaagggg tttcgggaaa acagacaagc actggggtgt tgtagggaca
     2341 ggaatgccct ggtctacggc ctcctttcca caatgtactt accgcgtggt tggtcaccac
     2401 ctcacgcacg aagttgatac cctcaggaag ggggatctgc accccacgcc aggtacgctc
     2461 tgtggatgag agaggcaagc tcagcctggg tgtccatcag tccatctgtg tcgtcatgtc
     2521 caccatccac cgccgtcccc cctttgttag tccgtgtccc cactccatcc tcccaccctc
     2581 agtccctacc gttcagcaaa ggcatcactc cgatttgcag cagtgtcttc agtggggcct
     2641 gcagtgggat cagctgaggg ggaaaaaaaa ggcagggggg ggcgacagac agtgcataca
     2701 ggtttgagtg agcggccaac ttgccccgag gcctggaccc accctttgcc atccccaact
     2761 caccgccaga gactccagcg cagactgatt tgagtagatt tgaaacctgt gggagagaaa
     2821 tacaccgtga aacctgggaa caccctttcc ttgggcccta cctaaaagat ggcagcaagt
     2881 gacagccttc ccagttctat ccaatcatcc ttcaggtagc tgccatttcc caggtgtgca
     2941 tccctgagtg cctttggtct ctggagcccc ctttctttaa gcctactgct tttctttttt
     3001 tttttctttt gtcttttttt ttttttttga gacagggctt ctctgtgtag ccctggctgt
     3061 cctggaactc actctgtaga ccaggttggc ctggagctct gaaatccgcc tgcctcagag
     3121 tgctggaatt aaaggcgtgc gccaccactg cccggcgcct gctgtttttc atttgtcagg
     3181 aatgcctatg tgccacgctc tgcggctagg taaataagta tggttttatc taaggaaatt
     3241 ccaacaatct ttactctctg tcagatacca aacttattca aaagaattaa aatggctctg
     3301 c
//
