LOCUS       NM_025583                887 bp    mRNA    linear   ROD 17-DEC-2024
DEFINITION  Mus musculus chymotrypsinogen B1 (Ctrb1), mRNA.
ACCESSION   NM_025583
VERSION     NM_025583.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Demcsak,A., Shariatzadeh,S. and Sahin-Toth,M.
  TITLE     Secretagogue-induced pancreatitis in mice devoid of chymotrypsin
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 327 (3), G333-G344 (2024)
   PUBMED   38981616
  REMARK    GeneRIF: Secretagogue-induced pancreatitis in mice devoid of
            chymotrypsin.
REFERENCE   2  (bases 1 to 887)
  AUTHORS   Cobo,I., Paliwal,S., Bodas,C., Felipe,I., Melia-Aloma,J.,
            Torres,A., Martinez-Villarreal,J., Malumbres,M., Garcia,F.,
            Millan,I., Del Pozo,N., Park,J.C., MacDonald,R.J., Munoz,J.,
            Mendez,R. and Real,F.X.
  TITLE     NFIC regulates ribosomal biology and ER stress in pancreatic acinar
            cells and restrains PDAC initiation
  JOURNAL   Nat Commun 14 (1), 3761 (2023)
   PUBMED   37353485
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 887)
  AUTHORS   Martinez-Ramirez,A.S., Borders,T.L., Paul,L., Schipma,M., Wang,X.,
            Korobova,F., Wright,C.V. and Sosa-Pineda,B.
  TITLE     Specific Temporal Requirement of Prox1 Activity During Pancreatic
            Acinar Cell Development
  JOURNAL   Gastro Hep Adv 1 (5), 807-823 (2022)
   PUBMED   37829188
REFERENCE   4  (bases 1 to 887)
  AUTHORS   Orekhova,A., Nemeth,B.C., Jancso,Z., Geisz,A., Mosztbacher,D.,
            Demcsak,A. and Sahin-Toth,M.
  TITLE     Evolutionary expansion of polyaspartate motif in the activation
            peptide of mouse cationic trypsinogen limits autoactivation and
            protects against pancreatitis
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 321 (6), G719-G734 (2021)
   PUBMED   34643096
REFERENCE   5  (bases 1 to 887)
  AUTHORS   Mosztbacher,D., Jancso,Z. and Sahin-Toth,M.
  TITLE     Loss of chymotrypsin-like protease (CTRL) alters intrapancreatic
            protease activation but not pancreatitis severity in mice
  JOURNAL   Sci Rep 10 (1), 11731 (2020)
   PUBMED   32678161
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 887)
  AUTHORS   Nemeth,B.C., Wartmann,T., Halangk,W. and Sahin-Toth,M.
  TITLE     Autoactivation of mouse trypsinogens is regulated by chymotrypsin C
            via cleavage of the autolysis loop
  JOURNAL   J Biol Chem 288 (33), 24049-24062 (2013)
   PUBMED   23814066
REFERENCE   7  (bases 1 to 887)
  AUTHORS   Chang,I., Bramall,A.N., Baynash,A.G., Rattner,A., Rakheja,D.,
            Post,M., Joza,S., McKerlie,C., Stewart,D.J., McInnes,R.R. and
            Yanagisawa,M.
  TITLE     Endothelin-2 deficiency causes growth retardation, hypothermia, and
            emphysema in mice
  JOURNAL   J Clin Invest 123 (6), 2643-2653 (2013)
   PUBMED   23676500
REFERENCE   8  (bases 1 to 887)
  AUTHORS   Honey,N.K., Sakaguchi,A.Y., Lalley,P.A., Quinto,C., MacDonald,R.J.,
            Craik,C., Bell,G.I., Rutter,W.J. and Naylor,S.L.
  TITLE     Chromosomal assignments of genes for trypsin, chymotrypsin B, and
            elastase in mouse
  JOURNAL   Somat Cell Mol Genet 10 (4), 377-383 (1984)
   PUBMED   6589791
REFERENCE   9  (bases 1 to 887)
  AUTHORS   Watanabe,T., Ogasawara,N. and Goto,H.
  TITLE     Genetic study of pancreatic proteinase in mice (Mus musculus):
            genetic variants of trypsin and chymotrypsin
  JOURNAL   Biochem Genet 14 (9-10), 697-707 (1976)
   PUBMED   1008801
REFERENCE   10 (bases 1 to 887)
  AUTHORS   Watanabe,T. and Tomita,T.S.
  TITLE     Genetic study of pancreatic proteinase and alpha-amylase in mice
            (Mus musculus)
  JOURNAL   Biochem Genet 12 (5), 419-428 (1974)
   PUBMED   4461036
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK007815.1 and AC122364.4.
            
            On Aug 6, 2009 this sequence version replaced NM_025583.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061083.1, AK007566.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-876               AK007815.1         2-877
            877-887             AC122364.4         77570-77580
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 57.98 cM"
     gene            1..887
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /note="chymotrypsinogen B1"
                     /db_xref="GeneID:66473"
                     /db_xref="MGI:MGI:88559"
     exon            1..70
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..810
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /EC_number="3.4.21.1"
                     /codon_start=1
                     /product="chymotrypsinogen B precursor"
                     /protein_id="NP_079859.2"
                     /db_xref="CCDS:CCDS22679.1"
                     /db_xref="GeneID:66473"
                     /db_xref="MGI:MGI:88559"
                     /translation="MAFLWLVSCFALVGATFGCGVPAIQPVLTGLSRIVNGEDAIPGS
                     WPWQVSLQDRTGFHFCGGSLISENWVVTAAHCGVKTTDVVVAGEFDQGSDEENVQVLK
                     IAQVFKNPKFNSFTVRNDITLLKLATPAQFSETVSAVCLPTVDDDFPAGTLCATTGWG
                     KTKYNALKTPDKLQQAALPIVSEAKCKESWGSKITDVMICAGASGVSSCMGDSGGPLV
                     CQKDGVWTLAGIVSWGSGFCSTSTPAVYARVTALMPWVQEILEAN"
     exon            71..174
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /inference="alignment:Splign:2.1.0"
     exon            175..254
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /inference="alignment:Splign:2.1.0"
     exon            255..333
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /inference="alignment:Splign:2.1.0"
     exon            334..514
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /inference="alignment:Splign:2.1.0"
     exon            515..648
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /inference="alignment:Splign:2.1.0"
     exon            649..887
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      859..864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /note="hexamer: AATAAA"
     polyA_site      880
                     /gene="Ctrb1"
                     /gene_synonym="2200008D09Rik; Ctrb; Prt-2"
                     /note="major polyA site"
ORIGIN      
        1 attcccttca ccagcaccat ggcattcctt tggcttgtgt cctgctttgc ccttgtgggc
       61 gccacctttg gctgtggggt ccctgccatc caacccgtgc tgaccggtct gtccaggatc
      121 gtcaacggag aggatgctat ccctggctcc tggccctggc aggtgtccct gcaggacaga
      181 actggcttcc atttctgcgg gggctccctc atcagcgaaa actgggtggt cactgctgcc
      241 cactgcgggg tcaagacaac cgatgtggtg gtagctggag agtttgatca gggctccgat
      301 gaagagaatg tccaggtcct gaaaatcgct caggttttta agaaccccaa gttcaactcc
      361 ttcaccgtgc gtaatgacat caccctgctg aagctggcca ctcctgccca gttctctgag
      421 actgtgtctg ccgtgtgtct gcccactgtt gacgatgact tccctgctgg gacactgtgt
      481 gccaccactg gctggggcaa gaccaaatac aatgccctca agacccctga caagctgcag
      541 caggctgccc tgcccatcgt gtccgaggct aagtgcaagg agtcctgggg ttccaagatc
      601 accgatgtga tgatctgcgc aggcgccagt ggcgtctctt cctgcatggg tgactctggt
      661 ggccccctcg tctgccagaa ggacggagtc tggaccctgg caggcattgt gtcctggggc
      721 agtggtttct gctccacttc cacccctgcc gtgtatgccc gagtcactgc cctcatgccc
      781 tgggttcagg agatcttgga agccaactaa gtacacggtc ctttcctctt ccctcttcct
      841 ccccctccgt gcatctacaa taaaaatcat ctaattagaa cattgac
//
