LOCUS       NM_145953               1815 bp    mRNA    linear   ROD 26-NOV-2024
DEFINITION  Mus musculus cystathionine gamma lyase (Cth), mRNA.
ACCESSION   NM_145953
VERSION     NM_145953.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Hu,Y.T., Liu,Z.W., Zhang,T.H., Ma,Y.E., He,L., Zhang,J., Zhou,Y.Y.,
            Vidal-Puig,A., Pan,D.J. and Wu,F.
  TITLE     Cystathionine gamma-lyase-derived H2S negatively regulates thymic
            egress via allosteric inhibition of sphingosine-1-phosphate lyase
  JOURNAL   Acta Pharmacol Sin 45 (11), 2366-2379 (2024)
   PUBMED   38914678
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Gao,S., Li,Y., Liu,M.M., Xiong,X., Cui,C.P., Huo,Q.J., Li,K.X.,
            Sun,X., Zhang,R., Wu,D. and Li,B.Y.
  TITLE     The crucial relationship between miRNA-27 and CSE/H2S, and the
            mechanism of action of GLP-1 in myocardial hypertrophy
  JOURNAL   Int J Med Sci 21 (5), 965-977 (2024)
   PUBMED   38616996
  REMARK    GeneRIF: The crucial relationship between miRNA-27 and CSE/H2S, and
            the mechanism of action of GLP-1 in myocardial hypertrophy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1815)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1815)
  AUTHORS   Drekolia,M.K., Karantanou,C., Wittig,I., Li,Y., Fuhrmann,D.C.,
            Brune,B., Katsouda,A., Hu,J., Papapetropoulos,A. and Bibli,S.I.
  TITLE     Loss of cardiac mitochondrial complex I persulfidation impairs NAD+
            homeostasis in aging
  JOURNAL   Redox Biol 69, 103014 (2024)
   PUBMED   38171255
REFERENCE   5  (bases 1 to 1815)
  AUTHORS   Shentu,Y., Chen,M., Wang,H., Du,X., Zhang,W., Xie,G., Zhou,S.,
            Ding,L., Zhu,Y., Zhu,M., Zhang,N., Du,C., Ma,J., Chen,R., Yang,J.,
            Fan,X., Gong,Y., Zhang,H. and Fan,J.
  TITLE     Hydrogen sulfide ameliorates lipopolysaccharide-induced
            anxiety-like behavior by inhibiting checkpoint kinase 1 activation
            in the hippocampus of mice
  JOURNAL   Exp Neurol 371, 114586 (2024)
   PUBMED   37898396
REFERENCE   6  (bases 1 to 1815)
  AUTHORS   Yang,G., Wu,L., Jiang,B., Yang,W., Qi,J., Cao,K., Meng,Q.,
            Mustafa,A.K., Mu,W., Zhang,S., Snyder,S.H. and Wang,R.
  TITLE     H2S as a physiologic vasorelaxant: hypertension in mice with
            deletion of cystathionine gamma-lyase
  JOURNAL   Science 322 (5901), 587-590 (2008)
   PUBMED   18948540
  REMARK    GeneRIF: study shows H2S is physiologically generated by
            cystathionine gamma-lyase (CSE) & genetic deletion CSE markedly
            reduces H2S levels in serum & tissue; mice lacking CSE display
            pronounced hypertension & diminished endothelium-dependent
            vasorelaxation
REFERENCE   7  (bases 1 to 1815)
  AUTHORS   Pong,W.W., Stouracova,R., Frank,N., Kraus,J.P. and Eldred,W.D.
  TITLE     Comparative localization of cystathionine beta-synthase and
            cystathionine gamma-lyase in retina: differences between amphibians
            and mammals
  JOURNAL   J Comp Neurol 505 (2), 158-165 (2007)
   PUBMED   17853447
  REMARK    GeneRIF: Te CGL antiserum recognized the correct molecular weight
            protein for mouse retina.
REFERENCE   8  (bases 1 to 1815)
  AUTHORS   Tamizhselvi,R., Moore,P.K. and Bhatia,M.
  TITLE     Hydrogen sulfide acts as a mediator of inflammation in acute
            pancreatitis: in vitro studies using isolated mouse pancreatic
            acinar cells
  JOURNAL   J Cell Mol Med 11 (2), 315-326 (2007)
   PUBMED   17488480
  REMARK    GeneRIF: Caerulein increased the level of cystathionine
            gamma-Lyase.
REFERENCE   9  (bases 1 to 1815)
  AUTHORS   Okuno,T., Motobayashi,S., Ueno,H. and Nakamuro,K.
  TITLE     Identification of mouse selenomethionine alpha,gamma-elimination
            enzyme: cystathionine gamma-lyase catalyzes its reaction to
            generate methylselenol
  JOURNAL   Biol Trace Elem Res 108 (1-3), 245-257 (2005)
   PUBMED   16327076
  REMARK    GeneRIF: The purpose of this study was to identify the
            seleno-L-methionine (L-SeMet) alpha,gamma-elimination enzyme that
            catalyzes L-SeMet to generate methylselenol (CH3SeH)
REFERENCE   10 (bases 1 to 1815)
  AUTHORS   Ishii,I., Akahoshi,N., Yu,X.N., Kobayashi,Y., Namekata,K.,
            Komaki,G. and Kimura,H.
  TITLE     Murine cystathionine gamma-lyase: complete cDNA and genomic
            sequences, promoter activity, tissue distribution and developmental
            expression
  JOURNAL   Biochem J 381 (Pt 1), 113-123 (2004)
   PUBMED   15038791
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY083352.1.
            
            On Jun 19, 2003 this sequence version replaced NM_145953.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY083352.1, AK002480.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 81.55 cM"
     gene            1..1815
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /note="cystathionine gamma lyase"
                     /db_xref="GeneID:107869"
                     /db_xref="MGI:MGI:1339968"
     exon            1..198
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..1230
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /EC_number="4.4.1.1"
                     /EC_number="4.4.1.2"
                     /note="cysteine-protein sulfhydrase; cysteine
                     desulfhydrase; homocysteine desulfhydrase; cystathionase
                     (cystathionine gamma-lyase)"
                     /codon_start=1
                     /product="cystathionine gamma-lyase"
                     /protein_id="NP_666065.1"
                     /db_xref="CCDS:CCDS51101.1"
                     /db_xref="GeneID:107869"
                     /db_xref="MGI:MGI:1339968"
                     /translation="MQKDASLSGFLPSFQHFATQAIHVGQEPEQWNSRAVVLPISLAT
                     TFKQDFPGQSSGFEYSRSGNPTRNCLEKAVAALDGAKHSLAFASGLAATITITHLLKA
                     GDEIICMDEVYGGTNRYFRRVASEFGLKISFVDCSKTKLLEAAITPQTKLVWIETPTN
                     PTLKLADIGACAQIVHKRGDIILVVDNTFMSAYFQRPLALGADICMCSATKYMNGHSD
                     VVMGLVSVNSDDLNSRLRFLQNSLGAVPSPFDCYLCCRGLKTLQVRMEKHFKNGMAVA
                     RFLETNPRVEKVVYPGLPSHPQHELAKRQCSGCPGMVSFYIKGALQHAKAFLKNLKLF
                     TLAESLGGYESLAELPAIMTHASVPEKDRATLGINDTLIRLSVGLEDEQDLLEDLDRA
                     LKAAHP"
     exon            199..280
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            281..376
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            377..486
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            487..618
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            619..676
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            677..754
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            755..907
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1029
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1082
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1221
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1801
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1779..1784
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /note="hexamer: AATAAA"
     polyA_site      1801
                     /gene="Cth"
                     /gene_synonym="0610010I13Rik; CGL; CSE; Cys3"
                     /note="major polyA site"
ORIGIN      
        1 agccaaagca acacctcgca ctcctgcccc agcatgcaga aggacgcctc tttgagcggc
       61 ttcctgccta gtttccagca tttcgccact caggccatcc acgtgggaca agagcctgag
      121 caatggaatt ctcgtgccgt ggtgctgccc atttcgttgg ccaccacatt taagcaggac
      181 ttcccgggcc agtcctcggg ttttgaatac agccgctctg gaaatccaac aaggaattgc
      241 ttggaaaaag cagtggctgc gttggatggg gcaaagcaca gtttggcctt tgcatcgggt
      301 cttgctgcca ccattacgat tacccatctt ttaaaagcag gagatgaaat catttgcatg
      361 gatgaagtgt atggaggcac caacaggtac ttcaggaggg tggcatctga atttggactg
      421 aagatttctt ttgtagattg ttccaaaacc aaattgctag aggcagcgat tacaccacaa
      481 accaagcttg tttggatcga aacacccaca aacccaactt tgaagttggc tgacattgga
      541 gcctgcgcac aaattgtcca caaacgtgga gacatcattt tggttgtaga taacaccttc
      601 atgtctgcat atttccagag acctttggct ctgggtgctg atatttgtat gtgttctgcc
      661 acaaaataca tgaatggcca cagcgatgtt gtcatgggtt tagtgtctgt taattctgat
      721 gacctcaata gtcggcttcg tttcctgcag aattcactag gagcagttcc ttctcctttt
      781 gattgttacc tctgctgccg aggcctgaag acactgcagg tccggatgga gaaacatttc
      841 aagaatggga tggcggtggc tcgtttcctg gagaccaatc cccgggtaga aaaggttgtt
      901 tatcctgggc taccctctca ccctcagcat gagctggcca aacgccagtg ctcgggctgc
      961 ccagggatgg tcagtttcta catcaagggt gctctgcagc atgctaaggc cttcctcaaa
     1021 aatctaaagc tgtttactct ggcagagagc ctgggaggat atgagagtct ggctgagctt
     1081 ccagcaatca tgacccatgc ctctgtgcct gagaaggaca gagctaccct cgggatcaat
     1141 gacacactga tacgactttc tgtgggccta gaggatgaac aggaccttct tgaagacctg
     1201 gatcgagctt tgaaggcagc gcacccttaa aagttcgagt caaagctgtc attccagtgc
     1261 tgccatcagg agcagcatcc aaggggccaa atcttcggaa taactggaca gatcattacg
     1321 gagcatacgc agaactgcac tgaatatttt aaggccctaa tgagtttaca gctgtaacct
     1381 tccatggatc ttcccttaag aactgtcttc tgtttatctt ctaactaaca ggttgttctg
     1441 ttagtatcat ttcggtaatt ttgctatatt tgtgtccaag gaagtaagag ttgttctgtt
     1501 ttgggatcat gttgtctttt ttctttcttc agtagcttat gatatatttt aatcatgttt
     1561 acaatgttac aatttagtat tgatgtttta tgaagttaaa ttattaaatg aatggtctta
     1621 aatccactgt ggtggttttt ttttgaaaaa ttatataatt accataagcc aaaaatcaaa
     1681 tatttggaat acctactgtg aaattcaaga gattaaaggt tgtacttgat acttgttatt
     1741 tttcttaaat aaatctagtt atcaagttat ttttcttaaa taaatctagt tatcaaatgt
     1801 aaaaaaaaaa aaaaa
//
