LOCUS       NM_177655                666 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus cystatin-like 1 (Cstl1), transcript variant 2, mRNA.
ACCESSION   NM_177655 NM_001004763 NM_001004764
VERSION     NM_177655.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Xiang,Y., Nie,D.S., Zhang,Q.J., Zhu,W.B., Du,J., Li,W. and Lu,G.X.
  TITLE     Cloning, characterization and identification of Rcet1-v1 and
            Rcet1-v2, two novel splice variants of mouse Rcet1 related to Cres
            subgroup of family 2 cystatins
  JOURNAL   DNA Seq 19 (1), 13-19 (2008)
   PUBMED   18300157
  REMARK    GeneRIF: Primarily expressed in mouse male reproductive tract; may
            play important roles in mouse spermatocyte and spermatid
            development.
REFERENCE   2  (bases 1 to 666)
  AUTHORS   Frygelius,J., Oscarson,M., Nordqvist,K., Wedell,A. and Tohonen,V.
  TITLE     The reproductive tissue specific cystatin subgroup of genes:
            expression during gonadal development in wildtype and testatin
            knockout animals
  JOURNAL   Sex Dev 1 (6), 363-372 (2007)
   PUBMED   18391548
REFERENCE   3  (bases 1 to 666)
  AUTHORS   Yasothornsrikul,S., Greenbaum,D., Medzihradszky,K.F., Toneff,T.,
            Bundey,R., Miller,R., Schilling,B., Petermann,I., Dehnert,J.,
            Logvinova,A., Goldsmith,P., Neveu,J.M., Lane,W.S., Gibson,B.,
            Reinheckel,T., Peters,C., Bogyo,M. and Hook,V.
  TITLE     Cathepsin L in secretory vesicles functions as a
            prohormone-processing enzyme for production of the enkephalin
            peptide neurotransmitter
  JOURNAL   Proc Natl Acad Sci U S A 100 (16), 9590-9595 (2003)
   PUBMED   12869695
REFERENCE   4  (bases 1 to 666)
  AUTHORS   Cornwall,G.A. and Hsia,N.
  TITLE     A new subgroup of the family 2 cystatins
  JOURNAL   Mol Cell Endocrinol 200 (1-2), 1-8 (2003)
   PUBMED   12644294
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844519.9.
            
            On Apr 7, 2018 this sequence version replaced NM_177655.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BY707337.1, AK131797.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                AL844519.9         24230-24269
            41-384              AL844519.9         24680-25023
            385-495             AL844519.9         28476-28586
            496-666             AL844519.9         29133-29303
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 73.53 cM"
     gene            1..666
                     /gene="Cstl1"
                     /gene_synonym="RCET1; RCET2; RCET3"
                     /note="cystatin-like 1"
                     /db_xref="GeneID:228756"
                     /db_xref="MGI:MGI:2652834"
     exon            1..40
                     /gene="Cstl1"
                     /gene_synonym="RCET1; RCET2; RCET3"
                     /inference="alignment:Splign:2.1.0"
     exon            41..384
                     /gene="Cstl1"
                     /gene_synonym="RCET1; RCET2; RCET3"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..579
                     /gene="Cstl1"
                     /gene_synonym="RCET1; RCET2; RCET3"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="cystatin-like 1 isoform 2 precursor"
                     /protein_id="NP_808323.1"
                     /db_xref="CCDS:CCDS16844.1"
                     /db_xref="GeneID:228756"
                     /db_xref="MGI:MGI:2652834"
                     /translation="MEMKARGLRIPLLLLLVTVVVMAKVNHIQRWGGFKEKAMSKKNI
                     NSTLHFFIRSYNNASNDTYLYQVQKLIQGQMQLTTGVEYLVTVKIGRTKCKKNETKKA
                     SCPLQSSKLKKSLICKSLIYSVPWMNYYQLWNNSCQES"
     exon            385..495
                     /gene="Cstl1"
                     /gene_synonym="RCET1; RCET2; RCET3"
                     /inference="alignment:Splign:2.1.0"
     exon            496..666
                     /gene="Cstl1"
                     /gene_synonym="RCET1; RCET2; RCET3"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      610
                     /gene="Cstl1"
                     /gene_synonym="RCET1; RCET2; RCET3"
                     /note="major polyA site"
ORIGIN      
        1 gggaaacagc agagagaaat ctgtggcagg taggaggcag ggtccgtgaa gctgtctccc
       61 aggaagccaa tgccggcaag actgctgtgg aaggcggaga gaactgttgc tgcactcgaa
      121 agctggcagc actgcagcta agtgccttcc gaagccatgg agatgaaggc cagaggtttg
      181 aggatcccct tgctgctgtt gctggtgact gtggtcgtaa tggctaaggt gaatcacatc
      241 cagaggtggg ggggtttcaa ggagaaggcc atgagcaaga aaaacatcaa ctcgacactc
      301 cattttttca tccgctctta caacaacgca agcaacgaca cctacttgta tcaagttcag
      361 aagctaattc aaggtcagat gcagttgacc acaggggtgg agtatttggt tactgtgaag
      421 attggcagga ccaaatgtaa gaaaaatgag acgaagaaag cctcctgccc cctgcaaagc
      481 agcaagctga aaaagagcct gatttgcaaa tcactgatat actctgtacc ctggatgaac
      541 tactaccagc tctggaacaa ttcctgccag gagagctgag tgtctatgta aagagctctg
      601 ctgacaccca gatgcctgtg gtgtgtactg gttgtactgc ctgtggtgtg taaaattgta
      661 agagga
//
